LOCUS       pSR26-2                 3624 bp ds-DNA     circular     15-OCT-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(735..740)
                     /label="-35"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    580..586
                     /label="-10"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    557..562
                     /label="-35"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(151..703)
                     /label="RNA II"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    593..700
                     /label="RNAI"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(133..815)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(763..815)
                     /label="Tn3 Artifact"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2730..2737
                     /label="BB Scar"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      2650..2778
                     /label="B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    complement(927..932)
                     /label="SacI Scar"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1851..1856
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2893..3519
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(942..1601)
                     /label="CmR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1863..1882
                     /label="Lambda Phage Fragment"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1883..1917
                     /label="sRBS (500)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Terminator      complement(820..925)
                     /label="T0"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1809..1827
                     /label="TetR1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1863..1863
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1834..1852
                     /label="TetR2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1828..1833
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1809..1862
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1936..2265
                     /label="Phosphoacceptor domain"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2779..2823
                     /label="pUC MCS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2650..2729
                     /label="B0010"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2284..2328
                     /label="Linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        2824..2858
                     /label="J23106"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1803..1808
                     /label="XhoI Scar"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2859..2892
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1918..2637
                     /label="ompR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(711..717)
                     /label="-10"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2386..2616
                     /label="DNA-binding domain"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1..6)
                     /label="AvrII Scar"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1751..1801
                     /label="BW25113 LacZ Upstream"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      3520..3624
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    complement(927..1721)
                     /label="CamR Cassette from E. coli R6-5 plasmid"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(815..820)
                     /label="SpeI Scar"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(6..819)
                     /label="ColE1 Fragment"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2738..2778
                     /label="B0012"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 CCTAGGCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
       61 CACAGAATCA GGGGATAACG CATGCAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
      121 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      181 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      241 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      301 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
      361 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      421 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      481 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      541 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
      601 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      661 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
      721 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
      781 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGACTAGt gcttggattc tcaccaataa
      841 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      901 ctggatctat caacaggagt ccaagCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC
      961 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG
     1021 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
     1081 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
     1141 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
     1201 AAATAGGCCA GGTTTTCACC ATAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
     1261 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
     1321 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
     1381 CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
     1441 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
     1501 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
     1561 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
     1621 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
     1681 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA
     1741 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC
     1801 ACCTCGAGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
     1861 ACATCAGCAG GACGCACTGA CCGCATAAAG GACTTAGCAA AGCCCAATTT TAAACAAATG
     1921 CAAGAGAACT ACAAGATTCT GGTGGTCGAT GACGACATGC GCCTGCGTGC GCTGCTGGAA
     1981 CGTTATCTCA CCGAACAAGG CTTCCAGGTT CGAAGCGTCG CTAATGCAGA ACAGATGGAT
     2041 CGCCTGCTGA CTCGTGAATC TTTCCATCTT ATGGTACTGG ATTTAATGTT ACCTGGTGAA
     2101 GATGGCTTGT CGATTTGCCG ACGTCTTCGT AGTCAGAGCA ACCCGATGCC GATCATTATG
     2161 GTGACGGCGA AAGGGGAAGA AGTGGACCGT ATCGTAGGCC TGGAGATTGG CGCTGACGAC
     2221 TACATTCCAA AACCGTTTAA CCCGCGTGAA CTGCTGGCCC GTATCCGTGC GGTGCTGCGT
     2281 CGTCAGGCGA ACGAACTGCC AGGCGCACCG TCACAGGAAG AGGCGGTAAT TGCTTTCGGT
     2341 AAGTTCAAAC TTAACCTCGG TACGCGCGAA ATGTTCCGCG AAGACGAGCC GATGCCGCTC
     2401 ACCAGCGGTG AGTTTGCGGT ACTGAAGGCA CTGGTCAGCC ATCCGCGTGA GCCGCTCTCC
     2461 CGCGATAAGC TGATGAACCT TGCCCGTGGT CGTGAATATT CCGCAATGGA ACGCTCCATC
     2521 GACGTGCAGA TTTCGCGTCT GCGCCGCATG GTGGAAGAAG ATCCAGCGCA TCCGCGTTAC
     2581 ATTCAGACCG TCTGGGGTCT GGGCTACGTC TTTGTACCGG ACGGCTCTAA AGCATGATAA
     2641 TAATCTAGAC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT
     2701 TTATCTGTTG TTTGTCGGTG AACGCTCTCT ACTAGAGTCA CACTGGCTCA CCTTCGGGTG
     2761 GGCCTTTCTG CGTTTATAGG TACCCGTGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG
     2821 CTTtttacgg ctagctcagt cctaggtata gtgctagcCC AGCCAGAGAA ACACTCTTTA
     2881 ACAGGGGGTA GTATGATGTC TCGTTTAGAT AAAAGTAAAG TGATTAACAG CGCATTAGAG
     2941 CTGCTTAATG AGGTCGGAAT CGAAGGTTTA ACAACCCGTA AACTCGCCCA GAAGCTAGGT
     3001 GTAGAGCAGC CTACATTGTA TTGGCATGTA AAAAATAAGC GGGCTTTGCT CGACGCCTTA
     3061 GCCATTGAGA TGTTAGATAG GCACCATACT CACTTTTGCC CTTTAGAAGG GGAAAGCTGG
     3121 CAAGATTTTT TACGTAATAA CGCTAAAAGT TTTAGATGTG CTTTACTAAG TCATCGCGAT
     3181 GGAGCAAAAG TACATTTAGG TACACGGCCT ACAGAAAAAC AGTATGAAAC TCTCGAAAAT
     3241 CAATTAGCCT TTTTATGCCA ACAAGGTTTT TCACTAGAGA ATGCATTATA TGCACTCAGC
     3301 GCAGTGGGGC ATTTTACTTT AGGTTGCGTA TTGGAAGATC AAGAGCATCA AGTCGCTAAA
     3361 GAAGAAAGGG AAACACCTAC TACTGATAGT ATGCCGCCAT TATTACGACA AGCTATCGAA
     3421 TTATTTGATC ACCAAGGTGC AGAGCCAGCC TTCTTATTCG GCCTTGAATT GATCATATGC
     3481 GGATTAGAAA AACAACTTAA ATGTGAAAGT GGGTCTTAAG GCATCAAATA AAACGAAAGG
     3541 CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA
     3601 GTAGGACAAA TCCGCCGCCC TAGA
//