LOCUS       Exported                7870 bp ds-DNA     circular SYN 21-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2017 from SnapGene Viewer 4.1.0
            http://www.snapgene.com
COMMENT     Ligation of : ideal hTtAgo.xdna [5758 nt] : (NheI[2818] / FseI[709])
            to 15ABJBSC_1695841_TtAgo.gb.xdna [2104 nt] : (FseI[400] / 
            NheI[2509])
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    196..405
                     /label=CMV promoter
     misc_feature    529..552
                     /label=SV40 NLS1
     misc_feature    568..633
                     /label=3XFLAG
     CDS             610..633
                     /label=Flag tag
     misc_feature    715..2818
                     /label=hTtAgo
                     /note="note=""Fragment extracted from 
                     15ABJBSC_1695841_TtAgo.gb.xdna [2104 nt] : (FseI[400] / 
                     NheI[2509])"
     misc_feature    721..2817
                     /label=hTtAgo
     misc_feature    2848..2895
                     /label=Nucleoplasmin NLS
                     /note="note=""direct strand 
                     translation""translation=""KRPAATKKAGQAKKKK"""
     misc_feature    2848..2895
                     /label=Nucleoplasmin NLS
     misc_feature    2914..2970
                     /label=P2A(PG/P)
     misc_feature    2914..2970
                     /label=P2A
     misc_feature    3771..3986
                     /label=SV40 enhancer-pBabe
     misc_feature    3950..4027
                     /label=SV40 ORI
     CDS             4169..4960
                     /label=Kan/neoR
     primer_bind     complement(5302..5322)
                     /label=M13-rev
     misc_binding    complement(5328..5350)
                     /label=LacO
     promoter        complement(5355..5384)
                     /label=lac
     rep_origin      complement(5690..6318)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6470..7129)
                     /label=AmpR
     promoter        complement(7369..7397)
                     /label=Amp prom
     promoter        7774..430
                     /label=CMV promoter
     misc_feature    7778..195
                     /label=CAG enhancer
ORIGIN
        1 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
       61 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      121 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      181 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      241 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      301 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      361 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      481 gaccgatcca gcctccggac tctagaggat cgaacccttg ccaccatgcc caagaagaag
      541 aggaaggtgg gaggctccgg aggaagcgac tataaggacc acgacggaga ctacaaggat
      601 catgatattg attacaaaga cgatgacgat aagggtggcg ggtccggcgg tggatccggt
      661 accggaggga gtggtggaag cggcggttct ggtggctcag gcggcagcgg ccggccaatg
      721 aatcacctgg gaaagaccga ggtgttcctg aacagattcg ccctgcggcc cctgaacccc
      781 gaggaactga ggccttggag actggaagtg gtgctggacc ctccacccgg cagagaagag
      841 gtgtaccctc tgctggccca ggtggcaaga cgggctggcg gagtgacagt gcggatggga
      901 gatggactgg ccagctggtc cccacctgaa gtgctggtgc tggaaggcac cctggccaga
      961 atgggccaga cctacgccta ccggctgtac cccaagggca gaaggcccct ggaccctaag
     1021 gatcccggcg agagatctgt gctgagcgcc ctggctagac ggctgctgca ggaaagactg
     1081 cggcgcctgg aaggcgtgtg ggtggaagga ctggccgtgt acagaagaga gcacgccaga
     1141 ggacctggct ggcgggtgct gggcggagct gtgctggatc tgtgggtgtc agatagcggc
     1201 gccttcctgc tggaagtgga ccccgcctac agaatcctgt gcgagatgag cctggaagct
     1261 tggctggctc agggccaccc cctgcctaaa agagtgcgga acgcctacga ccggcggacc
     1321 tgggaactgc tgagactggg cgaagaggac cccaaagagc tgcctctgcc tggcggactg
     1381 agcctgctgg attaccacgc ctctaagggc cggctgcagg gaagagaagg cggcagagtg
     1441 gcttgggtgg ccgatcccaa ggaccccaga aagcccatcc ctcacctgac cggactgctg
     1501 gtgcctgtgc tgaccctgga agatctgcac gaggaagagg gcagcctggc cctgtctctg
     1561 ccctgggagg aaagacgcag acggaccaga gagatcgcca gctggatcgg cagacggctg
     1621 ggactgggaa cacctgaagc tgtgcgggcc caggcctaca gactgagcat ccctaagctg
     1681 atggggcgga gagccgtgtc caaacctgcc gatgctctga gagtgggctt ctacagagcc
     1741 caggaaaccg ctctggccct gctgaggctg gatggcgctc agggatggcc cgagtttctg
     1801 agaagggctc tgctgagagc cttcggcgcc tctggcgctt ctctgagact gcacacactg
     1861 cacgcccacc ctagtcaggg cctggccttt agagaggccc tgcggaaggc caaagaagag
     1921 ggggtgcagg ctgtgctggt gctgacacct cctatggcct gggaagatcg gaaccggctg
     1981 aaagccctgc tgctgaggga aggcctgccc agccagatcc tgaacgtgcc cctgcgggaa
     2041 gaggaacggc acagatggga gaatgccctg ctgggcctgc tggccaaagc tggactgcag
     2101 gtggtggctc tgtctggcgc ctatccagct gagctggccg tgggctttga tgccggcgga
     2161 agagagagct tcagattcgg cggagccgcc tgtgcagtgg gcggagatgg cggacatctg
     2221 ctgtggacac tgcctgaagc ccaggccggc gaaagaatcc cccaggaagt cgtgtgggat
     2281 ctgctggaag agacactgtg ggccttcaga cggaaggccg gcagactgcc tagcagagtg
     2341 ctgctgctgc gcgacggaag agtgccccag gatgaattcg cactggccct ggaagccctg
     2401 gcccgggaag gcattgccta cgatctggtg tccgtgcgga agtccggcgg aggcagagtg
     2461 tatcctgtgc agggcagact ggctgacggc ctgtatgtgc ctctggaaga taagaccttt
     2521 ctgctgctga ccgtgcaccg ggacttcaga ggcaccccca gacctctgaa gctggtgcac
     2581 gaagccggcg acacaccact ggaagctctg gcacaccaga ttttccacct gacccgcctg
     2641 taccccgcca gcggctttgc ctttcctaga ctgcctgccc ctctgcacct ggccgaccgg
     2701 ctcgtgaaag aagtgggcag gctgggcatc cggcacctga aagaggtgga ccgcgagaag
     2761 ctgttcttcg tgggaggctc tggcggatcc gggggatctg gcggaagcgg aggatctgct
     2821 agcggtggag gctccggagg tggatctaaa aggccggcgg ccacgaaaaa ggccggtcag
     2881 gcaaaaaaga aaaagggtgg tagtggaagc ggagctacta acttcagcct gctgaagcag
     2941 gctggagacg tggaggagaa ccctggacct gcggccgcat gaaagggttc gatccctacc
     3001 ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga
     3061 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     3121 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     3181 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     3241 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc
     3301 gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3361 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3421 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3481 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3541 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3601 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3661 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa
     3721 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     3781 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3841 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3901 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3961 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4021 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4081 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4141 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4201 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4261 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4321 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4381 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4441 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4501 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4561 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4621 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4681 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4741 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4801 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4861 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4921 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg
     4981 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     5041 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5101 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     5161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     5281 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5341 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5401 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5461 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5521 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5581 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5641 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5701 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5761 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5821 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5881 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     5941 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6001 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6061 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6121 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     6181 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6241 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6301 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6361 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6421 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6481 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6541 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6601 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6661 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6721 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6781 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6841 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6901 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6961 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7021 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7081 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7141 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7201 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7261 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7321 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7441 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac
     7501 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     7561 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     7621 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     7681 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     7741 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     7801 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     7861 agtaacgcca
//
