LOCUS       Exported                5388 bp ds-DNA     circular SYN 07-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 7, 2017 from SnapGene Viewer 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    216..515
                     /label=oriV
                     /note="/ApEinfo_fwdcolor=#400000
                     /ApEinfo_graphicformat=arrow_data {{} {} 0} width 8 offset
                     0
                     /ApEinfo_revcolor=#400000"
     misc_feature    complement(2018..2644)
                     /label=tetR
                     /note="/ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 8 offset 0
                     /ApEinfo_revcolor=#c0c0c0"
     misc_feature    complement(2645..2694)
                     /label=PtetR
                     /note="/ApEinfo_fwdcolor=#2f2f2f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 8 offset 0
                     /ApEinfo_revcolor=#2f2f2f"
     misc_feature    2705..2784
                     /label=PtetO
                     /note="/ApEinfo_fwdcolor=#800080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 8 offset 0
                     /ApEinfo_revcolor=#800080"
     misc_feature    2817..2835
                     /label=Consensus RBS
                     /note="/ApEinfo_fwdcolor=#ff9919
                     /ApEinfo_graphicformat=arrow_data {{} {} 0} width 5 offset
                     0
                     /ApEinfo_revcolor=#ff9919"
     misc_feature    2836..4176
                     /label=LmrR-BzdB1_nSP
                     /note="/ApEinfo_fwdcolor=#ffcf00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 6 offset 0
                     /ApEinfo_revcolor=#ffcf00"
     misc_feature    complement(4517..5176)
                     /label=Cmr
                     /note="/ApEinfo_fwdcolor=#808000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 8 offset 0
                     /ApEinfo_revcolor=#808000"
ORIGIN
        1 ctgtagtgcc atttaccccc attcactgcc agagccgtga gcgcagcgaa ctgaatgtca
       61 cgaaaaagac agcgacttag gtgcctgatg gtcggagaca aaaggaatat tcagcgattt
      121 gcccgagctt gcgagggtgc tacttaagcc tttagggttt taaggtctgt tttgtagagg
      181 agcaaacagc gtttgcgaca tccttttgta atactgcgga actgactaaa gtagtgagtt
      241 atacacaggg ctgggatcta ttctttttat ctttttttat tctttcttta ttctataaat
      301 tataaccact tgaatataaa caaaaaaaac acacaaaggt ctagcggaat ttacagaggg
      361 tctagcagaa tttacaagtt ttccagcaaa ggtctagcag aatttacaga tacccacaac
      421 tcaaaggaaa aggactagta attatcattg actagcccat ctcaattggt atagtgatta
      481 aaatcaccta gaccaattga gatgtatgtc tgaattagtt gttttcaaag caaatgaact
      541 agcgattagt cgctatgact taacggagca tgaaaccaag ctaattttat gctgtgtggc
      601 actactcaac cccacgattg aaaaccctac aaggaaagaa cggacggtat cgttcactta
      661 taaccaatac gctcagatga tgaacatcag tagggaaaat gcttatggtg tattagctaa
      721 agcaaccaga gagctgatga cgagaactgt ggaaatcagg aatcctttgg ttaaaggctt
      781 tgagattttc cagtggacaa actatgccaa gttctcaagc gaaaaattag aattagtttt
      841 tagtgaagag atattgcctt atcttttcca gttaaaaaaa ttcataaaat ataatctgga
      901 acatgttaag tcttttgaaa acaaatactc tatgaggatt tatgagtggt tattaaaaga
      961 actaacacaa aagaaaactc acaaggcaaa tatagagatt agccttgatg aatttaagtt
     1021 catgttaatg cttgaaaata actaccatga gtttaaaagg cttaaccaat gggttttgaa
     1081 accaataagt aaagatttaa acacttacag caatatgaaa ttggtggttg ataagcgagg
     1141 ccgcccgact gatacgttga ttttccaagt tgaactagat agacaaatgg atctcgtaac
     1201 cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa ataccaacaa ccattacatc
     1261 agattcctac ctacataacg gactaagaaa aacactacac gatgctttaa ctgcaaaaat
     1321 tcagctcacc agttttgagg caaaattttt gagtgacatg caaagtaagt atgatctcaa
     1381 tggttcgttc tcatggctca cgcaaaaaca acgaaccaca ctagagaaca tactggctaa
     1441 atacggaagg atctgaggtt cttatggctc ttgtatctat cagtgaagca tcaagactaa
     1501 caaacaaaag tagaacaact gttcaccgtt acatatcaaa gggaaaactg tccatatgca
     1561 cagatgaaaa cggtgtaaaa aagatagata catcagagct tttacgagtt tttggtgcat
     1621 ttaaagctgt tcaccatgaa cagatcgaca atgtaacaga tgaacagcat gtaacaccta
     1681 atagaacagg tgaaaccagt aaaacaaagc aactagaaca tgaaattgaa cacctgagac
     1741 aacttgttac agctcaacag tcacacatag acagcctgaa acaggcgatg ctgcttatcg
     1801 aatcaaagct gccgacaaca cgggagccag tgacgcctcc cgtggggaaa aaatcatggc
     1861 aattctggaa gaaatagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1921 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     1981 ttaatgtgag ttagctcact cattaggcac cccaggctta agacccactt tcacatttaa
     2041 gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct ggctctgcac
     2101 cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta tcagtagtag
     2161 gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg caacctaaag
     2221 taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa ccttgttggc
     2281 ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc cgtgtaccta
     2341 aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt ttagcgttat
     2401 tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta tggtgcctat
     2461 ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca tgccaataca
     2521 atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa ccttcgattc
     2581 cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct aatctagaca
     2641 tcattaattc ctaatttttg ggtctactct atcattgata gcgtgccttt accactccct
     2701 atcatcgttt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag
     2761 cacatcagca ggacgcactg accggcggcc gcgcgaattc gagctcggta cccgggtgac
     2821 ctaaggaggt aaataatggc agaaatacca aaagaaatgt tacgagccca aaccaatgta
     2881 attttgctca atgtcctaaa acaaggagat aattatgttt acggtattat caaacaagtc
     2941 aaggaagcct cgaatggaga aatggaactt aatgaagcca cgctctatac gatctttaaa
     3001 agacttgaaa aggatgggat tatcagttct tattggggag atgaaagtca aggcgggcgt
     3061 cgcaagtatt accgactgca gagcgcaaat aaaattcgtg tgggtttaat gctgccttac
     3121 accggtacct atgcgagcct gggtaatgcc attaccaatg gctttaaact ggccgttgaa
     3181 caaggtggtg gtaaactggg tggtcgcgag attgagtact tcaccgtgga tgatgaaagc
     3241 gatgccgcaa aagcgccgga aaatgccaac aaactgatta aacgtgataa tgtggatgtg
     3301 ctgattggca ccgttcatag cggcgttgca ctggcaatga cccgtgttgc acgtgaaacc
     3361 aaaacactgc tgatcgttcc gaatgcgggt gcagatgaaa ttaccggtcc gctgtgtagc
     3421 ccgaatattt ttcgtacaag ctttagcgca tggcagccgg catatgcaat gggtcaggtg
     3481 atggccgaaa aaaaacataa aaatgtggtg accctgagct ggaaatatag cttcggcgaa
     3541 cagagcgtgg caggtttcaa ggaagccttt gaaaaaggcg gcggcaaagt ggttaaagaa
     3601 atgtatctgc cgtttccgaa cgtggaattt cagccgtatc tgacggaaat tgccgcactg
     3661 aaaccggatg ccgtgtttgt gttttttgcc ggcggtggtg cagtgaaatt tgtgaaagat
     3721 tatgacgccg caggtctgaa aaaaaccatt ccgctgtatg gtagcggttt tctgaccgat
     3781 ggtaccctgg aagcacaggg tgatagcgca gaaggtattc tgaccaccct gcattatgca
     3841 gatggtctgg atattgcaaa agataaagcc tttcgtaccg cctatgccat ggccttcaaa
     3901 acccagccgg atgtttatag cgttcagggt tatgatgcag cacagctttt tgcagcaggt
     3961 ttagcgggtg ttgccggtga tgttaccaaa cgtgaagcac tgattgcagc aatggaaaaa
     4021 gcaaccattg atagcccgcg tggtcgtttt accctgagta aagcacataa tccggtgcag
     4081 gatatttacc tgcggaaagt ggaagccaaa cagaataaag tgatcgcagt ggcagcaaaa
     4141 gccctggcag atccggcacg tggttgtcgt atgtaagatc ctctagagtg gacctgcagg
     4201 catgcaagct tgcggccgcc cgtttttatc aggctctggg aggcagaata aatgatcata
     4261 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
     4321 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
     4381 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
     4441 cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat
     4501 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     4561 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     4621 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     4681 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     4741 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     4801 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     4861 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     4921 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     4981 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     5041 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     5101 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     5161 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     5221 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     5281 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     5341 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgc
//
