LOCUS       PB-Tet3G_corrected    6706 bp      DNA     circular   11/8/17
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pDV021 [4590  nt] : (HpaI[4841] / AgeI[1495]) 
            to pEF1a-Tet3G.gb.xdna [2098  nt] : (AgeI[283] / HpaI[2383])
            
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2713..3459
                     /label=Tet-On\3G
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Tet-On3G - 1495..2241 \ 
     rep_origin      complement(4869..5497)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5649..6308)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     polyA_signal    3473..3664
                     /label=SV40 late polyA
                     /SerialCloner_Color=&hFFC0CB
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6548..6576)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    414..649
                     /label=3 TR PB
                     /SerialCloner_Color=&hFF0A28
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4157..4461
                     /label=5 TR PB
                     /SerialCloner_Color=&hFF0E21
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3732..4073
                     /label=AHS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    836..1409
                     /label=CHS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1411..2605
                     /label=EF1a promoter
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CAAGCTTTAT TATTTAACCC 
      421 TAGAAAGATA ATCATATTGT GACGTACGTT AAAGATAATC ATGCGTAAAA TTGACGCATG 
      481 TGTTTTATCG GTCTGTATAT CGAGGTTTAT TTATTAATTT GAATAGATAT TAAGTTTTAT 
      541 TATATTTACA CTTACATACT AATAATAAAT TCAACAAACA ATTTATTTAT GTTTATTTAT 
      601 TTATTAAAAA AAAACAAAAA CTCAAAATTT CTTCTATAAA GTAACAAAAC TTTTAAACAT 
      661 TCTCTCTTTT ACAAAAATAA ACTTATTTTG TACTTTAAAA ACAGTCATGT TGTAATCGAT 
      721 AATAAGTAAT TAGCTTAACC TATACATAAT AGAAACAAAT TATACTTATT AGTCAGTCAG 
      781 AAACAACTTT GGCACATATC AATATTATGC TCTCGTTAAT TAACTCGAGG GATCCGGCGC 
      841 GCCTGTCATT CTAAATCTCT CTTTCAGCCT AAAGCTTTTT CCCCGTATCC CCCCAGGTGT 
      901 CTGCAGGCTC AAAGAGCAGC GAGAAGCGTT CAGAGGAAAG CGATCCCGTG CCACCTTCCC 
      961 CGTGCCCGGG CTGTCCCCGC ACGCTGCCGG CTCGGGGATG CGGGGGGAGC GCCGGACCGG 
     1021 AGCGGAGCCC CGGGCGGCTC GCTGCTGCCC CCTAGCGGGG GAGGGACGTA ATTACATCCC 
     1081 TGGGGGCTTT GGGAGGGGGC TGTCCCCGTG AGCTCCCAGG CGCGCCTGTC ATTCTAAATC 
     1141 TCTCTTTCAG CCTAAAGCTT TTTCCCCGTA TCCCCCCAGG TGTCTGCAGG CTCAAAGAGC 
     1201 AGCGAGAAGC GTTCAGAGGA AAGCGATCCC GTGCCACCTT CCCCGTGCCC GGGCTGTCCC 
     1261 CGCACGCTGC CGGCTCGGGG ATGCGGGGGG AGCGCCGGAC CGGAGCGGAG CCCCGGGCGG 
     1321 CTCGCTGCTG CCCCCTAGCG GGGGAGGGAC GTAATTACAT CCCTGGGGGC TTTGGGGGGG 
     1381 GGCTGTCCCC GTGAGCTCCC AGGCGCGCCG ctagcttcgt gaggctccgg tgcccgtcag 
     1441 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 
     1501 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 
     1561 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 
     1621 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg 
     1681 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct 
     1741 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc 
     1801 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg 
     1861 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct 
     1921 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt 
     1981 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg 
     2041 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag 
     2101 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc 
     2161 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga 
     2221 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg 
     2281 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc 
     2341 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt 
     2401 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac 
     2461 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt 
     2521 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt 
     2581 ttttcttcca tttcaggtgt cgtgagagct cgtttagtga accgtcagat cgcctggaga 
     2641 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccgcggc 
     2701 cccgaattca ccatgtctag actggacaag agcaaagtca taaactctgc tctggaatta 
     2761 ctcaatggag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt 
     2821 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca 
     2881 atcgagatgc tggacaggca tcatacccac tcctgccccc tggaaggcga gtcatggcaa 
     2941 gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg 
     3001 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag 
     3061 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc 
     3121 gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag 
     3181 gaaagagaga cacctaccac cgattctatg cccccacttc tgaaacaagc aattgagctg 
     3241 ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc 
     3301 ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac 
     3361 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac 
     3421 gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga tccagacatg 
     3481 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt 
     3541 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa 
     3601 gttAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT 
     3661 TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCC GGCTGCCTCG 
     3721 CGCTTAATTA ACCCGGGGGC CGCCTGGGAA CTGCCGCTGG CCCCCCACCG CCCCAAGGAT 
     3781 CTCCGGTCCC CGCCCGGCTT GCTGACGTCA CGGATGCCCC AGGGTGTGCT GGGCAGGTAG 
     3841 CGGGAGCCTG GGAAATGGAG TCCATTAGCA GAAGTGGCCA CTTCCAGGAG TCGCTGTGCC 
     3901 CCGATGCACA CTGGGAAGTC CGCAGCTCCG AGGCCCCCAG TGGAAATCGC CAGATGAGGG 
     3961 CCTCCTCCGG GGAATGCTGG GAAATGGAGT CTACAGGCCG GAGGGGTCCC CACGGCATAC 
     4021 TAGCAAGTGT GTGTAGCACC GCGTAAAGGG GATGAATAGC AGACTGCCCC GGGATAACTT 
     4081 CGTATAGGAT ACTTTATACG AAGTTATGAG CTCTGTGATA ATCACTAGTG AATTGATACT 
     4141 AGTCGGTCAC GATATCTATA ACAAGAAAAT ATATATATAA TAAGTTATCA CGTAAGTAGA 
     4201 ACATGAAATA ACAATATAAT TATCGTATGA GTTAAATCTT AAAAGTCACG TAAAAGATAA 
     4261 TCATGCGTCA TTTTGACTCA CGCGGTCGTT ATAGTTCAAA ATCAGTGACA CTTACCGCAT 
     4321 TGACAAGCAC GCCTCACGGG AGCTCCAAGC GGCGACTGAG ATGTCCTAAA TGCACAGCGA 
     4381 CGGATTCGCG CTATTTAGAA AGAGAGAGCA ATATTTCAAG AATGCATGCG TCAATTTTAC 
     4441 GCAGACTATC TTTCTAGGGT TAAAAAAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 
     4501 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 
     4561 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 
     4621 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 
     4681 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 
     4741 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 
     4801 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 
     4861 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 
     4921 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 
     4981 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 
     5041 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 
     5101 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 
     5161 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 
     5221 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 
     5281 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT 
     5341 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 
     5401 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 
     5461 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 
     5521 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 
     5581 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 
     5641 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 
     5701 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 
     5761 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 
     5821 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 
     5881 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 
     5941 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 
     6001 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 
     6061 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 
     6121 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 
     6181 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 
     6241 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 
     6301 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 
     6361 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 
     6421 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 
     6481 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6541 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6601 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC 
     6661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC
//