LOCUS pEB2-mRFP1 3929 bp DNA circular UNA 27-DEC-2017 DEFINITION pEB2 plasmid with mRFP1. ACCESSION urn.local...b9-87y7a9z VERSION urn.local...b9-87y7a9z KEYWORDS . SOURCE Escherichia coli ORGANISM Escherichia coli . FEATURES Location/Qualifiers promoter 1..144 /created_by="User" /modified_by="User" /label="proC" motif 63..68 /created_by="User" /modified_by="User" /label="-35 Box" motif 86..91 /created_by="User" /label="-10 Box" motif 98 /created_by="User" /label="TransStart" misc_feature 145..170 /created_by="User" /modified_by="User" /label="UArbs" CDS 171..866 /created_by="User" /modified_by="User" /label="mRFP1" motif 174..176 /created_by="User" /label="R2A" motif 183..185 /created_by="User" /label="K5E" motif 186..188 /created_by="User" /label="N6D" motif 231..233 /created_by="User" /label="T21S" motif 291..293 /created_by="User" /label="H41T" motif 294..296 /created_by="User" /label="N42Q" motif 300..302 /created_by="User" /label="V44A" motif 381..383 /created_by="User" /label="V71A" motif 417..419 /created_by="User" /modified_by="User" /label="K83L" motif 519..521 /created_by="User" /label="C117E" motif 540..542 /created_by="User" /modified_by="User" /label="F124L" motif 543..545 /created_by="User" /modified_by="User" /label="I125R" motif 549..551 /created_by="User" /modified_by="User" /label="V127T" motif 618..620 /created_by="User" /label="L150M" motif 627..629 /created_by="User" /label="R153E" motif 636..638 /created_by="User" /label="V156A" motif 654..656 /created_by="User" /label="H162K" motif 657..659 /created_by="User" /label="K163M" motif 660..662 /created_by="User" /label="A164R" motif 690..692 /created_by="User" /label="L174D" motif 693..695 /created_by="User" /label="V175A" motif 699..701 /created_by="User" /modified_by="User" /label="F177V" motif 705..707 /created_by="User" /label="S179T" motif 708..710 /created_by="User" /label="I180T" motif 744..746 /created_by="User" /label="Y192A" motif 750..752 /created_by="User" /label="Y194K" motif 753..755 /created_by="User" /label="V195T" motif 759..761 /created_by="User" /label="S197I" motif 819..821 /created_by="User" /modified_by="User" /label="T217A" motif 834..836 /created_by="User" /modified_by="User" /label="H222S" motif 837..839 /created_by="User" /modified_by="User" /label="L223T" motif 840..842 /created_by="User" /modified_by="User" /label="F224G" motif 843..863 /created_by="User" /label="avGFP -C terminus" terminator 898..956 /created_by="User" /modified_by="User" /label="rrnB T1 terminator" rep_origin complement(957..2840) /created_by="User" /modified_by="User" /label="Origin of Rep. from pSC101" repeat_region complement(980..996) /note="inverted repeat" /label="inverted repeat repeat region" primer_bind complement(1018..1041) /Sequence="AAAGGGAAAACTGTCCATATGCAC" /Hairpin_Tm="39.7" /Tm="59.8" /Self_Dimer_Tm="None" /%GC="41.7" /created_by="primer3" /label="T1_from_mSC101_R02" repeat_region complement(1098..1112) /note="inverted repeat" /label="inverted repeat repeat region" CDS complement(1123..2073) /note="unnamed protein product; rep101 protein" /codon_start=1 /transl_table=11 /protein_id="CAA25276.1" /db_xref="GI:42705" /db_xref="GOA:Q47589" /db_xref="InterPro:IPR000525" /db_xref="InterPro:IPR011991" /db_xref="UniProtKB/TrEMBL:Q47589" /modified_by="User" /label="RepA" regulatory complement(2075..2080) /regulatory_class="ribosome_binding_site" /note="Shine-Dalgarno sequence" /label="Shine-Dalgarno sequence regulatory" repeat_region complement(2075..2097) /note="inverted repeat" /label="inverted repeat repeat region" repeat_region complement(2100..2121) /note="inverted repeat" /label="inverted repeat repeat region" regulatory complement(2104..2109) /regulatory_class="promoter" /note="-10 region" /label="-10 region regulatory" regulatory complement(2126..2131) /regulatory_class="promoter" /note="-35 region" /label="-35 region regulatory" repeat_region complement(2721..2738) /note="inverted repeat" /label="inverted repeat repeat region" repeat_region complement(2744..2767) /note="inverted repeat" /label="inverted repeat repeat region" repeat_region complement(2778..2794) /note="inverted repeat" /label="inverted repeat repeat region" repeat_region complement(2807..2820) /note="inverted repeat" /label="inverted repeat repeat region" terminator complement(2846..2911) /created_by="User" /modified_by="User" /label="t(o) terminator from pSS9" CDS complement(2924..3718) /gene="aph" /codon_start=1 /transl_table=11 /product="Aminoglycoside 3'-phosphotransferase (APH) kanamycin/neomycin resistance protein" /protein_id="AJW29743.1" /db_xref="GI:768968340" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /modified_by="User" /label="Aph (KmR)" modified_base 3188..3190 /created_by="User" /label="R177H" modified_base 3434..3436 /created_by="User" /modified_by="User" /label="G95D" modified_base 3584..3586 /created_by="User" /modified_by="User" /label="P45T" promoter complement(3807..3855) /created_by="User" /modified_by="User" /label="aph (KmR) promoter" primer_bind 3811..3831 /Sequence="AGGGCTTCCCAACCTTACCAG" /Hairpin_Tm="48.5" /Tm="61.7" /Self_Dimer_Tm="4.5" /%GC="57.1" /created_by="primer3" /label="pZSeq2_Kan" primer_bind 3902..3921 /created_by="User" /Sequence="GCGTATCACGAGGCCCTTTC" /Hairpin_Tm="46.5" /Tm="61.2" /Self_Dimer_Tm="None" /%GC="60.0" /created_by="primer3" /modified_by="User" /label="pZ_seq_frw" ORIGIN 1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata 61 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct 121 ctacaaataa ttttgtttaa cttttctaga tttaagaagg agatatacat atggccagca 181 gcgaggacgt gattaaagag ttcatgcgct tcaaagttcg catggagggt tctgttaacg 241 gtcacgagtt cgagatcgaa ggcgaaggcg agggccgtcc gtatgaaggc acccagaccg 301 ccaaactgaa agtgactaaa ggcggcccgc tgccttttgc gtgggacatc ctgagcccgc 361 aatttcagta cggttctaaa gcgtatgtta aacacccagc ggatatcccg gactatctga 421 agctgtcttt tccggaaggt ttcaagtggg aacgcgtaat gaattttgaa gatggtggtg 481 tcgtgaccgt cactcaggac tcctccctgc aggatggcga gttcatctat aaagttaaac 541 tgcgtggtac taattttcca tctgatggcc cggtgatgca gaaaaagacg atgggttggg 601 aggcgtctac cgaacgcatg tatccggaag atggtgcgct gaaaggcgaa attaaaatgc 661 gcctgaaact gaaagatggc ggccattatg acgctgaagt gaaaaccacg tacatggcca 721 agaaacctgt gcagctgcct ggcgcgtaca agaccgatat taaactggac atcacctctc 781 ataatgaaga ttatacgatc gtagagcaat atgagcgcgc ggagggtcgt cattctaccg 841 gtggcatgga tgaactatac aaataaatgt ccagacctgc aggcatgcaa gctctagagg 901 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgaaat 961 gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg 1021 catatggaca gttttccctt tgatatctaa cggtgaacag ttgttctact tttgtttgtt 1081 agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt 1141 agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt 1201 gagatcatgc ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat 1261 ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga 1321 tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt 1381 tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc 1441 tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt 1501 caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa 1561 cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc 1621 ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc 1681 cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa 1741 aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa 1801 gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt 1861 agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt attggttata 1921 agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc 1981 cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag 2041 ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta 2101 atcactatac caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt 2161 tgtgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc 2221 ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat 2281 ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa 2341 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc 2401 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa 2461 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg 2521 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc 2581 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct 2641 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc 2701 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc 2761 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg 2821 tcaaccagac ccgccaggat tctcatgcat aaaaaacgcc cggcggcaac cgagcgttct 2881 gaattaatta atcatcgcga ctgcagagat cccagagtcc ttattagaag aactcgtcaa 2941 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga 3001 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt 3061 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat 3121 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt 3181 cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt 3241 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc 3301 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca 3361 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct 3421 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca 3481 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca 3541 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg 3601 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga 3661 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc 3721 gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg 3781 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc 3841 cagctggcaa ttccgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 3901 ggcgtatcac gaggcccttt cgtcttcac //