LOCUS       hisSUMO-ZFP57-fu        3510 bp ds-DNA     circular SYN 12-JAN-2017
DEFINITION  Sequence verified.
ACCESSION
VERSION     .
KEYWORDS    Untitled.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Zheng and Zuo
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 13, 2016 from SnapGene Viewer 3.0.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        26..44
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             127..417
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /note="SUMO"
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTP
                     LRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
     CDS             418..435
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /note="thrombin site"
                     /translation="LVPRGS"
     CDS             436..1347
                     /codon_start=1
                     /note="eZFP57-full"
                     /translation="TRVLQASQAGPPFFCYTCGKCFSRRSYLYSHQFVHNPKLTNSCS
                     QCGKLFRSPKSLSYHRRMHLGERPFCCTLCDKTYCDASGLSRHRRVHLGYRPHSCSVC
                     GKSFRDQSELKRHQKIHQNQEPVDGNQECTLRIPGTQAEFQTPIARSQRSIQGLLDVN
                     HAPVARSQEPIFRTEGPMAQNQASVLKNQAPVTRTQAPITGTLCQDARSNSHPVKPSR
                     LNVFCCPHCSLTFSKKSYLSRHQKAHLTEPPNYCFHCSKSFSSFSRLVRHQQTHWKQK
                     SYLCPICDLSFGEKEGLMDHWRGYKGKG"
     terminator      1502..1549
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1767..2355)
     CDS             complement(2527..3387)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(3388..3492)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN      
        1 gctagtggtg ctagccccgc gaaattaata cgactcacta tagggtctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg catcaccatc accatcacgg ttcttctatg
      121 gctagcatgt cggactcaga agtcaatcaa gaagctaagc cagaggtcaa gccagaagtc
      181 aagcctgaga ctcacatcaa tttaaaggtg tccgatggat cttcagagat cttcttcaag
      241 atcaaaaaga ccactccttt aagaaggctg atggaagcgt tcgctaaaag acagggtaag
      301 gaaatggact ccttaagatt cttgtacgac ggtattagaa ttcaagctga tcagacccct
      361 gaagatttgg acatggagga taacgatatt attgaggctc acagagaaca gattggtctg
      421 gtgccacgcg gtagtacccg tgtgttgcaa gcgtcacaag ctggcccacc cttcttttgc
      481 tatacgtgcg gcaagtgttt cagtcgtcgt agctatttgt actcccacca gtttgttcac
      541 aacccgaaat tgaccaactc atgcagccag tgcggcaagc tgttccgttc tccgaaaagt
      601 ttaagttacc atcgccgcat gcatttgggt gaacgtccgt tttgctgcac actgtgtgat
      661 aaaacgtact gtgatgcctc cggcctcagt cgccaccgcc gcgtgcacct gggttatcgc
      721 ccacactctt gctctgtttg tggaaaatcc ttccgtgacc aatccgaact gaaacgccat
      781 cagaaaattc atcaaaatca ggaaccggtt gatggaaatc aggaatgcac ccttcgtatt
      841 ccggggacgc aggcggagtt tcaaacgccg attgcacgtt cgcaacgcag cattcagggc
      901 ctgctggatg ttaaccatgc cccagtcgcg cgttcgcagg aaccgatttt ccgcacagag
      961 ggcccgatgg cccagaacca ggcctccgtc ctgaaaaacc aggcgccggt tacgcgtacc
     1021 caagccccaa tcacaggcac tctgtgtcag gatgcgcgct caaacagcca tccggtgaag
     1081 ccgtctcgct taaacgtgtt ctgttgcccg cactgtagct taaccttcag taaaaaaagt
     1141 tatctgtccc gccatcagaa agcacatctg acggagccgc cgaattattg ttttcattgt
     1201 agtaaaagtt tcagctcgtt ttcgcgtttg gtacgccatc agcagacgca ctggaagcaa
     1261 aaatcctacc tgtgcccgat ctgcgatctg agttttggtg aaaaagaagg cctgatggac
     1321 cattggcgcg gttataaagg aaaaggttaa tgaggatccc gggaattctc gagtaaggtt
     1381 aacctgcagg aggcctttaa ttaaggtggt gcggccgcgc tagcggtccc gggggatcga
     1441 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     1501 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     1561 aactatatcc ggaagcttgg cactggccga ccggggtcga gcactgactc gctgcgctcg
     1621 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1681 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgct aagacacgac
     2101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2401 gaaaactcac agatccggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2461 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2521 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2581 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2641 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2701 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2761 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2821 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2881 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2941 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3001 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3061 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3121 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3181 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3241 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3301 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3361 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3421 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3481 aggggttccg cgcacatttc cccgaaaagt
//
