LOCUS       dCas9-VPR_version_2    13958 bp ds-DNA     circular     26-JUN-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3258..3281
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(6578..6798)
                     /label="URA3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    9701..9719
                     /label="TetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        7628..7656
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(4749..4760)
                     /label="CYC1_primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      4740..4760
                     /label="CYC1_terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3045..3059
                     /label="NLS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    9700..9700
                     /label="TSS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3060..4655
                     /label="VPR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4083..4652
                     /label="RTA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      8653..9335
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3282..4064
                     /label="P65"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    12881..3038
                     /label="dCas9(Church)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(5027..5044)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             11005..11628
                     /label="TetR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    12359..12377
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor=#8f25ff
                     /ApEinfo_fwdcolor=#8f25ff
     promoter        10238..10997
                     /label="pGPM1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4749..4760)
                     /label="pYESTrp_rev_primer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      5188..5643
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    12848..12874
                     /label="NLS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(11997..12026)
                     /label="211_tGPM1_RevPrime"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            7698..8558
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      9792..10229
                     /label="RPR1t"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4761..4989
                     /label="CYC1 Terminator"
                     /ApEinfo_revcolor=#ff47f2
                     /ApEinfo_fwdcolor=#ff47f2
     misc_feature    12338..12360
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#8f25ff
                     /ApEinfo_fwdcolor=#8f25ff
     CDS             5045..5405
                     /label="lacZα"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        12394..12413
                     /label="T3_promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      11629..12026
                     /label="tGPM1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(5774..6577)
                     /label="URA3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(4998..5018)
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     12395..12414
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     12357..12377
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2952..2969)
                     /label="ORF frame 3"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(11704..11732)
                     /label="498_GPM1t_RevSeqPrime"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    7063..7566
                     /label="CEN/ARS"
                     /ApEinfo_revcolor=#454f59
                     /ApEinfo_fwdcolor=#454f59
     misc_feature    3039..3059
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4657..4681
                     /label="attB2"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    10873..10898
                     /label="497_GPM1_FwdSeqPrime"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        9373..9784
                     /label="pRPR1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(12430..12468)
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#8f25ff
                     /ApEinfo_fwdcolor=#8f25ff
     CDS             complement(2973..4265)
                     /label="ORF frame 3"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    12430..12836
                     /label="TEF Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_binding    12329..12351
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    9700..9784
                     /label="RNAse P leader sequence"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3060..3245
                     /label="VP64"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4715..4731)
                     /label="pBluescriptKS_primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 CCGGATACAT TGACGGCGGA GCAAGCCAGG AGGAATTTTA CAAATTTATT AAGCCCATCT
       61 TGGAAAAAAT GGACGGCACC GAGGAGCTGC TGGTAAAGCT TAACAGAGAA GATCTGTTGC
      121 GCAAACAGCG CACTTTCGAC AATGGAAGCA TCCCCCACCA GATTCACCTG GGCGAACTGC
      181 ACGCTATCCT CAGGCGGCAA GAGGATTTCT ACCCCTTTTT GAAAGATAAC AGGGAAAAGA
      241 TTGAGAAAAT CCTCACATTT CGGATACCCT ACTATGTAGG CCCCCTCGCC CGGGGAAATT
      301 CCAGATTCGC GTGGATGACT CGCAAATCAG AAGAGACCAT CACTCCCTGG AACTTCGAGG
      361 AAGTCGTGGA TAAGGGGGCC TCTGCCCAGT CCTTCATCGA AAGGATGACT AACTTTGATA
      421 AAAATCTGCC TAACGAAAAG GTGCTTCCTA AACACTCTCT GCTGTACGAG TACTTCACAG
      481 TTTATAACGA GCTCACCAAG GTCAAATACG TCACAGAAGG GATGAGAAAG CCAGCATTCC
      541 TGTCTGGAGA GCAGAAGAAA GCTATCGTGG ACCTCCTCTT CAAGACGAAC CGGAAAGTTA
      601 CCGTGAAACA GCTCAAAGAA GACTATTTCA AAAAGATTGA ATGTTTCGAC TCTGTTGAAA
      661 TCAGCGGAGT GGAGGATCGC TTCAACGCAT CCCTGGGAAC GTATCACGAT CTCCTGAAAA
      721 TCATTAAAGA CAAGGACTTC CTGGACAATG AGGAGAACGA GGACATTCTT GAGGACATTG
      781 TCCTCACCCT TACGTTGTTT GAAGATAGGG AGATGATTGA AGAACGCTTG AAAACTTACG
      841 CTCATCTCTT CGACGACAAA GTCATGAAAC AGCTCAAGAG GCGCCGATAT ACAGGATGGG
      901 GGCGGCTGTC AAGAAAACTG ATCAATGGGA TCCGAGACAA GCAGAGTGGA AAGACAATCC
      961 TGGATTTTCT TAAGTCCGAT GGATTTGCCA ACCGGAACTT CATGCAGTTG ATCCATGATG
     1021 ACTCTCTCAC CTTTAAGGAG GACATCCAGA AAGCACAAGT TTCTGGCCAG GGGGACAGTC
     1081 TTCACGAGCA CATCGCTAAT CTTGCAGGTA GCCCAGCTAT CAAAAAGGGA ATACTGCAGA
     1141 CCGTTAAGGT CGTGGATGAA CTCGTCAAAG TAATGGGAAG GCATAAGCCC GAGAATATCG
     1201 TTATCGAGAT GGCCCGAGAG AACCAAACTA CCCAGAAGGG ACAGAAGAAC AGTAGGGAAA
     1261 GGATGAAGAG GATTGAAGAG GGTATAAAAG AACTGGGGTC CCAAATCCTT AAGGAACACC
     1321 CAGTTGAAAA CACCCAGCTT CAGAATGAGA AGCTCTACCT GTACTACCTG CAGAACGGCA
     1381 GGGACATGTA CGTGGATCAG GAACTGGACA TCAATCGGCT CTCCGACTAC GACGTGGATG
     1441 CTATCGTGCC CCAGTCTTTT CTCAAAGATG ATTCTATTGA TAATAAAGTG TTGACAAGAT
     1501 CCGATAAAAA TAGAGGGAAG AGTGATAACG TCCCCTCAGA AGAAGTTGTC AAGAAAATGA
     1561 AAAATTATTG GCGGCAGCTG CTGAACGCCA AACTGATCAC ACAACGGAAG TTCGATAATC
     1621 TGACTAAGGC TGAACGAGGT GGCCTGTCTG AGTTGGATAA AGCCGGCTTC ATCAAAAGGC
     1681 AGCTTGTTGA GACACGCCAG ATCACCAAGC ACGTGGCCCA AATTCTCGAT TCACGCATGA
     1741 ACACCAAGTA CGATGAAAAT GACAAACTGA TTCGAGAGGT GAAAGTTATT ACTCTGAAGT
     1801 CTAAGCTGGT CTCAGATTTC AGAAAGGACT TTCAGTTTTA TAAGGTGAGA GAGATCAACA
     1861 ATTACCACCA TGCGCATGAT GCCTACCTGA ATGCAGTGGT AGGCACTGCA CTTATCAAAA
     1921 AATATCCCAA GCTTGAATCT GAATTTGTTT ACGGAGACTA TAAAGTGTAC GATGTTAGGA
     1981 AAATGATCGC AAAGTCTGAG CAGGAAATAG GCAAGGCCAC CGCTAAGTAC TTCTTTTACA
     2041 GCAATATTAT GAATTTTTTC AAGACCGAGA TTACACTGGC CAATGGAGAG ATTCGGAAGC
     2101 GACCACTTAT CGAAACAAAC GGAGAAACAG GAGAAATCGT GTGGGACAAG GGTAGGGATT
     2161 TCGCGACAGT CCGGAAGGTC CTGTCCATGC CGCAGGTGAA CATCGTTAAA AAGACCGAAG
     2221 TACAGACCGG AGGCTTCTCC AAGGAAAGTA TCCTCCCGAA AAGGAACAGC GACAAGCTGA
     2281 TCGCACGCAA AAAAGATTGG GACCCCAAGA AATACGGCGG ATTCGATTCT CCTACAGTCG
     2341 CTTACAGTGT ACTGGTTGTG GCCAAAGTGG AGAAAGGGAA GTCTAAAAAA CTCAAAAGCG
     2401 TCAAGGAACT GCTGGGCATC ACAATCATGG AGCGATCAAG CTTCGAAAAA AACCCCATCG
     2461 ACTTTCTCGA GGCGAAAGGA TATAAAGAGG TCAAAAAAGA CCTCATCATT AAGCTTCCCA
     2521 AGTACTCTCT CTTTGAGCTT GAAAACGGCC GGAAACGAAT GCTCGCTAGT GCGGGCGAGC
     2581 TGCAGAAAGG TAACGAGCTG GCACTGCCCT CTAAATACGT TAATTTCTTG TATCTGGCCA
     2641 GCCACTATGA AAAGCTCAAA GGGTCTCCCG AAGATAATGA GCAGAAGCAG CTGTTCGTGG
     2701 AACAACACAA ACACTACCTT GATGAGATCA TCGAGCAAAT AAGCGAATTC TCCAAAAGAG
     2761 TGATCCTCGC CGACGCTAAC CTCGATAAGG TGCTTTCTGC TTACAATAAG CACAGGGATA
     2821 AGCCCATCAG GGAGCAGGCA GAAAACATTA TCCACTTGTT TACTCTGACC AACTTGGGCG
     2881 CGCCTGCAGC CTTCAAGTAC TTCGACACCA CCATAGACAG AAAGCGGTAC ACCTCTACAA
     2941 AGGAGGTCCT GGACGCCACA CTGATTCATC AGTCAATTAC GGGGCTCTAT GAAACAAGAA
     3001 TCGACCTCTC TCAGCTCGGT GGAGACAGCA GGGCTGACCC CAAGAAGAAG AGGAAGGTGG
     3061 AGGCCAGCGG TTCCGGACGG GCTGACGCAT TGGACGATTT TGATCTGGAT ATGCTGGGAA
     3121 GTGACGCCCT CGATGATTTT GACCTTGACA TGCTTGGTTC GGATGCCCTT GATGACTTTG
     3181 ACCTCGACAT GCTCGGCAGT GACGCCCTTG ATGATTTCGA CCTGGACATG CTGATTAACT
     3241 CTAGAAGTTC CGGATCTCCG AAAAAGAAAC GCAAAGTTGG TAGCCAGTAC CTGCCCGACA
     3301 CCGACGACCG GCACCGGATC GAGGAAAAGC GGAAGCGGAC CTACGAGACA TTCAAGAGCA
     3361 TCATGAAGAA GTCCCCCTTC AGCGGCCCCA CCGACCCTAG ACCTCCACCT AGAAGAATCG
     3421 CCGTGCCCAG CAGATCCAGC GCCAGCGTGC CAAAACCTGC CCCCCAGCCT TACCCCTTCA
     3481 CCAGCAGCCT GAGCACCATC AACTACGACG AGTTCCCTAC CATGGTGTTC CCCAGCGGCC
     3541 AGATCTCTCA GGCCTCTGCT CTGGCTCCAG CCCCTCCTCA GGTGCTGCCT CAGGCTCCTG
     3601 CTCCTGCACC AGCTCCAGCC ATGGTGTCTG CACTGGCTCA GGCACCAGCA CCCGTGCCTG
     3661 TGCTGGCTCC TGGACCTCCA CAGGCTGTGG CTCCACCAGC CCCTAAACCT ACACAGGCCG
     3721 GCGAGGGCAC ACTGTCTGAA GCTCTGCTGC AGCTGCAGTT CGACGACGAG GATCTGGGAG
     3781 CCCTGCTGGG AAACAGCACC GATCCTGCCG TGTTCACCGA CCTGGCCAGC GTGGACAACA
     3841 GCGAGTTCCA GCAGCTGCTG AACCAGGGCA TCCCTGTGGC CCCTCACACC ACCGAGCCCA
     3901 TGCTGATGGA ATACCCCGAG GCCATCACCC GGCTCGTGAC AGGCGCTCAG AGGCCTCCTG
     3961 ATCCAGCTCC TGCCCCTCTG GGAGCACCAG GCCTGCCTAA TGGACTGCTG TCTGGCGACG
     4021 AGGACTTCAG CTCTATCGCC GACATGGACT TCTCCGCACT GCTGGGTAGC GGATCGGGAT
     4081 CTCGGGATTC CAGGGAAGGG ATGTTTTTGC CGAAGCCTGA GGCCGGCTCC GCTATTAGTG
     4141 ACGTGTTTGA GGGCCGCGAG GTGTGCCAGC CAAAACGAAT CCGGCCATTT CATCCTCCAG
     4201 GAAGTCCATG GGCCAACCGC CCACTCCCCG CCAGCCTCGC ACCAACACCA ACCGGTCCAG
     4261 TACATGAGCC AGTCGGGTCA CTGACCCCGG CACCAGTCCC TCAGCCACTG GATCCAGCGC
     4321 CCGCAGTGAC TCCCGAGGCC AGTCACCTGT TGGAGGATCC CGATGAAGAG ACGAGCCAGG
     4381 CTGTCAAAGC CCTTCGGGAG ATGGCCGATA CTGTGATTCC CCAGAAGGAA GAGGCTGCAA
     4441 TCTGTGGCCA AATGGACCTT TCCCATCCGC CCCCAAGGGG CCATCTGGAT GAGCTGACAA
     4501 CCACACTTGA GTCCATGACC GAGGATCTGA ACCTGGACTC ACCCCTGACC CCGGAATTGA
     4561 ACGAGATTCT GGATACCTTC CTGAACGACG AGTGCCTCTT GCATGCCATG CATATCAGCA
     4621 CAGGACTGTC CATCTTCGAC ACATCTCTGT TTTGAaaccc agctttcttg tacaaagtgg
     4681 tgatgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg aGTCATGTAA
     4741 TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT CCGCTCTAAC CGAAAAGGAA
     4801 GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTATAGTTAT GTTAGTATTA
     4861 AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT ACAGACGCGT GTACGCATGT
     4921 AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC GCTCGAAGGC TTTAATTTGC
     4981 GGCCGGTACc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5041 tacaACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC
     5101 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT
     5161 TGCGCAGCCT GAATGGCGAA TGGCGCGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
     5221 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
     5281 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
     5341 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
     5401 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA
     5461 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC
     5521 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA
     5581 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA
     5641 TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATAGGGTA
     5701 ATAACTGATA TAATTAAATT GAAGCTCTAA TTTGTGAGTT TAGTATACAT GCATTTACTT
     5761 ATAATACAGT TTTTTAGTTT TGCTGGCCGC ATCTTCTCAA ATATGCTTCC CAGCCTGCTT
     5821 TTCTGTAACG TTCACCCTCT ACCTTAGCAT CCCTTCCCTT TGCAAATAGT CCTCTTCCAA
     5881 CAATAATAAT GTCAGATCCT GTAGAGACCA CATCATCCAC GGTTCTATAC TGTTGACCCA
     5941 ATGCGTCTCC CTTGTCATCT AAACCCACAC CGGGTGTCAT AATCAACCAA TCGTAACCTT
     6001 CATCTCTTCC ACCCATGTCT CTTTGAGCAA TAAAGCCGAT AACAAAATCT TTGTCGCTCT
     6061 TCGCAATGTC AACAGTACCC TTAGTATATT CTCCAGTAGA TAGGGAGCCC TTGCATGACA
     6121 ATTCTGCTAA CATCAAAAGG CCTCTAGGTT CCTTTGTTAC TTCTTCTGCC GCCTGCTTCA
     6181 AACCGCTAAC AATACCTGGG CCCACCACAC CGTGTGCATT CGTAATGTCT GCCCATTCTG
     6241 CTATTCTGTA TACACCCGCA GAGTACTGCA ATTTGACTGT ATTACCAATG TCAGCAAATT
     6301 TTCTGTCTTC GAAGAGTAAA AAATTGTACT TGGCGGATAA TGCCTTTAGC GGCTTAACTG
     6361 TGCCCTCCAT GGAAAAATCA GTCAAGATAT CCACATGTGT TTTTAGTAAA CAAATTTTGG
     6421 GACCTAATGC TTCAACTAAC TCCAGTAATT CCTTGGTGGT ACGAACATCC AATGAAGCAC
     6481 ACAAGTTTGT TTGCTTTTCG TGCATGATAT TAAATAGCTT GGCAGCAACA GGACTAGGAT
     6541 GAGTAGCAGC ACGTTCCTTA TATGTAGCTT TCGACATGAT TTATCTTCGT TTCCTGCAGG
     6601 TTTTTGTTCT GTGCAGTTGG GTTAAGAATA CTGGGCAATT TCATGTTTCT TCAACACTAC
     6661 ATATGCGTAT ATATACCAAT CTAAGTCTGT GCTCCTTCCT TCGTTCTTCC TTCTGTTCGG
     6721 AGATTACCGA ATCAAAAAAA TTTCAAAGAA ACCGAAATCA AAAAAAAGAA TAAAAAAAAA
     6781 ATGATGAATT GAATTGAAAA GCTGTGGTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
     6841 CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT
     6901 GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC
     6961 AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT
     7021 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGGA CGGATCGCTT GCCTGTAACT
     7081 TACACGCGCC TCGTATCTTT TAATGATGGA ATAATTTGGG AATTTACTCT GTGTTTATTT
     7141 ATTTTTATGT TTTGTATTTG GATTTTAGAA AGTAAATAAA GAAGGTAGAA GAGTTACGGA
     7201 ATGAAGAAAA AAAAATAAAC AAAGGTTTAA AAAATTTCAA CAAAAAGCGT ACTTTACATA
     7261 TATATTTATT AGACAAGAAA AGCAGATTAA ATAGATATAC ATTCGATTAA CGATAAGTAA
     7321 AATGTAAAAT CACAGGATTT TCGTGTGTGG TCTTCTACAC AGACAAGATG AAACAATTCG
     7381 GCATTAATAC CTGAGAGCAG GAAGAGCAAG ATAAAAGGTA GTATTTGTTG GCGATCCCCC
     7441 TAGAGTCTTT TACATCTTCG GAAAACAAAA ACTATTTTTT CTTTAATTTC TTTTTTTACT
     7501 TTCTATTTTT AATTTATATA TTTATATTAA AAAATTTAAA TTATAATTAT TTTTATAGCA
     7561 CGTGATcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7621 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7681 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7741 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7801 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7861 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7921 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7981 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8041 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8101 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8161 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8221 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8281 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8341 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8401 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8461 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8521 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8581 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8641 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9361 ggccttttgc tcgggggatc tgccaattga acataacatg gtagttacat atactagtaa
     9421 tatggttcgg cacacattaa aagtataaaa actatctgaa ttacgaatta catatattgg
     9481 tcataaaaat caatcaatca tcgtgtgttt tatatgtctc ttatctaagt ataagaatat
     9541 ccatagttaa tattcactta cgctaccttt taacctgtaa tcattgtcaa caggatatgt
     9601 taacgaccca cattgataaa cgctagtatt tctttttcct cttcttattg gccggctgtc
     9661 tctatactcc cctatagtct gtttcttttc gtttcgattg tccctatcag tgatagagat
     9721 ggcgcacatg gtacgctgtg gtgctcgcgg ctgggaacga aactctggga gctgcgattg
     9781 gcagCGGCCG Cccatatcca acttccaatt taatctttct tttttaattt tcacttattt
     9841 gcgatacaga aagaaaaaag cgatagtaac tattgaattt tgtttggatt tggttagatt
     9901 agatatggtt tctctttata tttacatgct aaaaatgggc tacaccagag atacataatt
     9961 agatatatat acgccagtac accttatcgg cccaagcctt gtcccaaggc agcgttttgt
    10021 tcttggaaac gctgccctac acgttcgcta tgcttcaaga acttttctga gcacttcatg
    10081 atgcatgttt gttccttatt ggttagcttt gatgttgtga agtcattgac acagtctgtg
    10141 aaacatcttt ctaccagatt agagtacaaa cgcatgaaat ccttcatttg cttttgttcc
    10201 actacttttt ggaactcttg ttgttctttC CTGCAGGtag tcgtgcaatg tatgacttta
    10261 agatttgtga gcaggaagaa aagggagaat cttctaacga taaacccttg aaaaactggg
    10321 tagactacgc tatgttgagt tgctacgcag gctgcacaat tacacgagaa tgctcccgcc
    10381 taggatttaa ggctaaggga cgtgcaatgc agacgacaga tctaaatgac cgtgtcggtg
    10441 aagtgttcgc caaacttttc ggttaacaca tgcagtgatg cacgcgcgat ggtgctaagt
    10501 tacatatata tatatatata tatatatata tatatatagc catagtgatg tctaagtaac
    10561 ctttatggta tatttcttaa tgtggaaaga tactagcgcg cgcacccaca cacaagcttc
    10621 gtcttttctt gaagaaaaga ggaagctcgc taaatgggat tccactttcc gttccctgcc
    10681 agctgatgga aaaaggttag tggaacgatg aagaataaaa agagagatcc actgaggtga
    10741 aatttcagct gacagcgagt ttcatgatcg tgatgaacaa tggtaacgag ttgtggctgt
    10801 tgccagggag ggtggttctc aacttttaat gtatggccaa atcgctactt gggtttgtta
    10861 tataacaaag aagaaataat gaactgattc tcttcctcct tcttgtcctt tcttaattct
    10921 gttgtaatta ccttcctttg taattttttt tgtaattatt cttcttaata atccaaacaa
    10981 acacacatat tacaatatat taaaatgtct agattagata aaagtaaagt gattaacagc
    11041 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
    11101 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
    11161 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
    11221 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
    11281 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
    11341 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
    11401 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
    11461 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
    11521 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
    11581 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaagt ctgaagaatg
    11641 aatgatttga tgatttcttt ttccctccat ttttcttact gaatatatca atgatataga
    11701 cttgtatagt ttattatttc aaattaagta gctatatata gtcaagataa cgtttgtttg
    11761 acacgattac attattcgtc gacatctttt ttcagcctgt cgtggtagca atttgaggag
    11821 tattattaat tgaataggtt cattttgcgc tcgcataaac agttttcgtc agggacagta
    11881 tgttggaatg agtggtaatt aatggtgaca tgacatgtta tagcaataac cttgatgttt
    11941 acatcgtagt ttaatgtaca ccccgcgaat tcgttcaagt aggagtgcac caattgcaaa
    12001 gggaaaagct gaatgggcag ttcgaaCCGC GGtctttcct gcgttatccc ctgattctgt
    12061 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    12121 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    12181 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    12241 cagtgagcgc aacgcaatta atgtgagtta cctcactcat taggcacccc aggctttaca
    12301 ctttatgctt ccggctccta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    12361 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
    12421 gctggAGCTC ATAGCTTCAA AATGTTTCTA CTCCTTTTTT ACTCTTCCAG ATTTTCTCGG
    12481 ACTCCGCGCA TCGCCGTACC ACTTCAAAAC ACCCAAGCAC AGCATACTAA ATTTCCCCTC
    12541 TTTCTTCCTC TAGGGTGTCG TTAATTACCC GTACTAAAGG TTTGGAAAAG AAAAAAGAGA
    12601 CCGCCTCGTT TCTTTTTCTT CGTCGAAAAA GGCAATAAAA ATTTTTATCA CGTTTCTTTT
    12661 TCTTGAAAAT TTTTTTTTTG ATTTTTTTCT CTTTCGATGA CCTCCCATTG ATATTTAAGT
    12721 TAATAAACGG TCTTCAATTT CTCAAGTTTC AGTTTCATTT TTCTTGTTCT ATTACAACTT
    12781 TTTTTACTTC TTGCTCATTA GAAAGAAAGC ATAGCAATCT AATCTAAGTT TTCTAGAGat
    12841 gtctagagcc ccaaagaaga agagaaaagt tagacccggg ATGGACAAGA AGTACTCCAT
    12901 TGGGCTCGCT ATCGGCACAA ACAGCGTCGG CTGGGCCGTC ATTACGGACG AGTACAAGGT
    12961 GCCGAGCAAA AAATTCAAAG TTCTGGGCAA TACCGATCGC CACAGCATAA AGAAGAACCT
    13021 CATTGGCGCC CTCCTGTTCG ACTCCGGGGA GACGGCCGAA GCCACGCGGC TCAAAAGAAC
    13081 AGCACGGCGC AGATATACCC GCAGAAAGAA TCGGATCTGC TACCTGCAGG AGATCTTTAG
    13141 TAATGAGATG GCTAAGGTGG ATGACTCTTT CTTCCATAGG CTGGAGGAGT CCTTTTTGGT
    13201 GGAGGAGGAT AAAAAGCACG AGCGCCACCC AATCTTTGGC AATATCGTGG ACGAGGTGGC
    13261 GTACCATGAA AAGTACCCAA CCATATATCA TCTGAGGAAG AAGCTTGTAG ACAGTACTGA
    13321 TAAGGCTGAC TTGCGGTTGA TCTATCTCGC GCTGGCGCAT ATGATCAAAT TTCGGGGACA
    13381 CTTCCTCATC GAGGGGGACC TGAACCCAGA CAACAGCGAT GTCGACAAAC TCTTTATCCA
    13441 ACTGGTTCAG ACTTACAATC AGCTTTTCGA AGAGAACCCG ATCAACGCAT CCGGAGTTGA
    13501 CGCCAAAGCA ATCCTGAGCG CTAGGCTGTC CAAATCCCGG CGGCTCGAAA ACCTCATCGC
    13561 ACAGCTCCCT GGGGAGAAGA AGAACGGCCT GTTTGGTAAT CTTATCGCCC TGTCACTCGG
    13621 GCTGACCCCC AACTTTAAAT CTAACTTCGA CCTGGCCGAA GATGCCAAGC TTCAACTGAG
    13681 CAAAGACACC TACGATGATG ATCTCGACAA TCTGCTGGCC CAGATCGGCG ACCAGTACGC
    13741 AGACCTTTTT TTGGCGGCAA AGAACCTGTC AGACGCCATT CTGCTGAGTG ATATTCTGCG
    13801 AGTGAACACG GAGATCACCA AAGCTCCGCT GAGCGCTAGT ATGATCAAGC GCTATGATGA
    13861 GCACCACCAA GACTTGACTT TGCTGAAGGC CCTTGTCAGA CAGCAACTGC CTGAGAAGTA
    13921 CAAGGAAATT TTCTTCGATC AGTCTAAAAA TGGCTACG
//