LOCUS       pEF1-Lyn-HA-mT-VASP        5562 bp    DNA     circular      8-NOV-2017
SOURCE      
  ORGANISM  
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
            Cat. No. 631980
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12019245|
COMMENT     VNTDBDATE|-12019245|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEF1-Lyn-HA-mT-VASP|
COMMENT     VNTAUTHORNAME|Colin O'Banion|
COMMENT     VNTAUTHORTEL|919-9661146|
COMMENT     VNTAUTHOREML|cobanion@ad.unc.edu|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 3427 0 0 "") 
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COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1380 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 1397 0 3316 0 4019 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 485 0 990 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) 
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COMMENT             (CStringList "/codon_start=1" "/transl_table=11" 
COMMENT              "/product=\"neomycin phosphotransferase\"" 
COMMENT              "/protein_id=\"AAB02575.1\"" "/db_xref=\"GI:1377913\"") 1 1 1 1 
COMMENT             "3918..4712") 
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COMMENT        #12=(CFSignal (CObList) "HSV TK polyA signal" 25 0 0 4488 4506 0 
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COMMENT        #13=(CFSignal (CObList) "SV40 early Promoter" 30 0 0 3107 3375 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3567..3835") 
COMMENT        #14=(CFSignal (CObList) "f1 origin" 33 0 1 2478 2933 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2938..3393)") 
COMMENT        #15=(CFSignal (CObList) "SV40 origin" 33 0 0 3274 3409 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3734..3869") 
COMMENT        #16=(CFSignal (CObList) "pUC origin" 33 0 0 4837 5480 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5297..5940") 
COMMENT        #17=(CFSignal (CObList) "EF1a Promoter" 30 0 0 12 1346 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "12..1346") 
COMMENT        #18=(CFSignal (CObList) "HA-mTurqouise2-VASP 148-164" 21 0 0 1398 2228 0 
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COMMENT                    "pEF1-Lyn-HA-mT-VASP" 2) 5 "" 0 4) 
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COMMENT                          "DNA 'pEF1-Lyn-HA-mT-VASP'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 11/08/17 03:00PM" 1) 
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COMMENT                          "Last Modified: 11/08/17 03:00PM" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5562 bp" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 22-MAR-2010" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 3458  End: 4252" 1) 
COMMENT                                   #38=(CLinePar 
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COMMENT                                        "Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157" 
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COMMENT                                        "   3918..4712" 1) 
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COMMENT                                        "   /protein_id=<a href=\"ncbi:protein/AAB02575.1\">AAB02575.1</a>" 
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COMMENT                                        "   /db_xref=<a href=\"ncbi:sequence/1377913\">GI:1377913</a>" 
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COMMENT                                        "Start: 1398  End: 2228" 1))) 
COMMENT                                 "HA-mTurqouise2-VASP 148-164"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "SV40 early mRNA polyadenylation signal Polyadenylation signals: 1552-1557 & 1581-1586; mRNA 3' ends: 1590 & 1602" 
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COMMENT                                        "Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal Polyadenylation signals: 3659-3664 & 3672-3677" 
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COMMENT                                        "SV40 early promoter Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421; 21-bp repeats: 2425-2445, 2446-2466, & 2468-2488; Early promoter element: 2501-2507; Major transcription start points: 2497, 2535, 2541, & 2546" 
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COMMENT                                        "f1 single-strand DNA origin Packages the noncoding strand of EGFP)" 
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FEATURES             Location/Qualifiers
     CDS             3458..4252
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     polyA_signal    2381..2431
                     /vntifkey="25"
                     /label=SV40\early\polyA\signal
                     /note="SV40 early mRNA polyadenylation signal Polyadenylation signals: 1552-1557 & 1581-1586; mRNA 3' ends: 1590 & 1602"
     polyA_signal    4488..4506
                     /vntifkey="25"
                     /label=HSV\TK\polyA\signal
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal Polyadenylation signals: 3659-3664 & 3672-3677"
     promoter        3107..3375
                     /vntifkey="30"
                     /label=SV40\early\Promoter
                     /note="SV40 early promoter Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421; 21-bp repeats: 2425-2445, 2446-2466, & 2468-2488; Early promoter element: 2501-2507; Major transcription start points: 2497, 2535, 2541, & 2546"
     rep_origin      complement(2478..2933)
                     /vntifkey="33"
                     /label=f1\origin
                     /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)"
     rep_origin      3274..3409
                     /vntifkey="33"
                     /label=SV40\origin
                     /note="SV40 origin of replication"
     rep_origin      4837..5480
                     /vntifkey="33"
                     /label=pUC\origin
                     /note="pUC plasmid replication origin"
     promoter        12..1346
                     /vntifkey="30"
                     /label=EF1a\Promoter
     misc_feature    1398..2228
                     /vntifkey="21"
                     /label=HA-mTurqouise2-VASP\148-164
BASE COUNT     1237 a      1528 c      1560 g      1237 t 
ORIGIN
        1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga 
       61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc 
      121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca 
      181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
      241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
      301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
      361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
      421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc 
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg 
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 
     1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gactcagatc tcgagctcaa 
     1381 gcttcgaatt cgccaccatg ggctgcatca agagcaaggg caaggacagc gcctacccat 
     1441 acgatgttcc agattacgct atggtgagca agggcgagga gctgttcacc ggggtggtgc 
     1501 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 
     1561 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 
     1621 tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc 
     1681 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 
     1741 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 
     1801 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 
     1861 acggcaacat cctggggcac aagctggagt acaactacat cagcgacaac gtctatatca 
     1921 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg 
     1981 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 
     2041 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg 
     2101 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 
     2161 tggacgagct gtacaagagc gagcacatcg agcggcgggt gagcaacgcc ggcggccccc 
     2221 ccgcctgagg atccccgact ctagatcata atcagccata ccacatttgt agaggtttta 
     2281 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt 
     2341 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     2401 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     2461 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 
     2521 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 
     2581 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 
     2641 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 
     2701 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 
     2761 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 
     2821 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 
     2881 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg 
     2941 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 
     3001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 
     3061 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc 
     3121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 
     3181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 
     3241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
     3301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 
     3361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 
     3421 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 
     3481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 
     3541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 
     3601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 
     3661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 
     3721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 
     3781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 
     3841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 
     3901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 
     3961 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 
     4021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 
     4081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 
     4141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 
     4201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 
     4261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 
     4321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 
     4381 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact 
     4441 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag 
     4501 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 
     4561 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 
     4621 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 
     4681 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat 
     4741 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     4801 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     4861 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     4921 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     4981 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 
     5041 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     5101 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     5161 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     5221 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     5281 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     5341 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     5401 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     5461 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 
     5521 ttatcccctg attctgtgga taaccgtatt accgccatgc at 
//
