LOCUS pC0043_PspCas13b_crRNA_ 2962 bp ds-DNA circular 18-OCT-2017 DEFINITION . FEATURES Location/Qualifiers -10_signal 1652..1657 /label="bla promoter (counter-clockwise); GAGACA" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 -35_signal complement(2909..2914) /label="RNAI promoter (clockwise); TTGAAG" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 745..749 /label="RBS" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 1038..1043 /label="BpiI" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac -35_signal 1631..1636 /label="bla promoter (counter-clockwise); TTCAAA" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 1701..2561 /label="bla" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 sig_peptide 1701..1769 /label="bla" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 -10_signal 2753..2758 /label="RNAII promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 756..758 /label="Start codon" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 source complement(1501..1500) /label="pUC19" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 1046..1081 /label="PspCas13b DR" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1775..1804 /label="negative control spacer" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc -10_signal complement(2887..2892) /label="RNAI promoter (clockwise); GCTACA" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature complement(1968..1994) /label="New AGAAW target 2" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature complement(1025..1030) /label="BpiI" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 1826..1855 /label="" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 rep_origin 2732..358 /label="pMB1 origin of replication" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_RNA complement(2771..2878) /label="RNAI transcript" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1082..1087 /label="U6 terminator" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 -35_signal 2732..2737 /label="RNAII promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 774..1022 /label="Human U6 Promoter" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_RNA 2768..358 /label="RNAII transcript (complementary strand)" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 ORIGIN 1 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 61 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 481 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 541 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 601 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 661 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 721 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccagagggcc 781 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg 841 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat 901 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta 961 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca 1021 ccATGTCTTC CTGGGACGAA GACAAgttgt ggaaggtcca gttttgaggg gctattacaa 1081 cTTTTTTggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 1141 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 1201 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 1261 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 1321 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1381 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1441 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1501 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1561 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1621 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1681 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1741 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1801 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1861 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 1921 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 1981 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2041 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2101 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2161 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2221 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2281 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2341 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2401 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2461 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2521 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2581 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2641 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2701 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2761 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2821 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 2881 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 2941 tcgctctgct aatcctgtta cc //