LOCUS pUG1287 2845 bp DNA circular 19-OCT-2017 DEFINITION Level 0 cloning vector for insert without STOP codon. ACCESSION urn.local...1508232382559.188 VERSION KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|640684654| VNTDBDATE|640684654| LSOWNER| VNTNAME|pAGM1287| VNTAUTHORNAME|Ramona Grützner| Vector_NTI_Display_Data_(Do_Not_Edit!) (SXF (CGexDoc "pAGM1287" 0 2845 (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) (CObList #0=(CFragment 3 1 0 121 0 "" 0 "" "" "" "" "" "" "pICH41155" "" 0 0 1 2222 1 2222 2463 2139 0 "" "" "" "" "" "" (CObList) (CTerm 1 0 0 "DraIII" "" 2 0 0 0 0 0 0 0 0 0) (CTerm 1 1 0 "DraIII" "" 1 0 0 0 0 0 0 0 0 0) 1 0) #1=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "PCR pICH63682 eclev7+6" "" 0 0 1 623 2223 2845 10 632 0 "" "" "" "" "" "" (CObList) (CTerm 1 0 0 "DraIII" "" 1 0 0 0 0 0 0 0 0 0) (CTerm 1 1 0 "DraIII" "" 2 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList "BsaI" "BbsI" "BsmBI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) (CMolPar 0 0 0 0 0 1 2845 0 0 0 0 0 0 0 0) (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList #2=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 2232 0 2830 1 0 "") #3=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 2232 1 2830 0 0 "") #4=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 0 0 "")) (CObList #5=(CFSignal (CObList) "Sm/Sp no DraIII" 4 0 0 215 1225 1 (CStringList) (CStringList) 1 1 1 1 "132..1142") #6=(CFSignal (CObList) "G to A" 62 0 0 1192 1192 1 (CStringList) (CStringList) 1 1 1 1 "1109..1109") #7=(CFSignal (CObList) "ORI" 33 0 1 1984 1984 1 (CStringList) (CStringList) 1 1 1 1 "complement(1901..1901)") #8=(CFSignal (CObList) "rep (pMB1)" 21 0 0 1385 1999 1 (CStringList) (CStringList) 1 1 1 1 "1302..1916") #9=(CFSignal (CObList) "ALPHA" 4 0 0 2409 2729 1 (CStringList) (CStringList) 1 1 1 1 "342..662") #10=(CFSignal (CObList) "P lac prom" 30 0 0 2279 2402 1 (CStringList) (CStringList) 1 1 1 1 "212..335") #11=(CFSignal (CObList) "P(LAC)" 30 0 0 2371 2371 1 (CStringList) (CStringList) 1 1 1 1 "304..304") #12=(CFSignal (CObList) "AATG" 21 0 0 2232 2235 0 (CStringList) (CStringList) 1 1 1 1 "") #13=(CFSignal (CObList) "TTCG" 21 0 0 2830 2833 0 (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) (CObList) (CObList) (CTextView 0 #14=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) (CObjectList #15=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pAGM1287" 2) 5 "" 0 4) #16=(CFolderPar (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) (CObjectList #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DNA 'pAGM1287'" 1) #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Foreign object. Author: Ramona Gr\374tzner. Original author: Ramona Gr\374tzner" 1) #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 10/23/12 07:57AM" 1) #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 10/23/12 07:57AM" 1) #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 2845 bp" 1) #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Constructed" 1) #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #24=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Original Source Database: GenBank" 1) #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Modification Date in the Original DB: 23-OCT-2012" 1))) "Standard Fields") #27=(CFolderPar (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) (CObjectList #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Ramona Gr\374tzner" 1))) "Author") #29=(CFolderPar (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) (CObjectList #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Ramona Gr\374tzner" 1))) "Original Author") #31=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 0 0) #32=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #33=(CFolderPar (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) (CObjectList #34=(CFolderPar (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) (CObjectList #35=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "parent position: from 2463 to 2139" 1) #36=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "original length: 2222" 1) #37=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "molecule position: from 1 to 2222" 1) #38=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #39=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " DraIII site #2" 1))) "Left Terminus") #40=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #41=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " DraIII site #1" 1))) "Right Terminus"))) "#1: FRAGMENT of pICH41155 ") #42=(CFolderPar (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) (CObjectList #43=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "parent position: from 10 to 632" 1) #44=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "original length: 623" 1) #45=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "molecule position: from 2223 to 2845" 1) #46=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #47=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " DraIII site #1" 1))) "Left Terminus") #48=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #49=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " DraIII site #2" 1))) "Right Terminus"))) "#2: FRAGMENT of PCR pICH63682 eclev7+6 "))) "Component Fragments") #50=(CFolderPar (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) (CObjectList #51=(CFolderPar (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) (CObjectList #52=(CFolderPar (CGroupPar (CParagraph 215 (3 #5# 0) 1 2 2 0 327) (CObjectList #53=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 215 End: 1225" 1) #54=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #55=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 132..1142" 1))) "Sm/Sp no DraIII") #56=(CFolderPar (CGroupPar (CParagraph 2409 (3 #9# 0) 1 2 2 0 327) (CObjectList #57=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2409 End: 2729" 1) #58=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #59=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 342..662" 1))) "ALPHA"))) "CDS (2 total)") #60=(CFolderPar (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) (CObjectList #61=(CFolderPar (CGroupPar (CParagraph 1385 (3 #8# 0) 1 2 2 0 194) (CObjectList #62=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1385 End: 1999" 1) #63=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #64=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 1302..1916" 1))) "rep (pMB1)") #65=(CFolderPar (CGroupPar (CParagraph 2232 (3 #12# 0) 1 2 2 0 194) (CObjectList #66=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2232 End: 2235" 1))) "AATG") #67=(CFolderPar (CGroupPar (CParagraph 2830 (3 #13# 0) 1 2 2 0 194) (CObjectList #68=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2830 End: 2833" 1))) "TTCG"))) "Misc. Feature (3 total)") #69=(CFolderPar (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) (CObjectList #70=(CFolderPar (CGroupPar (CParagraph 2279 (3 #10# 0) 1 2 2 0 194) (CObjectList #71=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2279 End: 2402" 1) #72=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #73=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 212..335" 1))) "P lac prom") #74=(CFolderPar (CGroupPar (CParagraph 2371 (3 #11# 0) 1 2 2 0 194) (CObjectList #75=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2371 End: 2371" 1) #76=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #77=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 304..304" 1))) "P(LAC)"))) "Promoter Prokaryotic (2 total)") #78=(CFolderPar (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) (CObjectList #79=(CFolderPar (CGroupPar (CParagraph 1984 (3 #7# 0) 1 2 2 0 194) (CObjectList #80=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1984 End: 1984 (Complementary)" 1) #81=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #82=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(1901..1901)" 1))) "ORI"))) "Replication Origin (1 total)") #83=(CFolderPar (CGroupPar (CParagraph 62 (7 62 0) 1 1 1 0 178) (CObjectList #84=(CFolderPar (CGroupPar (CParagraph 1192 (3 #6# 0) 1 2 2 0 194) (CObjectList #85=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1192 End: 1192" 1) #86=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #87=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 1109..1109" 1))) "G to A"))) "Mutation (1 total)"))) "Feature Map") #88=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #89=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 58218936 (8 0) 1 1 1 0 178) (CObjectList #90=(CGroupPar (CParagraph 0 (10 #3# 0) 1 2 2 0 180) (CObjectList #91=(CLinePar (CParagraph 0 (1 #3# 1) 1 2 2 0 191) " N1: 2232 (on complementary strand)" 1) #92=(CLinePar (CParagraph 0 (1 #3# 2) 1 2 2 0 191) " N2: 2830 " 1))))) "BbsI: 2 sites") 8 12 "GAAGACNNNNNNN" "CTTCTGNNNNNNN") #93=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 58236536 (8 0) 1 1 1 0 178) (CObjectList #94=(CGroupPar (CParagraph 0 (10 #2# 0) 1 2 2 0 180) (CObjectList #95=(CLinePar (CParagraph 0 (1 #2# 1) 1 2 2 0 191) " N1: 2232 " 1) #96=(CLinePar (CParagraph 0 (1 #2# 2) 1 2 2 0 191) " N2: 2830 (on complementary strand)" 1))))) "BsaI: 2 sites") 7 11 "GGTCTCNNNNNN" "CCAGAGNNNNNN") #97=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 58232120 (8 0) 1 1 1 0 178) (CObjectList)) "BsmBI: 0 sites") 7 11 "CGTCTCNNNNNN" "GCAGAGNNNNNN"))) "Restriction/Methylation Map")))) (CGraphView (CWStyleSheet (CObjectList #98=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 "@N (@S)" 0) #99=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 "@N" 0) #100=(CWidgetStyle "Molecule Label 2" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 "@L bp" 0) #101=(CWidgetStyle "Molecule Label 1" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 "@N" 0) #102=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #103=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #104=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) #105=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 (LOGSHAPE 1 1.9 0 0 0)) #106=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 (LOGSHAPE 1 1.9 0 0 0)) #107=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 (LOGSHAPE 1 1.9 0 0 0)) #108=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 5 "@N (@S)" 0) #109=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 1 2 "?" 0) #110=(CWidgetStyle "Mark 1" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 0) #111=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 1 65535 "@N (@H)" 0) #112=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 "@F bp (molecule @L bp)" 0) #113=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "Fragment of @N" 0) #114=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 8388608 5) 0 0 0) #115=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 0 0) #116=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 0) #117=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) #118=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) #119=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) #120=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) #121=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) #122=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) #123=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) #124=(CWidgetStyle "Void" 0 0 0 0 0) #125=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 1 3 "@T @N " 0) #126=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) #127=(CWidgetStyle "Annotation" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 0 0) #128=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 "@N" 0) #129=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 0 0 0) #130=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 "@N" 0) #131=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 0 1 65535 "@N" 0) #132=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 "@N" 0) #133=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 16777113 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #134=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #135=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 1 1 "@N" 0) #136=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 5 "@N (@S)" 0) #137=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -2706) 1 1 0 1 1 (mapper: 19.1473 -17.8537 40.0378 40.0378 0.01 10 -1 2845 2845 1 0 0) #138=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 745434985 100) (CObjectList #139=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -102 100) (CObjectList #140=(CAxis (CWideLine (CWidget 0 (0 0) 1 2 0 0 #104# 57800876 0) (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 6.27639 6.27439 1 0.0214037) 0.220499) #141=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #101# 2082603430 100) (LOGPEN 0 0 13408563) 1 (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 1.74233 0.833333 0 "pAGM1287" "@N" 1 0 0.871165 0 -10 6.16917 1.39868 Nil) #142=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #100# 1565648951 100) (LOGPEN 0 0 13408563) 1 (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233 0.555556 0 "2845 bp" "@L bp" 16 0 -0.871165 0 -10.8 2.59755 0.799245 Nil)) (CObjectList)) #143=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -451 100) (CObjectList #144=(CGroupWidget (CWidget 4 (7 4 0) 1 2 0 0 Nil -172 100) (CObjectList #145=(CWideArrow (CWideLine (CWidget 0 (3 #5# 0) 1 2 0 0 #102# 0 100) (LOGPEN 0 0 3355545) 1 (LOGBRUSH 0 6724095 0) 1 3.57208 5.80132 1 0.082322) 0.8 1.8 0) #146=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 9 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "Sm/Sp no DraIII" "@N" 1 16.768 0 0 -10 6.79358 1.04901 #145#) #147=(CWideArrow (CWideLine (CWidget 0 (3 #9# 0) 1 2 0 0 #102# 0 100) (LOGPEN 0 0 3355545) 1 (LOGBRUSH 0 6724095 0) 1 0.255778 0.963579 1 0.082322) 0.8 1.8 0) #148=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 1701603182 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "ALPHA" "@N" 1 -10.5428 10.454 0 -10 3.09707 1.04901 #147#)) (CObjectList)) #149=(CGroupWidget (CWidget 21 (7 21 0) 1 2 0 0 Nil -875 100) (CObjectList #150=(CWideArrow (CWideLine (CWidget 0 (3 #8# 0) 1 2 0 0 #103# 0 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334 0) 1 1.86542 3.22148 1 0.082322) 0.8 1.8 0) #151=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 1565649083 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "rep (pMB1)" "@N" 1 -11.4053 -10.454 0 -10 4.77049 1.04901 #150#) #152=(CScratch (CWidget 0 (3 #12# 0) 1 2 0 0 #107# 1 100) (LOGPEN 0 8 13395507) 10 1 1.35386 1.9 0.082322 1) #153=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 1869766979 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "AATG" "@N" 1 -14.6448 1.74233 0 -10 2.47266 1.04901 #152#) #154=(CScratch (CWidget 0 (3 #13# 0) 1 2 0 0 #107# 1 100) (LOGPEN 0 8 13395507) 10 1 0.0352798 1.9 0.082322 1) #155=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 2082603830 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "TTCG" "@N" 1 -7.61481 12.1963 0 -10 2.29783 1.04901 #154#)) (CObjectList)) #156=(CGroupWidget (CWidget 30 (7 30 0) 1 2 0 0 Nil -317 100) (CObjectList #157=(CWideArrow (CWideLine (CWidget 0 (3 #10# 0) 1 2 0 0 #115# 0 100) (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 0.976809 1.25023 1 0.082322) 0.8 1.8 0) #158=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 9 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "P lac prom" "@N" 1 -14.6832 6.96932 0 -10 4.77049 1.04901 #157#) #159=(CPromoterArrow (CLine (CWidget 0 (3 #11# 0) 1 2 0 0 #121# 0 100) (LOGPEN 0 4 128) 6 1.16464 0.960102 1.04737) 0.4 0.082322 1.08464 1.8 1.8 0) #160=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 1212369264 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "P(LAC)" "@N" 1 -13.2865 8.71165 0 -10 2.99717 1.04901 #159#)) (CObjectList)) #161=(CGroupWidget (CWidget 33 (7 33 0) 1 2 0 0 Nil -1154 100) (CObjectList #162=(CScratch (CWidget 0 (3 #7# 0) 1 2 0 0 #107# 1 100) (LOGPEN 0 8 13395507) 10 1 1.9007 1.9 0.082322 1) #163=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 53 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "ORI" "@N" 1 -13.7187 -5.22699 0 -10 1.67342 1.04901 #162#)) (CObjectList)) #164=(CGroupWidget (CWidget 62 (7 62 0) 1 2 0 0 Nil -521 100) (CObjectList #165=(CScratch (CWidget 0 (3 #6# 0) 1 2 0 0 #107# 0 100) (LOGPEN 0 8 13395507) 10 1 3.64705 1.9 0.082322 1) #166=(CLabel (CWidget 0 (0 0) 1 2 0 0 #99# 50 100) (LOGPEN 0 0 0) 1 (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "G to A" "@N" 1 8.56483 -12.1963 0 -10 2.7474 1.04901 #165#)) (CObjectList))) (CObjectList)) #167=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -386 100) (CObjectList #168=(CGroupWidget (CWidget 2 (10 #2# 0) 1 2 0 0 Nil -596 100) (CObjectList #169=(CScratch (CWidget 1 (1 #2# 1) 1 2 0 0 #106# 268698077 100) (LOGPEN 0 6 10053171) 8 1 1.35386 1.9 0.082322 1) #170=(CLabel (CWidget 0 (0 0) 1 2 0 0 #98# 1565650735 100) (LOGPEN 0 0 13408563) 1 (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233 0.555556 0 "{\\i Bsa}I (2232)" "@N (@S)" 5 -15.5372 3.48466 0 -10 3.62158 0.799245 #169#) #171=(CScratch (CWidget 2 (1 #2# 2) 1 2 0 0 #106# 77791696 100) (LOGPEN 0 6 10053171) 8 1 0.0352798 1.9 0.082322 1) #172=(CLabel (CWidget 0 (0 0) 1 2 0 0 #98# 2082603580 100) (LOGPEN 0 0 13408563) 1 (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233 0.555556 0 "{\\i Bsa}I (2830)" "@N (@S)" 5 -3.60765 13.9386 0 -10 3.79641 0.799245 #171#)) (CObjectList)) #173=(CGroupWidget (CWidget 2 (10 #3# 0) 1 2 0 0 Nil -1084 100) (CObjectList #174=(CScratch (CWidget 1 (1 #3# 1) 1 2 0 0 #106# 53498652 100) (LOGPEN 0 6 10053171) 8 1 1.35386 1.9 0.082322 1) #175=(CLabel (CWidget 0 (0 0) 1 2 0 0 #98# 1717924947 100) (LOGPEN 0 0 13408563) 1 (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233 0.555556 0 "{\\i Bbs}I (2232)" "@N (@S)" 5 -15.6274 5.22699 0 -10 3.52167 0.799245 #174#) #176=(CScratch (CWidget 2 (1 #3# 2) 1 2 0 0 #106# 1 100) (LOGPEN 0 6 10053171) 8 1 0.0352798 1.9 0.082322 1) #177=(CLabel (CWidget 0 (0 0) 1 2 0 0 #98# 13 100) (LOGPEN 0 0 13408563) 1 (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233 0.555556 0 "{\\i Bbs}I (2830)" "@N (@S)" 5 -3.70942 15.681 0 -10 3.69651 0.799245 #176#)) (CObjectList))) (CObjectList)) #178=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -247 100) (CObjectList) (CObjectList)) #179=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -665 100) (CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 103 (CStringList) 2147483648 351057141 (CObList))). FEATURES Location/Qualifiers CDS 215..1225 /label="Sm/Sp no DraIII" mutation 1192 /label="G to A" misc_feature 1385..1999 /label="rep (pMB1)" rep_origin 1984 /label="ORI" misc_feature 2225..2230 /Recognition_pattern="GGTCTC(1/5)" /label="BsaI" /note="Geneious type: restriction site" misc_feature 2232..2235 /label="AATG" misc_feature complement(2238..2243) /Recognition_pattern="GAAGAC(2/6)" /label="BbsI" /note="Geneious type: restriction site" misc_feature 2279..2402 /label="P lac prom" /note="Geneious type: promoter prokaryotic" misc_feature 2371 /label="P(LAC)" /note="Geneious type: promoter prokaryotic" CDS 2409..2729 /label="ALPHA" misc_feature 2822..2827 /Recognition_pattern="GAAGAC(2/6)" /label="BbsI" /note="Geneious type: restriction site" misc_feature 2830..2833 /label="TTCG" misc_feature complement(2835..2840) /Recognition_pattern="GGTCTC(1/5)" /label="BsaI" /note="Geneious type: restriction site" ORIGIN 1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac 241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg 301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg 361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac 421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt 481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc 541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt 601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc 661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat 721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga 781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac 841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc 901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc 961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag 1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct 1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga 1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga 1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat 1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact 2221 ctgtggtctc aaatgttgtc ttcgcagctg gcacgacagg tttcccgact ggaaagcggg 2281 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 2341 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2401 aaacagctat gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc 2461 cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 2521 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 2581 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc 2641 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca 2701 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac 2761 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga 2821 cgaagacaat tcgtgagacc acgaa //