LOCUS       PB510B_1_updated_0        6454 bp ds-DNA   circular     01-OCT-2012
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
            
            
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(5514..6371)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=Amp
                     /ApEinfo_fwdcolor="#7ab3ee"
                     /ApEinfo_revcolor="#c3c2c4"
     misc_feature    2826..2930
                     /dnas_title="WPRE"
                     /vntifkey="21"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=WPRE
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#0080ff"
     primer_bind     complement(4378..4397)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=pLac(F)
                     /ApEinfo_fwdcolor="#5381ff"
                     /ApEinfo_revcolor="#19ff25"
     primer_bind     812..831
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=BII(R)
                     /ApEinfo_fwdcolor="#5381ff"
                     /ApEinfo_revcolor="#19ff25"
     repeat_unit     complement(join(874..886,918..919,921..936))
                     /dnas_title="5'TR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /dnas_title="arrow_data {{0 1 2 0 0 -1} {} 0} width 5
                     offset 0%1E%1F%1F"
     enhancer        1112..1343
                     /dnas_title="core insul"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#d7cccc"
                     /ApEinfo_revcolor="green"
     enhancer        complement(3320..3550)
                     /dnas_title="core insul"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=core insul(1)
                     /ApEinfo_label="core insul"
                     /ApEinfo_fwdcolor="#d8cac8"
                     /ApEinfo_revcolor="#d8c8c8"
     repeat_unit     3825..3859
                     /dnas_title="3'TR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=3'TR
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    2220..2816
                     /dnas_title="puro"
                     /vntifkey="21"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=puro
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#0080ff"
     repeat_unit     complement(887..917)
                     /dnas_title="5'TR(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_label="5'TR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     promoter        1669..2214
                     /dnas_title="EF1 promoter"
                     /vntifkey="29"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=EF1 promoter
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_feature    887..917
                     /dnas_title="31bp spacer"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#43ffb8"
                     /ApEinfo_revcolor="#ff77e0"
     misc_feature    join(2936..2939,2944..2944,2946..3071)
                     /dnas_title="SV40 pA"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label=SV40 pA
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /dnas_title="arrow_data {{0 1 2 0 0 -1} {} 0} width 5
                     offset 0%1E%1F%1F"
     PCR_primer      complement(1668..1687)
                     /dnas_title="PB510B-MCS-R"
                     /primer="ggagcgatcgcagatccttc"
     PCR_primer      2818..2837
                     /label=PB-CMV-EF1-Puro_n2901_F
                     /note="Ordered sequence:      PB-CMV-EF1-Puro_n2901_F:
                     TACCCATACGATGTTCCAGATTACGCTTAG gagtcgacaatcaacctctg"
     PCR_primer      complement(1342..1360)
                     /label=PB-CMV-EF1-Puro_n1360_R
                     /note="Ordered sequence:            
                     PB-CMV-EF1-Puro_n1366_R: AGGGAGTAAACTCGAGGAATTCTTG
                     tagtGGCCgTGGAGGCCTT"
                     /note=""
     primer_bind     841..860
                     /label=PB-CMV-EF1-Puro_n841_F
     primer_bind     complement(3225..3244)
                     /label=PB-CMV-EF1-Puro_n3327_R
     primer_bind     1339..1358
                     /label=PB-Cas9-Hyg_n1340_F
     PCR_primer      complement(5541..5561)
                     /note="PB-CMV-EF1-Puro_n5644_R"
     PCR_primer      5535..5554
                     /note="PB-CMV-EF1-Puro_n5618_F"
     promoter        1375..1616
                     /note="U6 Promoter"
     primer_bind     1374..1392
                     /note="160601_U6-F"
     PCR_primer      1543..1565
                     /note="Sp-Hyg-LV_F1"
     primer_bind     1606..1626
                     /note="151215_LibPrimF"
     primer_bind     complement(1613..1630)
                     /note="160601_U6-R"
     primer_bind     complement(3905..3924)
                     /note="PB-CMV-EF1-Puro_n4007_R"
     source          1..6371
                     /dnas_title="PB510B-1_100112_map_from_SystemBiosciences"
ORIGIN      
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
      181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
      241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
      301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
      361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
      421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
      481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
      541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
      601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
      661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
      721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG
      781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG
      841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG
      901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG
      961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA
     1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA
     1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG
     1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC
     1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG
     1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA
     1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCacta gtctagagct agccgagggc
     1381 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     1441 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     1501 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     1561 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     1621 accGGTCCCC GGATCCGATT TAAATTCGAA TTCGGGCCCg cggccgcgaa ggatctgcga
     1681 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     1741 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     1801 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     1861 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     1921 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     1981 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     2041 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     2101 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     2161 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     2221 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     2281 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     2341 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     2401 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     2461 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     2521 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     2581 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     2641 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     2701 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     2761 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     2821 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2881 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat GcgTTAACTa
     2941 aacTtGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA
     3001 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT
     3061 TATCATGTCT GGAATTGACT CAAATGATGT CAATTAGTCT ATCAGAAGCT cATCTGGTCT
     3121 CCCTTCCGGG GGACAAGACA TCCCTGTTTA ATATTTAAAC AGCAGTGTTC CCAAACTGGG
     3181 TTCTTATATC CCTTGCTCTG GTCAACCAGG TTGCAGGGTT TCCTGTCCTC ACAGGAACGA
     3241 AGTCCCTAAA GAAACAGTGG CAGCCAGGTT TAGCCCCGGA ATTGACTGGA TTCCTTTTTT
     3301 AGGGCCCATT GGTATGGCTT TTTCCCCGTA TCCCCCCAGG TGTCTGCAGG CTCAAAGAGC
     3361 AGCGAGAAGC GTTCAGAGGA AAGCGATCCC GTGCCACCTT CCCCGTGCCC GGGCTGTCCC
     3421 CGCACGCTGC CGGCTCGGGG ATGCGGGGGG AGCGCCGGAC CGGAGCGGAG CCCCGGGCGG
     3481 CTCGCTGCTG CCCCCTAGCG GGGGAGGGAC GTAATTACAT CCCTGGGGGC TTTGGGGGGG
     3541 GGCTGTCCCT GATATCTATA ACAAGAAAAT ATATATATAA TAAGTTATCA CGTAAGTAGA
     3601 ACATGAAATA ACAATATAAT TATCGTATGA GTTAAATCTT AAAAGTCACG TAAAAGATAA
     3661 TCATGCGTCA TTTTGACTCA CGCGGTCGTT ATAGTTCAAA ATCAGTGACA CTTACCGCAT
     3721 TGACAAGCAC GCCTCACGGG AGCTCCAAGC GGCGACTGAG ATGTCCTAAA TGCACAGCGA
     3781 CGGATTCGCG CTATTTAGAA AGAGAGAGCA ATATTTCAAG AATGCATGCG TCAATTTTAC
     3841 GCAGACTATC TTTCTAGGGT TAATCTAGCT GCATCAGGAT CATATCGTCG GGTCTTTTTT
     3901 CCGGCTCAGT CATCGCCCAA GCTGGCGCTA TCTGGGCATC GGGGAGGAAG AAGCCCGTGC
     3961 CTTTTCCCGC GAGGTTGAAG CGGCATGGAA AGAGTTTGCC GAGGATGACT GCTGCTGCAT
     4021 TGACGTTGAG CGAAAACGCA CGTTTACCAT GATGATTCGG GAAGGTGTGG CCATGCACGC
     4081 CTTTAACGGT GAACTGTTCG TTCAGGCCAC CTGGGATACC AGTTCGTCGC GGCTTTTCCG
     4141 GACACAGTTC CGGATGGTCA GCCCGAAGCG CATCAGCAAC CCGAACAATA CCGGCGACAG
     4201 CCGGAACTGC CGTGCCGGTG TGCAGATTAA TGACAGCGGT GCGGCGCTGG GATATTACGT
     4261 CAGCGAGGAC GGGTATCCTG GCTGGATGCC GCAGAAATGG ACATGGATAC CCCGTGAGTT
     4321 ACCCGGCGGG CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
     4381 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
     4441 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
     4501 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
     4561 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
     4621 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
     4681 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     4741 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     4801 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     4861 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     4921 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     4981 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     5041 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     5101 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     5161 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG
     5221 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     5281 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     5341 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     5401 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     5461 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     5521 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     5581 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     5641 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     5701 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     5761 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     5821 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     5881 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     5941 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     6001 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     6061 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     6121 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     6181 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     6241 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     6301 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     6361 TGAATACTCA T
//

