LOCUS       pEB2-mRFP1*             3941 bp    DNA     circular UNA 27-DEC-2017
DEFINITION  pEB2 plasmid with mRFP1*.
ACCESSION   urn.local...be-87y7afo
VERSION     urn.local...be-87y7afo
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            .
FEATURES             Location/Qualifiers
     promoter        1..144
                     /created_by="User"
                     /modified_by="User"
                     /label="proC"
     motif           63..68
                     /created_by="User"
                     /modified_by="User"
                     /label="-35 Box"
     motif           86..91
                     /created_by="User"
                     /label="-10 Box"
     motif           98
                     /created_by="User"
                     /label="TransStart"
     misc_feature    145..170
                     /created_by="User"
                     /modified_by="User"
                     /label="UArbs"
     CDS             171..878
                     /created_by="User"
                     /modified_by="User"
                     /label="mRFP1*"
     motif           171..188
                     /created_by="User"
                     /modified_by="User"
                     /label="avGFP_N_terminus"
     motif           189..191
                     /created_by="User"
                     /label="N8"
     motif           195..197
                     /created_by="User"
                     /label="M->L (avoid internal transltion)"
     motif           243..245
                     /created_by="User"
                     /label="T21S"
     motif           303..305
                     /created_by="User"
                     /label="H41T"
     motif           306..308
                     /created_by="User"
                     /label="N42Q"
     motif           312..314
                     /created_by="User"
                     /label="V44A"
     motif           393..395
                     /created_by="User"
                     /label="V71A"
     motif           429..431
                     /created_by="User"
                     /modified_by="User"
                     /label="K83L"
     motif           531..533
                     /created_by="User"
                     /label="C117E"
     motif           552..554
                     /created_by="User"
                     /modified_by="User"
                     /label="F124L"
     motif           555..557
                     /created_by="User"
                     /modified_by="User"
                     /label="I125R"
     motif           561..563
                     /created_by="User"
                     /modified_by="User"
                     /label="V127T"
     motif           630..632
                     /created_by="User"
                     /label="L150M"
     motif           639..641
                     /created_by="User"
                     /label="R153E"
     motif           648..650
                     /created_by="User"
                     /label="V156A"
     motif           666..668
                     /created_by="User"
                     /label="H162K"
     motif           669..671
                     /created_by="User"
                     /label="K163M"
     motif           672..674
                     /created_by="User"
                     /label="A164R"
     motif           702..704
                     /created_by="User"
                     /label="L174D"
     motif           705..707
                     /created_by="User"
                     /label="V175A"
     motif           711..713
                     /created_by="User"
                     /modified_by="User"
                     /label="F177V"
     motif           717..719
                     /created_by="User"
                     /label="S179T"
     motif           720..722
                     /created_by="User"
                     /label="I180T"
     motif           756..758
                     /created_by="User"
                     /label="Y192A"
     motif           762..764
                     /created_by="User"
                     /label="Y194K"
     motif           765..767
                     /created_by="User"
                     /label="V195T"
     motif           771..773
                     /created_by="User"
                     /label="S197I"
     motif           831..833
                     /created_by="User"
                     /modified_by="User"
                     /label="T217A"
     motif           846..848
                     /created_by="User"
                     /modified_by="User"
                     /label="H222S"
     motif           849..851
                     /created_by="User"
                     /modified_by="User"
                     /label="L223T"
     motif           852..854
                     /created_by="User"
                     /modified_by="User"
                     /label="F224G"
     motif           855..875
                     /created_by="User"
                     /label="avGFP -C terminus"
     terminator      910..968
                     /created_by="User"
                     /modified_by="User"
                     /label="rrnB T1 terminator"
     rep_origin      complement(969..2852)
                     /created_by="User"
                     /modified_by="User"
                     /label="Origin of Rep. from pSC101"
     repeat_region   complement(992..1008)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     primer_bind     complement(1030..1053)
                     /Sequence="AAAGGGAAAACTGTCCATATGCAC"
                     /Hairpin_Tm="39.7"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="41.7"
                     /created_by="primer3"
                     /label="T1_from_mSC101_R02"
     repeat_region   complement(1110..1124)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     CDS             complement(1135..2085)
                     /note="unnamed protein product; rep101 protein"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA25276.1"
                     /db_xref="GI:42705"
                     /db_xref="GOA:Q47589"
                     /db_xref="InterPro:IPR000525"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="UniProtKB/TrEMBL:Q47589"
                     /modified_by="User"
                     /label="RepA"
     regulatory      complement(2087..2092)
                     /regulatory_class="ribosome_binding_site"
                     /note="Shine-Dalgarno sequence"
                     /label="Shine-Dalgarno sequence regulatory"
     repeat_region   complement(2087..2109)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2112..2133)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     regulatory      complement(2116..2121)
                     /regulatory_class="promoter"
                     /note="-10 region"
                     /label="-10 region regulatory"
     regulatory      complement(2138..2143)
                     /regulatory_class="promoter"
                     /note="-35 region"
                     /label="-35 region regulatory"
     repeat_region   complement(2733..2750)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2756..2779)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2790..2806)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2819..2832)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     terminator      complement(2858..2923)
                     /created_by="User"
                     /modified_by="User"
                     /label="t(o) terminator from pSS9"
     CDS             complement(2936..3730)
                     /gene="aph"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase (APH)
                     kanamycin/neomycin resistance protein"
                     /protein_id="AJW29743.1"
                     /db_xref="GI:768968340"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /modified_by="User"
                     /label="Aph (KmR)"
     modified_base   3200..3202
                     /created_by="User"
                     /label="R177H"
     modified_base   3446..3448
                     /created_by="User"
                     /modified_by="User"
                     /label="G95D"
     modified_base   3596..3598
                     /created_by="User"
                     /modified_by="User"
                     /label="P45T"
     promoter        complement(3819..3867)
                     /created_by="User"
                     /modified_by="User"
                     /label="aph (KmR) promoter"
     primer_bind     3823..3843
                     /Sequence="AGGGCTTCCCAACCTTACCAG"
                     /Hairpin_Tm="48.5"
                     /Tm="61.7"
                     /Self_Dimer_Tm="4.5"
                     /%GC="57.1"
                     /created_by="primer3"
                     /label="pZSeq2_Kan"
     primer_bind     3914..3933
                     /created_by="User"
                     /Sequence="GCGTATCACGAGGCCCTTTC"
                     /Hairpin_Tm="46.5"
                     /Tm="61.2"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="pZ_seq_frw"
ORIGIN      
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa cttttctaga tttaagaagg agatatacat atgagtaaag
      181 gagaagaaaa caacttagct gtgattaaag agttcatgcg cttcaaagtt cgcatggagg
      241 gttctgttaa cggtcacgag ttcgagatcg aaggcgaagg cgagggccgt ccgtatgaag
      301 gcacccagac cgccaaactg aaagtgacta aaggcggccc gctgcctttt gcgtgggaca
      361 tcctgagccc gcaatttcag tacggttcta aagcgtatgt taaacaccca gcggatatcc
      421 cggactatct gaagctgtct tttccggaag gtttcaagtg ggaacgcgta atgaattttg
      481 aagatggtgg tgtcgtgacc gtcactcagg actcctccct gcaggatggc gagttcatct
      541 ataaagttaa actgcgtggt actaattttc catctgatgg cccggtgatg cagaaaaaga
      601 cgatgggttg ggaggcgtct accgaacgca tgtatccgga agatggtgcg ctgaaaggcg
      661 aaattaaaat gcgcctgaaa ctgaaagatg gcggccatta tgacgctgaa gtgaaaacca
      721 cgtacatggc caagaaacct gtgcagctgc ctggcgcgta caagaccgat attaaactgg
      781 acatcacctc tcataatgaa gattatacga tcgtagagca atatgagcgc gcggagggtc
      841 gtcattctac cggtggcatg gatgaactat acaaataaat gtccagacct gcaggcatgc
      901 aagctctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      961 atctgttgaa atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg
     1021 ttttcatctg tgcatatgga cagttttccc tttgatatct aacggtgaac agttgttcta
     1081 cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat
     1141 ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga
     1201 gaacgaacca ttgagatcat gcttactttg catgtcactc aaaaattttg cctcaaaact
     1261 ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttacgtag
     1321 gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt
     1381 ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc
     1441 agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt
     1501 acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag
     1561 cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt
     1621 ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga
     1681 cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat
     1741 ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg
     1801 gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc
     1861 tctggttgct ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc
     1921 gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg
     1981 gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg
     2041 actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc
     2101 taggtgattt taatcactat accaattgag atgggctagt caatgataat tactagtcct
     2161 tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa
     2221 ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca
     2281 agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag
     2341 ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac
     2401 gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc
     2461 aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct
     2521 gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat
     2581 ggcactacag gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc
     2641 ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt
     2701 ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt
     2761 gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta
     2821 cccgtcttac tgtcaaccag acccgccagg attctcatgc ataaaaaacg cccggcggca
     2881 accgagcgtt ctgaattaat taatcatcgc gactgcagag atcccagagt ccttattaga
     2941 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     3001 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     3061 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     3121 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     3181 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     3241 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     3301 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     3361 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     3421 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     3481 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     3541 cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     3601 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     3661 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     3721 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc
     3781 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     3841 cagagggcgc cccagctggc aattccgacg tctaagaaac cattattatc atgacattaa
     3901 cctataaaaa taggcgtatc acgaggccct ttcgtcttca c
//
