LOCUS       pC0055_CMV-dPspCas13b-G 9525 bp ds-DNA     circular     18-OCT-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     polyA_site      5125..5349
                     /label="BGH pA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        5828..6197
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      5395..5823
                     /label="f1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(7714..8384)
                     /label="pUC origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4094..4096
                     /label="T375G"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1304..1321
                     /label="HEPN1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    758..776
                     /label="CGCCCCATTGACGCAAATG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        232..819
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(5075..5082)
                     /label="NotI"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(8529..9389)
                     /label="Amp(R)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4433..4435
                     /label="E to Q muatnt"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        863..879
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3917..5074
                     /label="huADAR2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    769..789
                     /label="CMV forward primer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    923..3871
                     /label="dPspCas13b delta 984-1090"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    758..776
                     /label="CMV For"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(9390..9488)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3878..3910
                     /label="HIV NES"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     polyA_signal    7201..7331
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    863..882
                     /label="T7 primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(5119..5136)
                     /label="BGH reverse primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5089..5092
                     /label="Golden Gate"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             6233..7027
                     /label="Neo(R)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(5081..5110)
                     /label="NotI GH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    822..846
                     /label="TGGCTAACTAGAGAACCCACTGCTT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(5161..5179)
                     /label="AAGGAAGGCACGGGGGAGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgaacat ccccgctctg gtggaaaacc agaagaagta
      961 ctttggcacc tacagcgtga tggccatgct gaacgctcag accgtgctgg accacatcca
     1021 gaaggtggcc gatattgagg gcgagcagaa cgagaacaac gagaatctgt ggtttcaccc
     1081 cgtgatgagc cacctgtaca acgccaagaa cggctacgac aagcagcccg agaaaaccat
     1141 gttcatcatc gagcggctgc agagctactt cccattcctg aagatcatgg ccgagaacca
     1201 gagagagtac agcaacggca agtacaagca gaaccgcgtg gaagtgaaca gcaacgacat
     1261 cttcgaggtg ctgaagcgcg ccttcggcgt gctgaagatg tacagggacc tgaccaacGC
     1321 Atacaagacc tacgaggaaa agctgaacga cggctgcgag ttcctgacca gcacagagca
     1381 acctctgagc ggcatgatca acaactacta cacagtggcc ctgcggaaca tgaacgagag
     1441 atacggctac aagacagagg acctggcctt catccaggac aagcggttca agttcgtgaa
     1501 ggacgcctac ggcaagaaaa agtcccaagt gaataccgga ttcttcctga gcctgcagga
     1561 ctacaacggc gacacacaga agaagctgca cctgagcgga gtgggaatcg ccctgctgat
     1621 ctgcctgttc ctggacaagc agtacatcaa catctttctg agcaggctgc ccatcttctc
     1681 cagctacaat gcccagagcg aggaacggcg gatcatcatc agatccttcg gcatcaacag
     1741 catcaagctg cccaaggacc ggatccacag cgagaagtcc aacaagagcg tggccatgga
     1801 tatgctcaac gaagtgaagc ggtgccccga cgagctgttc acaacactgt ctgccgagaa
     1861 gcagtcccgg ttcagaatca tcagcgacga ccacaatgaa gtgctgatga agcggagcag
     1921 cgacagattc gtgcctctgc tgctgcagta tatcgattac ggcaagctgt tcgaccacat
     1981 caggttccac gtgaacatgg gcaagctgag atacctgctg aaggccgaca agacctgcat
     2041 cgacggccag accagagtca gagtgatcga gcagcccctg aacggcttcg gcagactgga
     2101 agaggccgag acaatgcgga agcaagagaa cggcaccttc ggcaacagcg gcatccggat
     2161 cagagacttc gagaacatga agcgggacga cgccaatcct gccaactatc cctacatcgt
     2221 ggacacctac acacactaca tcctggaaaa caacaaggtc gagatgttta tcaacgacaa
     2281 agaggacagc gccccactgc tgcccgtgat cgaggatgat agatacgtgg tcaagacaat
     2341 ccccagctgc cggatgagca ccctggaaat tccagccatg gccttccaca tgtttctgtt
     2401 cggcagcaag aaaaccgaga agctgatcgt ggacgtgcac aaccggtaca agagactgtt
     2461 ccaggccatg cagaaagaag aagtgaccgc cgagaatatc gccagcttcg gaatcgccga
     2521 gagcgacctg cctcagaaga tcctggatct gatcagcggc aatgcccacg gcaaggatgt
     2581 ggacgccttc atcagactga ccgtggacga catgctgacc gacaccgagc ggagaatcaa
     2641 gagattcaag gacgaccgga agtccattcg gagcgccgac aacaagatgg gaaagagagg
     2701 cttcaagcag atctccacag gcaagctggc cgacttcctg gccaaggaca tcgtgctgtt
     2761 tcagcccagc gtgaacgatg gcgagaacaa gatcaccggc ctgaactacc ggatcatgca
     2821 gagcgccatt gccgtgtacg atagcggcga cgattacgag gccaagcagc agttcaagct
     2881 gatgttcgag aaggcccggc tgatcggcaa gggcacaaca gagcctcatc catttctgta
     2941 caaggtgttc gcccgcagca tccccgccaa tgccgtcgag ttctacgagc gctacctgat
     3001 cgagcggaag ttctacctga ccggcctgtc caacgagatc aagaaaggca acagagtgga
     3061 tgtgcccttc atccggcggg accagaacaa gtggaaaaca cccgccatga agaccctggg
     3121 cagaatctac agcgaggatc tgcccgtgga actgcccaga cagatgttcg acaatgagat
     3181 caagtcccac ctgaagtccc tgccacagat ggaaggcatc gacttcaaca atgccaacgt
     3241 gacctatctg atcgccgagt acatgaagag agtgctggac gacgacttcc agaccttcta
     3301 ccagtggaac cgcaactacc ggtacatgga catgcttaag ggcgagtacg acagaaaggg
     3361 ctccctgcag cactgcttca ccagcgtgga agagagagaa ggcctctgga aagagcgggc
     3421 ctccagaaca gagcggtaca gaaagcaggc cagcaacaag atccgcagca accggcagat
     3481 gagaaacgcc agcagcgaag agatcgagac aatcctggat aagcggctga gcaacagccg
     3541 gaacgagtac cagaaaagcg agaaagtgat ccggcgctac agagtgcagg atgccctgct
     3601 gtttctgctg gccaaaaaga ccctgaccga actggccgat ttcgacggcg agaggttcaa
     3661 actgaaagaa atcatgcccg acgccgagaa gggaatcctg agcgagatca tgcccatgag
     3721 cttcaccttc gagaaaggcg gcaagaagta caccatcacc agcgagggca tgaagctgaa
     3781 gaactacggc gacttctttg tgctggctag cgacaagagg atcggcaacc tgctggaact
     3841 cgtgggcagc gacatcgtgt ccaaagagga tggatccCTT CAACTGCCTC CACTTGAAAG
     3901 ACTGACACTG GGATCCcagc tgcatttacc gcaggtttta gctgacgctg tctcacgcct
     3961 ggtcctgggt aagtttggtg acctgaccga caacttctcc tcccctcacg ctcgcagaaa
     4021 agtgctggct ggagtcgtca tgacaacagg cacagatgtt aaagatgcca aggtgataag
     4081 tgtttctaca ggaGGCaaat gtattaatgg tgaatacatg agtgatcgtg gccttgcatt
     4141 aaatgactgc catgcagaaa taatatctcg gagatccttg ctcagatttc tttatacaca
     4201 acttgagctt tacttaaata acaaagatga tcaaaaaaga tccatctttc agaaatcaga
     4261 gcgagggggg tttaggctga aggagaatgt ccagtttcat ctgtacatca gcacctctcc
     4321 ctgtggagat gccagaatct tctcaccaca tgagccaatc ctggaagaac cagcagatag
     4381 acacccaaat cgtaaagcaa gaggacagct acggaccaaa atagagtctg gtCaggggac
     4441 gattccagtg cgctccaatg cgagcatcca aacgtgggac ggggtgctgc aaggggagcg
     4501 gctgctcacc atgtcctgca gtgacaagat tgcacgctgg aacgtggtgg gcatccaggg
     4561 atcActgctc agcattttcg tggagcccat ttacttctcg agcatcatcc tgggcagcct
     4621 ttaccacggg gaccaccttt ccagggccat gtaccagcgg atctccaaca tagaggacct
     4681 gccacctctc tacaccctca acaagccttt gctcagtggc atcagcaatg cagaagcacg
     4741 gcagccaggg aaggccccca acttcagtgt caactggacg gtaggcgact ccgctattga
     4801 ggtcatcaac gccacgactg ggaaggatga gctgggccgc gcgtcccgcc tgtgtaagca
     4861 cgcgttgtac tgtcgctgga tgcgtgtgca cggcaaggtt ccctcccact tactacgctc
     4921 caagattacc aagcccaacg tgtaccatga gtccaagctg gcggcaaagg agtaccaggc
     4981 cgccaaggcg cgtctgttca cagccttcat caaggcgggg ctgggggcct gggtggagaa
     5041 gcccaccgag caggaccagt tctcactcac gTAAgcggcc gctcgagtct agagggcccg
     5101 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5161 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5221 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5281 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5341 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5401 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5461 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5521 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5581 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5641 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5701 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5761 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5821 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5881 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5941 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6001 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6061 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     6121 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6181 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6241 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6301 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6361 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6421 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6481 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6541 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6601 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6661 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6721 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6781 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6841 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6901 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6961 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7021 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     7081 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7141 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7201 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7261 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7321 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7561 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8281 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
     8341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8521 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8581 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8641 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8701 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8761 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8821 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8881 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8941 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9001 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9061 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9121 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9181 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9241 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9301 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9361 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9421 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9481 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//