LOCUS       3G-hMYCN    6579 bp      DNA     circular   11/8/17
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=GateWay(tm) LR reaction between human MYCN Entry Vector as a
            n Entry vector and PB-TRE3G Destination corrected  as a Dest
            ination vector.
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    3607..3948
                     /label=AHS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=/vntifkey="21" - /label=AHS - 6785..7126 \ 
     misc_feature    838..1411
                     /label=CHS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=/vntifkey="21" - /label=CHS - 830..1403 \ 
     rep_origin      complement(4744..5372)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5524..6183)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     polyA_signal    3348..3539
                     /label=SV40 late polyA
                     /SerialCloner_Color=&hFFC0CB
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6423..6451)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    416..651
                     /label=3 TR PB
                     /SerialCloner_Color=&hFF0A28
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4032..4336
                     /label=5 TR PB
                     /SerialCloner_Color=&hFF0E21
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1433..1808
                     /label=TRE3G
                     /SerialCloner_Color=&h800000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1868..3262
                     /label=MYCN
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 
       61 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 
      121 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 
      181 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 
      241 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 
      301 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 
      361 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCaagcttT ATTATTTAAC 
      421 CCTAGAAAGA TAATCATATT GTGACGTACG TTAAAGATAA TCATGCGTAA AATTGACGCA 
      481 TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT ATTTATTAAT TTGAATAGAT ATTAAGTTTT 
      541 ATTATATTTA CACTTACATA CTAATAATAA ATTCAACAAA CAATTTATTT ATGTTTATTT 
      601 ATTTATTAAA AAAAAACAAA AACTCAAAAT TTCTTCTATA AAGTAACAAA ACTTTTAAAC 
      661 ATTCTCTCTT TTACAAAAAT AAACTTATTT TGTACTTTAA AAACAGTCAT GTTGTAATCG 
      721 ATAATAAGTA ATTAGCTTAA CCTATACATA ATAGAAACAA ATTATACTTA TTAGTCAGTC 
      781 AGAAACAACT TTGGCACATA TCAATATTAT GCTCTCGTTA ATTAACTCGA GGGATCCGGC 
      841 GCGCCTGTCA TTCTAAATCT CTCTTTCAGC CTAAAGCTTT TTCCCCGTAT CCCCCCAGGT 
      901 GTCTGCAGGC TCAAAGAGCA GCGAGAAGCG TTCAGAGGAA AGCGATCCCG TGCCACCTTC 
      961 CCCGTGCCCG GGCTGTCCCC GCACGCTGCC GGCTCGGGGA TGCGGGGGGA GCGCCGGACC 
     1021 GGAGCGGAGC CCCGGGCGGC TCGCTGCTGC CCCCTAGCGG GGGAGGGACG TAATTACATC 
     1081 CCTGGGGGCT TTGGGAGGGG GCTGTCCCCG TGAGCTCCCA GGCGCGCCTG TCATTCTAAA 
     1141 TCTCTCTTTC AGCCTAAAGC TTTTTCCCCG TATCCCCCCA GGTGTCTGCA GGCTCAAAGA 
     1201 GCAGCGAGAA GCGTTCAGAG GAAAGCGATC CCGTGCCACC TTCCCCGTGC CCGGGCTGTC 
     1261 CCCGCACGCT GCCGGCTCGG GGATGCGGGG GGAGCGCCGG ACCGGAGCGG AGCCCCGGGC 
     1321 GGCTCGCTGC TGCCCCCTAG CGGGGGAGGG ACGTAATTAC ATCCCTGGGG GCTTTGGGGG 
     1381 GGGGCTGTCC CCGTGAGCTC CCAGGCGCGC CGctagCCAA GAATTCCTCG AGTTTACTCC 
     1441 CTATCAGTGA TAGAGAACGT ATGAAGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGC 
     1501 AGACTTTACT CCCTATCAGT GATAGAGAAC GTATAAGGAG TTTACTCCCT ATCAGTGATA 
     1561 GAGAACGTAT GACCAGTTTA CTCCCTATCA GTGATAGAGA ACGTATCTAC AGTTTACTCC 
     1621 CTATCAGTGA TAGAGAACGT ATATCCAGTT TACTCCCTAT CAGTGATAGA GAACGTATAA 
     1681 GCTTTAGGCG TGTACGGTGG GCGCCTATAA AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT 
     1741 CGCCTGGAGC AATTCCACAA CACTTTTGTC TTATACCAAC TTTCCGTACC ACTTCCTACC 
     1801 CTCGTAAACC gcGGTGGCGG CCATCACAAG TTTGTACAAA AAAGCAGGCT Taaggagttt 
     1861 aaacaccatg CCGAGCTGCT CCACGTCCAC CATGCCGGGC ATGATCTGCA AGAACCCAGA 
     1921 CCTCGAGTTT GACTCGCTAC AGCCCTGCTT CTACCCGGAC GAAGATGACT TCTACTTCGG 
     1981 CGGCCCCGAC TCGACCCCCC CGGGGGAGGA CATCTGGAAG AAGTTTGAGC TGCTGCCCAC 
     2041 GCCCCCGCTG TCGCCCAGCC GTGGCTTCGC GGAGCACAGC TCCGAGCCCC CGAGCTGGGT 
     2101 CACGGAGATG CTGCTTGAGA ACGAGCTGTG GGGCAGCCCG GCCGAGGAGG ACGCGTTCGG 
     2161 CCTGGGGGGA CTGGGTGGCC TCACCCCCAA CCCGGTCATC CTCCAGGACT GCATGTGGAG 
     2221 CGGCTTCTCC GCCCGCGAGA AGCTGGAGCG CGCCGTGAGC GAGAAGCTGC AGCACGGCCG 
     2281 CGGGCCGCCA ACCGCCGGTT CCACCGCCCA GTCCCCGGGA GCCGGCGCCG CCAGCCCTGC 
     2341 GGGTCGCGGG CACGGCGGGG CTGCGGGAGC CGGCCGCGCC GGGGCCGCCC TGCCCGCCGA 
     2401 GCTCGCCCAC CCGGCCGCCG AGTGCGTGGA TCCCGCCGTG GTCTTCCCCT TTCCCGTGAA 
     2461 CAAGCGCGAG CCAGCGCCCG TGCCCGCAGC CCCGGCCAGT GCCCCGGCGG CGGGCCCTGC 
     2521 GGTCGCCTCG GGGGCGGGTA TTGCCGCCCC AGCCGGGGCC CCGGGGGTCG CCCCTCCGCG 
     2581 CCCAGGCGGC CGCCAGACCA GCGGCGGCGA CCACAAGGCC CTCAGTACCT CCGGAGAGGA 
     2641 CACCCTGAGC GATTCAGATG ATGAAGATGA TGAAGAGGAA GATGAAGAGG AAGAAATCGA 
     2701 CGTGGTCACT GTGGAGAAGC GGCGTTCCTC CTCCAACACC AAGGCTGTCA CCACATTCAC 
     2761 CATCACTGTG CGTCCCAAGA ACGCAGCCCT GGGTCCCGGG AGGGCTCAGT CCAGCGAGCT 
     2821 GATCCTCAAA CGATGCCTTC CCATCCACCA GCAGCACAAC TATGCCGCCC CCTCTCCCTA 
     2881 CGTGGAGAGT GAGGATGCAC CCCCACAGAA GAAGATAAAG AGCGAGGCGT CCCCACGTCC 
     2941 GCTCAAGAGT GTCATCCCCC CAAAGGCTAA GAGCTTGAGC CCCCGAAACT CTGACTCGGA 
     3001 GGACAGTGAG CGTCGCAGAA ACCACAACAT CCTGGAGCGC CAGCGCCGCA ACGACCTTCG 
     3061 GTCCAGCTTT CTCACGCTCA GGGACCACGT GCCGGAGTTG GTAAAGAATG AGAAGGCCGC 
     3121 CAAGGTGGTC ATTTTGAAAA AGGCCACTGA GTATGTCCAC TCCCTCCAGG CCGAGGAGCA 
     3181 CCAGCTTTTG CTGGAAAAGG AAAAATTGCA GGCAAGACAG CAGCAGTTGC TAAAGAAAAT 
     3241 TGAACACGCT CGGACTTGCt agaagagccc gggcgatcgc gaACCCAGCT TTCTTGTACA 
     3301 AAGTGGTGAT CCAACCGGTA TAGATCTCGA GCTCGATGAG TTTAAACCAG ACATGATAAG 
     3361 ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG 
     3421 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA 
     3481 CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA 
     3541 AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCCGGCTG CCTCGCGCTT 
     3601 AATTAACCCG GGGGCCGCCT GGGAACTGCC GCTGGCCCCC CACCGCCCCA AGGATCTCCG 
     3661 GTCCCCGCCC GGCTTGCTGA CGTCACGGAT GCCCCAGGGT GTGCTGGGCA GGTAGCGGGA 
     3721 GCCTGGGAAA TGGAGTCCAT TAGCAGAAGT GGCCACTTCC AGGAGTCGCT GTGCCCCGAT 
     3781 GCACACTGGG AAGTCCGCAG CTCCGAGGCC CCCAGTGGAA ATCGCCAGAT GAGGGCCTCC 
     3841 TCCGGGGAAT GCTGGGAAAT GGAGTCTACA GGCCGGAGGG GTCCCCACGG CATACTAGCA 
     3901 AGTGTGTGTA GCACCGCGTA AAGGGGATGA ATAGCAGACT GCCCCGGGAT AACTTCGTAT 
     3961 AGGATACTTT ATACGAAGTT ATGAGCTCTG TGATAATCAC TAGTGAATTG ATACTAGTCG 
     4021 GTCACGATAT CTATAACAAG AAAATATATA TATAATAAGT TATCACGTAA GTAGAACATG 
     4081 AAATAACAAT ATAATTATCG TATGAGTTAA ATCTTAAAAG TCACGTAAAA GATAATCATG 
     4141 CGTCATTTTG ACTCACGCGG TCGTTATAGT TCAAAATCAG TGACACTTAC CGCATTGACA 
     4201 AGCACGCCTC ACGGGAGCTC CAAGCGGCGA CTGAGATGTC CTAAATGCAC AGCGACGGAT 
     4261 TCGCGCTATT TAGAAAGAGA GAGCAATATT TCAAGAATGC ATGCGTCAAT TTTACGCAGA 
     4321 CTATCTTTCT AGGGTTAAAA AAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 
     4381 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 
     4441 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 
     4501 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 
     4561 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 
     4621 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4681 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4741 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4801 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4861 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4921 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4981 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5041 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5101 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5161 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5221 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5281 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5341 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5401 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5461 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 
     5521 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 
     5581 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 
     5641 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 
     5701 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 
     5761 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 
     5821 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 
     5881 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 
     5941 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 
     6001 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 
     6061 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 
     6121 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 
     6181 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 
     6241 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 
     6301 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 
     6361 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 
     6421 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 
     6481 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC 
     6541 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCG
//