LOCUS       pC0052_REPAIR_non-targe 2969 bp ds-DNA     circular     18-OCT-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     -10_signal      complement(2894..2899)
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1833..1862
                     /label=""
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_RNA        complement(2778..2885)
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1023..1052
                     /label="non-targeting guide"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1782..1811
                     /label="negative control spacer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    745..749
                     /label="RBS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     sig_peptide     1708..1776
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        774..1022
                     /label="U6"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     -10_signal      2760..2765
                     /label="RNAII promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1089..1094
                     /label="U6 terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1053..1088
                     /label="PspCas13b DR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     -35_signal      1638..1643
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1975..2001)
                     /label="New AGAAW target 2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      2739..358
                     /label="pMB1 origin of replication"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1096..1125
                     /label="Gibson homology KpnI"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     -35_signal      2739..2744
                     /label="RNAII promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     -10_signal      1659..1664
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1708..2568
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    756..758
                     /label="Start codon"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     -35_signal      complement(2916..2921)
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(992..1022)
                     /label="GGTGTTTCGTCCTTTCCACAAGATATATAAA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_RNA        2775..358
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
       61 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      481 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      541 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      601 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      661 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      721 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccagagggcc
      781 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
      841 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
      901 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
      961 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     1021 ccGTAATGCC TGGCTTGTCG ACGCATAGTC TGgttgtgga aggtccagtt ttgaggggct
     1081 attacaacTT TTTTggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
     1141 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1201 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1261 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1321 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     1381 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     1441 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     1501 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1561 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1621 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1681 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1741 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1801 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1861 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1921 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1981 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2041 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2101 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2161 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2221 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2281 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2341 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2401 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2461 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2521 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2581 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2641 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2701 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2761 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2821 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2881 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2941 acatacctcg ctctgctaat cctgttacc
//