LOCUS       pCDH-EF1-extGluc        8125 bp    DNA     circular UNA 12-JUN-2018
DEFINITION  Ligation of PCRprod2 cut SalI NsiI into
            pCDH-EF1-extGluc-T2A-mKate-T2A-NGFR cut SalI NsiI.
ACCESSION   urn.local...p-94rwtvs
VERSION     urn.local...p-94rwtvs
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    7..234
                     /standard_name="RSV"
     misc_feature    235..414
                     /standard_name="5'LTR"
     misc_feature    1076..1308
                     /standard_name="RRE"
     gene            1798..1916
                     /standard_name="cPPT"
     gene            1928..2473
                     /standard_name="EF1"
     gene            2452..2469
                     /standard_name="Primer 196 s"
     gene            2498..3295
                     /standard_name="extGLuc"
     gene            3311..3364
                     /standard_name="T2A"
     gene            3334..3352
                     /standard_name="Primer 198"
     gene            3371..4201
                     /standard_name="NGFR"
     gene            4220..4810
                     /standard_name="WPRE"
     gene            4223..4247
                     /standard_name="Primer 197"
     gene            4882..5115
                     /standard_name="3'^LTR"
     misc_feature    5327..5473
                     /standard_name="SV40 ORI"
     gene            complement(5843..6516)
                     /standard_name="pUC ORI"
     gene            complement(6661..7521)
                     /standard_name="AmpR"
ORIGIN      
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gctctcccag tgactgccct
     2521 actgcttccc ctagcgcttc tcctgcatgc caagcccacc gagaacaacg aagacttcaa
     2581 catcgtggcc gtggccagca acttcgcgac cacggatctc gatgctgacc gcgggaagtt
     2641 gcccggcaag aagctgccgc tggaggtgct caaagagatg gaagccaatg cccggaaagc
     2701 tggctgcacc aggggctgtc tgatctgcct gtcccacatc aagtgcacgc ccaagatgaa
     2761 gaagttcatc ccaggacgct gccacaccta cgaaggcgac aaagagtccg cacagggcgg
     2821 cataggcgag gcgatcgtgg acattcctga gattcctggg ttcaaggact tggagccaat
     2881 ggagcagttc atcgcacagg tcgatctgtg tgtggactgc acaactggct gcctcaaagg
     2941 gcttgccaac gtgcagtgtt ctgacctgct caagaagtgg ctgccgcaac gctgtgcgac
     3001 ctttgccagc aagatccagg gccaggtgga caagatcaag ggggccggtg gtgacttagc
     3061 ggccgcaccc accacgacgc cagcgccgcg accaccaacc ccggcgccca cgatcgcgtc
     3121 gcagccccta tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac
     3181 gagggggctg gacttcgcct gtgatatcta catctgggcg cccctggccg ggacttgtgg
     3241 ggtccttctc ctgtcactgg ttatcaccct ttactgcaac cacaggcgga ttcaaggatc
     3301 cgcggccgct gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     3361 ccctatgcat atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct
     3421 gttgctgctt ctgggggtgt cccttggagg tgccaaggag gcatgcccca caggcctgta
     3481 cacacacagc ggtgagtgct gcaaagcctg caacctgggc gagggtgtgg cccagccttg
     3541 tggagccaac cagaccgtgt gtgagccctg cctggacagc gtgacgttct ccgacgtggt
     3601 gagcgcgacc gagccgtgca agccgtgcac cgagtgcgtg gggctccaga gcatgtcggc
     3661 gccgtgcgtg gaggccgacg acgccgtgtg ccgctgcgcc tacggctact accaggatga
     3721 gacgactggg cgctgcgagg cgtgccgcgt gtgcgaggcg ggctcgggcc tcgtgttctc
     3781 ctgccaggac aagcagaaca ccgtgtgcga ggagtgcccc gacggcacgt attccgacga
     3841 ggccaaccac gtggacccgt gcctgccctg caccgtgtgc gaggacaccg agcgccagct
     3901 ccgcgagtgc acacgctggg ccgacgccga gtgcgaggag atccctggcc gttggattac
     3961 acggtccaca cccccagagg gctcggacag cacagccccc agcacccagg agcctgaggc
     4021 acctccagaa caagacctca tagccagcac ggtggcaggt gtggtgacca cagtgatggg
     4081 cagctcccag cccgtggtga cccgaggcac caccgacaac ctcatccctg tctattgctc
     4141 catcctggct gctgtggttg tgggccttgt ggcctacata gccttcaaga ggtggaactg
     4201 aagctgaatc taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4261 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4321 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4381 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4441 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4501 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4561 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4621 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4681 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4741 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4801 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     4861 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
     4921 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4981 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5041 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5101 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5161 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5221 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5281 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5341 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5401 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5461 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
     5521 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5581 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5641 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5701 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5761 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5821 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6301 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6421 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6481 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6661 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6721 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6781 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6841 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6901 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6961 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7021 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7081 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7141 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7201 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7261 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7321 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7381 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7441 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7501 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7561 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7621 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     7681 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     7741 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     7801 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     7861 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     7921 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     7981 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     8041 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     8101 taaaacgacg gccagtgcca agctg
//
