LOCUS       pEB2-2ndVal_Katushka9-5        3932 bp    DNA     circular UNA 27-DEC-2017
DEFINITION  pEB2 plasmid with 2ndVal Katushka9-5.
ACCESSION   urn.local...ex-87ycevd
VERSION     urn.local...ex-87ycevd
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            .
FEATURES             Location/Qualifiers
     promoter        1..144
                     /created_by="User"
                     /modified_by="User"
                     /label="proC"
     motif           63..68
                     /created_by="User"
                     /modified_by="User"
                     /label="-35 Box"
     motif           86..91
                     /created_by="User"
                     /label="-10 Box"
     motif           98
                     /created_by="User"
                     /label="TransStart"
     misc_feature    145..170
                     /created_by="User"
                     /modified_by="User"
                     /label="UArbs"
     CDS             171..869
                     /note="derived from TagRFP, Genbank accession EF606900"
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric red fluorescent protein TagRFP-T"
                     /protein_id="ACD03281.1"
                     /db_xref="GI:187373121"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQT
                     MRIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYED
                     GGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEG
                     RTDMALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQH
                     EVAVARYCDLPSKLGHKLNGMDELYK"
                     /modified_by="User"
                     /label="2ndVal Katushka9-5"
     motif           174..176
                     /created_by="User"
                     /modified_by="User"
                     /label="2nd Val"
     motif           189..191
                     /created_by="User"
                     /modified_by="User"
                     /label="K6S"
     motif           234..236
                     /created_by="User"
                     /modified_by="User"
                     /label="N21D"
     motif           267..269
                     /created_by="User"
                     /modified_by="User"
                     /label="R32G"
     motif           408..410
                     /created_by="User"
                     /modified_by="User"
                     /label="L79F"
     motif           516..518
                     /created_by="User"
                     /modified_by="User"
                     /label="I115L"
     motif           564..566
                     /created_by="User"
                     /modified_by="User"
                     /label="S131P"
     motif           600..602
                     /created_by="User"
                     /modified_by="User"
                     /label="N143S"
     motif           627..629
                     /created_by="User"
                     /modified_by="User"
                     /label="G152S"
     motif           693..695
                     /created_by="User"
                     /modified_by="User"
                     /label="F174L"
     motif           750..752
                     /created_by="User"
                     /modified_by="User"
                     /label="H193Y"
     motif           762..764
                     /created_by="User"
                     /modified_by="User"
                     /label="H197R"
     motif           765..767
                     /created_by="User"
                     /modified_by="User"
                     /label="R198K"
     motif           831..833
                     /created_by="User"
                     /modified_by="User"
                     /label="K220R"
     motif           864..866
                     /created_by="User"
                     /modified_by="User"
                     /label="R231S"
     terminator      901..959
                     /created_by="User"
                     /modified_by="User"
                     /label="rrnB T1 terminator"
     rep_origin      complement(960..2843)
                     /created_by="User"
                     /modified_by="User"
                     /label="Origin of Rep. from pSC101"
     repeat_region   complement(983..999)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     primer_bind     complement(1021..1044)
                     /Sequence="AAAGGGAAAACTGTCCATATGCAC"
                     /Hairpin_Tm="39.7"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="41.7"
                     /created_by="primer3"
                     /label="T1_from_mSC101_R02"
     repeat_region   complement(1101..1115)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     CDS             complement(1126..2076)
                     /note="unnamed protein product; rep101 protein"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA25276.1"
                     /db_xref="GI:42705"
                     /db_xref="GOA:Q47589"
                     /db_xref="InterPro:IPR000525"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="UniProtKB/TrEMBL:Q47589"
                     /modified_by="User"
                     /label="RepA"
     regulatory      complement(2078..2083)
                     /regulatory_class="ribosome_binding_site"
                     /note="Shine-Dalgarno sequence"
                     /label="Shine-Dalgarno sequence regulatory"
     repeat_region   complement(2078..2100)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2103..2124)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     regulatory      complement(2107..2112)
                     /regulatory_class="promoter"
                     /note="-10 region"
                     /label="-10 region regulatory"
     regulatory      complement(2129..2134)
                     /regulatory_class="promoter"
                     /note="-35 region"
                     /label="-35 region regulatory"
     repeat_region   complement(2724..2741)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2747..2770)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2781..2797)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     repeat_region   complement(2810..2823)
                     /note="inverted repeat"
                     /label="inverted repeat repeat region"
     terminator      complement(2849..2914)
                     /created_by="User"
                     /modified_by="User"
                     /label="t(o) terminator from pSS9"
     CDS             complement(2927..3721)
                     /gene="aph"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase (APH)
                     kanamycin/neomycin resistance protein"
                     /protein_id="AJW29743.1"
                     /db_xref="GI:768968340"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /modified_by="User"
                     /label="Aph (KmR)"
     modified_base   3191..3193
                     /created_by="User"
                     /label="R177H"
     modified_base   3437..3439
                     /created_by="User"
                     /modified_by="User"
                     /label="G95D"
     modified_base   3587..3589
                     /created_by="User"
                     /modified_by="User"
                     /label="P45T"
     promoter        complement(3810..3858)
                     /created_by="User"
                     /modified_by="User"
                     /label="aph (KmR) promoter"
     primer_bind     3814..3834
                     /Sequence="AGGGCTTCCCAACCTTACCAG"
                     /Hairpin_Tm="48.5"
                     /Tm="61.7"
                     /Self_Dimer_Tm="4.5"
                     /%GC="57.1"
                     /created_by="primer3"
                     /label="pZSeq2_Kan"
     primer_bind     3905..3924
                     /created_by="User"
                     /Sequence="GCGTATCACGAGGCCCTTTC"
                     /Hairpin_Tm="46.5"
                     /Tm="61.2"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="pZ_seq_frw"
ORIGIN      
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa cttttctaga tttaagaagg agatatacat atggtgtctg
      181 agctgattag cgagaacatg cacatgaagc tgtacatgga gggcaccgtg aacgaccacc
      241 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgaaga
      301 tcaaggtggt cgagggcggc cctttaccct tcgccttcga catcctggct accagcttca
      361 tgtacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt
      421 ccttccctga gggcttcaca tgggagagaa tcaccacata cgaagacggg ggcgtgctga
      481 ccgctaccca ggacaccagc ctgcagaacg gctgcctgat ctacaacgtc aagatcaacg
      541 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactgggc tgggaggcca
      601 gcaccgagat gctgtacccc gctgacagcg gcctgagggg ccacagccag atggccctga
      661 agttagtggg cgggggctac ctgcactgca gcttgaagac cacatacaga tccaagaaac
      721 ccgctaagaa cttgaagatg cccggcttct acttcgtgga caggaaactg gaaagaatca
      781 aggaggccga caaagagacc tacgtcgagc agcacgagat ggctgtggcc aggtactgcg
      841 acctgcctag caaactgggg cacagctaaa tgtccagacc tgcaggcatg caagctctag
      901 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttga
      961 aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct
     1021 gtgcatatgg acagttttcc ctttgatatc taacggtgaa cagttgttct acttttgttt
     1081 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta
     1141 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc
     1201 attgagatca tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg
     1261 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc
     1321 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc
     1381 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg
     1441 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg
     1501 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat
     1561 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag
     1621 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg
     1681 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact
     1741 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc
     1801 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc
     1861 tttagctaat acaccataag cattttccct actgatgttc atcatctgag cgtattggtt
     1921 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag
     1981 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc
     2041 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt
     2101 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg
     2161 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag
     2221 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat
     2281 aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta
     2341 taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc
     2401 tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg
     2461 caaatcgctg aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc
     2521 gtgacattca gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca
     2581 ggcgcctttt atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg
     2641 gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc
     2701 agcagttcct gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca
     2761 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta
     2821 ctgtcaacca gacccgccag gattctcatg cataaaaaac gcccggcggc aaccgagcgt
     2881 tctgaattaa ttaatcatcg cgactgcaga gatcccagag tccttattag aagaactcgt
     2941 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     3001 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     3061 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     3121 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     3181 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     3241 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     3301 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     3361 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     3421 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     3481 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct
     3541 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     3601 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     3661 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     3721 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3781 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3841 ccccagctgg caattccgac gtctaagaaa ccattattat catgacatta acctataaaa
     3901 ataggcgtat cacgaggccc tttcgtcttc ac
//
