LOCUS       pEB2-mRuby3             3944 bp    DNA     circular UNA 27-DEC-2017
DEFINITION  pEB2 plasmid with mRuby3.
ACCESSION   urn.local...bj-87y7akd
VERSION     urn.local...bj-87y7akd
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            .
FEATURES             Location/Qualifiers
     promoter        1..144
                     /created_by="User"
                     /modified_by="User"
                     /label="proC"
     motif           63..68
                     /created_by="User"
                     /modified_by="User"
                     /label="-35 Box"
     motif           86..91
                     /created_by="User"
                     /label="-10 Box"
     motif           98
                     /created_by="User"
                     /label="TransStart"
     misc_feature    145..170
                     /created_by="User"
                     /modified_by="User"
                     /label="UArbs"
     CDS             171..881
                     /created_by="User"
                     /modified_by="User"
                     /label="mRuby3"
     motif           171..188
                     /created_by="User"
                     /modified_by="User"
                     /label="avGFP_N_terminus"
     motif           189..212
                     /created_by="User"
                     /label="common to all mRuby derivatives"
     motif           213..215
                     /created_by="User"
                     /modified_by="User"
                     /label="M12K"
     motif           243..245
                     /created_by="User"
                     /modified_by="User"
                     /label="Y22H"
     motif           270..272
                     /created_by="User"
                     /modified_by="User"
                     /label="D31E"
     motif           276..278
                     /created_by="User"
                     /label="N33R"
     motif           285..287
                     /created_by="User"
                     /label="M36E"
     motif           291..293
                     /created_by="User"
                     /label="T38V"
     motif           297..299
                     /created_by="User"
                     /label="T40V"
     motif           315..317
                     /created_by="User"
                     /modified_by="User"
                     /label="V46I"
     motif           378..380
                     /created_by="User"
                     /modified_by="User"
                     /label="K67R"
     motif           393..395
                     /created_by="User"
                     /label="H72Y"
     motif           396..398
                     /created_by="User"
                     /label="T73P"
     motif           399..401
                     /created_by="User"
                     /label="K74A"
     motif           402..404
                     /created_by="User"
                     /label="G75D"
     motif           483..485
                     /created_by="User"
                     /label="F102V"
     motif           492..494
                     /created_by="User"
                     /label="M105T"
     motif           519..521
                     /created_by="User"
                     /modified_by="User"
                     /label="C114E"
     motif           531..533
                     /created_by="User"
                     /label="H118N"
     motif           534..536
                     /created_by="User"
                     /label="A119V"
     motif           543..545
                     /created_by="User"
                     /label="T122R"
     motif           570..572
                     /created_by="User"
                     /label="A131P"
     motif           618..620
                     /created_by="User"
                     /label="L147M"
     motif           651..653
                     /created_by="User"
                     /label="S158T"
     motif           654..656
                     /created_by="User"
                     /label="Q159D"
     motif           657..659
                     /created_by="User"
                     /label="M160I"
     motif           666..668
                     /created_by="User"
                     /label="N163K"
     motif           684..686
                     /created_by="User"
                     /label="Y169H"
     motif           690..692
                     /created_by="User"
                     /label="S171H"
     motif           696..698
                     /created_by="User"
                     /label="S173N"
     motif           702..704
                     /created_by="User"
                     /label="E175V"
     motif           732..734
                     /created_by="User"
                     /modified_by="User"
                     /label="E185G"
     motif           738..740
                     /created_by="User"
                     /label="F187I"
     motif           753..755
                     /created_by="User"
                     /label="F192V"
     motif           759..761
                     /created_by="User"
                     /label="F194A"
     motif           783..785
                     /created_by="User"
                     /label="L202I"
     motif           798..800
                     /created_by="User"
                     /label="K207N"
     motif           804..806
                     /created_by="User"
                     /modified_by="User"
                     /label="M209T"
     motif           807..809
                     /created_by="User"
                     /label="F210Y"
     motif           819..821
                     /created_by="User"
                     /label="H214R"
     motif           825..827
                     /created_by="User"
                     /label="H216V"
     motif           840..842
                     /created_by="User"
                     /label="F221Y"
     motif           843..845
                     /created_by="User"
                     /label="C222S"
     motif           846..848
                     /created_by="User"
                     /label="D223N"
     motif           849..857
                     /created_by="User"
                     /label="End of mRuby1"
     motif           858..878
                     /created_by="User"
                     /label="avGFP -C terminus"
     terminator      913..971
                     /created_by="User"
                     /modified_by="User"
                     /label="rrnB T1 terminator"
     rep_origin      complement(972..2855)
                     /created_by="User"
                     /modified_by="User"
                     /label="Origin of Rep. from pSC101"
     repeat_region   complement(995..1011)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     primer_bind     complement(1033..1056)
                     /Sequence="AAAGGGAAAACTGTCCATATGCAC"
                     /Hairpin_Tm="39.7"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="41.7"
                     /created_by="primer3"
                     /label="T1_from_mSC101_R02"
     repeat_region   complement(1113..1127)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     CDS             complement(1138..2088)
                     /note="unnamed protein product; rep101 protein"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA25276.1"
                     /db_xref="GI:42705"
                     /db_xref="GOA:Q47589"
                     /db_xref="InterPro:IPR000525"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="UniProtKB/TrEMBL:Q47589"
                     /modified_by="User"
                     /label="RepA"
     regulatory      complement(2090..2095)
                     /regulatory_class="ribosome_binding_site"
                     /note="Shine-Dalgarno sequence"
                     /label="Shine-Dalgarno sequence regulatory"
     repeat_region   complement(2090..2112)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2115..2136)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     regulatory      complement(2119..2124)
                     /regulatory_class="promoter"
                     /note="-10 region"
                     /label="-10 region regulatory"
     regulatory      complement(2141..2146)
                     /regulatory_class="promoter"
                     /note="-35 region"
                     /label="-35 region regulatory"
     repeat_region   complement(2736..2753)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2759..2782)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2793..2809)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2822..2835)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     terminator      complement(2861..2926)
                     /created_by="User"
                     /modified_by="User"
                     /label="t(o) terminator from pSS9"
     CDS             complement(2939..3733)
                     /gene="aph"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase (APH)
                     kanamycin/neomycin resistance protein"
                     /protein_id="AJW29743.1"
                     /db_xref="GI:768968340"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /modified_by="User"
                     /label="Aph (KmR)"
     modified_base   3203..3205
                     /created_by="User"
                     /label="R177H"
     modified_base   3449..3451
                     /created_by="User"
                     /modified_by="User"
                     /label="G95D"
     modified_base   3599..3601
                     /created_by="User"
                     /modified_by="User"
                     /label="P45T"
     promoter        complement(3822..3870)
                     /created_by="User"
                     /modified_by="User"
                     /label="aph (KmR) promoter"
     primer_bind     3826..3846
                     /Sequence="AGGGCTTCCCAACCTTACCAG"
                     /Hairpin_Tm="48.5"
                     /Tm="61.7"
                     /Self_Dimer_Tm="4.5"
                     /%GC="57.1"
                     /created_by="primer3"
                     /label="pZSeq2_Kan"
     primer_bind     3917..3936
                     /created_by="User"
                     /Sequence="GCGTATCACGAGGCCCTTTC"
                     /Hairpin_Tm="46.5"
                     /Tm="61.2"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="pZ_seq_frw"
ORIGIN      
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa cttttctaga tttaagaagg agatatacat atgagtaaag
      181 gagaagaact gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg
      241 gccaccaatt caaatgcaca ggtgaaggag aaggcagacc gtacgaggga gtgcaagtga
      301 tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt
      361 cgttcatgta tggcagccgt acctttatca agtacccggc cgacatccct gatttcttta
      421 aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag
      481 tcgtcaccgt cacgcaggac accagccttg aggatggcga gctcgtctac aacgtcaagg
      541 tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaagacc aagggttggg
      601 agcctaatac agagatgatg tatccagcag atggtggtct gagaggatac actgacatcg
      661 cactgaaagt tgatggtggt ggccatctgc actgcaactt cgtgacaact tacaggtcaa
      721 aaaagaccgt cgggaacatc aagatgcccg gtgtccatgc cgttgatcac cgcctggaaa
      781 ggatcgagga gagtgacaat gaaacctacg tagtgcaaag agaagtggca gttgccaaat
      841 acagcaacct tggtggtggc atggatgaac tatacaaata aatgtccaga cctgcaggca
      901 tgcaagctct agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      961 tttatctgtt gaaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca
     1021 ccgttttcat ctgtgcatat ggacagtttt ccctttgata tctaacggtg aacagttgtt
     1081 ctacttttgt ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca
     1141 gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca
     1201 tgagaacgaa ccattgagat catgcttact ttgcatgtca ctcaaaaatt ttgcctcaaa
     1261 actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttacg
     1321 taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt
     1381 gttctcaagt tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt
     1441 atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc
     1501 tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc
     1561 aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt
     1621 cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa
     1681 agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa
     1741 tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca
     1801 ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag
     1861 ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg
     1921 agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt
     1981 ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata
     2041 gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg
     2101 gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt
     2161 ccttttcctt tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt
     2221 aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat
     2281 tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc
     2341 cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca
     2401 aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct
     2461 cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc
     2521 gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta
     2581 aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa
     2641 agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac
     2701 tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc
     2761 cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc
     2821 ttacccgtct tactgtcaac cagacccgcc aggattctca tgcataaaaa acgcccggcg
     2881 gcaaccgagc gttctgaatt aattaatcat cgcgactgca gagatcccag agtccttatt
     2941 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     3001 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     3061 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     3121 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     3181 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
     3241 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     3301 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     3361 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     3421 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     3481 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     3541 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     3601 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     3661 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     3721 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
     3781 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     3841 taccagaggg cgccccagct ggcaattccg acgtctaaga aaccattatt atcatgacat
     3901 taacctataa aaataggcgt atcacgaggc cctttcgtct tcac
//
