LOCUS pEB1-GFPmut2 4391 bp DNA circular UNA 25-OCT-2017 DEFINITION pEB1 plasmid with GFPmut2. ACCESSION urn.local...3a-87y487g VERSION urn.local...3a-87y487g KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..144 /created_by="User" /modified_by="User" /label="proC promoter" motif 63..68 /created_by="User" /modified_by="User" /label="-35 Box" motif 86..91 /created_by="User" /label="-10 Box" motif 98 /created_by="User" /label="TransStart" misc_feature 145..170 /created_by="User" /modified_by="User" /label="UArbs" CDS 171..887 /created_by="User" /modified_by="User" /label="GFPmut2 (avGFP nt)" motif 363..365 /created_by="User" /label="S65A" motif 372..374 /created_by="User" /label="V68L" motif 384..386 /created_by="User" /label="S72A" misc_feature 446..451 /Recognition_pattern="T^GTACA" /annotation_group="TGTACA" /label="BsrGI" /note="Geneious type: restriction site" misc_feature 657..662 /Recognition_pattern="GTT^AAC" /annotation_group="GTTAAC" /label="HpaI" /note="Geneious type: restriction site" misc_feature 657..662 /Recognition_pattern="GTY^RAC" /annotation_group="GTYRAC" /label="HincII" /note="Geneious type: restriction site" motif 786..788 /created_by="User" /label="A206K" terminator 919..1017 /modified_by="User" /label="rrnB T1 terminator" rep_origin complement(1148..3248) /modified_by="User" /label="Origin of Rep. from pUA66" primer_bind complement(1444..1467) /Sequence="AAAGGGAAAACTGTCCATATGCAC" /Hairpin_Tm="39.7" /Tm="59.8" /Self_Dimer_Tm="None" /%GC="41.7" /created_by="primer3" /label="T1_from_mSC101_R02" CDS complement(1549..2499) /label="RepA" terminator complement(3260..3355) /modified_by="User" /label="λ T0 terminator" CDS complement(3386..4180) /modified_by="User" /label="KmR" promoter complement(4269..4317) /created_by="User" /label="KmR Promoter" primer_bind 4273..4293 /Sequence="AGGGCTTCCCAACCTTACCAG" /Hairpin_Tm="48.5" /Tm="61.7" /Self_Dimer_Tm="4.5" /%GC="57.1" /modified_by="User" /label="pZSeq2_Kan" primer_bind 4364..4383 /Sequence="GCGTATCACGAGGCCCTTTC" /Hairpin_Tm="46.1" /Tm="61.2" /Self_Dimer_Tm="None" /%GC="60.0" /modified_by="User" /label="pZ_seq_fwr" ORIGIN 1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata 61 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct 121 ctacaaataa ttttgtttaa cttttctaga tttaagaagg agatatacat atgagtaaag 181 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg 241 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc 301 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt 361 tcgcgtatgg tcttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt 421 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg 481 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 541 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca 601 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta 661 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac 721 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac 781 aatctaagct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 841 taacagctgc tgggattaca catggcatgg atgaactata caaataaatg tccagacctg 901 caggcatgca agctctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 961 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc 1021 tagacctagg gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt 1081 tatcagaatc gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag 1141 aattgccatg attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt 1201 gattcgataa gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca 1261 agttgtctca ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt 1321 ctattaggtg ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct 1381 ttgaatgcac caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca 1441 tctgtgcata tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg 1501 tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc 1561 gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga 1621 accattgaga tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag 1681 ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtagga 1741 atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag 1801 ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg 1861 gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat 1921 tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa 1981 catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt 2041 tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac 2101 atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc 2161 actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat 2221 ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt 2281 tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg 2341 gttataagtg aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag 2401 tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat 2461 cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg 2521 attttaatca ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct 2581 ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc 2641 tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt 2701 tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt 2761 gtataactca ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt 2821 tgctcctcta caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc 2881 gggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt 2941 ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact 3001 acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca 3061 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg 3121 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg 3181 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc 3241 ttactgtccc tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt 3301 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc 3361 gagctctcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga 3421 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc 3481 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca 3541 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg 3601 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga 3661 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat 3721 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt 3781 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg 3841 atactttctc ggcaggagca aggtgaaatc acaggagatc atgccccggc acttcgccca 3901 atagcagcca gtcccttccc gtttcagtga caacgtcgag cacagatgcg caaggaacgc 3961 ccgtcgtggc cagccacgat agccgcgctg cctcgtcccg cagttcattc agggcaccgg 4021 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg 4081 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag 4141 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg 4201 tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc 4261 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg 4321 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 4381 ttcgtcttca c //