LOCUS       pZH509_GFPmut2          4215 bp ds-DNA     circular     26-SEP-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     GFPmut2 polycystronic expression w/ TetR from pLTet promoter;
            inducible w/ aTc or other tetracycline analogs.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        189..262
                     /locus_tag="pLtetO-1"
                     /ApEinfo_label="pLtetO-1"
                     /ApEinfo_fwdcolor="#18ff11"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            3137..4215
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    189..207
                     /locus_tag="TetO2"
                     /ApEinfo_label="TetO2"
                     /ApEinfo_fwdcolor="#ff120b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    214..232
                     /locus_tag="TetO2(1)"
                     /ApEinfo_label="TetO2"
                     /ApEinfo_fwdcolor="#ff120b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1703..1746
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRPAANDENYAASV*"
                     /locus_tag="rrnB_T1_terminator"
                     /ApEinfo_label="rrnB_T1_terminator"
                     /ApEinfo_fwdcolor="#b1a0ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             1018..1023
                     /locus_tag="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2152..2995
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /locus_tag="p15A_origin"
                     /ApEinfo_label="p15A_origin"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      208..213
                     /locus_tag="-33"
                     /ApEinfo_label="-33"
                     /ApEinfo_fwdcolor="#ff4669"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      231..236
                     /locus_tag="-10"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="#ff5d86"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..243
                     /locus_tag="pLtetO-1 +1"
                     /ApEinfo_label="pLtetO-1 +1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1032..1655
                     /locus_tag="TetR tn10"
                     /ApEinfo_label="TetR tn10"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             292..1005
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
                     /locus_tag="GFPmut2"
                     /ApEinfo_label="GFPmut2"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
      241 ACATCAGCAG GACGCACTGA CCgaattcat taaagaggag aaaggtaccg catgagtaaa
      301 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      361 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      421 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      481 ttcgcgtatg gtcttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      541 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      601 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      661 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      721 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      781 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      841 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
      901 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      961 gtaacagctg ctgggattac acatggcatg gatgaactat acaaaTAAta atctagcagg
     1021 aggatttcac cATGTCTAGA TTAGATAAAA GTAAAGTGAT TAACAGCGCA TTAGAGCTGC
     1081 TTAATGAGGT CGGAATCGAA GGTTTAACAA CCCGTAAACT CGCCCAGAAG CTAGGTGTAG
     1141 AGCAGCCTAC ATTGTATTGG CATGTAAAAA ATAAGCGGGC TTTGCTCGAC GCCTTAGCCA
     1201 TTGAGATGTT AGATAGGCAC CATACTCACT TTTGCCCTTT AGAAGGGGAA AGCTGGCAAG
     1261 ATTTTTTACG TAATAACGCT AAAAGTTTTA GATGTGCTTT ACTAAGTCAT CGCGATGGAG
     1321 CAAAAGTACA TTTAGGTACA CGGCCTACAG AAAAACAGTA TGAAACTCTC GAAAATCAAT
     1381 TAGCCTTTTT ATGCCAACAA GGTTTTTCAC TAGAGAATGC ATTATATGCA CTCAGCGCTG
     1441 TGGGGCATTT TACTTTAGGT TGCGTATTGG AAGATCAAGA GCATCAAGTC GCTAAAGAAG
     1501 AAAGGGAAAC ACCTACTACT GATAGTATGC CGCCATTATT ACGACAAGCT ATCGAATTAT
     1561 TTGATCACCA AGGTGCAGAG CCAGCCTTCT TATTCGGCCT TGAATTGATC ATCTGCGGAT
     1621 TAGAAAAACA ACTTAAATGT GAAAGTGGGT CTTAATAAct gcagcccggg ggatcccatg
     1681 gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1741 ttttatAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT
     1801 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG
     1861 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT
     1921 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
     1981 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
     2041 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
     2101 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG Cgcgctagcg
     2161 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     2221 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     2281 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     2341 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     2401 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     2461 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2521 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     2581 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     2641 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     2701 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     2761 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     2821 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     2881 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     2941 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atattGATCT
     3001 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     3061 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     3121 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     3181 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     3241 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
     3301 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     3361 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     3421 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     3481 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     3541 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     3601 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     3661 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     3721 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     3781 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     3841 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     3901 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     3961 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     4021 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     4081 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     4141 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
     4201 CGAGGCCCTT TCGTC    
//
