LOCUS       pZH519_autoreg_T        4333 bp ds-DNA     circular     26-SEP-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pZH519 -- modified pZH512 to have autoregulated TetR expression
            w/out bicistronic expression and moderate GFPmut2 RBS strength
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        189..262
                     /locus_tag="pLtetO-1"
                     /ApEinfo_label="pLtetO-1"
                     /ApEinfo_fwdcolor="#18ff11"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1062..1135
                     /locus_tag="pLtetO-1(1)"
                     /ApEinfo_label="pLtetO-1"
                     /ApEinfo_fwdcolor="#18ff11"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            3255..4333
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    189..207
                     /locus_tag="TetO2"
                     /ApEinfo_label="TetO2"
                     /ApEinfo_fwdcolor="#ff120b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    1062..1080
                     /locus_tag="TetO2(1)"
                     /ApEinfo_label="TetO2"
                     /ApEinfo_fwdcolor="#ff120b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    214..232
                     /locus_tag="TetO2(2)"
                     /ApEinfo_label="TetO2"
                     /ApEinfo_fwdcolor="#ff120b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    1087..1105
                     /locus_tag="TetO2(3)"
                     /ApEinfo_label="TetO2"
                     /ApEinfo_fwdcolor="#ff120b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1821..1864
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRPAANDENYAASV*"
                     /locus_tag="rrnB_T1_terminator"
                     /ApEinfo_label="rrnB_T1_terminator"
                     /ApEinfo_fwdcolor="#b1a0ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      1018..1061
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRPAANDENYAASV*"
                     /locus_tag="rrnB_T1_terminator(1)"
                     /ApEinfo_label="rrnB_T1_terminator"
                     /ApEinfo_fwdcolor="#b1a0ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             1136..1141
                     /locus_tag="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2270..3113
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /locus_tag="p15A_origin"
                     /ApEinfo_label="p15A_origin"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      208..213
                     /locus_tag="-33"
                     /ApEinfo_label="-33"
                     /ApEinfo_fwdcolor="#ff4669"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      1081..1086
                     /locus_tag="-33(1)"
                     /ApEinfo_label="-33"
                     /ApEinfo_fwdcolor="#ff4669"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      231..236
                     /locus_tag="-10"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="#ff5d86"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      1104..1109
                     /locus_tag="-10(1)"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="#ff5d86"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..243
                     /locus_tag="pLtetO-1 +1"
                     /ApEinfo_label="pLtetO-1 +1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1116..1116
                     /locus_tag="pLtetO-1 +1(1)"
                     /ApEinfo_label="pLtetO-1 +1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1150..1773
                     /locus_tag="TetR tn10"
                     /ApEinfo_label="TetR tn10"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             292..1005
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
                     /locus_tag="GFPmut2"
                     /ApEinfo_label="GFPmut2"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
      241 ACATCAGCAG GACGCACTGA CCgaattcat taaagCCgag aaaggtaccg catgagtaaa
      301 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      361 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      421 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      481 ttcgcgtatg gtcttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      541 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      601 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      661 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      721 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      781 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      841 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
      901 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      961 gtaacagctg ctgggattac acatggcatg gatgaactat acaaaTAAta atctagcata
     1021 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tTCCCTATCA GTGATAGAGA
     1081 TTGACATCCC TATCAGTGAT AGAGATACTG AGCACATCAG CAGGACGCAC TGACCaggag
     1141 gatttcaccA TGTCTAGATT AGATAAAAGT AAAGTGATTA ACAGCGCATT AGAGCTGCTT
     1201 AATGAGGTCG GAATCGAAGG TTTAACAACC CGTAAACTCG CCCAGAAGCT AGGTGTAGAG
     1261 CAGCCTACAT TGTATTGGCA TGTAAAAAAT AAGCGGGCTT TGCTCGACGC CTTAGCCATT
     1321 GAGATGTTAG ATAGGCACCA TACTCACTTT TGCCCTTTAG AAGGGGAAAG CTGGCAAGAT
     1381 TTTTTACGTA ATAACGCTAA AAGTTTTAGA TGTGCTTTAC TAAGTCATCG CGATGGAGCA
     1441 AAAGTACATT TAGGTACACG GCCTACAGAA AAACAGTATG AAACTCTCGA AAATCAATTA
     1501 GCCTTTTTAT GCCAACAAGG TTTTTCACTA GAGAATGCAT TATATGCACT CAGCGCTGTG
     1561 GGGCATTTTA CTTTAGGTTG CGTATTGGAA GATCAAGAGC ATCAAGTCGC TAAAGAAGAA
     1621 AGGGAAACAC CTACTACTGA TAGTATGCCG CCATTATTAC GACAAGCTAT CGAATTATTT
     1681 GATCACCAAG GTGCAGAGCC AGCCTTCTTA TTCGGCCTTG AATTGATCAT CTGCGGATTA
     1741 GAAAAACAAC TTAAATGTGA AAGTGGGTCT TAATAActgc agcccggggg atcccatggt
     1801 acgcgtgcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1861 ttatAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA
     1921 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG
     1981 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
     2041 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
     2101 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
     2161 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
     2221 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCg cgctagcgga
     2281 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     2341 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     2401 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     2461 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     2521 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     2581 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2641 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     2701 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     2761 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     2821 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     2881 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     2941 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     3001 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     3061 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat attGATCTTT
     3121 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     3181 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     3241 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     3301 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     3361 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA
     3421 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     3481 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     3541 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     3601 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     3661 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     3721 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     3781 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     3841 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3901 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3961 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     4021 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     4081 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     4141 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     4201 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
     4261 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
     4321 AGGCCCTTTC GTC    
//
