LOCUS       pTol1-UAS-zactMe        6937 bp    DNA     linear       25-JUN-2015
FEATURES             Location/Qualifiers
     misc_feature    4653..4681
                     /note="29 bp repeated pUC57 sequence"
     misc_feature    4682..4704
                     /note="unknown sequence"
     misc_feature    4705..4733
                     /note="29 bp repeated sequence"
     source          402..4569
                     /dnas_title="pTol1-UAS-zactMek2b-2a-tdTomato"
                     /organism="Oryzias latipes"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:8090"
     mobile_element  402..4569
                     /mobile_element_type="transposon:Tol-tyr"
     repeat_region   402..423
                     /standard_name="terminal inverted repeat"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="terminal repeat; terminal repeat of Tol-tyr"
                     /citation=[1]
                     /rpt_type=inverted
     misc_feature    410..566
                     /note="Tol1 left arm"
     misc_feature    628..648
                     /note="attR4"
     misc_feature    4261..4456
                     /note="SV40 UTR/pA from pCS2"
     misc_feature    4219..4239
                     /note="attR3"
     repeat_region   4556..4569
                     /standard_name="terminal inverted repeat"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="terminal repeat of Tol-tyr"
                     /citation=[1]
                     /rpt_type=inverted
     misc_feature    4464..4569
                     /note="Tol1 right arm"
     misc_feature    1133..1156
                     /note="attB1"
     misc_feature    1198..1236
                     /note="myc tag 1"
     misc_feature    1237..1275
                     /note="myc tag 2"
     misc_feature    1276..1314
                     /note="myc tag 3"
     misc_feature    1315..1353
                     /note="myc tag 4"
     misc_feature    1354..1392
                     /note="myc tag 5"
     misc_feature    1417..1458
                     /note="myc tag 6"
     misc_feature    1483..2667
                     /note="full length of zMap2k2b (ENSDART00000099839)"
     misc_feature    2143..2145
                     /note="S221D"
     misc_feature    2155..2157
                     /note="S225D"
     misc_feature    2695..2719
                     /note="attB2"
     misc_feature    2722..2787
                     /note="2A"
     misc_feature    2788..4215
                     /note="tdTomato (2X)"
     misc_feature    754..770
                     /note="UAS"
     misc_feature    778..794
                     /note="UAS"
     misc_feature    806..822
                     /note="UAS"
     misc_feature    831..847
                     /note="UAS"
     misc_feature    854..870
                     /note="UAS"
     misc_feature    878..894
                     /note="UAS"
     misc_feature    906..922
                     /note="UAS"
     misc_feature    931..947
                     /note="UAS"
     misc_feature    954..970
                     /note="UAS"
     misc_feature    1157..1164
                     /note="kozak"
     misc_feature    4611..4616
                     /note="NO BamHI site here"
     misc_feature    4601..4606
                     /note="NO XbaI site here"
ORIGIN      
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCCTTTAGCc agtagcggtt
      421 ctaggcacgg gccgtccggg cggtggcctg gggcggaaaa ctgaaggggg gcggcaccgg
      481 cggctcagcc ctttgtaata tattaatatg caccactatt ggtttactta tgtcacagtt
      541 tgtaagtttg taacagcctg aacctggccg cgcgccattg ggatggaacc ctcgaggggc
      601 ccgctagcga tatccctgca gggctatcaa ctttgtatag aaaagttgGT AATACGACTC
      661 ACTATAGGGC GAATTGGGTA CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTAG
      721 GCCTCCAAGG CGGAGTACTG TCCTCCGGGC TGGCGGAGTA CTGTCCTCCG GCAAGGTCGG
      781 AGTACTGTCC TCCGACACTA GAGGTCGGAG TACTGTCCTC CGACGCAAGG CGGAGTACTG
      841 TCCTCCGGGC TXGCGGAGTA CTGTCCTCCG GCAAGGTCGG AGTACTGTCC TCCGACACTA
      901 GAGGTCGGAG TACTGTCCTC CGACGCAAGG CGGAGTACTG TCCTCCGGGC TGGCGGAGTA
      961 CTGTCCTCCG GCAAGGGTCG ACTCTAGAGG GTATATAATG GATCCCATCG CGTCTCAGCC
     1021 TCACTTTGAG CTCCTCCACA CGAATTCCTG CAGCCCGGGG GATCCACTAG TTCTAGAGCG
     1081 GCCGCCACCG CGGTGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTcaagtttg
     1141 tacaaaaaag caggctCCGC CACCATGCTT TTTGCAGGAT CCCATCGATT taaagctatg
     1201 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
     1261 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
     1321 caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag
     1381 gacttgaatg aaatggagag cttgggcgac ctcaccatgg agcaaaagct catttctgaa
     1441 gaggacttga atttagagat ctcGAATTCA AGGCCTCTCG AGATGGCTCC CAAAAAGAGG
     1501 CCAGATCCTA TAGTTATAAC TGGAAGCGGA GAAGCACAGA CGACCTCCAC GCCCATAGAT
     1561 GCTGCATCAG AAGCGAATCT TGAAGCCCTG CAGAAGAAGC TGGATGAGCT GGATTTGGAC
     1621 GAGCAGCAGA AGAAGCGTCT GGAAGCTTTC CTCACGCAGA AAGCCAATCT GGGAGAGCTG
     1681 AAGGACGATG ATTTCCAGCA TATCTGTGAA CTGGGAGCTG GAAACGGAGG AGTGGTGAAT
     1741 AAAGTGTGCC ACAAACCTTC AGGTCTCGTC ATGGCCCGGA AGTTAATTCA CCTGGAGATC
     1801 AAGCCGGCCA TCAGGCATCA GATCATCAGA GAGCTGCAGG TCTTGCATGA GTGTAATTCA
     1861 CCATACATCG TGGGTTTTTA CGGCGCATTT TACAGCGATG GCGAGATTAG TATCTGTATG
     1921 GAGAACATGG ACGGCGGATC GTTGGATCAG GTCCTGAAAG AGGCTCGCAG AATCCCAGAG
     1981 GAGATTTTGG GAAAAGTCAG CATAGCTGTT TTAAGAGGTC TTGCTTACCT TCGAGAAAAG
     2041 CACCAGATTA TTCACAGAGA TGTGAAACCC TCAAATATTC TGGTAAACTG TCGCGGGGAG
     2101 ATCAAGCTGT GTGACTTCGG TGTGAGCGGG CAGCTGATCG ACgacATGGC CAACgacTTT
     2161 GTAGGAACAC GCTCATACAT GTCGCCGGAG CGGTTGCAGG GCACTCATTA CTCGGTTCAG
     2221 TCAGACGTGT GGAGTATGGG TCTCTCTCTG GTGGAGCTCG CCATCGGCCG ATACCCTATT
     2281 CCTCCACCTG ACGCCAAGGA GCTGGAGGGC ATCTTCGGCA GGGCACTGAT GGATGTTGGA
     2341 GATTCAGAAA CACACAGCAC GTCTCCTCGA CCCAGACCAC CTGGCAGACC CATCAGCGGT
     2401 CATGGGCCGG TCATGGCTAT ATTTGAGCTG CTGGATTATA TAGTCAATGA GCCTCCTCCT
     2461 AAACTGCCTC ACGGCGTCTT CACTCCTGAT TTCCAGGACT TTGTGACCAA CTGTCTTATT
     2521 AAAAACCCTG CAGACAGAGC GGACCTGAAG ATGCTGATGA ATCACACATT TATCAAACGC
     2581 GCCGAAGTGG AGGAGATGGA CTTTGCTGGA TGGTTGTGTA AAACAATGGG TCTCAATCAG
     2641 CCCAGCACAC CCACACAAAC AACTGAAtct agaACTATAG TGAGTCGTAT TACGcaccca
     2701 gctttcttgt acaaagtgga cGGATCCGGA GCCACGAACT TCTCTCTGTT AAAGCAAGCA
     2761 GGAGACGTGG AAGAAAACCC CGGTCCTatg gtgagcaagg gcgaggaggt catcaaagag
     2821 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
     2881 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     2941 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
     3001 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
     3061 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
     3121 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
     3181 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     3241 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
     3301 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
     3361 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     3421 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc
     3481 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc
     3541 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     3601 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3661 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     3721 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     3781 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     3841 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     3901 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     3961 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     4021 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     4081 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     4141 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     4201 gacgagctgt acaagTAAca actttattat acatagttga taaGGCGCGC CAGGCCTGTA
     4261 GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA
     4321 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
     4381 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
     4441 GTGTGGGAGG TTTTTTAATT aaaacatttc agttgacgaa gacaaacaaa gttctgttgt
     4501 gactatgggg ggggggggcg cctggggatg gtctcgcccg gggagtaatt cagggtagaa
     4561 ccgccactgG CTAAAGGGAG CTCGGTACCT CGCGAATGCA TCTAGATATC GGATCCCGGG
     4621 CCCGTCGACT GCAGAGGCCT GCATGCaagc ttGCGTAATC ATGGTCATAG CTGTTTCCTG
     4681 TATCCCAATG GCGCGCCGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
     4741 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
     4801 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG
     4861 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
     4921 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
     4981 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
     5041 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     5101 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     5161 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     5221 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     5281 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     5341 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     5401 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     5461 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     5521 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
     5581 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     5641 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     5701 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     5761 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     5821 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     5881 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     5941 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     6001 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     6061 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     6121 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     6181 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     6241 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     6301 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     6361 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     6421 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     6481 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     6541 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     6601 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     6661 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     6721 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     6781 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     6841 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA
     6901 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTC
//

