LOCUS       pC0051_W85X_REPAIR_targ 2990 bp ds-DNA     circular     18-OCT-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        774..1022
                     /label="U6"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    505..534
                     /label="Gibson homology"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1074..1109
                     /label="PspCas13b DR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1040..1040
                     /label="C mismatch"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    745..749
                     /label="RBS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     -35_signal      2760..2765
                     /label="RNAII promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1854..1883
                     /label=""
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     sig_peptide     1729..1797
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1023..1073
                     /label="W58X tageting guide"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     -35_signal      complement(2937..2942)
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     -10_signal      2781..2786
                     /label="RNAII promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     -35_signal      1659..1664
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1803..1832
                     /label="negative control spacer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1110..1115
                     /label="U6 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     -10_signal      1680..1685
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1729..2589
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     -10_signal      complement(2915..2920)
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_RNA        complement(2799..2906)
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      2760..358
                     /label="pMB1 origin of replication"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_RNA        2796..358
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1996..2022)
                     /label="New AGAAW target 2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    756..758
                     /label="Start codon"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
       61 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      481 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      541 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      601 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      661 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      721 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccagagggcc
      781 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
      841 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
      901 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
      961 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     1021 ccgatagaat gttctaaacC atcctgcggc ctctactctg cattcaatta catgttgtgg
     1081 aaggtccagt tttgaggggc tattacaacT TTTTTggtac cgagctcgaa ttcactggcc
     1141 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1201 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1261 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     1321 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     1381 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     1441 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     1501 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     1561 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1741 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1801 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1861 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1921 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1981 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2041 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2101 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2161 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2221 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2281 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2341 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2401 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2461 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2521 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2581 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2701 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2761 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2821 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2881 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2941 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
//