LOCUS       pRS52                   4295 bp ds-DNA     circular     15-OCT-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    3326..3345
                     /label="OmpR-F2"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    complement(2070..2071)
                     /label="GC>CA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    3306..3325
                     /label="OmpR-F1"
                     /ApEinfo_revcolor=#f9ad81
                     /ApEinfo_fwdcolor=#f9ad81
     misc_feature    3208..3245
                     /label="BW25113 LacZ Upstream"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc            complement(2016..2032)
                     /label="inverted repeat"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3334..3339
                     /label="-35 region(2)"
                     /ApEinfo_revcolor=#f6989d
                     /ApEinfo_fwdcolor=#f6989d
     misc_feature    complement(1364..1369)
                     /label="-35"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc            complement(1982..2005)
                     /label="inverted repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          3175..3194
                     /label="Sequencing Primer SR126"
                     /ApEinfo_revcolor=#fdc68a
                     /ApEinfo_fwdcolor=#fdc68a
     misc_feature    1313..1359
                     /label="Inverted Repeat"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3295..3440
                     /label="PompF"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4167..4246
                     /label="B0010"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    160..2060
                     /label="pSC101 Fragment"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        3354..3359
                     /label="-35 region(1)"
                     /ApEinfo_revcolor=#6ecff6
                     /ApEinfo_fwdcolor=#6ecff6
     misc_feature    complement(1409..1428)
                     /label="Direct Repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1480..1562
                     /label="AT Rich Region"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        3357..3362
                     /label="-10 region(2)"
                     /ApEinfo_revcolor=#c4df9b
                     /ApEinfo_fwdcolor=#c4df9b
     Terminator      complement(2066..2171)
                     /label="T0"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    3346..3365
                     /label="OmpR-F3"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc            complement(1959..1976)
                     /label="inverted repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        3382..3387
                     /label="-10 region(1)"
                     /ApEinfo_revcolor=#bc8dbf
                     /ApEinfo_fwdcolor=#bc8dbf
     misc_feature    complement(3249..3292)
                     /label="trpT tRNA Terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             3441..4160
                     /label="superfolder GFP (RBS 7.125)"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    2197..3245
                     /label="pUC19 fragment"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc            complement(1693..2060)
                     /label="par region"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      4167..4295
                     /label="B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    complement(1441..1461)
                     /label="Direct Repeat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        3406..3406
                     /label="+1 nucleotide"
                     /ApEinfo_revcolor=#82ca9d
                     /ApEinfo_fwdcolor=#82ca9d
     misc_feature    4247..4254
                     /label="BB Scar"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1342..1347)
                     /label="-10"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(2197..2859)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        3371..3376
                     /label="-35 region"
                     /ApEinfo_revcolor=#6ecff6
                     /ApEinfo_fwdcolor=#6ecff6
     misc            complement(2045..2058)
                     /label="inverted repeat"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1462..1482)
                     /label="Direct Repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(361..1311)
                     /label="Rep"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    7..70
                     /label="E. coli His Terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        3394..3399
                     /label="-10 region"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    1359..1385
                     /label="Inverted Repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        3308..3313
                     /label="-10 region(3)"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc            complement(2035..2039)
                     /label="Tn3 integration site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4255..4295
                     /label="B0012"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1380..1381
                     /label="ta>at removes SpeI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
       61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      121 gcgagcggta tcagctcact caaaggcggt aatctcgagg ttacattgtc gatctgttca
      181 tggtgaacag ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt
      241 acaccgtttt catctgtgca tatggacagt tttccctttg atatctaacg gtgaacagtt
      301 gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc
      361 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
      421 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      481 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      541 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      601 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      661 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      721 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      781 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      841 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      901 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      961 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
     1021 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
     1081 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
     1141 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
     1201 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
     1261 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
     1321 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattaca
     1381 tgtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1441 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1501 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1561 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1621 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1681 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1741 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1801 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1861 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1921 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1981 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     2041 ggcttacccg tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg
     2101 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2161 aggagtccaa gcgagctcgt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2221 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2281 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2341 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2401 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2461 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2521 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2581 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2641 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2701 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2761 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2821 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2881 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2941 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3001 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3061 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3121 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc ccatggtgcc
     3181 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3241 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggttcct
     3301 taaattttac ttttggttac atattttttc tttttgaaac caaatcttta tctttgtagc
     3361 actttcacgg tagcgaaacg ttagtttgaa tggaaagatg cctgcaggtg tcataaaaaa
     3421 aaccatgagg gtaataaata atgcgtaaag gcgaagagct gttcactggt gtcgtcccta
     3481 ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg
     3541 aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc
     3601 cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt
     3661 atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc
     3721 aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat
     3781 ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg
     3841 gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg
     3901 ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg
     3961 gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc
     4021 tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga
     4081 aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg
     4141 atgaactgta caaatgatga agatctCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA
     4201 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCTACT AGAGTCACAC
     4261 TGGCTCACCT TCGGGTGGGC CTTTCTGCGT TTATA
//