LOCUS       916-VPR-dSpCas9        13736 bp ds-DNA     circular     23-SEP-2017
DEFINITION  7930 bp ds-DNA circular SYN 20-JAN-2014.
ACCESSION   urn.local...83byn1n-3g
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     promoter        8..283
                     /gene="CBAp"
                     /note="PROMOTER chicken beta-actin promoter"
                     /note="chicken beta-actin promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CBAp"
     promoter        13..1243
                     /gene="CAGp"
                     /note="PROMOTER CAG promoter"
                     /note="CAG promoter"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CAGp"
     misc_feature    1356..1958
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="PuroR"
     enhancer        1995..2586
                     /vntifkey=9
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE"
     misc_feature    2596..3121
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="PGK Promoter"
     misc_feature    2855
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SpeI mutation"
     misc_feature    3137..3249
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="VP64"
     misc_feature    3137..3249
                     /ApEinfo_fwdcolor="#83ff61"
                     /ApEinfo_revcolor="#83ff61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="VP64 AD domain"
     misc_feature    3251..3292
                     /ApEinfo_label="VP64"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="VP64(1)"
     misc_feature    3251..3292
                     /ApEinfo_label="VP64 AD domain"
                     /ApEinfo_fwdcolor="#83ff61"
                     /ApEinfo_revcolor="#83ff61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="VP64 AD domain(1)"
     misc_feature    3293..3313
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Cas9"
     misc_feature    3293..3313
                     /ApEinfo_fwdcolor="#ff986a"
                     /ApEinfo_revcolor="#ff986a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NLS signal"
     misc_feature    3320..4114
                     /ApEinfo_fwdcolor="#55ffa9"
                     /ApEinfo_revcolor="#55ffa9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="p65 AD domain"
     misc_feature    3525..3528
                     /ApEinfo_fwdcolor="#83b8ff"
                     /ApEinfo_revcolor="#83b8ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AP1 TRE element"
     misc_feature    complement(3580..3583)
                     /ApEinfo_label="AP1 TRE element"
                     /ApEinfo_fwdcolor="#83b8ff"
                     /ApEinfo_revcolor="#83b8ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AP1 TRE element(2)"
     misc_feature    3805..3810
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar"
     misc_feature    complement(3805..3810)
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar"
     misc_feature    4044..4047
                     /ApEinfo_label="AP1 TRE element"
                     /ApEinfo_fwdcolor="#83b8ff"
                     /ApEinfo_revcolor="#83b8ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AP1 TRE element(1)"
     misc_feature    complement(4087..4090)
                     /ApEinfo_label="AP1 TRE element"
                     /ApEinfo_fwdcolor="#83b8ff"
                     /ApEinfo_revcolor="#83b8ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AP1 TRE element(3)"
     misc_feature    4115..4132
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="linker"
     misc_feature    4133..4702
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="RTA"
     misc_feature    4363
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4408
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     CDS             4718..5491
                     /created_by="User"
                     /modified_by="User"
                     /label="dSpCas9-1"
     mutation        4742..4744
                     /created_by="User"
                     /label="D10A"
     misc_feature    4743
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS_2           5495..6163
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1.2"
     CDS             6170..9130
                     /created_by="User"
                     /modified_by="User"
                     /label="dSpCas9-2"
     misc_feature    7910..7911
                     /Original_Bases="CA"
                     /label="CA"
                     /note="Geneious type: Editing History Replacement"
     mutation        7910..7912
                     /created_by="User"
                     /label="H840A"
     CDS_2           <9134..>9802
                     /created_by="User"
                     /modified_by="User"
                     /label="pdDronpa1.2"
     mutation        9248..9250
                     /created_by="User"
                     /label="M40I"
     mutation        9308..9310
                     /created_by="User"
                     /label="V60A"
     mutation        9491..9493
                     /created_by="User"
                     /label="D121R"
     mutation        9497..9499
                     /created_by="User"
                     /label="V123T"
     mutation        9563..>9564
                     /created_by="User"
                     /modified_by="User"
                     /label="145N"
     mutation        9602..9604
                     /created_by="User"
                     /modified_by="User"
                     /label="N158I"
     cds             complement(<9803..9818)
                     /created_by="User"
                     /label
     CDS             9809..10168
                     /created_by="User"
                     /modified_by="User"
                     /label="dSpCas9-3"
     signaling       10214..10234
                     /created_by="User"
                     /label="NLS"
     promoter        10249..10496
                     /gene="3' PB"
                     /note="PROMOTER 3' PB repeat"
                     /note="3' PB repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="3' PB"
     promoter        complement(10537..10555)
                     /gene="T3p"
                     /note="PROMOTER T3 promoter promoter for bacteriophage T3
                     R NA polymerase"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="T3p"
     misc_feature    complement(10576..10592)
                     /gene="M13R"
                     /note="M13 rev common sequencing primer, one of multiple
                     si milar variants"
                     /note="common sequencing primer, one of multiple similar
                     va riants"
                     /ApEinfo_fwdcolor="#cd3249"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13R"
     prot_bind       10600..10616
                     /gene="LacI"
                     /note="PROTEIN_BIND lac repressor encoded by lacI lac
                     opera tor The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved b y adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhib it transcription in E. coli. This inhibition can be
                     relieve d by adding lactose or
                     isopropyl-beta-D-thiogalactopyranosi de (IPTG)."
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="LacI"
     promoter        complement(10624..10654)
                     /gene="LACp"
                     /note="PROMOTER lac promoter promoter for the E. coli lac
                     o peron"
                     /note="promoter for the E. coli lac operon"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="LACp"
     prot_bind       10669..10690
                     /gene="CAPbs"
                     /note="PROTEIN_BIND E. coli catabolite activator protein
                     CA P binding site CAP binding activates transcription in
                     the p resence of cAMP."
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CAPbs"
     rep_origin      complement(10978..11566)
                     /gene="ORI"
                     /note="REP_ORIGIN ori high-copy-number
                     ColE1/pMB1/pBR322/pU C origin of replication"
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     rep lication"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ORI"
     CDS             complement(11737..12597)
                     /gene="AmpR"
                     /note="CDS beta-lactamase AmpR confers resistance to
                     ampici llin, carbenicillin, and related antibiotics"
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGAS LIKHW"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="beta-lactamase"
     promoter        complement(12598..12702)
                     /gene="AmpRp"
                     /note="PROMOTER AmpR promoter"
                     /note="AmpR promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpRp"
     rep_origin      complement(12728..13183)
                     /gene="ORI"
                     /note="REP_ORIGIN f1 ori f1 bacteriophage origin of
                     replica tion; arrow indicates direction of (+) strand
                     synthesis"
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indica tes direction of (+) strand synthesis"
                     /ApEinfo_label="ORI"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ORI(1)"
     misc_feature    13325..13341
                     /gene="M13F"
                     /note="M13 fwd common sequencing primer, one of multiple
                     si milar variants"
                     /note="common sequencing primer, one of multiple similar
                     va riants"
                     /ApEinfo_fwdcolor="#cd3249"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13F"
     promoter        13351..13369
                     /gene="T7p"
                     /note="PROMOTER T7 promoter promoter for bacteriophage T7
                     R NA polymerase"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="T7p"
     promoter        13395..13716
                     /gene="5' PB"
                     /note="PROMOTER 5' PB repeat"
                     /note="5' PB repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="5' PB"
ORIGIN      
        1 ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
       61 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      121 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
      181 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      241 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc
      301 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc
      361 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
      421 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga
      481 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
      541 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
      601 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
      661 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
      721 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
      781 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
      841 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
      901 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag
     1081 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1141 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg
     1201 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     1261 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttgttgtgc
     1321 tgtctcatca ttttggcaaa gaattcgcca ccatgaccat gaccgagtac aagcccacgg
     1381 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     1441 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     1501 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     1561 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     1621 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     1681 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     1741 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     1801 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     1861 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     1921 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt ttaaaccatg caagcttgat
     1981 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2041 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2101 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2161 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2221 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2281 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2341 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2401 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2461 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2521 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2581 ccgcatcctg caggtaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     2641 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     2701 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     2761 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     2821 ggacgtgaca aatggaagta gcacgtctca ctaggctcgt gcagatggac agcaccgctg
     2881 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     2941 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3001 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3061 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     3121 tgtcgacgcc accatggatg ctttagacga ttttgactta gatatgcttg gttcagacgc
     3181 gttagacgac ttcgacctag acatgttagg ctcagatgca ttggacgact tcgatttaga
     3241 tatgttgggc tccgatgccc tagatgactt tgatctagat atgctaggta gtcccaaaaa
     3301 gaagaggaaa gtgcccgggc agtatctgcc cgacacagat gatagacacc gaatcgaaga
     3361 gaaacgcaag cgaacgtatg aaaccttcaa atcgatcatg aagaaatcgc ccttctcggg
     3421 tccgaccgat cccaggcccc caccgagaag gattgcggtc ccgtcccgct cgtcggccag
     3481 cgtgccgaag cctgcgccgc agccctaccc cttcacgtcg agcctgagca caatcaatta
     3541 tgacgagttc ccgacgatgg tgttcccctc gggacaaatc tcacaagcct cggcgctcgc
     3601 accagcgcct ccccaagtcc ttccgcaagc gcctgcccca gcgcctgcac cggcaatggt
     3661 gtccgccctc gcacaggccc ctgcgcccgt ccccgtgctc gcgcctggac cgccccaggc
     3721 ggtcgctcca ccggctccga agccgacgca ggccggagag ggaacactct ccgaagcact
     3781 tcttcaactc cagtttgatg acgaggatct tggagcactc cttggaaact cgacagaccc
     3841 tgcggtgttt accgacctcg cgtcagtaga taactccgaa tttcagcagc ttttgaacca
     3901 gggtatcccg gtcgcgccac atacaacgga gcccatgttg atggaatacc ccgaagcaat
     3961 cacgagactt gtgacgggag cgcagcggcc tcccgatccc gcacccgcac ctttgggggc
     4021 acctggcctc cctaacggac ttttgagcgg cgacgaggat ttctcctcca tcgccgatat
     4081 ggatttctca gccttgctgt cacagatttc cagcggctct ggcagcggca gccgggattc
     4141 cagggaaggg atgtttttgc cgaagcctga ggccggctcc gctattagtg acgtgtttga
     4201 gggccgcgag gtgtgccagc caaaacgaat ccggccattt catcctccag gaagtccatg
     4261 ggccaaccgc ccactccccg ccagcctcgc accaacacca accggtccag tacatgagcc
     4321 agtcgggtca ctgaccccgg caccagtccc tcagccactg gacccagcgc ccgcagtgac
     4381 tcccgaggcc agtcacctgt tggaggaccc cgatgaagag acgagccagg ctgtcaaagc
     4441 ccttcgggag atggccgata ctgtgattcc ccagaaggaa gaggctgcaa tctgtggcca
     4501 aatggacctt tcccatccgc ccccaagggg ccatctggat gagctgacaa ccacacttga
     4561 gtccatgacc gaggatctga acctggactc acccctgacc ccggaattga acgagattct
     4621 ggataccttc ctgaacgacg agtgcctctt gcatgccatg catatcagca caggactgtc
     4681 catcttcgac acatctctgt ttcatatgtc aagcatggac aagaagtaca gcatcggcct
     4741 ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
     4801 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     4861 cgccctgctg ttcgacagcg gagaaacagc cgaggccacc cggctgaaga gaaccgccag
     4921 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     4981 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     5041 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     5101 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     5161 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     5221 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
     5281 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
     5341 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
     5401 gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc tgggcctgac
     5461 ccccaacttc aagagcaact tcgacctggc cactagcgtg atcaagcccg acatgaaaat
     5521 caagctgagg atggagggtg ccgtgaacgg tcacccgttt gcgatcgagg gcgtgggact
     5581 gggcaagccg ttcgagggaa agcaaagcat cgacctcaag gtgaaggagg gcggaccgct
     5641 gcccttcgcc tacgacattc tgaccactgc cttctgctac ggtaatcgcg tcttcgcaaa
     5701 gtatcccgaa aacatcgtgg attactttaa gcagagcttc ccggagggtt actcctggga
     5761 gcgatccatg aactacgagg acggagggat ctgcaacgcg acgaacgata taaccttgga
     5821 cggcgactgc tacatttacg aaattcggtt ccgcggcacc aatttcccag cgaatggccc
     5881 tgtaatgcaa aaacgcacgg taaagtggga gcccagcacc gagaacctgt acgtccgcga
     5941 cggagtcctg aagggagacg tgatcatggc gctttcattg gaaggtggcg ggcactacag
     6001 atgcgatttc aagacgacct ataaggcgaa aaaggtggtc caactgccgg actaccattt
     6061 cgtcgaccac cacatcgaga tcaagagcca cgacaaagac tatagcaacg tgaatctcca
     6121 cgagcatgcc gaggcccaca gcgagctgcc caggcaggcc aagactagtg aggatgccaa
     6181 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     6241 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     6301 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     6361 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     6421 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     6481 cgatggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     6541 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     6601 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     6661 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     6721 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     6781 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     6841 caagggcgcc agcgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     6901 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtacaacga
     6961 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     7021 gcagaaaaaa gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     7081 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     7141 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     7201 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     7261 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     7321 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     7381 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     7441 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     7501 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     7561 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     7621 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     7681 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     7741 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     7801 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     7861 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
     7921 tcagagcttt ctgaaggacg actccatcga taacaaagtg ctgactcgga gcgacaagaa
     7981 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     8041 gcgccagctg ctgaatgcca agctgattac ccagaggaag ttcgacaatc tgaccaaggc
     8101 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     8161 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     8221 cgacgagaac gacaaactga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     8281 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     8341 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     8401 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     8461 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     8521 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     8581 cgagacaaac ggcgaaacag gcgagatcgt gtgggataag ggccgggact ttgccaccgt
     8641 gcggaaagtg ctgtctatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     8701 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gacaagctga tcgccagaaa
     8761 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     8821 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     8881 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     8941 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     9001 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     9061 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     9121 gaagctgaag ggaagtgtga ttaaaccaga catgaagatc aagctgcgta tggaaggcgc
     9181 tgtaaatgga cacccgttcg cgattgaagg agttggcctt gggaagcctt tcgagggaaa
     9241 acagagtatt gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt
     9301 gacaactgct ttctgttacg gcaacagggt attcgccaaa tacccagaaa atatagtaga
     9361 ctatttcaag cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga attacgaaga
     9421 cgggggcatt tgtaacgcga caaacgacat aaccctggat ggtgactgtt atatctatga
     9481 aattcgattt agaggtacga actttcctgc caatggtcca gttatgcaga agaggactgt
     9541 gaaatgggag ccatccactg agaacttgta tgtgcgtgat ggagtgctga agggtgatgt
     9601 tatcatggct ctgtcgcttg aaggaggtgg ccattaccga tgtgacttca aaactactta
     9661 taaagctaag aaggttgtcc agttgccaga ctatcacttt gtggaccacc acattgagat
     9721 taaaagccac gacaaagatt acagtaatgt taatctgcat gagcacgccg aagcgcattc
     9781 tgagctgccg aggcaggcca agggatcccc cgaggataat gagcagaaac agctgtttgt
     9841 ggaacagcac aaacactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     9901 agtgatcctg gccgacgcta atctggacaa ggtgctgagc gcctacaaca agcacagaga
     9961 caagcctatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
    10021 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
    10081 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
    10141 gatcgacctg tctcagctgg gaggcgacaa aaggccggcg gccacgaaaa aggccggcca
    10201 agctagcggc tcccccaaga aaaaacgcaa ggtgtaactc gagctagtta aaagttttgt
    10261 tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata
    10321 aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta
    10381 ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg
    10441 catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa tctagtatac
    10501 gcgtatgcgg ccgcttaatt aatccagctt ttgttccctt tagtgagggt taattgcgcg
    10561 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10621 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10681 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10741 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11401 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11701 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11761 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11821 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11881 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11941 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12001 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12061 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12121 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12181 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12241 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12301 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12361 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12421 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12481 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12541 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12721 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
    12781 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
    12841 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
    12901 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
    12961 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    13021 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    13081 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    13141 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
    13201 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    13261 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    13321 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggg
    13381 cgcgccattc tagattaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt
    13441 gaaatattgc tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc
    13501 agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt
    13561 gaactataac gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag
    13621 atttaactca tacgataatt atattgttat ttcatgttct acttacgtga taacttatta
    13681 tatatatatt ttcttgttat agatatcaac tagaatgcta gcatgggccc agatct
//
