LOCUS       pUG41308                2845 bp    DNA     circular     19-OCT-2017
DEFINITION  Level 0 cloning vector.
ACCESSION   urn.local...1508232382761.189
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     This file is created by Vector NTI http://www.invitrogen.com/
            ORIGDB|GenBank VNTDATE|511794019| VNTDBDATE|511794019| LSOWNER|
            VNTNAME|pICH41308| VNTAUTHORNAME|Carola Engler|
            VNTAUTHOREML|engler@icongenetics.de|
            Vector_NTI_Display_Data_(Do_Not_Edit!) (SXF (CGexDoc "pICH41308" 0
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            #1=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 2232 0 2830 1 0 "")
            #2=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 0 0 "")
            #3=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 2 1 0 2223 0 0
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            #5=(CRSite (CStringList) "SapI" "gctcttc" 9 12 2 12 0 2158 1 0 ""))
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FEATURES             Location/Qualifiers
     misc_feature    2840..3
                     /Recognition_pattern="CACNNN^GTG"
                     /label="DraIII"
                     /note="Geneious type: restriction site"
     misc_feature    4..10
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /label="SapI"
                     /note="Geneious type: restriction site"
     CDS             215..1225
                     /label="Sm/Sp no DraIII"
     mutation        1192
                     /label="G to A"
     misc_feature    1385..1999
                     /label="rep (pMB1)"
     rep_origin      1984
                     /label="ORI"
     misc_feature    complement(2162..2168)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /label="SapI"
                     /note="Geneious type: restriction site"
     misc_feature    2217..2225
                     /Recognition_pattern="CACNNN^GTG"
                     /label="DraIII"
                     /note="Geneious type: restriction site"
     misc_feature    2225..2230
                     /Recognition_pattern="GGTCTC(1/5)"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2232..2235
                     /label="AATG"
     misc_feature    complement(2238..2243)
                     /Recognition_pattern="GAAGAC(2/6)"
                     /label="BpiI"
                     /note="Geneious type: restriction site"
     misc_feature    2279..2402
                     /label="P lac prom"
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    2371
                     /label="P(LAC)"
                     /note="Geneious type: promoter prokaryotic"
     CDS             2409..2732
                     /label="ALPHA"
     misc_feature    2822..2827
                     /Recognition_pattern="GAAGAC(2/6)"
                     /label="BpiI"
                     /note="Geneious type: restriction site"
     misc_feature    2830..2833
                     /label="GCTT"
     misc_feature    complement(2835..2840)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatgttgtc ttcgcagctg gcacgacagg tttcccgact ggaaagcggg
     2281 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     2341 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     2401 aaacagctat gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc
     2461 cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2521 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2581 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2641 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     2701 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     2761 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2821 cgaagacaag ctttgagacc acgaa
//
