LOCUS       616-U6-hGRIN2B-C       10526 bp ds-DNA     linear       23-SEP-2017
DEFINITION  .
ACCESSION   urn.local...83bvw2l-l
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     >pSpCas9n(BB)-2A-Puro ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..250
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     promoter        251..499
                     /created_by="User"
                     /label="U6 promoter"
     sgRNA           501..520
                     /created_by="User"
                     /label="GRIN2B sgRNA"
     misc_feature    500^501
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     RNA             521..560
                     /created_by="User"
                     /modified_by="User"
                     /label="Cas9 binding hairpin"
     RNA             561..613
                     /created_by="User"
                     /modified_by="User"
                     /label="S.pyogenes terminator"
     misc_feature    613^614
                     /Original_Bases="CTCGAGTACTAGGATCCATTAGGCGGCCGCGTGGATAACCG
                     TATTACCGCCATGCAT"
                     /label="CTCGAGTACTAGGATCCATTAGGCGGCCGCGTGGATAACCGTATTACCGC
                     CATGCAT"
                     /note="Geneious type: Editing History Deletion"
     enzyme_site     643..648
                     /created_by="User"
                     /label="Xbal"
     misc_feature    642^643
                     /Original_Bases="TAAGGCTAGTCCGTTTTTAGCGCGTGCGCCAATTCTGCAGA
                     CAAATGGC"
                     /label="TAAGGCTAGTCCGTTTTTAGCGCGTGCGCCAATTCTGCAGACAAATGGC"
                     /note="Geneious type: Editing History Deletion"
     enzyme_site     649..654
                     /created_by="User"
                     /label="KpnI"
     enhancer        655..940
                     /created_by="User"
                     /modified_by="User"
                     /label="CAG enhancer"
                     941..1219
                     /created_by="User"
                     /modified_by="User"
                     /label="chicken beta-actin promoter"
     signal_peptide  1460..1468
                     /created_by="User"
                     /label="kozak"
     tag             1469..1534
                     /created_by="User"
                     /modified_by="User"
                     /label="FLAGx3"
     misc_feature    1468^1469
                     /Original_Bases="TATCCCTACGATGTGCCCGATTACGCTGCGCCCATCACGGC
                     GTACGCCCAGCAGACGAGAGGCCTCCTAGGGTGTATAATCACCAGCCTGACTGGCCGG
                     GACAAAAACCAAGTGGAGGGTGAGGTCCAGATCGTGTCAACTGCTACCCAAACCTTCC
                     TGGCAACGTGCATCAATGGGGTATGCTGGGCAGTCTACCACGGGGCCGGAACGAGGAC
                     CATCGCATCACCCAAGGGTCCTGTCATCCAGATGTATACCAATGTGGACCAAGACCTT
                     GTGGGCTGGCCCGCTCCTCAAGGTTCCCGCTCATTGACACCCTGTACCTGCGGCTCCT
                     CGGACCTTTACCTGGTCACGAGGCACGCCGATGTCATTCCCGTGCGCCGGCGAGGTGA
                     TAGCAGGGGTAGCCTGCTTTCGCCCCGGCCCATTTCCTACTTGAAAGGCTCCTCGGGG
                     GGTCCGCTGTTGTGCCCCGCGGGACACGCCGTGGGCCTATTCAGGGCCGCGGTGTGCA
                     CCCGTGGAGTGGCTAAAGCGGTGGACTTTATCCCTGTGGAGAACCTAGAGACAACCAT
                     GAGATCCCCGGTGTTCACGGACAACTCCTCTCCACCAGCAGTCACCCTGACGCACCCA
                     ATCACCAAAATCGATACCAAATACATCATGACATGCATGTCGGCCGACCTGGAGGTCG
                     TCACGAGCACCTGGGTGCTCGTTGGCGGCGTCCTGGCTGCTCTGGCCGCGTATTGCCT
                     GTCAACAGGCTGCGTGGTCATAGTGGGCAGGATCGTCTTGTCCGGGAAGCCGGCAGGC
                     AGTAGCGGAAGCAGTATTATACCTGACAGGGAGGTTCTCTACCAGGAGTTCGAAGATG
                     TCGTGCCATGCTCAATGGGCTCGACCGGTGCCACCATG"
                     /label="TATCCCTACGATGTGCCCGATTACGCTGCGCCCATCACGGCGTACGCCCA
                     GCAGACGAGAGGCCTCCTAGGGTGTATAATCACCAGCCTGACTGGCCGGGACAAAAAC
                     CAAGTGGAGGGTGAGGTCCAGATCGTGTCAACTGCTACCCAAACCTTCCTGGCAACGT
                     GCATCAATGGGGTATGCTGGGCAGTCTACCACGGGGCCGGAACGAGGACCATCGCATC
                     ACCCAAGGGTCCTGTCATCCAGATGTATACCAATGTGGACCAAGACCTTGTGGGCTGG
                     CCCGCTCCTCAAGGTTCCCGCTCATTGACACCCTGTACCTGCGGCTCCTCGGACCTTT
                     ACCTGGTCACGAGGCACGCCGATGTCATTCCCGTGCGCCGGCGAGGTGATAGCAGGGG
                     TAGCCTGCTTTCGCCCCGGCCCATTTCCTACTTGAAAGGCTCCTCGGGGGGTCCGCTG
                     TTGTGCCCCGCGGGACACGCCGTGGGCCTATTCAGGGCCGCGGTGTGCACCCGTGGAG
                     TGGCTAAAGCGGTGGACTTTATCCCTGTGGAGAACCTAGAGACAACCATGAGATCCCC
                     GGTGTTCACGGACAACTCCTCTCCACCAGCAGTCACCCTGACGCACCCAATCACCAAA
                     ATCGATACCAAATACATCATGACATGCATGTCGGCCGACCTGGAGGTCGTCACGAGCA
                     CCTGGGTGCTCGTTGGCGGCGTCCTGGCTGCTCTGGCCGCGTATTGCCTGTCAACAGG
                     CTGCGTGGTCATAGTGGGCAGGATCGTCTTGTCCGGGAAGCCGGCAGGCAGTAGCGGA
                     AGCAGTATTATACCTGACAGGGAGGTTCTCTACCAGGAGTTCGAAGATGTCGTGCCAT
                     GCTCAATGGGCTCGACCGGTGCCACCATG"
                     /note="Geneious type: Editing History Deletion"
     tag             1541..1561
                     /created_by="User"
                     /modified_by="User"
                     /label="SV40 largeT-antigen NLS"
     CDS             1586..2359
                     /created_by="User"
                     /modified_by="User"
                     /label="SpCas9-1"
     CDS_2           2363..3031
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1"
     CDS             3038..5998
                     /created_by="User"
                     /modified_by="User"
                     /label="SpCas9-2"
     primer          4266..4282
                     /created_by="User"
                     /label="P194"
     CDS_2           <6002..>6670
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1"
     mutation        6116..6118
                     /created_by="User"
                     /label="M40I"
     mutation        6176..6178
                     /created_by="User"
                     /label="V60A"
     mutation        6359..6361
                     /created_by="User"
                     /label="D121R"
     mutation        6365..6367
                     /created_by="User"
                     /label="V123T"
     mutation        6431..>6433
                     /created_by="User"
                     /label="K145N"
     mutation        6470..6472
                     /created_by="User"
                     /label="N158V"
     CDS             6677..7036
                     /created_by="User"
                     /modified_by="User"
                     /label="SpCas9-3"
     misc_feature    7069
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     signaling       7082..7102
                     /created_by="User"
                     /label="NLS"
     misc_feature    7103..7113
                     /Original_Bases="AGGCAAAAAAGAAAAAGGAATT"
                     /label="AGGCAAAAAAGAAAAAGGAATT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7115..7120
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     peptide         7121..7183
                     /created_by="User"
                     /label="T2A"
     antibiotic      7184..7783
                     /created_by="User"
                     /modified_by="User"
                     /label="puroR"
     antibiotic      9090..9950
                     /created_by="User"
                     /modified_by="User"
                     /label="Ampicillin"
     misc_feature    
                     /Original_Bases="ACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAG
                     CGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT
                     CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCT
                     CGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCT
                     GGCCTTTTGCTGGCCTTTTGCTCACATGT"
                     /label="ACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGT
                     CGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGT
                     CCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGG
                     GGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTG
                     CTGGCCTTTTGCTCACATGT"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
       61 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      121 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      181 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      241 atgtttcgaa gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
      301 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
      361 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
      421 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      481 gtggaaagga cgaaacaccg ggagaacagc actccgctct gtttaagagc tatgctggaa
      541 acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      601 cggtgctttt tttgttttag agctagaaat agcaagttaa aatctagagg tacccgttac
      661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      721 aatagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      781 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      841 tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg actttcctac
      901 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
      961 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     1021 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc
     1081 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     1141 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     1201 gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg ccccgctccg
     1261 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     1321 ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa gggtttaagg
     1381 gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct gaaatcactt
     1441 tttttcaggt tggaccggtg ccaccatgga ctataaggac cacgacggag actacaagga
     1501 tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga agcggaaggt
     1561 cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg acatcggcac
     1621 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     1681 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggcg ccctgctgtt
     1741 cgacagcgga gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     1801 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     1861 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     1921 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     1981 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     2041 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     2101 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     2161 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     2221 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     2281 gaagaatggc ctgttcggca acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     2341 gagcaacttc gacctggcca ctagcgtgat caagcccgac atgaaaatca agctgaggat
     2401 ggagggtgcc gtgaacggtc acccgtttgc gatcgagggc gtgggactgg gcaagccgtt
     2461 cgagggaaag caaagcatcg acctcaaggt gaaggagggc ggaccgctgc ccttcgccta
     2521 cgacattctg accactgcct tctgctacgg taatcgcgtc ttcgcaaagt atcccgaaaa
     2581 catcgtggat tactttaagc agagcttccc ggagggttac tcctgggagc gatccatgaa
     2641 ctacgaggac ggagggatct gcaacgcgac gaacgatata accttggacg gcgactgcta
     2701 catttacgaa attcggttcc gcggcaccaa tttcccagcg aatggccctg taatgcaaaa
     2761 acgcacggta aagtgggagc ccagcaccga gaacctgtac gtccgcgacg gagtcctgaa
     2821 gggagacgtg gtcatggcgc tttcattgga aggtggcggg cactacagat gcgatttcaa
     2881 gacgacctat aaggcgaaaa aggtggtcca actgccggac taccatttcg tcgaccacca
     2941 catcgagatc aagagccacg acaaagacta tagcaacgtg aatctccacg agcatgccga
     3001 ggcccacagc gagctgccca ggcaggccaa gactagtgag gatgccaaac tgcagctgag
     3061 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
     3121 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
     3181 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
     3241 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
     3301 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacatcg atggcggagc
     3361 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
     3421 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
     3481 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
     3541 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
     3601 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
     3661 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgccag
     3721 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
     3781 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt
     3841 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc
     3901 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
     3961 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
     4021 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
     4081 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
     4141 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
     4201 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
     4261 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
     4321 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
     4381 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
     4441 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
     4501 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
     4561 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
     4621 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
     4681 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
     4741 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct
     4801 gaaggacgac tccatcgata acaaagtgct gactcggagc gacaagaacc ggggcaagag
     4861 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc gccagctgct
     4921 gaatgccaag ctgattaccc agaggaagtt cgacaatctg accaaggccg agagaggcgg
     4981 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
     5041 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaacga
     5101 caaactgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
     5161 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
     5221 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
     5281 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
     5341 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
     5401 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
     5461 cgaaacaggc gagatcgtgt gggataaggg ccgggacttt gccaccgtgc ggaaagtgct
     5521 gtctatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
     5581 agagtctatc ctgcccaaga ggaacagcga caagctgatc gccagaaaga aggactggga
     5641 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
     5701 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
     5761 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
     5821 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
     5881 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
     5941 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
     6001 aagtgtgatt aaaccagaca tgaagatcaa gctgcgtatg gaaggcgctg taaatggaca
     6061 cccgttcgcg attgaaggag ttggccttgg gaagcctttc gagggaaaac agagtattga
     6121 ccttaaagtc aaagaaggcg gacctctgcc tttcgcctat gacatcttga caactgcttt
     6181 ctgttacggc aacagggtat tcgccaaata cccagaaaat atagtagact atttcaagca
     6241 gtcgtttcct gagggctact cttgggaacg aagcatgaat tacgaagacg ggggcatttg
     6301 taacgcgaca aacgacataa ccctggatgg tgactgttat atctatgaaa ttcgatttag
     6361 aggtacgaac tttcctgcca atggtccagt tatgcagaag aggactgtga aatgggagcc
     6421 atccactgag aacttgtatg tgcgtgatgg agtgctgaag ggtgatgttg tcatggctct
     6481 gtcgcttgaa ggaggtggcc attaccgatg tgacttcaaa actacttata aagctaagaa
     6541 ggttgtccag ttgccagact atcactttgt ggaccaccac attgagatta aaagccacga
     6601 caaagattac agtaatgtta atctgcatga gcacgccgaa gcgcattctg agctgccgag
     6661 gcaggccaag ggatcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     6721 acactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     6781 cgacgctaat ctggacaagg tgctgagcgc ctacaacaag cacagagaca agcctatcag
     6841 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     6901 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     6961 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     7021 tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag gccggccaag ctagcggctc
     7081 ccccaagaaa aaacgcaagg tgggcttaat taacggaagc ggcagtggag agggcagagg
     7141 aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaatgaccg agtacaagcc
     7201 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     7261 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     7321 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7381 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7441 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7501 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7561 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7621 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgcccca
     7681 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7741 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagaattct aactagagct
     7801 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7981 agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc gcaggaaccc
     8041 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     8101 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     8161 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8221 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     8281 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8341 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8401 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8461 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8521 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8581 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8641 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     8701 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     8761 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     8821 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     8881 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     8941 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9001 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9061 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9121 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9181 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9241 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9301 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     9361 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9421 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9481 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9541 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9601 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9661 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9721 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9781 ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg
     9841 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9901 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9961 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10021 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10141 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10261 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10441 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10501 tacagcgtga gctatgagaa agcgcc
//
