LOCUS       LZF1604V2_pCAG-A       10096 bp    DNA     circular SYN 13-FEB-2017
DEFINITION  pCAG-FLEX-fwd[Chrimson-tdT].
ACCESSION   urn.local...e-77q3s0p
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
            .
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="pCAG-FLEX-fwd[Chrimson-tdT]"
                     /mol_type="other DNA"
     misc_feature    84..371
                     /label="CAG_enhancer"
     CDS             complement(739..1347)
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                     HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
                     QRALPEPFKAFTQPQKRNEPSLNQALITARRRRAVPPAHLWE*"
                     /label="ORF frame 2"
     misc_feature    1682..1701
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                     HGEPRATPRTEPRTRSRAVLSNSGRGVQGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPAPRGTAPGRAPLAHTAERTKPRAAPAALTAAGQRGAARGAPHAHTHARTPRAAPPA
                     QRALPEPFKAFTQPQKRNEPSLNQALITARRRRAVPPAHLWE*"
                     /label="pCAG_F_primer"
     misc_feature    1699..1724
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF1604OL_F_71"
     misc_feature    1700..1751
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF1604V2_F_72"
     misc_feature    1723..1728
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     source          complement(<1729..>1749)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1729..1740
                     /note="ZFBS"
                     /modified_by="User"
     misc_feature    1741..1749
                     /note="Kozak Sequence"
                     /modified_by="User"
     misc_feature    1750..>3159
                     /ApEinfo_fwdcolor="#804040"
                     /ApEinfo_revcolor="#804040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="jRGECO1a"
     misc_feature    1753..3095
                     /Original_Bases="GTCGACTCATCACGTCGTAAGTGGAATAAGACAGGTCACGC
                     AGTCAGAGCTATAGGTCGGCTGAGCTCACTCGAGAACGTCTATATCAAGGCCGACAAG
                     CAGAAGAACGGCATCAAGGCGAACTTCCACATCCGCCACAACATCGAGGACGGCGGCG
                     TGCAGCTCGCCTACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCT
                     GCCCGACAACCACTACCTGAGCGTGCAGTCCAAACTTTCGAAAGACCCCAACGAGAAG
                     CGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGG
                     ACGAGCTGTACAAGGGCGGTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGAGCTGTT
                     CACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTC
                     AGCGTGTCCGGCGAGGGTGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCA
                     TCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
                     CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAG
                     TCCGCCATGCCCGAAGGCTACATCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCA
                     ACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGA
                     GCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTAC
                     AACCTGCCGGACCAACTGACTGAAGAGCAGATCGCAGAATTTAAAGAGGCTTTCTCCC
                     TATTTGACAAGGACGGGGATGGGACAATAACAACCAAGGAGCTGGGGACGGTGATGCG
                     GTCTCTGGGGCAGAACCCCACAGAAGCAGAGCTGCAGGACATGATCAATGAAGTAGAT
                     GCCGACGGTGACGGCACAATCGACTTCCCTGAGTTCCTGACAATGATGGCAAGAAAAA
                     TGAAATACAGGGACACGGAAGAAGAAATTAGAGAAGCGTTCGGTGTGTTTGATAAGGA
                     TGGCAATGGCTACATCAGTGCAGCAGAGCTTCGCCACGTGATGACAAACCTTGGAGAG
                     AAGTTAACAGATGAAGAGGTTGATGAAATGATCAGGGA"
                     /label="GTCGACTCATCACGTCGTAAGTGGAATAAGACAGGTCACGCAGTCAGAGC
                     TATAGGTCGGCTGAGCTCACTCGAGAACGTCTATATCAAGGCCGACAAGCAGAAGAAC
                     GGCATCAAGGCGAACTTCCACATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCG
                     CCTACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAA
                     CCACTACCTGAGCGTGCAGTCCAAACTTTCGAAAGACCCCAACGAGAAGCGCGATCAC
                     ATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGT
                     ACAAGGGCGGTACCGGAGGGAGCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGT
                     GGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCC
                     GGCGAGGGTGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCA
                     CCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCA
                     GTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATG
                     CCCGAAGGCTACATCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGA
                     CCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGG
                     CATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACCTGCCG
                     GACCAACTGACTGAAGAGCAGATCGCAGAATTTAAAGAGGCTTTCTCCCTATTTGACA
                     AGGACGGGGATGGGACAATAACAACCAAGGAGCTGGGGACGGTGATGCGGTCTCTGGG
                     GCAGAACCCCACAGAAGCAGAGCTGCAGGACATGATCAATGAAGTAGATGCCGACGGT
                     GACGGCACAATCGACTTCCCTGAGTTCCTGACAATGATGGCAAGAAAAATGAAATACA
                     GGGACACGGAAGAAGAAATTAGAGAAGCGTTCGGTGTGTTTGATAAGGATGGCAATGG
                     CTACATCAGTGCAGCAGAGCTTCGCCACGTGATGACAAACCTTGGAGAGAAGTTAACA
                     GATGAAGAGGTTGATGAAATGATCAGGGA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2894..2911
                     /created_by="User"
                     /label="95_sp13_onemismatch"
     misc_feature    complement(3135..3159)
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF96_R1_65"
     misc_feature    3135..3159
                     /created_by="User"
                     /label="LZF96_F2"
     source          complement(<3160..>4851)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(3160..3207)
                     /note="4GGGS"
     misc_feature    complement(3214..3636)
                     /note="TPR3"
     misc_feature    complement(3643..4422)
                     /note="Utrophin1-783nt"
     misc_feature    3643..3659
                     /created_by="User"
                     /label="LZFsp14"
     misc_feature    4323..4328
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4429..4851)
                     /note="TPR3"
     source          complement(<4852..>5205)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4855..4860
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4858..5205)
                     /note="ZFn (CCR5-L)"
     misc_feature    complement(4860..4883)
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF1604V2_R"
     misc_feature    4868..4889
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF1603_F1_70"
     misc_feature    complement(5187..5261)
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF1603_R1_66"
     misc_feature    5206..5271
                     /Color="#ff77ff"
                     /created_by="User"
                     /label="P2A"
     misc_feature    5238..>5290
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF1603_F2_63"
     CDS             join(5272..5655,5659..5782,5784..6039)
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="QuasAr2"
     misc_feature    5679
                     /created_by="User"
                     /modified_by="User"
                     /label="c_a mut"
     misc_feature    5685
                     /created_by="User"
                     /label="c to t"
     CDS             6040..6099
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TS"
     CDS             6100..6810
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Citrine"
     misc_feature    complement(6280..6311)
                     /created_by="User"
                     /label="MP9"
     misc_feature    6280..6311
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF70_Mutagenesis_MP11"
     misc_feature    complement(6341..6360)
                     /created_by="User"
                     /modified_by="User"
                     /label="LZFsp03"
     CDS             6811..7008
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TSx3"
     misc_feature    complement(7008..7063)
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF1604_R_63"
     CDS             7009..7029
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ER2"
     misc_feature    7033..7040
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(7043..7063)
                     /created_by="User"
                     /modified_by="User"
                     /label="LZF1604OL_R_71"
     terminator      7050..7564
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="rb_glob_PA_terminator"
     promoter        complement(7595..7613)
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="M13_reverse_primer"
     misc_feature    complement(7612..7634)
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="M13_pUC_rev_primer"
     promoter        complement(7648..7677)
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="lac_promoter"
     promoter        7783..7962
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="SV40_promoter"
     rep_origin      7818..7895
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="SV40_origin"
     misc_feature    7880..7899
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="SV40pro_F_primer"
     terminator      7976..8107
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="SV40_PA_terminator"
     misc_feature    8064..8083
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="EBV_rev_primer"
     gene            complement(9105..9965)
                     /gene="Ampicillin"
                     /translation="MVASFRPLILFHNGVVFIVGCDIQLGVHIVIAGQLHWFLGHVDG
                     FELHQVVAAVFQLQGLVDFALQHSVSRIQPFSAGLPPHCFLLHHGPIGWKIGAAHFDF
                     VDQSAILQRGVLCHGHQASIFKIHHSLPLKALWERQFLVIRNVSGMLDIGLTSVHKLR
                     RQDIPSERQWSSLGHFQLGSLCALVRPPFALSFNLKFVPIHAALHAHLKPHELFDHSH
                     IVILA*"
                     /label="Ampicillin"
     CDS             complement(9105..9965)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 3"
     promoter        complement(10007..10035)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc
      841 cttaaagggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgt catcctcatc
     1741 gccgccacca tgctgcagaa cgagcttgct cttaagttgg ctggacttga tattaacaag
     1801 actggaggag gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
     1861 caaatgggtc gggatctgta cgacgatgac gataaggatc tcgcaacaat ggtcgactca
     1921 tcacgtcgta agtggaataa ggcaggtcac gcagtcagag ctataggtcg gctgagctca
     1981 cccgtggttt ccgagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag
     2041 gggctgaggc tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc
     2101 aagaagcccg tgcagctgcc cggcgcctac atcgtggaca tcaagttgga catcgtgtcc
     2161 cacaacgagg actacaccat cgtggaacag tgcgaacgcg ccgagggccg ccactccacc
     2221 ggcggcatgg acgagctgta caagggaggt acaggcggga gtctggtgag caagggcgag
     2281 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2341 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt
     2401 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2461 tcccctcagt tcatgtacgg ctccaaggcc tacattaagc acccagccga catccccgac
     2521 tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac
     2581 ggcggcatta ttcacgtgaa ccaggactcc tccctgcagg acggcgtatt catctacaag
     2641 gtgaagctgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     2701 ggctgggagg ctacgcgtga cgacctgact gaagagcaga tcgcagaatt taaagaggct
     2761 ttctccctat ttgacaagga cggggatggg acgataacaa ccaaggagct tgggacggtg
     2821 ttccgatcgc tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta
     2881 gatgccgacg gtgacggcac attcgacttc cctgagttcc tgacgatgat ggcaagaaaa
     2941 atgaatgaca cagacagtga agaggaaatt cgcgaagcgt tccgcgtgtt tgataaggac
     3001 ggcaatggct acatcggcgc agcagagctt cgccacgtga tgacagacct tggagagaag
     3061 ttaacagatg aggaggttga tgaaatgatc agggtagcag acatcgatgg ggatggtcag
     3121 gtaaactacg aagagtttgt acaaatgatg acagcgaagg gtggaggttc tggtggaggt
     3181 tctggaggtg gatcaggagg aggttcgaga tctaagctct caggtggcgg agggagcggc
     3241 ggaggtggct ctggaggtgg tggctcagct gaagcatggt acaatctggg aaacgcttat
     3301 tacaagcagg gcgactatca aaaagccatt gagtattacc aaaaggctct tgagctagac
     3361 cctaataacg ccgaagcgtg gtataaccta ggcaacgcat attacaagca gggagactac
     3421 caaaaggcaa tcgaatatta ccagaaggca ctcgaactgg acccaaataa cgcagaggct
     3481 tggtataatc tgggaaacgc ctactataaa cagggtgact accaaaaggc gatagaggac
     3541 tatcagaagg ccttggagct cgatccgaac aatctgcaac gtagtgctgg tggcggaggg
     3601 tctggtggag gaggctcagg aggaggtggc gcatcaaacg ttgccaagta cgggcacctt
     3661 gaagccagcc ctgatgatgg gcagaatcag ttcagtgata tcattaagtc cagatctgat
     3721 gaacacaacg atgtacagaa gaaaaccttt accaaatgga taaacgctcg attttcaaag
     3781 agtgggaaac cacccatcaa tgatatgttc tcagacctca aagatgggag aaagctcttg
     3841 gatcttctag aaggcctcac aggaacatca ttgccaaagg aacgtggttc cacaagggtg
     3901 catgccttaa ataatgtcaa ccgggtgctg caggttttac atcagaacaa tgtggagctg
     3961 gtgaatatcg gaggcactga cattgtggat ggaaatccca agctgacgct gggtttgctg
     4021 tggagtatta ttctgcactg gcaggtgaag gatgtcatga aagatatcat gtcagacctg
     4081 cagcagacga acagcgagaa gatcctgctg agctgggtac ggcagaccac caggccctac
     4141 agccaagtca acgtcctcaa cttcaccacc agctggacag atggactcgc attcaacgcc
     4201 gtgctccacc ggcacaaacc agatctcttc agctgggaca gagtggtcaa aatgtcccca
     4261 actgagagac ttgaacatgc ttttagcaag gcccatactt atttggggat tgaaaaactt
     4321 ctggatcctg aagatgttgc cgtgcagctc cctgacaaga aatccataat tatgtattta
     4381 acgtctctgt ttgaggtgct tcctcagcaa gtcactatag accaattgaa gctctcaggt
     4441 ggcggaggga gcggcggagg tggctctgga ggtggtggct cagctgaagc atggtacaat
     4501 ctgggaaacg cttattacaa gcagggcgac tatcaaaaag ccattgagta ttaccaaaag
     4561 gctcttgagc tagaccctaa taacgccgaa gcgtggtata acctaggcaa cgcatattac
     4621 aagcagggag actaccaaaa ggcaatcgaa tattaccaga aggcactcga actggaccca
     4681 aataacgcag aggcttggta taatctggga aacgcctact ataaacaggg tgactaccaa
     4741 aaggcgatag aggactatca gaaggccttg gagctcgatc cgaacaatct gcaacgtagt
     4801 gctggtggcg gagggtctgg tggaggaggc tcaggaggag gtggcgcatc aaacgaattc
     4861 cagtgccgga tctgcatgcg gaacttcagc gaccggtcca acctgagcag gcacatcaga
     4921 acccacaccg gagaaaagcc cttcgcctgc gacatttgcg gccggaagtt cgccatcagc
     4981 agcaacctga acagccacac caagatccac actggcagcc agaaaccttt ccagtgcaga
     5041 atttgtatga gaaactttag cagaagcgac aacctggcca gacacatccg gacacatact
     5101 ggtgaaaaac cttttgcctg tgatatctgt ggcagaaagt ttgccacctc cggcaatctg
     5161 acccggcaca caaagattca cctgcggggc agccagctat cgattggctc cggagccacg
     5221 aacttctctc tgttaaagca agcaggagac gtggaagaaa accccggtcc catggtaagt
     5281 atcgctctgc aggctggtta cgacctactg ggtgacggca gacctgaaac cctgtggctg
     5341 ggcatcggca ctctgctgat gctgattgga accttctact ttctggtccg cggatgggga
     5401 gtcaccgata aggatgcccg ggaatattac gctgtgacta tcctggtgtc cggaatcgca
     5461 tccgccgcat atctgtctat gttctttggt atcgggctta ctgaggtgtc cgtcgggggc
     5521 gaaatgttgg atatctatta tgccaggtac gcccagtggc tgtttaccac cccacttctg
     5581 ctgctgcacc tggcccttct cgctaaggtg gatcgggtga ccatcggcac cctggtgggt
     5641 gtggacgccc tgatgatcgt cactggcctc atcggagcct tgagccacac ggccatagcc
     5701 agatacagtt ggtggttgtt ctctacaatt tgcatgatag tggtgctcta tgttctggct
     5761 acatccctgc gatctgctgc aagggagcgg ggccccgagg tggcatctac ctttaacacc
     5821 ctgacagctc tggtcttggt gctgtggacc gcttacccta tcctgtggat cataggcact
     5881 gagggcgctg gcgtggtggg cctgggcatc gaaactctgc tgtttatggt gttggacgtg
     5941 actgccaagg tcggctttgg ctttatcctg ttgagatccc gggctattct gggcgacacc
     6001 gaggcaccag aacccagtgc cggtgccgat acgttgatga agagtagaat cacaagcgaa
     6061 ggcgagtaca tccccctgga tcaaatagac ataaatgtag tgagcaaggg cgaggagctg
     6121 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6181 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     6241 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggcggcggc
     6301 ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6361 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     6421 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6481 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6541 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     6601 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6661 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     6721 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6781 gggatcactc tcggcatgga cgagctgtac aaatcaagga tcacctctga gggcgagtat
     6841 atccctctgg atcagatcga cattaacgtc ggtggaaaat cccgcataac ctctgagggc
     6901 gagtacatcc ctctggatca aatcgatata aacgtggggg gaaaaagtcg aatcacttct
     6961 gagggcgagt atatacccct cgatcagatt gacatcaatg tggggggctt ttgttatgag
     7021 aatgaagtgt aggcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     7081 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     7141 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     7201 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     7261 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     7321 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     7381 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     7441 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     7501 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     7561 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     7621 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7681 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7741 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     7801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7921 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt
     7981 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8041 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8101 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8161 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8221 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8281 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8461 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8521 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8581 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8641 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8701 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8761 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8821 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8881 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8941 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9001 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9061 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9121 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9181 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9241 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9301 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9361 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9421 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9481 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9541 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9601 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9661 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9721 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9781 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9841 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9901 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9961 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10021 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10081 cgaaaagtgc cacctg
//
