LOCUS       Exported                5836 bp ds-DNA     circular SYN 01-APR-2016
DEFINITION  PX551.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2017 from SnapGene 4.0.8
            http://www.snapgene.com
COMMENT     SECID/File created by SciEd Central, Scientific & Educational 
            Software
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=U6-sgRNA-mEGFPcamk2a"
     misc_feature    1..141
                     /label=ITR
                     /note="ITR"
     misc_signal     160..408
                     /gene="human U6 promoter"
                     /label=human U6 promoter
                     /note="/dnas_title=human U6 promoter"
     misc_feature    409..511
                     /label=sgRNA_5'camk2a
                     /note="sgRNA_5'camk2a"
     misc_feature    518..3084
                     /label=Template_mEGFPcamk2a
                     /note="Template_mEGFPcamk2a"
     misc_feature    3099..3239
                     /label=ITR
                     /note="ITR"
     rep_origin      3331..3637
                     /gene="f1_origin"
                     /label=f1_origin
                     /note="/dnas_title=f1_origin"
     promoter        4086..4114
                     /gene="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/dnas_title=AmpR_promoter"
     gene            4156..5016
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/dnas_title=Ampicillin"
     CDS             4156..5016
                     /codon_start=1
                     /gene="ORF frame 2"
                     /label=ORF frame 2
                     /note="/dnas_title=ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5171..5790
                     /gene="pBR322_origin"
                     /label=pBR322_origin
                     /note="/dnas_title=pBR322_origin"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggcctct agaaagcttg agggcctatt tcccatgatt
      181 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
      241 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      301 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      361 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc tgcctgccca
      421 gtgccaggag ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
      481 tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct gtggcagagg cctcaggagg
      541 ggccctgctg ctcagtggtg acagataggg gtgagaaagc agacagagtc attccgtcag
      601 cattctgggt ctgtttggta cttcttctca cgataaggtg gcggtgtgat atgcacaatg
      661 gctaaaaagc agggagagct ggaaagaaac aaggacagag acagaggcca agtcaaccag
      721 accaattccc agaggaagca aagaaaccat tacagagact acaaggggga agggaaggag
      781 agatgaatta gcttcccctg taaaccttag aacccagctg ttgccagggc aacggggcaa
      841 tacctgtctc ttcagaggag atgaagttgc cagggtaact acatcctgtc tttctcaagg
      901 accatcccag aatgtggcac ccactagccg ttaccatagc aactgcctct ttgccccact
      961 taatcccatc ccgtctgtta aaagggccct atagttggag gtgggggagg taggaagagc
     1021 gatgatcact tgtggactaa gtttgttcgc atccccttct ccaaccccct cagtacatca
     1081 ccctggggga acagggtcca cttgctcctg ggcccacaca gtcctgcagt attgtgtata
     1141 taaggccagg gcaaagagga gcaggtttta aagtgaaagg caggcaggtg ttggggaggc
     1201 agttaccggg gcaacgggaa cagggcgttt cggaggtggt tgccatgggg acctggatgc
     1261 tgacgaaggc tcgcgaggct gtgagcagcc acagtgccct gctcagaagc cccaagctcg
     1321 tcagtcaagc cggttctccg tttgcactca ggagcacggg caggcgagtg gcccctagtt
     1381 ctgggggcag cgcttcagca tcccagccct agttcccagc ctaaagcctc gcctgcctgc
     1441 ccagtgccag gatgtccgtg agcaagggcg aggagctctt caccggggtg gtgcccatcc
     1501 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1561 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1621 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1681 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1741 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1801 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1861 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1921 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1981 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2041 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     2101 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2161 agctgtacaa ggctaccatc acctgcaccc gattcacaga agagtaccag ctctttgagg
     2221 aactgggaaa gtgagtcaca cctcagggag tcctagagag catggggatc agtctccttg
     2281 ggcaggggga gggggcgtgt gcctatgtgg ccagagctgt cccgtgcatg cagagggagg
     2341 tgtgcagcat gatttggaag cactgtgtat gcatagggtg tccagagtta ggcgctgaca
     2401 catttatgag tccagtcagc ctggtgtgtg tgtgtgtgtg tgtgtgtgtg atggaaagga
     2461 aacagattgg tggtgtatgc acacacctat gcatataaat acacatgacc tgggttcaca
     2521 aagatatcta gaaacttggg gtttgaaagg gattttccca cttggctgga tctgagctgg
     2581 aatctatttt tgtagttgta cttagaaacc tccaatgaca aagaaagttt gccaccccct
     2641 gacctggaga tcttgtcttt agacactgtg cagagtggct agctttctcc tagcacatcc
     2701 cctccggctt ccccagatac cttccgggag caacagcttc agtacagaag gagttaatat
     2761 aaagcaatca cagtgttggg aggaggggaa ggacagtcag ccagccgggg ggtgccactg
     2821 tggcactgac tatacaaaac aactgtggtc ctttggcaaa gttgcccatc gtgttatatc
     2881 tcaggaggct gagaggagaa aggttccctc cagaagagca gcaggctaca gatataagag
     2941 aaggtttcta gatgttccca gctaggttat ggggtcctgg gttggatcag gagttggggg
     3001 agcagtttgg cagagtgccc cacggatgca tacgtgctcc taccagtcct gtttggttcc
     3061 tgctgtagcg cagtaacatg agacgtcgac gcggccgcag gaacccctag tgatggagtt
     3121 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     3181 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     3241 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     3301 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3361 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3421 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3481 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     3541 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3601 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     3661 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3721 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     3781 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3841 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3901 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     3961 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4021 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4081 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4141 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4201 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4261 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4321 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4381 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4441 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4501 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4561 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4621 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4681 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4741 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4801 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4861 ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4921 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4981 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5041 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5101 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5161 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5221 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5281 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5341 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5401 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5461 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5521 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5581 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5641 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5701 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5761 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5821 ccttttgctc acatgt
//
