LOCUS Exported 8308 bp ds-DNA circular SYN 23-JAN-2018 DEFINITION -. ACCESSION - VERSION . KEYWORDS - SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8308) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 24, 2018 from SnapGene 4.2.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8308 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 67..184 /note="color: #a6acb3" CDS 715..1314 /label=Puro /note="/vntifkey=""4""" /note="color: #993366; direction: RIGHT" primer_bind 1776..1794 /label=FLORO F /note="FLORO F" /note="color: #a020f0; direction: RIGHT" CDS 1954..2664 /label=mCherry /note="mCherry" /note="color: #993366; direction: RIGHT" primer_bind complement(2640..2672) /label=mCherry_PacI_R3 bind /note="mCherry_PacI_R3 bind" /note="color: #a020f0; direction: LEFT" primer_bind complement(2747..2766) /label=FLORO_R /note="FLORO_R" /note="color: #a020f0; direction: LEFT" LTR 2792..3275 /label=3'LTR /note="/vntifkey=""19""" /note="color: #ffe4c4" CDS 4606..5466 /label=Amp /note="/vntifkey=""4""" /note="color: #993366; direction: RIGHT" primer_bind 6385..6404 /label=FLORO_R /note="FLORO_R" /note="color: #a020f0; direction: RIGHT" LTR 6401..6915 /label=5'LTR /note="/vntifkey=""19""" /note="color: #ffe4c4" misc_feature 6916..7804 /label=Psi+ /note="/vntifkey=""21""" /note="color: #a6acb3" primer_bind 7733..7755 /label=MSCVseqF /note="/vntifkey=""28""" /note="color: #a020f0; direction: RIGHT" primer_bind 7748..7767 /label=GAG /note="GAG" /note="color: #a020f0; direction: RIGHT" misc_feature 7840..7935 /label=miR30.1224 /note="/vntifkey=""21"" [Split]" /note="color: #a6acb3" ORIGIN 1 aggccaaaga tcggaagagc gtcgtgtagg gaaagagtgt agatctcggt ggtcgccgta 61 tcattgaatt caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct 121 tgctatctct ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt 181 ttttcaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt 241 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac 301 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac 361 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca 421 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa 481 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca 541 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg 601 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc 661 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc 721 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 781 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 841 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 901 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 961 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 1021 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 1081 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 1141 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 1201 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 1261 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg 1321 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatcatcc aattccgccc 1381 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat 1441 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct 1501 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg 1561 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta 1621 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag 1681 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg 1741 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat 1801 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca 1861 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 1921 tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga ggaggataac 1981 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 2041 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 2101 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 2161 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 2221 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 2281 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 2341 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 2401 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 2461 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 2521 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 2581 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 2641 ggcatggacg agctgtacaa gtaattaatt aagaattatc aagcttatcg ataccgtcga 2701 cctgcagcca agcttatcga taaaataaaa gattttattt agtctccaga aaaagggggg 2761 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc 2821 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag 2881 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 2941 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt 3001 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt 3061 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac 3121 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa 3181 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc 3241 tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct tgaagttgga 3301 gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat tagccactgt 3361 tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg cagaagagct 3421 ggggagaatt aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 3481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 3541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 3601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 4261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 4501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 4561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 4621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 4681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 4741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 4801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 4861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 4921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 4981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 5041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 5101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 5161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 5221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 5281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 5341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 5401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 5461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 5521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 5581 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 5641 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 5701 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 5761 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 5821 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 5881 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 5941 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 6001 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca 6061 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc 6121 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg 6181 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg 6241 atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag 6301 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata 6361 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg 6421 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa 6481 tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat 6541 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 6601 gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 6661 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 6721 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc 6781 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat 6841 ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct 6901 cgggggtctt tcatttggag gttccaccga gatttggaga cccctgccca gggaccaccg 6961 acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg 7021 cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc 7081 tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg 7141 agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg 7201 tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc 7261 ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg 7321 cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa 7381 aattagggcc agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc 7441 gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc 7501 tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac 7561 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag 7621 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc 7681 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa 7741 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc 7801 gccggaatta gatctctcga ctagggataa cagggtaatt gtttgaatga ggcttcagta 7861 ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact tcttcaggtt 7921 aacccaacag aaggctcgag gtgactggag ttcagacgtg tgctcttccg atcgccacca 7981 tgcctaatca tcagtccggg tcacctaccg gcagttcaga cctgctcctt gatggcaaga 8041 aacaacgagc ccatctggcg ctgaggagaa aacggcgacg ggaaatgcgc aagattaacc 8101 gaaaggtgag aagaatgaat ctcgcaccca ttaaagaaaa aacagcctgg cagcacctgc 8161 aagctctgat cttcgaggcg gaagaagtgt tgaagacttc tcaaactcca cagacctccc 8221 tcacgctgtt tctagcactc ttggccgtat tggccccgcc acctgtgagc gggagcatca 8281 tcaacttcga gaagctgtaa ggccaaac //