LOCUS       pRS003_(pRec-TreR)      5213 bp ds-DNA     circular     29-MAY-2017
DEFINITION  .
ACCESSION   urn.local...2c-74n6ybu                                             
SOURCE      null                                                               
FEATURES             Location/Qualifiers
     misc_feature    1707..1741
                     /label="J23106 (anderson library)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      3553..3612
                     /label="L3S1P52"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1236..1520
                     /label="cas2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    208..281
                     /label="PLTetO-1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3745..4427)
                     /label="ColE1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             2791..3552
                     /label="treR-LBD"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             2608..2790
                     /label="lacI-DNABD"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1533..1661
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             4554..5213
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2545..2545
                     /label="C to T (lacIq)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     modified_base   2761..2763
                     /label="V52A"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2508..2607
                     /label="lacI upstream region (100bp)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1776..2402
                     /label="tetR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      4432..4537
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    282..316
                     /label="sRBS (RBS calc. 4881.77)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      2403..2507
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     CDS             317..1234
                     /label="cas1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1742..1775
                     /label="sRBS (RBS calc. 633.59)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC ATTTTCGCCA
      121 GATATCGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC
      181 ACGAGGCCCT TTCGTCTTCA CCTCGAGTCC CTATCAGTGA TAGAGATTGA CATCCCTATC
      241 AGTGATAGAG ATACTGAGCA CATCAGCAGG ACGCACTGAC CGTTTAATAA ATATATACAT
      301 ATCAGGACAA CGCCTAatga cctggcttcc ccttaatccc attccactca aagatcgcgt
      361 ctccatgatc tttctgcaat atgggcagat cgatgtaata gatggcgcgt ttgtacttat
      421 cgacaagaca gggatccgca ctcatattcc tgttggctcg gttgcctgca tcatgctgga
      481 acctggtaca cgggtttcgc atgcagctgt acgcctggct gcgcaagttg gaacattgtt
      541 ggtatgggtg ggggaagcgg gcgttcgtgt ttatgcttct ggtcagcctg gaggtgcgcg
      601 ttcagataag ctgctctatc aggcaaaact tgctctggat gaagatttgc gtctgaaggt
      661 cgtacgtaaa atgtttgaac ttcggtttgg agaacctgcg cctgcccggc gctccgtaga
      721 gcaactcaga ggtatagaag gcagtcgcgt gcgggcaacc tacgcacttc tggcgaagca
      781 atacggcgtg acatggaatg gacgtcgcta cgatccgaaa gactgggaaa agggcgatac
      841 gatcaaccaa tgcattagcg ctgcaacttc ctgtttatac ggcgtaactg aagcggcgat
      901 acttgcagct ggttatgcac cagctattgg gtttgtgcat acaggaaagc ctctttcctt
      961 tgtttacgat attgcagaca tcattaaatt tgacactgtt gtaccgaaag cttttgagat
     1021 agcgcgtcgt aaccctggtg agccggaccg ggaagtccgt ttggcgtgca gggatatttt
     1081 tcgcagtagt aaaacattag ccaaattgat tccgcttata gaggacgtgc ttgccgctgg
     1141 agaaatacaa ccgccggccc cacctgaaga tgcacagcct gttgccattc cgcttcctgt
     1201 ttcactggga gatgcaggcc atcggagtag ctgaaatgag tatgttggtc gtggtcactg
     1261 aaaatgtacc tccgcgctta cgaggcagat tagccatctg gttgttggag gtacgtgcag
     1321 gggtatatgt aggtgatgta tccgcaaaaa ttcgtgaaat gatctgggaa caaatagctg
     1381 gactggcgga agaaggcaat gtagtgatgg catgggcaac gaatacggaa acgggatttg
     1441 agttccagac atttgggtta aacaggcgta ccccggtaga tttggatggt ttaaggttgg
     1501 tgtctttttt acctgtttga TAATAATCTA GACCAGGCAT CAAATAAAAC GAAAGGCTCA
     1561 GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCTACTAGAG
     1621 TCACACTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTTAT AGGTACCCGT GGATCCTCTA
     1681 GAGTCGACCT GCAGGCATGC AAGCTTTTTA CGGCTAGCTC AGTCCTAGGT ATAGTGCTAG
     1741 CCCAGCCAGA GAAACACTCT TTAACAGGGG GTAGTATGAT GTCTCGTTTA GATAAAAGTA
     1801 AAGTGATTAA CAGCGCATTA GAGCTGCTTA ATGAGGTCGG AATCGAAGGT TTAACAACCC
     1861 GTAAACTCGC CCAGAAGCTA GGTGTAGAGC AGCCTACATT GTATTGGCAT GTAAAAAATA
     1921 AGCGGGCTTT GCTCGACGCC TTAGCCATTG AGATGTTAGA TAGGCACCAT ACTCACTTTT
     1981 GCCCTTTAGA AGGGGAAAGC TGGCAAGATT TTTTACGTAA TAACGCTAAA AGTTTTAGAT
     2041 GTGCTTTACT AAGTCATCGC GATGGAGCAA AAGTACATTT AGGTACACGG CCTACAGAAA
     2101 AACAGTATGA AACTCTCGAA AATCAATTAG CCTTTTTATG CCAACAAGGT TTTTCACTAG
     2161 AGAATGCATT ATATGCACTC AGCGCAGTGG GGCATTTTAC TTTAGGTTGC GTATTGGAAG
     2221 ATCAAGAGCA TCAAGTCGCT AAAGAAGAAA GGGAAACACC TACTACTGAT AGTATGCCGC
     2281 CATTATTACG ACAAGCTATC GAATTATTTG ATCACCAAGG TGCAGAGCCA GCCTTCTTAT
     2341 TCGGCCTTGA ATTGATCATA TGCGGATTAG AAAAACAACT TAAATGTGAA AGTGGGTCTT
     2401 AAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG
     2461 TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG CCCTAGACGA AGCGGCATGC
     2521 ATTTACGTTG ACACCATCGA ATGGTGCAAA ACCTTTCGCG GTATGGCATG ATAGCGCCCG
     2581 GAAGAGAGTC AATTCAGGGT GGTGAATgtg aaaccagtaa cgttatacga tgtcgcagag
     2641 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
     2701 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
     2761 gcggcacaac aactggcggg caaacagtcg aaagtggtcg ccatcattgt tacccgcctg
     2821 gattcgttgt cagaaaatct cgccgttcaa accatgctgc cagcgttcta tgaacaaggt
     2881 tacgacccaa tcatgatgga aagtcagttt tccccgcaat tagttgccga acatttgggg
     2941 gtgctgaaac ggcgtaatat cgacggcgta gtgctgttcg gttttactgg cataacagaa
     3001 gaaatgttag cccactggca gtcatcgctg gttctgctgg cgcgtgacgc aaaaggtttt
     3061 gcttcggtct gttatgacga cgaaggggca atcaagatcc tgatgcaacg gctgtatgac
     3121 caggggcatc gtaatatcag ttatctcggc gtgccgcata gtgacgtgac gaccgggaag
     3181 cgacgtcacg aagcctacct ggcgttctgc aaagcgcata aactgcatcc cgttgccgct
     3241 ctgccaggcc ttgctatgaa gcaaggctat gagaacgtag caaaagtgat tacgcctgaa
     3301 actaccgcct tactgtgcgc aaccgacacg ctggcacttg gcgcaagtaa atacctgcaa
     3361 gagcaacgca tcgacacctt gcaactggcg agcgtcggta atacgccatt aatgaaattc
     3421 cttcatccgg agatcgtcac cgtagatcct ggttacgccg aagctggacg ccaggcggcc
     3481 tgccagttga tcgcacaggt aaccgggcgc agcgaaccgc aacaaatcat catccccgcc
     3541 accctgtcct gaGCTTTCTA ACTAAAAAGG CCTCCCAAAT CGGGGGGCCT TTTTTATTGA
     3601 TAACAAAACG CTCCTAGGCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
     3661 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
     3721 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
     3781 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     3841 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     3901 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG
     3961 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     4021 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     4081 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     4141 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     4201 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     4261 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     4321 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     4381 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGACTA GTGCTTGGAT
     4441 TCTCACCAAT AAAAAACGCC CGGCGGCAAC CGAGCGTTCT GAACAAATCC AGATGGAGTT
     4501 CTGAGGTCAT TACTGGATCT ATCAACAGGA GTCCAAGCGA GCTCGATATC AAATTACGCC
     4561 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG
     4621 CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC
     4681 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT
     4741 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA
     4801 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG
     4861 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT
     4921 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT
     4981 TTCATTGCCA TACGAAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG
     5041 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC
     5101 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA
     5161 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CAT
//