LOCUS       Exported               10294 bp ds-DNA     circular SYN 16-JUL-2017
DEFINITION  lentiGuide-Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2017 from SnapGene 4.0.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        87..1265
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        94..1265
                     /label=EF1a_promoter
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     intron          318..1256
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     CDS             complement(385..1005)
                     /codon_start=1
                     /label=ORF frame 2
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPLT
                     QLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGPRR
                     RPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFARGGP
                     SAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK"
     misc_feature    1213..1233
                     /label=EF1a_fwd_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     CDS             1278..1988
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    join(1278..1708,1710..1988)
                     /label=mCherry
                     /note="ApEinfo_fwdcolor=#ff8080
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="This feature has 2 segments:
                        1: 1278 .. 1708 / #ff8080
                        2: 1710 .. 1988 / #ff8080"
     misc_feature    1278..1703
                     /label=reading frame
                     /note="ApEinfo_fwdcolor=#fff78a
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #fff78a"
     misc_feature    join(1706..1708,1710..1711)
                     /label=bbs1
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="This feature has 2 segments:
                        1: 1706 .. 1708 / #00ffff
                        2: 1710 .. 1711 / #00ffff"
     misc_feature    2004..2592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    2006..2581
                     /label=WPRE
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     CDS             2094..2603
                     /codon_start=1
                     /label=ORF frame 3
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFA
                     DATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKSS
                     SFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWA
                     ASPRRL"
     CDS             2107..2637
                     /codon_start=1
                     /label=ORF frame 1
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPP
                     LVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLG
                     CSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRV
                     DFKTNDLQGSCRS"
     CDS             complement(2475..2486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 2474"
                     /translation="IEGR"
     misc_feature    2647..2668
                     /label=U3PPT
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    2647..2662
                     /label=cPPT
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     LTR             2664..2897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2664..2716
                     /label=delta_U3
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    2717..2897
                     /label=HIV-1_5_LTR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    2717..2897
                     /label=truncHIV-1_3_LTR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     polyA_signal    2975..3096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     terminator      2978..3097
                     /label=SV40_PA_terminator
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    3066..3085
                     /label=EBV_rev_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     rep_origin      3115..3213
                     /label=SV40_origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     rep_origin      3136..3271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     misc_feature    3198..3217
                     /label=SV40pro_F_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     promoter        complement(3292..3310)
                     /label=T7_promoter
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     primer_bind     complement(3320..3336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        complement(3320..3336)
                     /label=M13_forward20_primer promoter
                     /label=M13_forward20_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    3329..3477
                     /label=lacZ_a
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    complement(3329..3351)
                     /label=M13_pUC_fwd_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     rep_origin      3478..3933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     rep_origin      3495..3801
                     /label=f1_origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     promoter        3959..4063
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3994..4022
                     /label=AmpR_promoter
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     gene            4064..4924
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             4064..4924
                     /codon_start=1
                     /label=ORF frame 2(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=ORF frame 2
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5079..5698
                     /label=pBR322_origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     rep_origin      5095..5683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    5971..5992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        6007..6037
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 6007 .. 6012 / #ffffff / -35
                        2: 6013 .. 6030 / #ffffff
                        3: 6031 .. 6037 / #ffffff / -10"
     promoter        6007..6036
                     /label=lac_promoter
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     protein_bind    6045..6061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     misc_feature    6050..6072
                     /label=M13_pUC_rev_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     primer_bind     6069..6085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        6071..6089
                     /label=M13_reverse_primer promoter
                     /label=M13_reverse_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     promoter        6106..6125
                     /label=T3_promoter
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     promoter        6106..6124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6152..6378
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             6379..6559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    6379..6559
                     /label=HIV-1_5_LTR(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=HIV-1_5_LTR
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    6379..6559
                     /label=truncHIV-1_3_LTR(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=truncHIV-1_3_LTR
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    6606..6731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    6670..6714
                     /label=HIV-1_psi_pack
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     CDS             7102..7977
                     /codon_start=1
                     /label=ORF frame 1(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=ORF frame 1
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALF
                     LGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQA
                     RILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINN
                     YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLR
                     IVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPREGLFPMIPSYLHIRYKAVREIIRINLT
                     VNTKILVQNT"
     misc_feature    7224..7457
                     /label=RRE
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     promoter        7864..8104
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        7873..8105
                     /label=hU6_promoter
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    8035..8054
                     /label=LKO1_5_primer
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
     misc_RNA        9994..10069
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     misc_feature    10125..10242
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    10129..10144
                     /label=cPPT(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=cPPT
                     ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb"
ORIGIN
        1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
      421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
      481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
      541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
      601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
      661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
      721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
      781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
      841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
      901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
      961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     1261 cgtgacgtac ggccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     1321 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     1381 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     1441 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     1501 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     1561 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     1621 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     1681 cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc tccgagcgga
     1741 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     1801 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     1861 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     1921 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     1981 acaagtaaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     2041 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2101 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2161 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2221 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2281 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2341 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     2401 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     2461 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     2521 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     2581 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     2641 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     2701 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     2761 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     2821 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     2881 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     2941 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     3001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     3121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3241 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     3301 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     3481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3901 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     3961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4021 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4081 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4141 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4201 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4261 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4321 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4381 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4441 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4501 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4561 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4621 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4921 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5221 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     5881 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     5941 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6001 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6061 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     6121 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     6181 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     6241 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     6301 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     6361 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6421 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6481 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6541 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     6601 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     6661 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     6721 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     6781 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     6841 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     6901 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     6961 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     7021 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     7081 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     7141 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7201 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7261 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     7321 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     7381 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     7441 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     7501 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     7561 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     7621 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     7681 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     7741 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     7801 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     7861 agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     7921 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     7981 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     8041 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     8101 ggacgaaaca ccggagacgg ttgtaaatga gcacacaaaa tacacatgct aaaatattat
     8161 attctatgac ctttataaaa tcaaccaaaa tcttcttttt aataacttta gtatcaataa
     8221 ttagaatttt tatgttcctt tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg
     8281 ctagttttag taactcgcgt tgttttcttc acctttaata atagctactc caccacttgt
     8341 tcctaagcgg tcagctcctg cttcaatcat tttttgagca tcttcaaatg ttctaactcc
     8401 accagctgct ttaactaaag cattgtcttt aacaactgac ttcattagtt taacatcttc
     8461 aaatgttgca cctgattttg aaaatcctgt tgatgtttta acaaattcta atccagcttc
     8521 aacagctatt tcacaagctt tcatgatttc ttcttttgtt aataaacaat tttccataat
     8581 acatttaaca acatgtgatc cagctgcttt ttttacagct ttcatgtctt ctaaaactaa
     8641 ttcataattt ttgtctttta atgcaccaat atttaatacc atatcaattt ctgttgcacc
     8701 atctttaatt gcttcagaaa cttcgaatgc ttttgtagct gttgtgcatg cacctagagg
     8761 aaaacctaca acatttgtta ttcctacatt tgtgcctttt aataattctt tacaatagct
     8821 tgttcaatat gaattaacac aaactgttgc aaaatcaaat tcaattgctt catcacataa
     8881 ttgtttaatt tcagctttcg tagcatcttg ttttaataat gtgtgatcta tatatttgtt
     8941 tagtttcatt ttttctccta tatattcatt tttaatttta attctttaat aatttcgtct
     9001 actttaactt tagcgttttg aacagattca ccaacaccta taaaataaat ttttagttta
     9061 ggttcagttc cacttgggcg aacagcaaat catgacttat cttctaaata aaattttagt
     9121 aagtcttgtc ctggcatatt atacattcca tcgatgtagt cttcaacatt aacaacttta
     9181 agtccagcaa tttgagttaa gggtgttgct ctcaatgatt tcattaatgg ttcaattttt
     9241 aatttctttt cttctggttt aaaattcaag tttaaagtga aagtgtaata tgcacccatt
     9301 tctttaaata aatcttctaa atagtctact aatgttttat tttgtttttt ataaaatcaa
     9361 gcagcctctg ctattaatat agaagcttgt attccatctt tatctctagc tgagtcatca
     9421 attacatatc cataactttc ttcataagca aaaacaaaat ttaatccgtt atcttcttct
     9481 ttagcaattt ctctacccat tcatttaaat ccagttaaag tttttacaat attaactcca
     9541 tatttttcat gagcgattct atcacccaaa tcacttgtta caaaacttga atatagagcc
     9601 ggattttttg gaatgctatt taagcgtttt agatttgata attttcaatc aattaaaatt
     9661 ggtcctgttt gatttccatc taatcttaca aaatgaccat catgttttat tgccattcca
     9721 aatctgtcag catctgggtc attcataata ataatatctg catcatgttt aataccatat
     9781 tcaagcggta tttttcatgc aggatcaaat tctggatttg gatttacaac atttttaaat
     9841 gtttcatctt caaatgcatg ctcttcaacc tcaataacgt tatatcctga ttcacgtaat
     9901 atttttgggg taaatttagt tcctgttcca ttaactgcgc taaaaataat ttttaaatct
     9961 tttttagctt cttgctcttt tttgtacgtc tctgttttag agctagaaat agcaagttaa
    10021 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct
    10081 tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
    10141 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
    10201 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
    10261 agcagagatc cactttggcg ccggctcgag gggg
//
