LOCUS       Exported               11130 bp ds-DNA     circular SYN 13-JUN-2017
DEFINITION  pMZ374 sequence 11384 bps.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2017 from SnapGene 4.0.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      75..322
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(338..358)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(371..4474)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(4495..5236)
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     promoter        5265..5369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5370..6230
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6401..6989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7277..7298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7313..7343
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7351..7367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7375..7391
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7428..7784
                     /label=rrk1 gene -1 to -358
     promoter        7428..7784
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     misc_feature    7765..7784
                     /label=20bp
     misc_feature    7785..7792
                     /label=Not1 Cutting Site
     misc_feature    7793..7830
                     /label=sgRNA Cassette
     misc_feature    7793..7812
                     /label=20bp
     misc_RNA        7877..7928
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     7964..8019
                     /label=YZ826
     primer_bind     complement(7978..7994)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(7995..9701)
                     /label=my design
     misc_feature    complement(7995..9477)
                     /label=his3 region in pBG1
     misc_feature    complement(8063..9501)
                     /label=his3 transcipt
     CDS             complement(8238..9392)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his3"
                     /product="imidazole acetol phosphate transaminase, required
                     for histidine biosynthesis"
                     /label=S. pombe his3
                     /translation="MFDLNTCLRKNILELQPYRCARDDFSEGVLLDANECAYGSVISVD
                     GVEFNRYPDPRQIEVKQRLCDLRNKELSITKPLTPDNICMGVGSDEIIDSLIRISCIPG
                     KDKILMCPPSYGMYTVSAKINDVEVVKVLLEPDFNLNVDAICETLSKDSAIKVFFACSP
                     GNPTAKALKLEDIKKILEHPTWNGIVVVDEAYIDFSAPDMSALTLVNEYPNLAVCQTLS
                     KSFGLAGIRIGFCLTSKPIATIMNSLKAPYNISEPTSRLALDALSPQSIDKMHTYRDAI
                     IQQRVRLCKELTTIKGMGKIIGGYDANFILIQVLDRPEGGKPSNDAAKYLYLQMATMHK
                     VVVRFRGTEPLCEGALRITVGTEEENTILLKTIKLVLQEYYTKK"
     misc_feature    complement(8673..8732)
                     /label=YZ492
     misc_feature    8673..8732
                     /label=YZ493
     misc_feature    8701..8705
                     /label=one intron deleted here
     misc_feature    8706
                     /label=T to A here to remove EcoRI
     misc_feature    8847..8906
                     /label=YZ494
     misc_feature    complement(8847..8906)
                     /label=YZ495
     misc_feature    8874
                     /label=G to C to remove XhoI
     misc_feature    complement(9003..9062)
                     /label=YZ497
     misc_feature    9003..9062
                     /label=YZ496
     misc_feature    9033
                     /label=G to C here to move BsaI
     misc_feature    9210..9269
                     /label=YZ498
     misc_feature    complement(9210..9269)
                     /label=YZ499
     misc_feature    9234
                     /label=C to g here to remove BamHI
     misc_feature    9290..9347
                     /label=YZ500
     misc_feature    complement(9290..9347)
                     /label=YZ501
     misc_feature    complement(9393..9501)
                     /label=5' UTR
     misc_feature    9484..9492
                     /label=possible TATA box
     misc_feature    9564..9623
                     /label=YZ502
     misc_feature    complement(9564..9623)
                     /label=YZ503
     misc_feature    9593
                     /label=C to g here to remove NdeI
     primer_bind     complement(9675..9731)
                     /label=YZ827
     misc_feature    complement(9702..9731)
                     /label=downstream
     rep_origin      9811..10592
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacggg gatcgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt
      121 ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat
      181 ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca
      241 ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag
      301 cgtgacataa ctaattacat gactcgagaa gagatcacac cttcctcttc ttcttggggt
      361 cagccctgct gtctccaccg agctgagaga ggtcgattct tgtttcatag agccccgtaa
      421 ttgactgatg aatcagtgtg gcgtccagga cctcctttgt agaggtgtac cgctttctgt
      481 ctatggtggt gtcgaagtac ttgaaggctg caggcgcgcc caagttggtc agagtaaaca
      541 agtggataat gttttctgcc tgctccctga tgggcttatc cctgtgctta ttgtaagcag
      601 aaagcacctt atcgaggtta gcgtcggcga ggatcactct tttggagaat tcgcttattt
      661 gctcgatgat ctcatcaagg tagtgtttgt gttgttccac gaacagctgc ttctgctcat
      721 tatcttcggg agaccctttg agcttttcat agtggctggc cagatacaag aaattaacgt
      781 atttagaggg cagtgccagc tcgttacctt tctgcagctc gcccgcacta gcgagcattc
      841 gtttccggcc gttttcaagc tcaaagagag agtacttggg aagcttaatg atgaggtctt
      901 ttttgacctc tttatatcct ttcgcctcga gaaagtcgat ggggtttttt tcgaagcttg
      961 atcgctccat gattgtgatg cccagcagtt ccttgacgct tttgagtttt ttagacttcc
     1021 ctttctccac tttggccaca accagtacac tgtaagcgac tgtaggagaa tcgaatccgc
     1081 cgtatttctt ggggtcccaa tcttttttgc gtgcgatcag cttgtcgctg ttccttttcg
     1141 ggaggatact ttccttggag aagcctccgg tctgtacttc ggtcttttta acgatgttca
     1201 cctgcggcat ggacaggacc ttccggactg tcgcgaaatc cctacccttg tcccacacga
     1261 tttctcctgt ttctccgttt gtttcgataa gtggtcgctt ccgaatctct ccattggcca
     1321 gtgtaatctc ggtcttgaaa aaattcataa tattgctgta aaagaagtac ttagcggtgg
     1381 ccttgcctat ttcctgctca gactttgcga tcattttcct aacatcgtac actttatagt
     1441 ctccgtaaac aaattcagat tcaagcttgg gatatttttt gataagtgca gtgcctacca
     1501 ctgcattcag gtaggcatca tgcgcatggt ggtaattgtt gatctctctc accttataaa
     1561 actgaaagtc ctttctgaaa tctgagacca gcttagactt cagagtaata actttcacct
     1621 ctcgaatcag tttgtcattt tcatcgtact tggtgttcat gcgtgaatcg agaatttggg
     1681 ccacgtgctt ggtgatctgg cgtgtctcaa caagctgcct tttgatgaag ccggctttat
     1741 ccaactcaga caggccacct cgttcagcct tagtcagatt atcgaacttc cgttgtgtga
     1801 tcagtttggc gttcagcagc tgccgccaat aatttttcat tttcttgaca acttcttctg
     1861 aggggacgtt atcactcttc cctctatttt tatcggatct tgtcaacact ttattatcaa
     1921 tagaatcatc tttgagaaaa gactggggca cgatatgatc cacgtcgtag tcggagagcc
     1981 gattgatgtc cagttcctga tccacgtaca tgtccctgcc gttctgcagg tagtacaggt
     2041 agagcttctc attctgaagc tgggtgtttt caactgggtg ttccttaagg atttgggacc
     2101 ccagttcttt tataccctct tcaatcctct tcatcctttc cctactgttc ttctgtccct
     2161 tctgggtagt ttggttctct cgggccatct cgataacgat attctcgggc ttatgccttc
     2221 ccattacttt gacgagttca tccacgacct taacggtctg cagtattccc tttttgatag
     2281 ctgggctacc tgcaagatta gcgatgtgct cgtgaagact gtccccctgg ccagaaactt
     2341 gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc atggatcaac tgcatgaagt
     2401 tccggttggc aaatccatcg gacttaagaa aatccaggat tgtctttcca gactgcttat
     2461 ctcggatccc attgatcagt tttcttgaca gccgccccca tcctgtatat cggcgcctct
     2521 tgagctgttt catgactttg tcgtcgaaga gatgagcgta agttttcaag cgttcttcaa
     2581 tcatctccct atcttcaaac aacgtaaggg tgaggacaat gtcctcaaga atgtcctcgt
     2641 tctcctcatt gtccaggaag tccttgtctt taatgatttt caggagatcg tgatacgttc
     2701 ccagggatgc gttgaagcga tcctccactc cgctgatttc aacagagtcg aaacattcaa
     2761 tctttttgaa atagtcttct ttgagctgtt tcacggtaac tttccggttc gtcttgaaga
     2821 ggaggtccac gatagctttc ttctgctctc cagacaggaa tgctggcttt ctcatccctt
     2881 ctgtgacgta tttgaccttg gtgagctcgt tataaactgt gaagtactcg tacagcagag
     2941 agtgtttagg aagcaccttt tcgttaggca gatttttatc aaagttagtc atcctttcga
     3001 tgaaggactg ggcagaggcc cccttatcca cgacttcctc gaagttccag ggagtgatgg
     3061 tctcttctga tttgcgagtc atccacgcga atctggaatt tccccgggcg agggggccta
     3121 catagtaggg tatccgaaat gtgaggattt tctcaatctt ttccctgtta tctttcaaaa
     3181 aggggtagaa atcctcttgc cgcctgagga tagcgtgcag ttcgcccagg tgaatctggt
     3241 gggggatgct tccattgtcg aaagtgcgct gtttgcgcaa cagatcttct ctgttaagct
     3301 ttaccagcag ctcctcggtg ccgtccattt tttccaagat gggcttaata aatttgtaaa
     3361 attcctcctg gcttgctccg ccgtcaatgt atccggcgta gccattttta gactgatcga
     3421 agaaaatttc cttgtacttc tcaggcagtt gctgtctgac aagggccttc agcaaagtca
     3481 agtcttggtg gtgctcatca tagcgcttga tcatactagc gctcagcgga gctttggtga
     3541 tctccgtgtt cactcgcaga atatcactca gcagaatggc gtctgacagg ttctttgccg
     3601 ccaaaaaaag gtctgcgtac tggtcgccga tctgggccag cagattgtcg agatcatcat
     3661 cgtaggtgtc tttgctcagt tgaagcttgg catcttcggc caggtcgaag ttagatttaa
     3721 agttgggggt cagcccgagt gacagggcga taagattacc aaacaggccg ttcttcttct
     3781 ccccagggag ctgtgcgatg aggttttcga gccgccggga tttggacagc ctagcgctca
     3841 ggattgcttt ggcgtcaact ccggatgcgt tgatcgggtt ctcttcgaaa agctgattgt
     3901 aagtctgaac cagttggata aagagtttgt cgacatcgct gttgtctggg ttcaggtccc
     3961 cctcgatgag gaagtgtccc cgaaatttga tcatatgcgc cagcgcgaga tagatcaacc
     4021 gcaagtcagc cttatcagta ctgtctacaa gcttcttcct cagatgatat atggttgggt
     4081 acttttcatg gtacgccacc tcgtccacga tattgccaaa gattgggtgg cgctcgtgct
     4141 ttttatcctc ctccaccaaa aaggactcct ccagcctatg gaagaaagag tcatccacct
     4201 tagccatctc attactaaag atctcctgca ggtagcagat ccgattcttt ctgcgggtat
     4261 atctgcgccg tgctgttctt ttgagccgcg tggcttcggc cgtctccccg gagtcgaaca
     4321 ggagggcgcc aatgaggttc ttctttatgc tgtggcgatc ggtattgccc agaactttga
     4381 attttttgct cggcaccttg tactcgtccg taatgacggc ccaaccgacg ctgtttgtgc
     4441 cgatatcgag cccaatggag tacttcttgt ccatctctag agtcgacctg cagcaattct
     4501 cttgcttaaa gaaaagcgaa ggcacctgtc caccacccac tatttatacc atgggaggcg
     4561 tcaaaccgag tttttgcgaa aggattatcg cacctctccc caagtaacat cggcaaagca
     4621 aaaacggagc ggaagaccaa tgagacgcgg aaggggagcg ccgaatgagg aaagcgagag
     4681 agaaagaagt ggatggaatg attccccgcc aaagtggaca catttcggga atcttcagga
     4741 agcatggatc accttatgga caagaagata atccggaagc caataggagg gcgacagggg
     4801 gtcagcgata agcgtcggaa aaaactttat gatatggtaa atacgtagga ggttacggtg
     4861 cagaggaggg gccggtagcg agtgatagcg agggaaagac gatcgctatc gggccgtaag
     4921 gaggaaatgg gaggttgggg gaaggatgcg ttggaatgcg gagtagagaa attgaagaga
     4981 gagagaggac acacctagag agaaagaaat ggatatagag aaagagaaaa aataagagga
     5041 atacggatac gatggagtgc gttgaatgag agaaaagaaa agaaaaggaa tgataagaga
     5101 aggaatgaaa attgttttgt tttgttttca attgctttct tttgttttct ttgtctttgt
     5161 tcttccttcc cttgtcccga tgctgttctt ctcaccagct tccgcatgat agccattttc
     5221 ttagttgttg tagggcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5281 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5341 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5401 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5461 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5521 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5581 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5641 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5701 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5761 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5821 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5881 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5941 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6001 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6061 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6121 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6181 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6241 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7141 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7201 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7261 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7321 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7381 acagctatga catgattacg aattcgagct cggtaccccg ggtacctttt gcttatgttg
     7441 gtggtagttg gcatgcgtag actgatgact agtcagcaag gagcgtagaa cagtcacact
     7501 cgttatatat gtgcttccaa gaaaactcaa gaatttacca ttagcaaaca cttttttgaa
     7561 atgttagaca tttaaatgac gaaggcatat agaagctttg aataggtgtt gtaaagtgtt
     7621 gatttatgtg acgctgaggg tgcgcatgaa aggaatgttg ggtcacgatt attaaacagt
     7681 ttgctagctt ggacacttga gtattggaag ttgttgaatt ctaaaaaact ttcagttgat
     7741 ttgaatagtt gctgttgcca aaaaacataa cctgtaccga agaagcggcc gcgttttaga
     7801 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa agtgagtggc
     7861 accgagtcgg tggtgccctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac
     7921 gaaacagggg taccgtgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcact
     7981 ggccgtcgtt ttacaaatta gcgtaactcc tttacaaaat taaaaataac atcttttcat
     8041 tgcttatcga actatacgga aacacaccaa aaaggaagaa aacgtatttt aataaagaaa
     8101 gaaggaatgc aagcgcaatt agcgaacaca aaatatattg ggtacatcag aaaagaaatg
     8161 tgacgagtcc ggaaggggaa aggaaagagt tataatttgt ctaattgaaa cgttctaata
     8221 ggccagaaaa ggaaacctca tttttttgta tagtattcct gcaatacaag tttaatagtt
     8281 tttagtaata tcgtattctc ttcttctgtg ccaacagtga tacgcaatgc tccttcgcaa
     8341 agaggctctg tacctctaaa tctaacaacg actttgtgca tcgtggccat ttgtaaataa
     8401 agatattttg cggcatcgtt actaggctta cctccttctg gtctatctaa aacctgaatg
     8461 aggataaaat ttgcatcata accacctata atcttgccca ttcctttaat ggtagtaagc
     8521 tctttgcaaa gacgaactct ttgctgtata atagcatctc tatatgtatg cattttatca
     8581 atcgactgag gggataaagc atccaaagct aaacgagagg tgggttcgct aatgttataa
     8641 ggagccttta atgagttcat aatggtagca ataggtttac ttgtcaaaca aaatccgata
     8701 cgaatacctg ctagaccgaa agatttcgaa agggtttggc aaacagcaag gtttggatat
     8761 tcattgacaa gagttaaggc agacatatcg ggagcagaga aatcaatata ggcctcatcg
     8821 acgacaacga ttccattcca tgtagggtgc tccaaaatct tcttaatatc ctccagttta
     8881 agagccttag ctgttggatt accaggggaa caagcaaaaa agactttgat ggcactatct
     8941 ttagaaagtg tttcgcaaat agcatcgacg ttcaaattga aatctggttc aaggagaacc
     9001 ttaacaacct caacatcgtt gatttttgca gacaccgtat acattccgta cgaaggtgga
     9061 cacatcaaaa tcttatcttt tccaggaata caagaaatgc gaattaagga gtcaataatt
     9121 tcatcgctgc caacacccat gcaaatgtta tctggtgtta gtggcttggt aatggaaagc
     9181 tctttgtttc tcaaatcaca taatctttgc tttacttcga tctgtctagg atcgggataa
     9241 cgattaaatt ccacaccgtc cacggaaata accgaaccat aagcacattc attggcatca
     9301 agaaggacac cttcagagaa gtcatctctc gcacagcgat aaggctgaag ttctaagata
     9361 tttttacgaa gacaagtatt caaatcaaac atcgtatctc tttcgatgtg tttttgttaa
     9421 agtaagaaga aagcgatcga tgagattaat taataattaa cctaagatgc aggtgatatc
     9481 tataaatata tagtgtgtag caatgtgagt ctttaattca ttacaacgtg tatcaccgtg
     9541 tcattatatc caataaattc agaaacctga agaggaaaga gaaataaagc tcgatatgtc
     9601 tttcatttcc aaatacaatt ataagtgcaa tagtaaagaa aacgttgaaa gcttgataaa
     9661 aatttcgtta ctatactaat gctgtaaaga aaatgcacca ttacatatag ccagtgggat
     9721 ttgtagctaa gctcatcgat ggcgagtcta actccttaac cactcggaca tagtgactta
     9781 tctgacactc attgtaaatt aatatatata taggcatttt gtttagttaa aggtacttaa
     9841 gtaattagta taaacgaacc aattttataa tcaggaagtt aagtgaatgg tagcacatgt
     9901 cgtaaaaatt gtgaattttt attgaataat attttaaata caagcctttc tagactaggt
     9961 atactcataa acatatgagc aaaaggatag aggagattac attgcatctt ctacaaattt
    10021 atttattgcc ctttactgaa aaattaaata atgagtacta aatgataaaa agcgctcagt
    10081 acaagaaaga ttgcaaaaat attgcattct tcatgaatta aagttgcata taaggcatat
    10141 tgaaagtaat agtactaaaa cagcagttag cgaaaattaa tagaattata ttcgcaagac
    10201 aattgtgaca ttaaattaaa aaattgtaga atttttacta tcctctttaa cgccatgagc
    10261 ctttataaaa aggttaaatt agttttaaca ttcttttttt gagtaagagt ttacaattta
    10321 tcaaaacctg tgttattata ttcattagtt tcaatttatt agcatctaga gaaaaatcaa
    10381 ttggcagtta cattgttgga atttatgaag aaaaagaatc tacaacggag aataagttgc
    10441 tgatcgcttt ccctaaaatt gtatattttg ctgagcttat tttgacattt cgttgaagtt
    10501 ttctctaatt cgcattcatt ttaagtaaaa caatgagaaa taaaattaca aaaaatacaa
    10561 attaaaatac aatttttagc tataattata gacgatgccc ttgtatccca ttctgtctcg
    10621 cttgccccta ctttttatct tttatatacc ataatgaacg ctgccgctac taaccatacc
    10681 ccgattttac atttcggact cccaaggacg tacaaaatag aaaactatag aaaaaaataa
    10741 tcagaaaata gcatgtcatc tctttgtaaa acgcgtttgc aagaagaaag gaaacaatgg
    10801 agaagagatc atccatttgt atgtaaattt tagtaaactt gaagaaatca ctaacaactt
    10861 ctcttactta gggattctat gcaaaacctt gtaaatcatc tgatggagga ctcgatttaa
    10921 tgaattggaa ggttggccat cgattatggt gcactctcag tacaatctgc tctgatgccg
    10981 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    11041 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    11101 ggttttcacc gtcatcaccg aaacgcgcga
//
