LOCUS       Lv_EF1a_2xFrb-V5       10549 bp    DNA     circular UNA 03-OCT-2017
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...1a-7qyhpp1
VERSION     urn.local...1a-7qyhpp1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_signal     686..823
     misc_feature    1303..1536
                     /note="Geneious type: regulatory sequence"
     misc_structure  2028..2151
     promoter        2209..3380
                     /note="vntifkey=29"
     misc_feature    3392^3393
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3393..3659
                     /note="Geneious type: CDS"
     misc_feature    3666..3689
                     /note="Geneious type: linker"
     misc_feature    3690..3956
                     /note="Geneious type: CDS"
     misc_feature    3836
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3963..4004
                     /note="Geneious type: tag"
     misc_feature    4005..4028
                     /note="Geneious type: linker"
     misc_feature    4037
                     /note="Geneious type: Editing History Replacement"
     gene            4038..5294
     promoter        5329..5832
                     /note="vntifkey=29"
     CDS             5854..6453
                     /note="Geneious type: cds"
     gene            6473..7048
     rep_origin      complement(8372..9042)
     misc_feature    9187..10047
                     /note="Geneious type: resistance gene"
     terminator      10203..10322
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcgg ccatgtggca tgaaggcctg gaagaggcat
     3421 ctcgtttgta ctttggggaa aggaacgtga aaggcatgtt tgaggtgctg gagcccttgc
     3481 atgctatgat ggaacggggc ccccagactc tgaaggaaac atcctttaat caggcctatg
     3541 gtcgagattt aatggaggcc caagagtggt gcaggaagta catgaaatca gggaatgtca
     3601 aggacctcac ccaagcctgg gacctctatt atcatgtgtt ccgacgaatc tcaaagcagt
     3661 tcggcggcgg aggtagcggc ggtggatcaa tgtggcacga gggattggag gaagctagca
     3721 gactttattt cggtgagcgc aatgtcaagg gaatgttcga ggtcctcgaa cctctccacg
     3781 ccatgatgga gagaggacct caaaccctca aagagactag tttcaaccaa gcttatggca
     3841 gggacctgat ggaagctcag gaatggtgtc gcaaatatat gaagagcggc aacgtgaaag
     3901 atttgacaca ggcttgggat ttgtactacc acgtctttag gaggattagc aaacaattcg
     3961 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgggaggc ggatcaggtg
     4021 gcggtagcgc ggccgcaatg tctgtggcgt tcgcagcccc gaggcagcgg ggcaagggcg
     4081 aaatcacgcc cgccgccatc cagaagatgc tggatgaaaa caaccatctt attcagtgta
     4141 taatggacta tcagaacaaa gggaaggcct cggagtgctc gcagtatcag cagatattgc
     4201 atacaaacct ggtatacctt gctacaatag cagactctaa tcaaaatatg cagtctctct
     4261 taccagcacc gcccacacag actatgccaa tgggtcctgg agggatgagt cagagtggcc
     4321 ctccaccccc tccccgctct cacaacatgc cttcagatgg aatggtgggt gggggccctc
     4381 ctgcaccaca catgcagaac cagatgaacg gccagatgcc tgggcctaac catatgccaa
     4441 tgcagggacc tggacccagt cagctcagca tgacaaacag ctccatgaat atgccttcaa
     4501 gtagccatgg ctccatggga ggttacaacc attctgtgcc gtcatcccag agcatgcccg
     4561 tgcagaacca gatgacaatg agtcaggggc agccaatggg aaactatggt cccagaccaa
     4621 acatgaatat gcaaccaaat caagggccga tgatgcacca gcagcctcct tctcagcagt
     4681 acaatatgcc acctggaggg gcacagcatt accaaggaca gcaggcgccc atggggctga
     4741 tgggccaagt taaccaaggc agtcacatga tgggccagcg acagatgcct ccctacagac
     4801 ctccgcaaca gggcccacca cagcagtact caggccagga agactattat ggggaccaat
     4861 acagtcatgg tggacaaggt cctccagaag gcatgaacca gcaatattac cctgatggtc
     4921 ataatgatta cggttatcag caaccgtcgt atcctgaaca aggctacgat aggccttatg
     4981 aggattcctc acaacattac tacgaaggag gaaactccca gtatggccaa cagcaagacg
     5041 cttaccaggg accacctcca cagcaaggat acccacccca gcagcagcag tacccgggac
     5101 agcagggata cccagggcag cagcagagct atggtccttc gcagggcggt ccaggtcctc
     5161 agtatcctaa ttatcctcag ggtcaaggtc agcagtatgg gggctataga ccaacacagc
     5221 caggaccacc ccagccaccc cagcagaggc cttatgggta cgaccaggga cagtatggaa
     5281 attaccagca gtgagccgcg ccggctctag atcgcgaacg cgtgaattct accgggtagg
     5341 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     5401 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     5461 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     5521 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     5581 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     5641 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     5701 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     5761 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     5821 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     5881 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     5941 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6001 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6061 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6121 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6181 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6241 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6301 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6361 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6421 acctggtgca tgacccgcaa gcccggtgcc tgacggggcg cgtctggaac aatcaacctc
     6481 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6541 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6601 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6661 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6721 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6781 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6841 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6901 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6961 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7021 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     7081 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     7141 tgcctggcta gaagcacaag aggaggagga ggtgggtttt tccagtcaca cctcaggtac
     7201 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga
     7261 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7321 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     7381 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     7441 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     7501 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     7561 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     7621 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     7681 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     7741 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7801 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7861 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7921 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     7981 tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     8041 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8101 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8161 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8941 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     9001 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9121 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9181 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9241 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9301 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9361 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9421 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9481 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9541 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9601 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9661 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9721 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9781 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9841 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9901 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9961 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10021 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10081 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10141 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
    10201 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10261 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10321 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10381 cataccctat taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc
    10441 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10501 gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
