LOCUS       pQP_T2A                 8184 bp    CIRCULAR             26-SEP-2017
UNIMARK     pQP_T2A annotations
            pQP_T2A
FEATURES             Location/Qualifiers
     misc_feature    159..681
                     /ugene_name="CMV"
     misc_feature    695..2887
                     /ugene_name="BphP1"
     misc_feature    2930..3313
                     /ugene_name="VP16"
     misc_feature    3323..3376
                     /ugene_name="T2A"
     misc_feature    3380..3397
                     /ugene_name="NLS"
     misc_feature    3413..3853
                     /ugene_name="GAL4"
     misc_feature    3860..4378
                     /ugene_name="QPAS1"
     misc_feature    complement(6270..7200)
                     /ugene_name="Amp"
     misc_feature    complement(7348..7991)
                     /ugene_name="pUC ori"
ORIGIN
        1 CATTAATGCA GCAGCTGCGC GCTCGCTCGC TCACTGAGGC CGCCCGGGCA AAGCCCGGGC
       61 GTCGGGCGAC CTTTGGTCGC CCGGCCTCAG TGAGCGAGCG AGCGCGCAGA GAGGGAGTGG
      121 CCAACTCCAT CACTAGGGGT TCCTTGTAGT TACCTAGGCG TTACATAACT TACGGTAAAT
      181 GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT
      241 CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA
      301 ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
      361 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT
      421 ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG
      481 TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT
      541 GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC
      601 AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
      661 AGAGCTCGTT TAGTGAACCG TCAAGCTTAC CATGGTGGCA GGTCATGCCT CTGGCAGCCC
      721 CGCATTCGGG ACCGCCGATC TTTCGAATTG CGAACGTGAA GAGATCCACC TCGCCGGCTC
      781 GATCCAGCCG CATGGCGCGC TTCTGGTCGT CAGCGAGCCG GATCATCGCA TCATCCAGGC
      841 CAGCGCCAAC GCCGCGGAAT TTCTGAATCT CGGAAGCGTG CTCGGCGTTC CGCTCGCCGA
      901 GATCGACGGC GATCTGTTGA TCAAGATCCT GCCGCATCTC GATCCCACCG CCGAAGGCAT
      961 GCCGGTCGCG GTGCGCTGCC GGATCGGCAA TCCCTCCACG GAGTACGACG GTCTGATGCA
     1021 TCGGCCTCCG GAAGGCGGGC TGATCATCGA GCTCGAACGT GCCGGCCCGC CGATCGATCT
     1081 GTCCGGCACG CTGGCGCCGG CGCTGGAGCG GATCCGCACG GCGGGCTCGC TGCGCGCGCT
     1141 GTGCGATGAC ACCGCGCTGC TGTTTCAGCA GTGCACCGGC TACGACCGGG TGATGGTGTA
     1201 TCGCTTCGAC GAGCAGGGCC ACGGCGAAGT GTTCTCCGAG CGCCACGTGC CCGGGCTCGA
     1261 ATCCTATTTC GGCAACCGCT ATCCGTCGTC GGACATTCCG CAGATGGCGC GGCGGCTGTA
     1321 CGAGCGGCAG CGCGTCCGCG TGCTGGTCGA CGTCAGCTAT CAGCCGGTGC CGCTGGAGCC
     1381 GCGGCTGTCG CCGCTGACCG GGCGCGATCT CGACATGTCG GGCTGCTTCC TGCGCTCGAT
     1441 GTCGCCGATC CATCTGCAGT ACCTGAAGAA CATGGGCGTG CGCGCCACCC TGGTGGTGTC
     1501 GCTGGTGGTC GGCGGCAAGC TGTGGGGCCT GGTTGCCTGT CATCATTATC TGCCGCGCTT
     1561 CATGCATTTC GAGCTGCGGG CGATCTGCGA ACTGCTCGCC GAAGCGATCG CGACGCGGAT
     1621 CACCGCGCTT GAGAGCTTCG CGCAGAGCCA GTCGGAGCTG TTCGTGCAGC GGCTCGAACA
     1681 GCGCATGATC GAAGCGATTA CCCGTGAAGG CGATTGGCGC GCAGCGATTT TCGACACCAG
     1741 CCAATCGATC CTGCAGCCGC TGCACGCCGC CGGTTGCGCG CTGGTGTACG AAGACCAGAT
     1801 CAGGACCATC GGCGACGTGC CTTCCACGCA GGATGTGCGC GAGATCGCCG GGTGGCTCGA
     1861 TCGCCAGCCG CGCGCGGCGG TGACCTCGAC CGCGTCGCTC GGTCTCGACG TGCCGGAGCT
     1921 CGCGCATCTG ACGCGGATGG CGAGCGGCGT GGTCGCGGCG CCGATTTCGG ATCATCGCGG
     1981 CGAGTTTCTG ATGTGGTTCC GCCCCGAGCG CGTCCACACC GTTACCTGGG GCGGCGATCC
     2041 GAAGAAGCCG TTCACGATGG GCGATACACC GGCGGATCTG TCGCCGCGGC GCTCCTTCGC
     2101 CAAATGGCAT CAGGTTGTCG AAGGCACGTC CGATCCGTGG ACGGCCGCCG ATCTCGCCGC
     2161 GGCTCGCACC ATCGGTCAGA CCGTCGCCGA CATCGTGCTG CAATTCCGCG CGGTGCGGAC
     2221 ACTGATCGCC CGCGAACAGT ACGAACAGTT TTCGTCCCAG GTGCACGCTT CGATGCAGCC
     2281 GGTGCTGATC ACCGACGCCG AAGGCCGCAT CCTGCTGATG AACGACTCGT TCCGCGACAT
     2341 GTTGCCGGCG GGTTCGCCAT CCGCCGTCCA TCTCGACGAT CTCGCCGGGT TCTTCGTCGA
     2401 ATCGAACGAT TTCCTGCGCA ACGTCGCCGA ACTGATCGAT CACGGCCGCG GGTGGCGCGG
     2461 CGAAGTTCTG CTGCGCGGCG CAGGCAACCG CCCGTTGCCG CTGGCAGTGC GCGCCGATCC
     2521 GGTGACGCGC ACGGAGGACC AGTCGCTCGG CTTCGTGCTG ATCTTCAGCG ACGCTACCGA
     2581 TCGTCGCACC GCAGATGCCG CACGCACGCG TTTCCAGGAA GGCATTCTTG CCAGCGCACG
     2641 TCCCGGCGTG CGGCTCGACT CCAAGTCCGA CCTGTTGCAC GAGAAGCTGC TGTCCGCGCT
     2701 GGTCGAGAAC GCGCAGCTTG CCGCATTGGA AATCACTTAC GGCGTCGAGA CCGGACGCAT
     2761 CGCCGAGCTG CTCGAAGGCG TCCGCCAGTC GATGCTGCGC ACCGCCGAAG TGCTCGGCCA
     2821 TCTGGTGCAG CACGCGGCGC GCACGGCCGG CAGCGACAGC TCGAGCAATG GCTCGCAGAA
     2881 CAAGAAGGAA TTCGATAGTG CTGGTAGTGC TGGTAGTGCT GGTACTAGAG CGTACAGCCG
     2941 CGCGCGTACG AAAAACAATT ACGGGTCTAC CATCGAGGGC CTGCTCGATC TCCCGGACGA
     3001 CGACGCCCCC GAAGAGGCGG GGCTGGCGGC TCCGCGCCTG TCCTTTCTCC CCGCGGGACA
     3061 CACGCGCAGA CTGTCGACGG CCCCCCCGAC CGATGTCAGC CTGGGGGACG AGCTCCACTT
     3121 AGACGGCGAG GACGTGGCGA TGGCGCATGC CGACGCGCTA GACGATTTCG ATCTGGACAT
     3181 GTTGGGGGAC GGGGATTCCC CGGGTCCGGG ATTTACCCCC CACGACTCCG CCCCCTACGG
     3241 CGCTCTGGAT ATGGCCGACT TCGAGTTTGA GCAGATGTTT ACCGATGCCC TTGGAATTGA
     3301 CGAGTACGGT GGGAGCGGCC GCGAGGGCAG AGGAAGTCTG CTAACATGCG GTGACGTCGA
     3361 GGAGAATCCT GGCCCAATGC CCAAGAAGAA GAGGAAGGGA GGTGGTGGAA GCAAGCTACT
     3421 GTCTTCTATC GAACAAGCAT GCGATATTTG CCGACTTAAA AAGCTCAAGT GCTCCAAAGA
     3481 AAAACCGAAG TGCGCCAAGT GTCTGAAGAA CAACTGGGAG TGTCGCTACT CTCCCAAAAC
     3541 CAAAAGGTCT CCGCTGACTA GGGCACATCT GACAGAAGTG GAATCAAGGC TAGAAAGACT
     3601 GGAACAGCTA TTTCTACTGA TTTTTCCTCG AGAAGACCTT GACATGATTT TGAAAATGGA
     3661 TTCTTTACAG GATATAAAAG CATTGTTAAC AGGATTATTT GTACAAGATA ATGTGAATAA
     3721 AGATGCCGTC ACAGATAGAT TGGCTTCAGT GGAGACTGAT ATGCCTCTAA CATTGAGACA
     3781 GCATAGAATA AGTGCGACAT CATCATCGGA AGAGAGTAGT AACAAAGGTC AAAGACAGTT
     3841 GACTGTATCG CCGGAATTCG GCAAGAACAT GCAGGCGGTC ACCGAGCTGC ATTCCCGGCT
     3901 GATCGCTGCG CAGCAGGCGA TGGAGCGCGA CTATTGGCGG TTGCGTGAAT TGGAGACTCG
     3961 CTACCGCCTG GTGTTCGACG CTGCCGCCGA TGCGGTGATG ATCGTCTCCG CCGGCGACAT
     4021 GCGCATCGTC GAAGCCAACC GGGCGGCGGT GAATGCGATC AGCCGCGTCG AGCGCGGCAA
     4081 TGACGACCTT GCGGGGCGTG ATTTCCTCGC CGAAGTGGCG GCTGCCGATC GCGATGCGGT
     4141 GCGCGACATG CTGGCCCAGG TGCGTCAGCG CGGCACCGCA CTCAGCGTCC TCGTTCATCT
     4201 CGGCCGTTAC GACCGCGCCT GGATGCTGCG CGGTTCGCTG ATGTCGTCCG AGCGTCGTCA
     4261 GGTTTTCCTG CTGCACTTCA CCCCGGTGAC CACGACTCCC GCGATCGACG ACGACGACAA
     4321 AGGTGTCGTT GCTTCTGCTG CCGACGGTGC AGAGGGCGCC TCAGACGACG CAGAGGATTA
     4381 AAGCTGCAAT AAACAAGTTA ACAACAAACT AGTACCTAGG TAACTACAAG GAACCCCTAG
     4441 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA
     4501 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCCAGC
     4561 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT
     4621 GGCGAATGGA ATTCCAGACG ATTGAGCGTC AAAATGTAGG TATTTCCATG AGCGTTTTTC
     4681 CTGTTGCAAT GGCTGGCGGT AATATTGTTC TGGATATTAC CAGCAAGGCC GATAGTTTGA
     4741 GTTCTTCTAC TCAGGCAAGT GATGTTATTA CTAATCAAAG AAGTATTGCG ACAACGGTTA
     4801 ATTTGCGTGA TGGACAGACT CTTTTACTCG GTGGCCTCAC TGATTATAAA AACACTTCTC
     4861 AGGATTCTGG CGTACCGTTC CTGTCTAAAA TCCCTTTAAT CGGCCTCCTG TTTAGCTCCC
     4921 GCTCTGATTC TAACGAGGAA AGCACGTTAT ACGTGCTCGT CAAAGCAACC ATAGTACGCG
     4981 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
     5041 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
     5101 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
     5161 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
     5221 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC
     5281 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG
     5341 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG
     5401 AATTTTAACA AAATATTAAC GTTTACAATT TAAATATTTG CTTATACAAT CTTCCTGTTT
     5461 TTGGGGCTTT TCTGATTATC AACCGGGGTA CATATGATTG ACATGCTAGT TTTACGATTA
     5521 CCGTTCATCG ATTCTCTTGT TTGCTCCAGA CTCTCAGGCA ATGACCTGAT AGCCTTTGTA
     5581 GAGACCTCTC AAAAATAGCT ACCCTCTCCG GCATGAATTT ATCAGCTAGA ACGGTTGAAT
     5641 ATCATATTGA TGGTGATTTG ACTGTCTCCG GCCTTTCTCA CCCGTTTGAA TCTTTACCTA
     5701 CACATTACTC AGGCATTGCA TTTAAAATAT ATGAGGGTTC TAAAAATTTT TATCCTTGCG
     5761 TTGAAATAAA GGCTTCTCCC GCAAAAGTAT TACAGGGTCA TAATGTTTTT GGTACAACCG
     5821 ATTTAGCTTT ATGCTCTGAG GCTTTATTGC TTAATTTTGC TAATTCTTTG CCTTGCCTGT
     5881 ATGATTTATT GGATGTTGGA ATTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT
     5941 ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC
     6001 CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA
     6061 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG
     6121 TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT
     6181 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA
     6241 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA
     6301 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT
     6361 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG
     6421 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG
     6481 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG
     6541 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG
     6601 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA
     6661 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG
     6721 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC
     6781 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG
     6841 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG
     6901 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC
     6961 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG
     7021 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG
     7081 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT
     7141 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA
     7201 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT
     7261 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG
     7321 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT
     7381 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT
     7441 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
     7501 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT
     7561 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC
     7621 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG
     7681 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
     7741 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
     7801 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG
     7861 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA
     7921 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT
     7981 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT
     8041 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA
     8101 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG
     8161 CCTCTCCCCG CGCGTTGGCC GATT
//
