LOCUS       Exported                4435 bp ds-DNA     circular SYN 02-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    redSTAGR_short CMV.xdna
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 2, 2017 from SnapGene Viewer 4.0.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4435)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(103..762))
                     /label=AmpR
     promoter        complement(1002..1030))
                     /label=Amp prom
     primer_bind     1774..1793)
                     /label=T7
     misc_feature    1806..3233)
                     /label=TD
     promoter        3327..3589)
                     /label=U6 promoter
     misc_feature    3591
                     /label=Scaffold [5']
     misc_feature    3598..3679)
                     /label=Scaffold [3']
     rep_origin      complement(3758..4386))
                     /direction=LEFT
                     /label=ColE1 origin
BASE COUNT         1110 a         1194 c         1135 g         995 t
ORIGIN
        1 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
       61 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      121 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      181 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      241 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      301 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
      361 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
      421 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
      481 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
      541 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
      601 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
      661 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
      721 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
      781 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
      841 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
      901 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
      961 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1021 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1081 agtgccacct gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagct
     1141 acccggttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     1201 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1261 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1321 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1381 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1441 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1501 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     1561 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1621 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     1681 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     1741 gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata gggagagccg
     1801 ccaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
     1861 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     1921 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     1981 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     2041 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     2101 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     2161 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     2221 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     2281 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     2341 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     2401 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     2461 agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg
     2521 gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg
     2581 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     2641 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     2701 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     2761 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     2821 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     2881 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     2941 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     3001 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     3061 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     3121 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     3181 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtccggac
     3241 tcagatctaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg
     3301 tgctctcctg actggatccg gtaccaaggt cgggcaggaa gagggcctat ttcccatgat
     3361 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     3421 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     3481 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     3541 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg aaaaaagttt
     3601 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     3661 ccgagtcggt gcttttttta agcttgggcc gctcgaggta cctctctaca tatgacatgt
     3721 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3781 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3841 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3901 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3961 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4021 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4081 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4141 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4201 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4261 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4321 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4381 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggt
//
