LOCUS       2A_trigger_combi        9706 bp ds-DNA     circular     21-NOV-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    2A trigger combined with bikard dCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 18 Nov 2016 from SnapGene 1.1.3 http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(220..322)
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
                     /label=cat_promoter
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    701..706
                     /note="EcoRI"
                     /note="color: #ff99cc"
                     /label=EcoRI
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    707..760
                     /note="PLtet-O12"
                     /note="color: #19ff1a; direction: RIGHT"
                     /label=PLtet-O12
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    707..725
                     /note="tet operator"
                     /note="color: #31849b"
                     /label=tet operator
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    732..750
                     /note="tet operator"
                     /note="color: #31849b"
                     /label=tet operator(1)
                     /ApEinfo_label="tet operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    761..775
                     /note="VR2'"
                     /note="color: #00ffff"
                     /label=VR2'
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    785..799
                     /note="VR1'"
                     /note="color: #ff00ff"
                     /label=VR1'
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      800..828
                     /note="rrnC terminator"
                     /note="color: #ff1f19; direction: RIGHT"
                     /label=rrnC terminator
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    829..834
                     /note="PstI"
                     /note="color: #ff99cc"
                     /label=PstI
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1228..1773)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E.
                     coli cells that contain a second plasmid with the colE1
                     origin."
                     /note="color: #ffff00"
                     /label=p15A_ori
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1885..1913
                     /note="tet promoter"
                     /note="promoter for tetracycline efflux protein gene"
                     /note="This forward directional feature has 3 segments:
                        1: 1885 .. 1890 / #ffffff / -35
                        2: 1891 .. 1907 / #ffffff
                        3: 1908 .. 1913 / #ffffff / -10"
                     /label=tet promoter
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1961..2062
                     /codon_start=1
                     /note="partial tet resistance"
                     /note="color: #993366"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRD"
                     /label=partial tet resistance
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2224..2264)
                     /note="S. pyogenes Terminator"
                     /note="color: #a6acb3; direction: LEFT"
                     /label=S_pyogenes_terminato
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2226..2312
                     /note="TracrRNA"
                     /note="color: #59ff27"
                     /label=TracrRNA (guide)
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(2330..2359)
                     /note="tracrRNA promoter?"
                     /note="This reverse directional feature has 3 segments:
                        1: 2330 .. 2335 / #ffffff / -10
                        2: 2336 .. 2353 / #ffffff
                        3: 2354 .. 2359 / #ffffff / -35"
                     /label=tracrRNA promoter?
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2547..2577
                     /note="dCas9 promoter"
                     /note="This forward directional feature has 3 segments:
                        1: 2547 .. 2552 / #ffffff / -35
                        2: 2553 .. 2571 / #ffffff
                        3: 2572 .. 2577 / #ffffff / -10"
                     /label=dCas9_promoter
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2594..2599
                     /note="rbs"
                     /note="color: #38ff49"
                     /label=rbs
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2605..6711
                     /codon_start=1
                     /note="dCas9"
                     /note="color: #00ffff"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     K
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     S
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMI
                     K
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRR
                     L
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLA
                     Q
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALV
                     R
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDL
                     L
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
                     G
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYE
                     Y
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECF
                     D
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEE
                     R
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNF
                     M
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMG
                     R
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKL
                     Y
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNV
                     P
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITK
                     H
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAY
                     L
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFK
                     T
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFS
                     K
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLG
                     I
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGN
                     E
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILA
                     D
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEV
                     L DATLIHQSITGLYETRIDLSQLGGD"
                     /label=dCas9
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2633..2633
                     /note="A->C"
                     /note="First mutation to create dCas9, Aspartic acid to
                     Alanine"
                     /note="color: #ff0000"
                     /label=A->C
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5122..5123
                     /note="CA->GC"
                     /note="Second mutation to create dCas9, Histidine to
                     Alanine."
                     /note="color: #ff0000"
                     /label=CA->GC
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6864..6900
                     /note="Direct Repeat"
                     /note="color: #d62bff"
                     /label=Direct Repeat(1)
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6901..6906
                     /note="BsaI"
                     /note="color: #ff9f23"
                     /label=BsaI
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6924..6929
                     /note="BsaI"
                     /note="color: #ff8908"
                     /label=BsaI(1)
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6930..6966
                     /note="Direct Repeat"
                     /note="color: #f641ff"
                     /label=Direct Repeat
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7204..8232
                     /codon_start=1
                     /note="TetA inactive"
                     /note="color: #993366"
                     /translation="MQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWI
                     L
                     YAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAI
                     S
                     LHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALM
                     T
                     VFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFG
                     E
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     Q
                     LQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRA
                     T ST"
                     /label=TetA inactive
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(join(9266..9706,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     L
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFS
                     S
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVAN
                     M
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
                     /label=cmR_chloramphenicol
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acagcttgat tctagctgat cgtggaccgg aaggtgagcc agtgagttga ttgcagtcca
      601 gttacgctgg agtctgaggc tcgtcctgaa tgatatgcga ccgccggagg gttgcgtttg
      661 agacgggcga cagatcgaca ctgctcgatc cgctcgcacc gaattctccc tatcagtgat
      721 agagattgac atccctatca gtgatagaga tactgagcac tcattaacca tacccagggt
      781 attccggact tcttcaaaca tccttagcga aagctaagga ttttttttct gcagggatcg
      841 acgagagcag cgcgactgga tcagttctgg acgagcgagc tgtcgtccga cccgtgatct
      901 tacggcatta tacgtatgat cgggggtggt gcgtaacggc aaaagcaccg ccggacatca
      961 gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
     1021 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
     1081 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     1141 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
     1201 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
     1261 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     1321 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     1381 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     1441 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     1501 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     1561 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     1621 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     1681 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     1741 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
     1801 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
     1861 cgatataagt tgtaattctc atgtttgaca gcttatcatc gataagcttt aatgcggtag
     1921 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct
     1981 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt
     2041 actgccgggc ctcttgcggg attacgaaat catcctgtgg agcttagtag gtttagcaag
     2101 atggcagcgc ctaaatgtag aatgataaaa ggattaagag attaatttcc ctaaaaatga
     2161 taaaacaagc gttttgaaag cgcttgtttt tttggtttgc agtcagagta gaatagaagt
     2221 atcaaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt
     2281 taacttgcta tgctgttttg aatggttcca acaagattat tttataactt ttataacaaa
     2341 taatcaagga gaaattcaaa gaaatttatc agccataaaa caatacttaa tactatagaa
     2401 tgataacaaa ataaactact ttttaaaaga attttgtgtt ataatctatt tattattaag
     2461 tattgggtaa tattttttga agagatattt tgaaaaagaa aaattaaagc atattaaact
     2521 aatttcggag gtcattaaaa ctattattga aatcatcaaa ctcattatgg atttaattta
     2581 aactttttat tttaggaggc aaaaatggat aagaaatact caataggctt agctatcggc
     2641 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc
     2701 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta
     2761 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat
     2821 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa
     2881 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag
     2941 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat
     3001 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc
     3061 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga
     3121 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac
     3181 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt
     3241 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag
     3301 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt
     3361 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat
     3421 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca
     3481 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata
     3541 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg
     3601 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt
     3661 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt
     3721 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa
     3781 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat
     3841 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt
     3901 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt
     3961 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca
     4021 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt
     4081 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt
     4141 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa
     4201 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc
     4261 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata
     4321 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt
     4381 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat
     4441 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt
     4501 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa
     4561 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat
     4621 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat
     4681 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa
     4741 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct
     4801 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg
     4861 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag
     4921 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga
     4981 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat
     5041 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt
     5101 ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata
     5161 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt
     5221 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc
     5281 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat
     5341 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca
     5401 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag
     5461 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc
     5521 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc
     5581 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat
     5641 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca
     5701 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt
     5761 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt
     5821 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc
     5881 aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca
     5941 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt
     6001 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg
     6061 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt
     6121 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa
     6181 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg
     6241 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat
     6301 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac
     6361 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa
     6421 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt
     6481 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta
     6541 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat
     6601 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc
     6661 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgactg aagtatattt
     6721 tagatgaaga ttatttctta ataactaaaa atatggtata atactcttaa taaatgcagt
     6781 aatacagggg cttttcaaga ctgaagtcta gctgagacaa atagtgcgat tacgaaattt
     6841 tttagacaaa aatagtctac gaggttttag agctatgctg ttttgaatgg tcccaaaact
     6901 gagaccagtc tcggaagctc aaaggtctcg ttttagagct atgctgtttt gaatggtccc
     6961 aaaacttcag cacactgaga cttgttgagt tccatgtttt agagctatgc tgttttgaat
     7021 ggactccatt caacattgcc gatgataact tgagaaagag ggttaatacc agcagtcgga
     7081 taccttccta ttctttctgt taaagcgttt tcatgttata ataggcaaaa gaagagtagt
     7141 gtgatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     7201 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     7261 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     7321 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     7381 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     7441 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     7501 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     7561 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
     7621 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     7681 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     7741 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     7801 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     7861 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     7921 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     7981 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     8041 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     8101 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     8161 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     8221 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     8281 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat
     8341 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct
     8401 ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     8461 gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     8521 gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     8581 gtctggaaac gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac
     8641 caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt
     8701 attctgagtt acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca
     8761 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     8821 cggcagcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     8881 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     8941 cacctacatc tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata
     9001 aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc
     9061 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     9121 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     9181 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag
     9241 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
     9301 gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
     9361 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     9421 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     9481 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     9541 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     9601 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     9661 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacg 
//
