LOCUS       LZF1718_pAAV_hSy        6101 bp ds-DNA     circular     01-MAY-2015
DEFINITION  .
ACCESSION   urn.local...dn-7ceo078
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     promoter        151..635
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="hSyn promoter"
     misc_feature    154..159
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    622..640
                     /Color="#ff0000"
                     /created_by="User"
                     /label="LZF54_F"
     source          <636..>2493
                     /organism="pAAV-Syn-Chronos-tdTomato"
                     /mol_type="other DNA"
     misc_feature    636..641
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    642..675
                     /created_by="User"
                     /label="FAS SITE"
     misc_feature    676..683
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     misc_feature    687..689
                     /Color="#df2bff"
                     /created_by="User"
                     /label="ATG"
     misc_feature    690..1634
                     /Color="#00ff00"
                     /created_by="User"
                     /label="Cheriff"
     misc_feature    1555..1574
                     /created_by="User"
                     /label="sp6"
     misc_feature    1702..1718
                     /created_by="User"
                     /label="R_CFP"
     misc_feature    <1704..2417
                     /created_by="User"
                     /modified_by="User"
                     /label="CFP"
     misc_feature    complement(2418..2420)
                     /created_by="User"
                     /label="reverted stop condon"
     misc_feature    complement(2421..2423)
                     /created_by="User"
                     /label="stop codon"
     misc_feature    2424..2429
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     misc_feature    2433..2466
                     /created_by="User"
                     /modified_by="User"
                     /label="FAS SITE"
     misc_feature    2468..2473
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2469..2493)
                     /created_by="User"
                     /Color="#ff0000"
                     /modified_by="User"
                     /label="LZF54_R_61C"
     misc_feature    2480..2485
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    2494..3081
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE"
     misc_feature    3098..3103
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     polyA_signal    3104..3335
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="bGH-Short polyA"
     misc_feature    3107..3112
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, SstI"
                     /note="Geneious type: restriction site"
     misc_feature    3324..3329
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI"
                     /note="Geneious type: restriction site"
     misc_feature    3364..3504
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      3596..3902
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="F1 Origin"
     misc_feature    4421..6101
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     misc_feature    4725..4730
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    4836..4841
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cacaacttcg tatatacctt
      661 tctatacgaa gttgtggcgc gccaccatgg gcggagctcc tgctccagac gctcacagcg
      721 ccccacctgg aaacgattct gccggaggca gtgagtacca tgccccagct ggatatcaag
      781 tgaatccacc ctaccacccc gtgcatgggt atgaggaaca gtgcagctcc atctacatct
      841 actatggggc cctgtgggag caggaaacag ctaggggctt ccagtggttt gccgtgttcc
      901 tgtctgccct gtttctggct ttctacggct ggcacgccta taaggccagc gtgggatggg
      961 aggaagtgta cgtgtgctcc gtggagctga tcaaagtgat tctggagatc tatttcgagt
     1021 tcaccagtcc tgctatgctg ttcctgtacg gagggaacat taccccatgg ctgagatatg
     1081 ccgaatggct gctgacatgt cccgtgatcc tgattcatct gtctaacatc accggcctga
     1141 gtgaggcata caataagcgg acaatggctc tgctggtgtc cgacctggga actatttgca
     1201 tgggagtgac agccgctctg gccactgggt gggtgaagtg gctgttttac tgtatcggcc
     1261 tggtgtatgg aacccagaca ttctacaacg ctggaatcat ctacgtggag tcttactata
     1321 tcatgcctgc cggcggctgt aagaaactgg tgctggccat gactgccgtg tactattcta
     1381 gttggctgat gtttcccggc ctgttcatct ttgggcctga aggcatgcac accctgagcg
     1441 tggctgggtc cactattggc cataccatcg ccgacctgct gtccaagaat atttggggac
     1501 tgctggggca cttcctgcgg atcaaaattc acgagcatat cattatgtac ggcgatatca
     1561 ggagaccagt gagctcccag tttctgggac gcaaggtgga cgtgctggcc ttcgtgacag
     1621 aggaagataa agtggcggcc gccaagagca ggatcaccag cgagggcgag tacatccccc
     1681 tggaccagat cgacatcaac gtggtgagca agggcgagga gctgttcacc ggggtggtgc
     1741 ccatcctggt cgagctggac ggcgacgtaa acggccacag gttcagcgtg tccggcgagg
     1801 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1861 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
     1921 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1981 tccaggagcg taccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2041 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2101 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
     2161 ccgccgacaa gcagaagaac ggcatcaagg cccacttcaa gatccgccac aacatcgagg
     2221 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2281 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2341 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2401 tggacgagct gtacaagtaa ttagctagct aaacaacttc gtatatacct ttctatacga
     2461 agttgtcgaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     2521 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2581 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2641 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2701 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2761 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2821 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2881 ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg
     2941 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3001 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3061 cctttgggcc gcctccccgc atcgataccg agcgctgctc gagctagagc tcgctgatca
     3121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3301 gaggattggg aagacaatag caggcatgct ggggaggtaa ccacgtgcgg accgagcggc
     3361 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3421 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3481 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3541 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     3601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3781 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3901 aacactcaat cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     3961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4021 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4081 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4141 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4201 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4261 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4441 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4501 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4561 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4621 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4681 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4741 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4801 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4861 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4921 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4981 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5041 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5101 gtttattgct gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact
     5161 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5581 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6061 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
