LOCUS       PB510B_1_updated_0        6454 bp ds-DNA   circular     01-OCT-2012
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
            
            
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(3866..4723)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=Amp
                     /ApEinfo_fwdcolor="#7ab3ee"
                     /ApEinfo_revcolor="#c3c2c4"
     primer_bind     complement(2730..2749)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=pLac(F)
                     /ApEinfo_fwdcolor="#5381ff"
                     /ApEinfo_revcolor="#19ff25"
     primer_bind     812..831
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=BII(R)
                     /ApEinfo_fwdcolor="#5381ff"
                     /ApEinfo_revcolor="#19ff25"
     repeat_unit     complement(join(874..886,918..919,921..936))
                     /dnas_title="5'TR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /dnas_title="arrow_data {{0 1 2 0 0 -1} {} 0} width 5
                     offset 0%1E%1F%1F"
     enhancer        1112..1343
                     /dnas_title="core insul"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#d7cccc"
                     /ApEinfo_revcolor="green"
     enhancer        complement(1672..1902)
                     /dnas_title="core insul"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=core insul(1)
                     /ApEinfo_label="core insul"
                     /ApEinfo_fwdcolor="#d8cac8"
                     /ApEinfo_revcolor="#d8c8c8"
     repeat_unit     2177..2211
                     /dnas_title="3'TR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=3'TR
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     repeat_unit     complement(887..917)
                     /dnas_title="5'TR(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_label="5'TR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    887..917
                     /dnas_title="31bp spacer"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#43ffb8"
                     /ApEinfo_revcolor="#ff77e0"
     PCR_primer      complement(1342..1360)
                     /label=PB-CMV-EF1-Puro_n1360_R
                     /note="Ordered sequence:            
                     PB-CMV-EF1-Puro_n1366_R: AGGGAGTAAACTCGAGGAATTCTTG
                     tagtGGCCgTGGAGGCCTT"
                     /note=""
     primer_bind     841..860
                     /label=PB-CMV-EF1-Puro_n841_F
     primer_bind     1339..1358
                     /label=PB-Cas9-Hyg_n1340_F
     PCR_primer      complement(3893..3913)
                     /note="PB-CMV-EF1-Puro_n5644_R"
     PCR_primer      3887..3906
                     /note="PB-CMV-EF1-Puro_n5618_F"
     PCR_primer      1543..1565
                     /note="Sp-Hyg-LV_F1"
     promoter        1375..1616
                     /note="U6 Promoter"
     primer_bind     1606..1626
                     /note="151215_LibPrimF"
     primer_bind     1374..1392
                     /note="160601_U6-F"
     primer_bind     complement(1613..1630)
                     /note="160601_U6-R"
     primer_bind     complement(2257..2276)
                     /note="PB-CMV-EF1-Puro_n4007_R"
     source          1..4723
                     /dnas_title="PB510B-1_100112_map_from_SystemBiosciences"
ORIGIN      
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
      181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
      241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
      301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
      361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
      421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
      481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
      541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
      601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
      661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
      721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG
      781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG
      841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG
      901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG
      961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA
     1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA
     1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG
     1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC
     1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG
     1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA
     1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCacta gtctagagct agccgagggc
     1381 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     1441 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     1501 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     1561 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     1621 accGGTCCCC GGATCCGATT TAAATTCGAA TTCGGGCCCA TTGGTATGGC TTTTTCCCCG
     1681 TATCCCCCCA GGTGTCTGCA GGCTCAAAGA GCAGCGAGAA GCGTTCAGAG GAAAGCGATC
     1741 CCGTGCCACC TTCCCCGTGC CCGGGCTGTC CCCGCACGCT GCCGGCTCGG GGATGCGGGG
     1801 GGAGCGCCGG ACCGGAGCGG AGCCCCGGGC GGCTCGCTGC TGCCCCCTAG CGGGGGAGGG
     1861 ACGTAATTAC ATCCCTGGGG GCTTTGGGGG GGGGCTGTCC CTGATATCTA TAACAAGAAA
     1921 ATATATATAT AATAAGTTAT CACGTAAGTA GAACATGAAA TAACAATATA ATTATCGTAT
     1981 GAGTTAAATC TTAAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG
     2041 TTATAGTTCA AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA
     2101 GCGGCGACTG AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG
     2161 CAATATTTCA AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAATCTAG
     2221 CTGCATCAGG ATCATATCGT CGGGTCTTTT TTCCGGCTCA GTCATCGCCC AAGCTGGCGC
     2281 TATCTGGGCA TCGGGGAGGA AGAAGCCCGT GCCTTTTCCC GCGAGGTTGA AGCGGCATGG
     2341 AAAGAGTTTG CCGAGGATGA CTGCTGCTGC ATTGACGTTG AGCGAAAACG CACGTTTACC
     2401 ATGATGATTC GGGAAGGTGT GGCCATGCAC GCCTTTAACG GTGAACTGTT CGTTCAGGCC
     2461 ACCTGGGATA CCAGTTCGTC GCGGCTTTTC CGGACACAGT TCCGGATGGT CAGCCCGAAG
     2521 CGCATCAGCA ACCCGAACAA TACCGGCGAC AGCCGGAACT GCCGTGCCGG TGTGCAGATT
     2581 AATGACAGCG GTGCGGCGCT GGGATATTAC GTCAGCGAGG ACGGGTATCC TGGCTGGATG
     2641 CCGCAGAAAT GGACATGGAT ACCCCGTGAG TTACCCGGCG GGCGCGCTTG GCGTAATCAT
     2701 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG
     2761 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG
     2821 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA
     2881 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA
     2941 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
     3001 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
     3061 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
     3121 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
     3181 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
     3241 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
     3301 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
     3361 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
     3421 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
     3481 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
     3541 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
     3601 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
     3661 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
     3721 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
     3781 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
     3841 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
     3901 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
     3961 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG
     4021 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
     4081 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
     4141 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT
     4201 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT
     4261 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA
     4321 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA
     4381 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT
     4441 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC
     4501 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA
     4561 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT
     4621 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG
     4681 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CAT
//

