LOCUS pEB2-2ndVal_mKate2 3932 bp DNA circular UNA 27-DEC-2017 DEFINITION pEB2 plasmid with 2ndVal mKate2. ACCESSION urn.local...ey-87ycevf VERSION urn.local...ey-87ycevf KEYWORDS . SOURCE Escherichia coli ORGANISM Escherichia coli . FEATURES Location/Qualifiers promoter 1..144 /created_by="User" /modified_by="User" /label="proC" motif 63..68 /created_by="User" /modified_by="User" /label="-35 Box" motif 86..91 /created_by="User" /label="-10 Box" motif 98 /created_by="User" /label="TransStart" misc_feature 145..170 /created_by="User" /modified_by="User" /label="UArbs" CDS 171..869 /note="derived from TagRFP, Genbank accession EF606900" /codon_start=1 /transl_table=11 /product="monomeric red fluorescent protein TagRFP-T" /protein_id="ACD03281.1" /db_xref="GI:187373121" /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQT MRIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYED GGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEG RTDMALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQH EVAVARYCDLPSKLGHKLNGMDELYK" /modified_by="User" /label="2ndVal mKate2" motif 174..176 /created_by="User" /modified_by="User" /label="2nd Val" motif 267..269 /created_by="User" /modified_by="User" /label="R32G" motif 297..299 /created_by="User" /modified_by="User" /label="K42R" motif 306..308 /created_by="User" /modified_by="User" /label="V45A" motif 408..410 /created_by="User" /modified_by="User" /label="L79F" motif 450..452 /created_by="User" /modified_by="User" /label="I93V" motif 507..509 /created_by="User" /modified_by="User" /label="N112D" motif 516..518 /created_by="User" /modified_by="User" /label="I115L" motif 537..539 /created_by="User" /modified_by="User" /label="N122R" motif 564..566 /created_by="User" /modified_by="User" /label="S131P" motif 600..602 /created_by="User" /modified_by="User" /label="N143S" motif 609..611 /created_by="User" /modified_by="User" /label="M146T" motif 636..638 /created_by="User" /modified_by="User" /label="R155E" motif 642..644 /created_by="User" /modified_by="User" /label="H157R" motif 645..647 /created_by="User" /modified_by="User" /label="S158A" motif 648..650 /created_by="User" /modified_by="User" /label="Q159D" motif 678..680 /created_by="User" /modified_by="User" /label="Y169H" motif 684..686 /created_by="User" /modified_by="User" /label="H171I" motif 690..692 /created_by="User" /modified_by="User" /label="S173N" motif 693..695 /created_by="User" /modified_by="User" /label="F174L" motif 747..749 /created_by="User" /modified_by="User" /label="F192V" motif 750..752 /created_by="User" /modified_by="User" /label="H193Y" motif 753..755 /created_by="User" /modified_by="User" /label="F194Y" motif 762..764 /created_by="User" /modified_by="User" /label="H197R" motif 819..821 /created_by="User" /modified_by="User" /label="M216V" motif 831..833 /created_by="User" /modified_by="User" /label="K220R" terminator 901..959 /created_by="User" /modified_by="User" /label="rrnB T1 terminator" rep_origin complement(960..2843) /created_by="User" /modified_by="User" /label="Origin of Rep. from pSC101" repeat_region complement(983..999) /note="inverted repeat" /label="inverted repeat repeat region" primer_bind complement(1021..1044) /Sequence="AAAGGGAAAACTGTCCATATGCAC" /Hairpin_Tm="39.7" /Tm="59.8" /Self_Dimer_Tm="None" /%GC="41.7" /created_by="primer3" /label="T1_from_mSC101_R02" repeat_region complement(1101..1115) /note="inverted repeat" /label="inverted repeat repeat region" CDS complement(1126..2076) /note="unnamed protein product; rep101 protein" /codon_start=1 /transl_table=11 /protein_id="CAA25276.1" /db_xref="GI:42705" /db_xref="GOA:Q47589" /db_xref="InterPro:IPR000525" /db_xref="InterPro:IPR011991" /db_xref="UniProtKB/TrEMBL:Q47589" /modified_by="User" /label="RepA" regulatory complement(2078..2083) /regulatory_class="ribosome_binding_site" /note="Shine-Dalgarno sequence" /label="Shine-Dalgarno sequence regulatory" repeat_region complement(2078..2100) /note="inverted repeat" /label="inverted repeat repeat region" repeat_region complement(2103..2124) /note="inverted repeat" /label="inverted repeat repeat region" regulatory complement(2107..2112) /regulatory_class="promoter" /note="-10 region" /label="-10 region regulatory" regulatory complement(2129..2134) /regulatory_class="promoter" /note="-35 region" /label="-35 region regulatory" repeat_region complement(2724..2741) /note="inverted repeat" /label="inverted repeat repeat region" repeat_region complement(2747..2770) /note="inverted repeat" /label="inverted repeat repeat region" repeat_region complement(2781..2797) /note="inverted repeat" /label="inverted repeat repeat region" repeat_region complement(2810..2823) /note="inverted repeat" /label="inverted repeat repeat region" terminator complement(2849..2914) /created_by="User" /modified_by="User" /label="t(o) terminator from pSS9" CDS complement(2927..3721) /gene="aph" /codon_start=1 /transl_table=11 /product="Aminoglycoside 3'-phosphotransferase (APH) kanamycin/neomycin resistance protein" /protein_id="AJW29743.1" /db_xref="GI:768968340" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /modified_by="User" /label="Aph (KmR)" modified_base 3191..3193 /created_by="User" /label="R177H" modified_base 3437..3439 /created_by="User" /modified_by="User" /label="G95D" modified_base 3587..3589 /created_by="User" /modified_by="User" /label="P45T" promoter complement(3810..3858) /created_by="User" /modified_by="User" /label="aph (KmR) promoter" primer_bind 3814..3834 /Sequence="AGGGCTTCCCAACCTTACCAG" /Hairpin_Tm="48.5" /Tm="61.7" /Self_Dimer_Tm="4.5" /%GC="57.1" /created_by="primer3" /label="pZSeq2_Kan" primer_bind 3905..3924 /created_by="User" /Sequence="GCGTATCACGAGGCCCTTTC" /Hairpin_Tm="46.5" /Tm="61.2" /Self_Dimer_Tm="None" /%GC="60.0" /created_by="primer3" /modified_by="User" /label="pZ_seq_frw" ORIGIN 1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata 61 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct 121 ctacaaataa ttttgtttaa cttttctaga tttaagaagg agatatacat atggtgtctg 181 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc 241 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa 301 tcaaggcggt cgagggcggc cctttaccct tcgccttcga catcctggct accagcttca 361 tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt 421 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga 481 ccgctaccca ggacaccagc ttacaggacg gctgcttgat ctacaacgtc aagatcagag 541 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacattaggc tgggaggcct 601 ccaccgagac cctgtacccc gctgacggcg gcctggaagg cagagccgac atggccctga 661 agttagtggg cgggggccac ctgatctgca acttgaagac cacatacaga tccaagaaac 721 ccgctaagaa cttgaagatg cccggcgtct actatgtgga cagaagactg gaaagaatca 781 aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg 841 acctgcctag caaactgggg cacagataaa tgtccagacc tgcaggcatg caagctctag 901 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttga 961 aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct 1021 gtgcatatgg acagttttcc ctttgatatc taacggtgaa cagttgttct acttttgttt 1081 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta 1141 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc 1201 attgagatca tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg 1261 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc 1321 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc 1381 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg 1441 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg 1501 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat 1561 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag 1621 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg 1681 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact 1741 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc 1801 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc 1861 tttagctaat acaccataag cattttccct actgatgttc atcatctgag cgtattggtt 1921 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag 1981 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc 2041 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt 2101 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg 2161 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag 2221 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat 2281 aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta 2341 taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc 2401 tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg 2461 caaatcgctg aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc 2521 gtgacattca gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca 2581 ggcgcctttt atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg 2641 gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc 2701 agcagttcct gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca 2761 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta 2821 ctgtcaacca gacccgccag gattctcatg cataaaaaac gcccggcggc aaccgagcgt 2881 tctgaattaa ttaatcatcg cgactgcaga gatcccagag tccttattag aagaactcgt 2941 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga 3001 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta 3061 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc 3121 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc 3181 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct 3241 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga 3301 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc 3361 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat 3421 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga 3481 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct 3541 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg 3601 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc 3661 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca 3721 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc 3781 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg 3841 ccccagctgg caattccgac gtctaagaaa ccattattat catgacatta acctataaaa 3901 ataggcgtat cacgaggccc tttcgtcttc ac //