LOCUS       pCH1/RAD51Opt              6645 bp    DNA     circular     19-MAR-1980
DEFINITION  pCOLADuet-1/GroE/RAD51-Opt.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCH1-RAD51Opt|
COMMENT     VNTAUTHORNAME|Shyamal Subramanyam|
COMMENT     VNTREPLTYPE|Plasmid
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FEATURES             Location/Qualifiers
     rep_origin      4590..5225
                     /vntifkey="33"
                     /label=ColA\Ori
                     /note="ColA Replication Origin"
     CDS             complement(5423..6505)
                     /vntifkey="4"
                     /label=LacI
                     /note="WildType LacI Gene"
     promoter        1156..1172
                     /vntifkey="30"
                     /label=T7\Promoter
                     /note="T7 Promoter"
     promoter        6629..6645
                     /vntifkey="30"
                     /label=T7\Promoter
                     /note="T7 Promoter"
     CDS             complement(3660..4480)
                     /vntifkey="4"
                     /label=KanR
                     /note="Neomycin Phosphotransferase II [NPT II/Neo]"
     operon          1241..3226
                     /vntifkey="103"
                     /label=GroE\Operon
                     /note="E.Coli GroE Operon [X07850.1]"
     protein_bind    5..25
                     /vntifkey="31"
                     /label=LacO
                     /note="LacO"
     protein_bind    1177..1197
                     /vntifkey="31"
                     /label=LacO
                     /note="LacO"
     RBS             1228..1233
                     /vntifkey="32"
                     /label=RBS-2
                     /note="Ribosome Binding Site"
     RBS             58..63
                     /vntifkey="32"
                     /label=RBS-1
                     /note="Ribosome Binding Site"
     misc_feature    1564..1569
                     /vntifkey="21"
                     /label=RBS-3
                     /note="RBS-3 similar to E. coli galT RBS"
     CDS             1242..1535
                     /vntifkey="4"
                     /label=GroES
                     /note="Cpn10 Chaperonin GroES, Small Subunit of GroESL [Escherichia coli str. K-12 substr. MG1655] (NP_418566)"
     CDS             1579..3225
                     /vntifkey="4"
                     /label=GroEL
                     /note="Chaperonin GroEL [Escherichia coli DH1] (ACX41451.1)"
     protein_bind    3287..3331
                     /vntifkey="31"
                     /label=S.Tag
                     /note="S.Tag"
     misc_feature    4522..4528
                     /vntifkey="21"
                     /label=-10\Ap
     misc_feature    4545..4550
                     /vntifkey="21"
                     /label=-35\Ap
     primer          complement(3369..3387)
                     /vntifkey="27"
                     /label=T7\Terminator\Primer
                     /note="T7 Terminator Primer"
     terminator      3383..3430
                     /vntifkey="43"
                     /label=T7\Terminator
                     /note="T7 Terminator"
     primer_bind     complement(1635..1658)
                     /vntifkey="28"
                     /label=GROE\RP3
                     /note="GroE RP3 Primer Binding Site"
     primer_bind     2180..2204
                     /vntifkey="28"
                     /label=GROE\RP2
                     /note="GroE RP2 Primer Binding Site"
     primer_bind     2822..2843
                     /vntifkey="28"
                     /label=GROE\RP1
                     /note="GroE RP1 Primer Binding Site"
     CDS             71..1090
                     /vntifkey="4"
                     /label=RAD51\Opt
                     /note="Human RAD51 Codon Optimized for E.coli Expression"
     primer          457..475
                     /vntifkey="27"
                     /label=RAD51o\F1
                     /note="Internal Forward Sequencing Primer"
     primer          600..621
                     /vntifkey="27"
                     /label=RAD51o\R1
                     /note="Internal Reverse Sequencing Primer"
     primer          1127..1151
                     /vntifkey="27"
                     /label=COLAORF2\FP/RP
                     /note="Forward and Reverse Vector Primers for Sequencing "
BASE COUNT     1732 a      1668 c      1715 g      1530 t 
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 
       61 gagatatacc atggcaatgc agatgcagct ggaagcgaac gcagatacct cagtggaaga 
      121 agaaagtttt ggtccgcaac cgattagccg cctggaacag tgcggtatca acgcaaatga 
      181 tgtgaaaaag ctggaagaag ctggctttca taccgttgaa gcagtcgctt atgcgccgaa 
      241 aaaggaactg atcaacatca agggtatcag cgaagccaaa gcagacaaga tcctggccga 
      301 agcggccaaa ctggttccga tgggctttac cacggcaacc gaatttcatc agcgtcgcag 
      361 cgaaattatc caaattacca cgggttctaa agaactggat aagctgctgc agggcggtat 
      421 tgaaaccggc tctatcacgg aaatgtttgg tgaatttcgc accggcaaaa cgcagatttg 
      481 ccacaccctg gcggtgacgt gtcaactgcc gatcgatcgt ggcggtggcg agggtaaagc 
      541 catgtacatt gacaccgaag gcacgtttcg tccggaacgc ctgctggctg ttgcggaacg 
      601 ctatggtctg agcggctctg atgtgctgga caacgttgcc tacgcacgtg ctttcaatac 
      661 cgatcatcag acgcaactgc tgtatcaggc gagtgccatg atggtcgaat cccgctacgc 
      721 gctgctgatt gtggatagcg caaccgctct gtatcgtacg gactactcag gtcgcggcga 
      781 actgtcggca cgtcaaatgc acctggctcg ttttctgcgc atgctgctgc gtctggcgga 
      841 cgaatttggt gttgccgtgg ttatcaccaa ccaggtcgtg gcgcaagtcg atggcgcagc 
      901 tatgttcgcg gccgacccga aaaagccgat tggtggcaat attatcgcgc acgccagtac 
      961 cacgcgcctg tatctgcgta aaggtcgcgg cgaaacccgt atttgcaaaa tctatgattc 
     1021 cccgtgtctg ccggaagccg aagccatgtt tgccatcaat gccgacggtg ttggtgacgc 
     1081 taaggattaa gcggccgcat aatgcttaag tcgaacagaa agtaatcgta ttgtacacgg 
     1141 ccgcataatc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc 
     1201 catcttagta tattagttaa gtataagaag gagatataca tatgaatatt cgtccattgc 
     1261 atgatcgcgt gatcgtcaag cgtaaagaag ttgaaactaa atctgctggc ggcatcgttc 
     1321 tgaccggctc tgcagcggct aaatccaccc gcggcgaagt gctggctgtc ggcaatggcc 
     1381 gtatccttga aaatggcgaa gtgaagccgc tggatgtgaa agttggcgac atcgttattt 
     1441 tcaacgatgg ctacggtgtg aaatctgaga agatcgacaa tgaagaagtg ttgatcatgt 
     1501 ccgaaagcga cattctggca attgttgaag cgtaatccgc gcacgacact gaacatacga 
     1561 atttaaggaa taaagataat ggcagctaaa gacgtaaaat tcggtaacga cgctcgtgtg 
     1621 aaaatgctgc gcggcgtaaa cgtactggca gatgcagtga aagttaccct cggtccaaaa 
     1681 ggccgtaacg tagttctgga taaatctttc ggtgcaccga ccatcaccaa agatggtgtt 
     1741 tccgttgctc gtgaaatcga actggaagac aagttcgaaa atatgggtgc gcagatggtg 
     1801 aaagaagttg cctctaaagc aaacgacgct gcaggcgacg gtaccaccac tgcaaccgta 
     1861 ctggctcagg ctatcatcac tgaaggtctg aaagctgttg ctgcgggcat gaacccgatg 
     1921 gacctgaaac gtggtatcga caaagcggtt accgttgcag ttgaagaact gaaagcgctg 
     1981 tccgtaccat gctctgactc taaagcgatt gctcaggttg gtaccatctc cgctaactcc 
     2041 gacgaaaccg taggtaaact gatcgctgaa gcgatggaca aagtcggtaa agaaggcgtt 
     2101 atcaccgttg aagacggtac cggtctgcag gacgaactgg acgtggttga aggtatgcag 
     2161 ttcgaccgtg gctacctgtc tccttacttc atcaacaagc cggaaactgg cgcagtagaa 
     2221 ctggaaagcc cgttcatcct gctggctgac aagaaaatct ccaacatccg cgaaatgctg 
     2281 ccggttctgg aagctgttgc caaagcaggc aaaccgctgc tgatcatcgc tgaagatgta 
     2341 gaaggcgaag cgctggcaac tctggttgtt aacaccatgc gtggcatcgt gaaagtcgct 
     2401 gcggttaaag caccgggctt cggcgatcgt cgtaaagcta tgctgcagga tatcgcaacc 
     2461 ctgactggcg gtaccgtgat ctctgaagag atcggtatgg agctggaaaa agcaaccctg 
     2521 gaagacctgg gtcaggctaa acgtgttgtg atcaacaaag acaccaccac tatcatcgat 
     2581 ggcgtgggtg aagaagctgc aatccagggc cgtgttgctc agatccgtca gcagattgaa 
     2641 gaagcaactt ctgactacga ccgtgaaaaa ctgcaggaac gcgtagcgaa actggcaggc 
     2701 ggcgttgcag ttatcaaagt gggtgctgct accgaagttg aaatgaaaga gaaaaaagca 
     2761 cgcgttgaag atgccctgca cgcgacccgt gctgcggtag aagaaggcgt ggttgctggt 
     2821 ggtggtgttg cgctgatccg cgtagcgtct aaactggctg acctgcgtgg tcagaacgaa 
     2881 gaccagaacg tgggtatcaa agttgcactg cgtgcaatgg aagctccgct gcgtcagatc 
     2941 gtattgaact gcggcgaaga accgtctgtt gttgctaaca ccgttaaagg cggcgacggc 
     3001 aactacggtt acaacgcagc aaccgaagaa tacggcaaca tgatcgacat gggtatcctg 
     3061 gatccaacca aagtaactcg ttctgctctg cagtacgcag cttctgtggc tggcctgatg 
     3121 atcaccaccg aatgcatggt taccgacctg ccgaaaaacg atgcagctga cttaggcgct 
     3181 gctggcggta tgggcggcat gggtggcatg ggcggcatga tgtaaagatc tcaattggat 
     3241 atcggccggc cacgcgatcg ctgacgtcgg taccctcgag tctggtaaag aaaccgctgc 
     3301 tgcgaaattt gaacgccagc acatggactc gtctactagc gcagcttaat taacctaggc 
     3361 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag 
     3421 gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt 
     3481 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc 
     3541 gacgacaagc tgacgaccgg gtctccgcaa gtggcacttt tcggggaaat gtgcgcggaa 
     3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct 
     3661 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata 
     3721 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat 
     3781 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct 
     3841 attaagccgc tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga 
     3901 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag 
     3961 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg 
     4021 attgcgcctg agcgagacga aatacgcggt cgctgttaaa aggacaatta caaacaggaa 
     4081 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag 
     4141 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg 
     4201 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc 
     4261 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca 
     4321 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc 
     4381 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc 
     4441 gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt 
     4501 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     4561 aaaataaaca aataggcatg ctagcgcaga aacgtcctag aagatgccag gaggatactt 
     4621 agcagagaga caataaggcc ggagcgaagc cgtttttcca taggctccgc ccccctgacg 
     4681 aacatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat 
     4741 accaggcgtt tccccctgat ggctccctct tgcgctctcc tgttcccgtc ctgcggcgtc 
     4801 cgtgttgtgg tggaggcttt acccaaatca ccacgtcccg ttccgtgtag acagttcgct 
     4861 ccaagctggg ctgtgtgcaa gaaccccccg ttcagcccga ctgctgcgcc ttatccggta 
     4921 actatcatct tgagtccaac ccggaaagac acgacaaaac gccactggca gcagccattg 
     4981 gtaactgaga attagtggat ttagatatcg agagtcttga agtggtggcc taacagaggc 
     5041 tacactgaaa ggacagtatt tggtatctgc gctccactaa agccagttac caggttaagc 
     5101 agttccccaa ctgacttaac cttcgatcaa accgcctccc caggcggttt tttcgtttac 
     5161 agagcaggag attacgacga tcgtaaaagg atctcaagaa gatcctttac ggattcccga 
     5221 caccatcact ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc 
     5281 ccatacgata taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct 
     5341 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta 
     5401 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     5461 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg 
     5521 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct 
     5581 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga 
     5641 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg 
     5701 agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca 
     5761 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg 
     5821 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt 
     5881 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta 
     5941 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca 
     6001 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat 
     6061 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat 
     6121 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg 
     6181 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt 
     6241 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg 
     6301 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg 
     6361 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt 
     6421 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga 
     6481 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct 
     6541 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct 
     6601 cccttatgcg actcctgcat taggaaatta atacgactca ctata 
//
