LOCUS       Exported                7291 bp ds-DNA     circular SYN 12-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pmCN1-optoPKAW196RF327A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Colin O'Banion
  TITLE     Direct Submission
  JOURNAL   Exported Mar 12, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=PCMV IE
                     /label=PCMV\IE
                     /note="/vntifkey=29"
     enhancer        59..465
                     /label=CMV enhancer region
                     /label=CMV\enhancer\region
                     /note="/vntifkey=9"
     TATA_signal     554..560
                     /label=TATA\box
                     /note="/vntifkey=41"
     misc_feature    583
                     /label=transcription start point
                     /label=transcription\start\point
                     /note="/vntifkey=21"
     misc_feature    591..624
                     /label=MCS
                     /note="/vntifkey=21"
     misc_feature    625..2118
                     /label=Cry2PHR
                     /note="/vntifkey=21"
     misc_feature    2143..2850
                     /label=mCherry
                     /note="/vntifkey=21"
     misc_feature    2851..2898
                     /label=16aa linker
                     /label=16aa\linker
                     /note="/vntifkey=21"
     misc_feature    2899..3965
                     /label=PKAcs
                     /note="/vntifkey=21"
     misc_feature    3115..3117
                     /label=K72
                     /note="/vntifkey=21"
     variation       3487..3489
                     /label=W196R
                     /label=nonstandard type: mutation
                     /note="/vntifkey=62"
     variation       3511..3513
                     /label=Y204
                     /label=nonstandard type: mutation
                     /note="/vntifkey=62"
     variation       3880..3882
                     /label=F327A
                     /label=nonstandard type: mutation
                     /note="/vntifkey=62"
     polyA_signal    4110..4115
                     /label=SV40 polyA signals
                     /label=SV40\polyA\signals
                     /note="/vntifkey=25"
     polyA_signal    4139..4144
                     /label=SV40 poly A signal
                     /label=SV40\poly\A\signal
                     /note="/vntifkey=25"
     misc_feature    complement(4207..4642)
                     /label=f1 origin of replication
                     /label=f1\origin\of\replication
                     /note="/vntifkey=21"
     promoter        4719..4752
                     /label=PKanR
                     /note="/vntifkey=30"
     -35_signal      4724..4729
                     /label=-35 region PKanR
                     /label=-35\region\PKanR
                     /note="/vntifkey=48"
     -10_signal      4747..4752
                     /label=-10 region PKanR
                     /label=-10\region\PKanR
                     /note="/vntifkey=47"
     promoter        4836..5002
                     /label=PSV40 E
                     /label=PSV40\E
                     /note="/vntifkey=29"
     misc_feature    5003..5138
                     /label=SV40 ori
                     /label=SV40\ori
                     /note="/vntifkey=21"
     misc_feature    5187..5981
                     /label=KanR/NeoR
                     /note="/vntifkey=21"
     polyA_signal    6217..6222
                     /label=HSVTK poly A
                     /label=HSVTK\poly\A
                     /note="/vntifkey=25"
     polyA_signal    6230..6235
                     /label=HSVTK polyA
                     /label=HSVTK\polyA
                     /note="/vntifkey=25"
     misc_feature    6566..7209
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=21"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgaag atggacaaaa agactatagt ttggtttaga
      661 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt
      721 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca
      781 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct
      841 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt
      901 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac
      961 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat
     1021 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat
     1081 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg
     1141 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta
     1201 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca
     1261 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat
     1321 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat
     1381 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg
     1441 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt
     1501 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg
     1561 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga
     1621 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga
     1681 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt
     1741 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt
     1801 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc
     1861 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa
     1921 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct
     1981 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta
     2041 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag
     2101 atcatgatcg gagcagcagc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     2161 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2221 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2281 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2341 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     2401 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2461 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     2521 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     2581 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     2641 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     2701 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     2761 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     2821 tccaccggcg gcatggacga gctgtacaag agtgctggtg gtagtgctgg tggtagtgct
     2881 ggtggtagtg ctggtggtat gggcaacgcc gccgccgcca agaagggcag cgagcaggag
     2941 agcgtgaaag agttcctagc caaagccaag gaagatttcc tgaaaaaatg ggagacccct
     3001 tctcagaata cagcccagtt ggatcagttt gatagaatca agacccttgg caccggctcc
     3061 tttgggcgag tgatgctggt gaagcacaag gagagtggga accactacgc catgaagatc
     3121 ttagacaagc agaaggtggt gaagctaaag cagatcgagc acactctgaa tgagaagcgc
     3181 atcctgcagg ccgtcaactt cccgttcctg gtcaaacttg aattctcctt caaggacaac
     3241 tcaaacctgt atatggtcat ggagtatgta gctggtggcg agatgttctc ccacctacgg
     3301 cggattggaa ggttcagcga gccccatgcc cgtttctacg cggcgcagat cgtcctgacc
     3361 tttgagtatc tgcactccct ggacctcatc taccgggacc tgaagcccga gaatcttctc
     3421 atcgaccagc agggctatat tcaggtgaca gacttcggtt ttgccaagcg tgtgaaaggc
     3481 cgtactcgga ccttgtgtgg gacccctgag tacttggccc ccgagattat cctgagcaaa
     3541 ggctacaaca aggctgtgga ctggtgggct ctcggagtcc tcatctacga gatggctgct
     3601 ggttacccac ccttcttcgc tgaccagcct atccagatct atgagaaaat cgtctctggg
     3661 aaggtgcggt tcccatccca cttcagctct gacttgaagg acctgctgcg gaaccttctg
     3721 caagtggatc tcaccaagcg ctttgggaac ctcaagaacg gggtcaatga catcaagaac
     3781 cacaagtggt ttgccacgac tgactggatt gccatctatc agagaaaggt ggaagctccc
     3841 ttcataccaa agtttaaagg ccctggggac acgagtaacg ccgacgacta tgaggaggaa
     3901 gagatccggg tctccatcaa tgagaagtgt ggcaaggagt ttactgagtt ttaatgagcg
     3961 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4021 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4201 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     4261 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     4321 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     4381 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     4441 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     4501 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     4561 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     4621 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     4681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     4801 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4861 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4981 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5101 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     5161 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     5221 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5281 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5341 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     5401 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5461 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5521 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5581 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5641 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5701 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5761 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     5821 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     5881 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     5941 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6001 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6061 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6121 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     6181 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     6241 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     6301 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     6361 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     6421 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6481 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6541 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6721 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7201 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7261 ttctgtggat aaccgtatta ccgccatgca t
//
