LOCUS New_DNA 6352 bp ds-DNA linear 07-FEB-2017 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT GPP4-MLNR-NNES-eLOV-TEVSM-V5-no WPRE from 1 to 6418 COMMENT GPP3-AVPR1B-NNES-eLOV-TEVSM-V5-no WPRE from 1 to 6454 COMMENT GPP2-GPBA-NNES-eLOV-TEVSM-V5-no WPRE from 1 to 6172 COMMENT GPP1-DRD4-NNES-eLOV-TEVSM(ENLYFQ-M)-GAL4-V5-no WPRE from 1 to 6439 COMMENT APP14-beta1-AR-NNES-eLOV-TEVSM(ENLYFQ-M)-GAL4-V5-no WPRE from 1 to 6613 COMMENT APP6-beta2-AR-NNES-eLOV-TEVSM(ENLYFQ-M)-GAL4-V5-no WPRE (1) from 1 to 6421 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature join(1..122,144..151) /label=ITR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2486..2491 /label=BamHI /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(2039..2065,2069..2113,2117..2380,2384..2389) /label=asLOV2 /ApEinfo_fwdcolor=#f746ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 774..790 /label=T7 promoter primer /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2408..2410 /label=G528A /ApEinfo_fwdcolor=#ffe19f /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3616..3756 /label=ITR(1) /ApEinfo_label=ITR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2420..2422 /label=I130V /ApEinfo_fwdcolor=#ffe950 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(3296..3303,3305..3309,3311..3315,3317..3336) /label=V5 tag /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 806..807 /label=NotI /ApEinfo_fwdcolor=#ff8040 /ApEinfo_revcolor=#ff8040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3356..3587 /label=PolyA /ApEinfo_fwdcolor=#ff2549 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2024..2029 /label=nhe1 /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2030..2035 /label=asLOV2(1) /ApEinfo_label=asLOV2 /ApEinfo_fwdcolor=#f746ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 172..729 /label=CMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2036..2038 /label=L2R /ApEinfo_fwdcolor=#ffe85a /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2492..2515 /label=Flag /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1982..2017 /label=NNES /ApEinfo_fwdcolor=#fffb76 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2438..2440 /label=N538E /ApEinfo_fwdcolor=#ff9e3f /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(2390..2407,2411..2419,2423..2437) /label=asLOV2(2) /ApEinfo_label=asLOV2 /ApEinfo_fwdcolor=#f746ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2441..2458 /label=asLOV2(3) /ApEinfo_label=asLOV2 /ApEinfo_fwdcolor=#f746ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2516..3295 /label=GAL4 /ApEinfo_fwdcolor=#f026ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2066..2068 /label=N12S /ApEinfo_fwdcolor=#ffd85f /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2114..2116 /label=A28V /ApEinfo_fwdcolor=#ffe04d /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3344..3349 /label=EcoRI /ApEinfo_fwdcolor=#46ff6e /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2459..2479 /label=TEVcs ENLYFQL /ApEinfo_fwdcolor=#76cbff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3341..3343) /label=double stop /ApEinfo_fwdcolor=#800080 /ApEinfo_revcolor=#800080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(2390..2407,2411..2419,2423..2437) /label=asLOV2 Ja helix /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2381..2383 /label=H117R /ApEinfo_fwdcolor=#ffe369 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2024..2029 /label=NheI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2441..2458 /label=asLOV2 Ja helix(1) /ApEinfo_label=asLOV2 Ja helix /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 812..1981 /label=NMBR /ApEinfo_fwdcolor=#ff8b0c /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 agCGCAAATG GGCGGTAGGC GTGgggttcc tgcggccgca cgcgtTGTAC Atagtaatca 181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga ctatttacgg 361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg 541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc 601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt 661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 721 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg 781 actcactata gggagaccca agcttgccac cATGCCCTCC AAGTCTCTGT CAAATCTGAG 841 TGTCACCACC GGAGCTAATG AGAGTGGATC TGTTCCTGAG GGATGGGAAA GGGATTTCCT 901 TCCAGCTAGC GATGGGACCA CAACAGAGCT TGTGATCCGG TGCGTGATCC CCTCACTCTA 961 CTTGCTGATC ATTACAGTCG GGCTTCTCGG CAACATAATG CTCGTTAAGA TTTTCATAAC 1021 TAATTCAGCC ATGCGGTCCG TTCCCAACAT CTTTATCAGT AACCTCGCTG CAGGTGATCT 1081 GCTCCTGCTT CTGACCTGCG TACCTGTGGA CGCATCCCGC TACTTTTTTG ATGAATGGAT 1141 GTTTGGAAAG GTTGGGTGTA AGCTGATCCC TGTCATCCAG CTCACGTCAG TGGGGGTGAG 1201 TGTGTTCACG CTTACTGCCC TCTCTGCAGA TAGATACCGA GCGATCGTAA ACCCCATGGA 1261 TATGCAGACG TCCGGCGCTC TTCTCCGGAC TTGCGTGAAG GCAATGGGAA TATGGGTGGT 1321 GTCAGTGTTG CTGGCTGTTC CCGAGGCCGT GTTTTCAGAG GTTGCTAGGA TCTCTTCACT 1381 GGATAATAGT TCATTCACGG CCTGTATTCC ATATCCCCAG ACAGACGAGC TGCACCCTAA 1441 GATCCACTCC GTACTTATAT TTTTGGTCTA TTTTCTGATC CCCCTTGCAA TCATCTCAAT 1501 CTATTACTAC CACATCGCAA AAACATTGAT CAAATCCGCC CATAACCTCC CCGGGGAGTA 1561 CAATGAACAT ACAAAGAAGC AGATGGAGAC CAGGAAAAGG CTCGCCAAGA TCGTCCTTGT 1621 TTTCGTTGGG TGCTTTATCT TCTGCTGGTT TCCCAATCAC ATACTGTATA TGTACCGGAG 1681 TTTTAACTAT AATGAGATTG ATCCCTCACT CGGACATATG ATTGTGACCC TCGTGGCCCG 1741 GGTGCTCTCC TTCGGGAATA GCTGTGTCAA CCCCTTCGCG CTGTACCTGC TCTCCGAGTC 1801 TTTTCGCCGA CACTTCAATT CACAGCTCTG CTGTGGGAGA AAAAGCTACC AGGAACGAGG 1861 AACATCTTAT CTGCTTTCAT CTAGCGCCGT GCGGATGACA TCCCTGAAGA GTAACGCGAA 1921 GAACATGGTG ACGAACTCAG TCCTCCTGAA TGGGCATTCC ATGAAGCAGG AGATGGCCCT 1981 GGAGCTGGCC GAGAAGCTGG CCGGCCTGGA CATCAACGGT GGAgctagcG GCAGCcggGC 2041 GACCACCCTG GAACGTATTG AAAAGagcTT TGTGATTACC GATCCGCGTC TGCCGGATAA 2101 CCCGATTATT TTTgtgAGCG ATAGCTTTCT GCAGCTGACC GAATATAGCC GTGAAGAAAT 2161 TCTGGGCCGT AACTGCCGTT TTCTGCAGGG CCCGGAAACC GATCGTGCGA CCGTGCGTAA 2221 AATTCGTGAT GCGATTGATA ACCAGACCGA AGTGACCGTG CAGCTGATTA ACTATACCAA 2281 AAGCGGCAAA AAATTTTGGA ACCTGTTTCA TCTGCAGCCG ATGCGTGATC AGAAAGGCGA 2341 TGTGCAGTAT TTTATTGGCG TGCAGCTGGA TGGTACCGAA cgtGTGCGTG ATGCGGCGGA 2401 ACGTGAAGCT GTGATGCTGg tcAAGAAAAC CGCGgagGAA atcgacgaag ccgctaagGA 2461 AAATCTCTAC TTCCAGATGG GCGGAggatc cGACTACAAG GATGACGACG ATAAGaagct 2521 actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa 2581 agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa 2641 aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag 2701 actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat 2761 ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa 2821 taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc taacattgag 2881 acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca 2941 gttgactgta tcggccaatt ttaatcaaag tgggaatatt gctgatagct cattgtcctt 3001 cactttcact aacagtagca acggtccgaa cctcataaca actcaaacaa attctcaagc 3061 gctttcacaa ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac 3121 ggctagtaaa attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca 3181 aactgcgtat aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga 3241 tgtatataac tatctattcg atgatgaaga taccccacca aacccaaaaa aagagGGCAA 3301 GCCGATCCCT AACCCtCTGC TGGGCCTGGA CAGCACTtag TAAGAATTCc tcgagCTAGA 3361 GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC 3421 CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG 3481 GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG 3541 GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGAggt aaccacgtgc 3601 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc 3661 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg 3721 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc 3781 ttacgcatct gtgcggtatt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt 3841 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 3901 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 3961 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 4021 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga 4081 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 4141 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg 4201 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 4261 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc 4321 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 4381 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 4441 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 4501 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 4561 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 4621 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 4681 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 4741 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 4801 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 4861 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 4921 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 4981 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 5041 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 5101 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 5161 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 5221 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 5281 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 5341 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc 5401 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 5461 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 5521 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 5581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 5641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 5701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 5761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 5821 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 5881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 5941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 6001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 6061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 6121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 6181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 6241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 6301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt //