LOCUS GG_EBNA_EEA_guid 9360 bp ds-DNA circular 01-OCT-2017 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT >pX335-dCas9-3xVP16-PGK-puro-EBNA1-OriP (14377bp) COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb complement(3199..3232) /label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(6971..7004) /label=LoxP(1) /ApEinfo_label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3830..4429 /label=Puro /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3236..3827 /label=PGKpromoter /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(7232..7240) /label=Kozak sequence /ApEinfo_fwdcolor=#cc66ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4878..6803 /label=EBNA orf /ApEinfo_fwdcolor=#ff6666 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 7201..9360 /label=EBV origin /ApEinfo_fwdcolor=#cc66ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 758..974 /label=AmpR /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 1515..2197 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 2537..2559 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 975..1417 /label=AmpR(1) /ApEinfo_label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2702..2950 /label=U6 /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#0080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2952..2965 /label=EEA-g1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2966..3041 /label=fused sgRNA /ApEinfo_fwdcolor=#0080c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg tccggggcgg 61 ggcatcgatc ctctagtaag ggattttgcc gatttcggcc tattggttaa aaaatgagct 121 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg 181 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac 241 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 301 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 361 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 421 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 481 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 541 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 601 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 661 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 721 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 781 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 841 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 901 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 961 cttacttctg acaaCGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 1021 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 1081 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 1141 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 1201 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 1261 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 1321 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 1381 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 1441 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 1501 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 1561 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 1621 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 1681 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 1741 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 1801 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 1861 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 1921 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 1981 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 2041 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 2101 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 2161 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 2221 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 2281 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 2341 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 2401 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 2461 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 2521 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 2581 CCATGATTAC GCCAAGCTCT AATACGACTC ACTATAGGGA GACAAGCTTG CATGCCTGCA 2641 GGTCGACTCT AGAGgatctc gagctcaagc ttcGAATTCC CCCTGTAAAA CGACGGCCAG 2701 Tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga 2761 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag 2821 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca 2881 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tGTGGAAAGG 2941 ACGAAACACC gCCCAGCACT TTGGGgtttt agagctaGAA Atagcaagtt aaaataaggc 3001 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cTTTTTTTCA TGGTCATAGC 3061 TGTTTCCTca tgAgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc 3121 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccA ATTCcgatca 3181 tattcaataa cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtctg 3241 aagaggagtt tacgtccagc caagcttagg atctcgacct cgaaattcta ccgggtaggg 3301 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct ggcacttggc 3361 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg 3421 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc 3481 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta 3541 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg 3601 gccaatagca gctttgctcc ttcgctttct gggctcagca gctgggaagg gtgggtccgg 3661 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc 3721 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc 3781 gggcctttcg acctgcatcc atctagatct cgagcagctg aagcttacca tgaccgagta 3841 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc 3901 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga 3961 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 4021 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 4081 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 4141 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 4201 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 4261 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 4321 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 4381 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca 4441 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgataa gctgatcctc 4501 acaggccgca cccagctttt cttccgttgc cccagtagca tctctgtctg gtgaccttga 4561 agaggaagag gaggggtccc gagaatcccc atccctaccg tccagcaaaa agggggacga 4621 ggaatttgag gcctggcttg aggctcagga cgcaaatctt gaggatgttc agcgggagtt 4681 ttccgggctg cgagtaattg gtgatgagga cgaggatggt tcggaggatg gggaattttc 4741 agacctggat ctgtctgaca gcgaccatga aggggatgag ggtggggggg ctgttggagg 4801 gggcaggagt ctgcactccc tgtattcact gagcgtcgtc taataaagat gtctattgat 4861 ctcttttagt gtgaatcatg tctgacgagg ggccaggtac aggacctgga aatggcctag 4921 gagagaaggg agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag 4981 ggggtgataa ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag 5041 gagccccggg cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa 5101 aacgtccaag ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag 5161 cgggaggggc aggagcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg 5221 caggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag 5281 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggaggggcag 5341 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg 5401 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag 5461 gggcaggagc aggaggaggg gcaggagcag gaggggcagg aggggcagga ggggcaggag 5521 caggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggagggg 5581 caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga ggggcaggag 5641 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggagggg 5701 caggaggggc aggagcagga ggaggggcag gaggggcagg agcaggaggg gcaggagggg 5761 caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag 5821 gaggggcagg agcaggaggg gcaggagcag gaggtggagg ccggggtcga ggaggcagtg 5881 gaggccgggg tcgaggaggt agtggaggcc ggggtcgagg aggtagtgga ggccgccggg 5941 gtagaggacg tgaaagagcc agggggggaa gtcgtgaaag agccaggggg agaggtcgtg 6001 gacgtggaga aaagaggccc aggagtccca gtagtcagtc atcatcatcc gggtctccac 6061 cgcgcaggcc ccctccaggt agaaggccat ttttccaccc tgtaggggaa gccgattatt 6121 ttgaatacca ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg ggagcgatag 6181 agcagggccc cgcagatgac ccaggagaag gcccaagcac tggaccccgg ggtcagggtg 6241 atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca 6301 acccgaaatt tgagaacatt gcagaaggtt taagagctct cctggctagg agtcacgtag 6361 aaaggactac cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga 6421 cctcccttta caacctaagg cgaggaactg cccttgctat tccacaatgt cgtcttacac 6481 cattgagtcg tctccccttt ggaatggccc ctggacccgg cccacaacct ggcccgctaa 6541 gggagtccat tgtctgttat ttcatggtct ttttacaaac tcatatattt gctgaggttt 6601 tgaaggatgc gattaaggac cttgttatga caaagcccgc tcctacctgc aatatcaggg 6661 tgactgtgtg cagctttgac gatggagtag atttgcctcc ctggtttcca cctatggtgg 6721 aaggggctgc cgcggagggt gatgacggag atgacggaga tgaaggaggt gatggagatg 6781 agggtgagga agggcaggag tgatgtaact tgttaggaga cgccctcaat cgtattaaaa 6841 gccgtgtatt cccccgcact aaagaataaa tccccagtag acatcatgcg tgctgttggt 6901 gtatttctgg ccatctgtct tgtcaccatt ttcgtcctcc caacatgggg caattgccgg 6961 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgaagag 7021 gttcactagc ggatctcaat tgggcatacc catgttgtca cgtcactcag ctccgcgctc 7081 aacaccttct cgcgttggaa aacattagcg acatttacct ggtgagcaat cagacatgcg 7141 acggctttag cctggcctcc ttaaattcac ctaagaatgg gagcaaccag caggaaaagg 7201 acaagcagcg aaaattcacg cccccttggg aggtggcggc atatgcaaag gatagcactc 7261 ccactctact actgggtatc atatgctgac tgtatatgca tgaggatagc atatgctacc 7321 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc 7381 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc 7441 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc 7501 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc atatgctatc 7561 cagatatttg ggtagtatat gctacccaga tataaattag gatagcatat actaccctaa 7621 tctctattag gatagcatat gctacccgga tacagattag gatagcatat actacccaga 7681 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga 7741 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga 7801 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctatccaga 7861 tatttgggta gtatatgcta cccatggcaa cattagccca ccgtgctctc agcgacctcg 7921 tgaatatgag gaccaacaac cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc 7981 ctccagatcg cagcaatcgc gcccctatct tggcccgccc acctacttat gcaggtattc 8041 cccggggtgc cattagtggt tttgtgggca agtggtttga ccgcagtggt tagcggggtt 8101 acaatcagcc aagttattac acccttattt tacagtccaa aaccgcaggg cggcgtgtgg 8161 gggctgacgc gtgcccccac tccacaattt caaaaaaaag agtggccact tgtctttgtt 8221 tatgggcccc attggcgtgg agccccgttt aattttcggg ggtgttagag acaaccagtg 8281 gagtccgctg ctgtcggcgt ccactctctt tccccttgtt acaaatagag tgtaacaaca 8341 tggttcacct gtcttggtcc ctgcctggga cacatcttaa taaccccagt atcatattgc 8401 actaggatta tgtgttgccc atagccataa attcgtgtga gatggacatc cagtctttac 8461 ggcttgtccc caccccatgg atttctattg ttaaagatat tcagaatgtt tcattcctac 8521 actagtattt attgcccaag gggtttgtga gggttatatt ggtgtcatag cacaatgcca 8581 ccactgaacc ccccgtccaa attttattct gggggcgtca cctgaaacct tgttttcgag 8641 cacctcacat acaccttact gttcacaact cagcagttat tctattagct aaacgaagga 8701 gaatgaagaa gcaggcgaag attcaggaga gttcactgcc cgctccttga tcttcagcca 8761 ctgcccttgt gactaaaatg gttcactacc ctcgtggaat cctgacccca tgtaaataaa 8821 accgtgacag ctcatggggt gggagatatc gctgttcctt aggacccttt tactaaccct 8881 aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg gttagtctgg 8941 atagtatata ctactacccg ggaagcatat gctacccgtt tagggttaac aagggggcct 9001 tataaacact attgctaatg ccctcttgag ggtccgctta tcggtagcta cacaggcccc 9061 tctgattgac gttggtgtag cctcccgtag tcttcctggg cccctgggag gtacatgtcc 9121 cccagcattg gtgtaagagc ttcagccaag agttacacat aaaggcaatg ttgtgttgca 9181 gtccacagac tgcaaagtct gctccaggat gaaagccact cagtgttggc aaatgtgcac 9241 atccatttat aaggatgtca actacagtca gagaacccct ttgtgtttgg tccccccccg 9301 tgtcacatgt ggaacagggc ccagttggca agttgtacca accaactgaa gggattacat //