LOCUS       Exported                8308 bp ds-DNA     circular SYN 23-JAN-2018
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    -
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2018 from SnapGene 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    67..184
                     /note="color: #a6acb3"
     CDS             715..1314
                     /label=Puro
                     /note="/vntifkey=""4"""
                     /note="color: #993366; direction: RIGHT"
     primer_bind     1776..1794
                     /label=FLORO F
                     /note="FLORO F"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             1954..2664
                     /label=mCherry
                     /note="mCherry"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(2640..2672)
                     /label=mCherry_PacI_R3 bind
                     /note="mCherry_PacI_R3 bind"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(2747..2766)
                     /label=FLORO_R
                     /note="FLORO_R"
                     /note="color: #a020f0; direction: LEFT"
     LTR             2792..3275
                     /label=3'LTR
                     /note="/vntifkey=""19"""
                     /note="color: #ffe4c4"
     CDS             4606..5466
                     /label=Amp
                     /note="/vntifkey=""4"""
                     /note="color: #993366; direction: RIGHT"
     primer_bind     6385..6404
                     /label=FLORO_R
                     /note="FLORO_R"
                     /note="color: #a020f0; direction: RIGHT"
     LTR             6401..6915
                     /label=5'LTR
                     /note="/vntifkey=""19"""
                     /note="color: #ffe4c4"
     misc_feature    6916..7804
                     /label=Psi+
                     /note="/vntifkey=""21"""
                     /note="color: #a6acb3"
     primer_bind     7733..7755
                     /label=MSCVseqF
                     /note="/vntifkey=""28"""
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     7748..7767
                     /label=GAG
                     /note="GAG"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    7840..7935
                     /label=miR30.1224
                     /note="/vntifkey=""21"" [Split]"
                     /note="color: #a6acb3"
ORIGIN
        1 aggccaaaga tcggaagagc gtcgtgtagg gaaagagtgt agatctcggt ggtcgccgta
       61 tcattgaatt caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct
      121 tgctatctct ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt
      181 ttttcaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
      241 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
      301 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
      361 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
      421 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
      481 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
      541 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
      601 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
      661 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
      721 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
      781 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
      841 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
      901 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
      961 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1021 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     1081 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     1141 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     1201 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     1261 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     1321 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatcatcc aattccgccc
     1381 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     1441 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     1501 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     1561 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     1621 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     1681 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     1741 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     1801 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     1861 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     1921 tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga ggaggataac
     1981 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2041 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2101 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2161 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2221 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2281 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2341 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2401 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2461 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2521 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2581 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2641 ggcatggacg agctgtacaa gtaattaatt aagaattatc aagcttatcg ataccgtcga
     2701 cctgcagcca agcttatcga taaaataaaa gattttattt agtctccaga aaaagggggg
     2761 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     2821 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
     2881 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     2941 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
     3001 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     3061 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     3121 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa
     3181 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     3241 tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct tgaagttgga
     3301 gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat tagccactgt
     3361 tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg cagaagagct
     3421 ggggagaatt aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5581 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5641 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     5701 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5761 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     5821 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     5881 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     5941 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     6001 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca
     6061 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     6121 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     6181 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     6241 atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag
     6301 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata
     6361 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     6421 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa
     6481 tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat
     6541 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     6601 gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     6661 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     6721 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
     6781 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
     6841 ccgaatcgtg gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct
     6901 cgggggtctt tcatttggag gttccaccga gatttggaga cccctgccca gggaccaccg
     6961 acccccccgc cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg
     7021 cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc
     7081 tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg
     7141 agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg
     7201 tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc
     7261 ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg
     7321 cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa
     7381 aattagggcc agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc
     7441 gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc
     7501 tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac
     7561 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag
     7621 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc
     7681 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa
     7741 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc
     7801 gccggaatta gatctctcga ctagggataa cagggtaatt gtttgaatga ggcttcagta
     7861 ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact tcttcaggtt
     7921 aacccaacag aaggctcgag gtgactggag ttcagacgtg tgctcttccg atcgccacca
     7981 tgcctaatca tcagtccggg tcacctaccg gcagttcaga cctgctcctt gatggcaaga
     8041 aacaacgagc ccatctggcg ctgaggagaa aacggcgacg ggaaatgcgc aagattaacc
     8101 gaaaggtgag aagaatgaat ctcgcaccca ttaaagaaaa aacagcctgg cagcacctgc
     8161 aagctctgat cttcgaggcg gaagaagtgt tgaagacttc tcaaactcca cagacctccc
     8221 tcacgctgtt tctagcactc ttggccgtat tggccccgcc acctgtgagc gggagcatca
     8281 tcaacttcga gaagctgtaa ggccaaac
//
