LOCUS       pXL-BACII-              6637 bp ds-DNA     circular SYN 14-Aug-2014
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     Olena Riabinina, Potter Lab, Johns Hopkins University School of
            Medicine
FEATURES             Location/Qualifiers
     misc_feature    3..459
                     /note=f1 origin
     LTR             746..808
                     /note=3'  Terminal Repeat piggyBAC
     misc_feature    1022..2287
                     /note=3xP3-ECFP
     promoter        1059..1099
                     /note=3xP3
     gene            1301..2020
                     /note=ECFP gene
     enhancer        2330..2611
                     /note=15xQUAS
     TATA_signal     2655..2928
                     /note=hsp70 TATA minimal promoter
     terminator      2986..3683
                     /note=SV40 terminator
     LTR             3963..3997
                     /note=5'  Terminal Repeat piggyBAC
     gene            complement(4492..4614)
                     /note=alpha lacZ gene
     rep_origin      complement(4708..5648)
                     /note=ColE1 ori
     gene            complement(5651..6508)
                     /note=Amp resistance gene
BASE COUNT      1832 A      1600 C      1572 G      1633 T         0 OTHER
ORIGIN      ?
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAGC GCGCCTCGTT CATTCACGTT TTTGAACCCG TGGAGGACGG
      661 GCAGACTCGC GGTGCAAATG TGTTTTACAG CGTGATGGAG CAGATGAAGA TGCTCGACAC
      721 GCTGCAGAAC ACGCAGCTAG ATTAACCCTA GAAAGATAAT CATATTGTGA CGTACGTTAA
      781 AGATAATCAT GCGTAAAATT GACGCATGTG TTTTATCGGT CTGTATATCG AGGTTTATTT
      841 ATTAATTTGA ATAGATATTA AGTTTTATTA TATTTACACT TACATACTAA TAATAAATTC
      901 AACAAACAAT TTATTTATGT TTATTTATTT ATTAAAAAAA AACAAAAACT CAAAATTTCT
      961 TCTATAAAGT AACAAAACTT TTATCGAATT CCTGCAGCCC GGGGGATCCA CTAGTTCTAG
     1021 TGTTCCCACA ATGGTTAATT CGAGCTCGCC CGGGGATCTA ATTCAATTAG AGACTAATTC
     1081 AATTAGAGCT AATTCAATTA GGATCCAAGC TTATCGATTT CGAACCCTCG ACCGCCGGAG
     1141 TATAAATAGA GGCGCTTCGT CTACGGAGCG ACAATTCAAT TCAAACAAGC AAAGTGAACA
     1201 CGTCGCTAAG CGAAAGCTAA GCAAATAAAC AAGCGCAGCT GAACAAGCTA AACAATCGGG
     1261 GTACCGCTAG AGTCGACGGT ACGATCCACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA
     1321 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
     1381 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
     1441 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTG
     1501 GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
     1561 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
     1621 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
     1681 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT
     1741 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA
     1801 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
     1861 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
     1921 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
     1981 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGCG ACTCTAGATC
     2041 ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC
     2101 CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT
     2161 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
     2221 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAAGCTT ATCGATACGC
     2281 GTACGGCACT AGAGCGGCCG CCACCGCGGT CACGTGTCAC TGGGTCAGTG GGTAATCGCT
     2341 TATCCTCGGA TAAACAATTA TCCTCACGGG TAATCGCTTA TCCGCTCGGG TAATCGCTTA
     2401 TCCTCGGGTA ATCGCTTATC CTTGGGTAAT CGCTTATCCT CGGATAAACA ATTATCCTCA
     2461 CGGGTAATCG CTTATCCGCT CGGGTAATCG CTTATCCTCG GGTAATCGCT TATCCTTGGG
     2521 TAATCGCTTA TCCTCGGATA AACAATTATC CTCACGGGTA ATCGCTTATC CGCTCGGGTA
     2581 ATCGCTTATC CTCGGGTAAT CGCTTATCCT TTCACGTTGG GACTCAGTGA GGAGGACCTG
     2641 AATTCCTGCA GCCCGAGCGG AGACTCTAGC GAGCGCCGGA GTATAAATAG AGGCGCTTCG
     2701 TCTACGGAGC GACAATTCAA TTCAAACAAG CAAAGTGAAC ACGTCGCTAA GCGAAAGCTA
     2761 AGCAAATAAA CAAGCGCAGC TGAACAAGCT AAACAATCTG CAGTAAAGTG CAAGTTAAAG
     2821 TGAATCAATT AAAAGTAACC AGCAACCAAG TAAATCAACT GCAACTACTG AAATCTGCCA
     2881 AGAAGTAATT ATTGAATACA AGAAGAGAAC TCTGAATAGG GAATTGGGAA TTCGTTAACA
     2941 GATCGGGGGA TCAATTCGTT AACAGATCTG CGGCCGCGGC TCGAGACGTC GATCTTTGTG
     3001 AAGGAACCTT ACTTCTGTGG TGTGACATAA TTGGACAAAC TACCTACAGA GATTTAAAGC
     3061 TCTAAGGTAA ATATAAAATT TTTAAGTGTA TAATGTGTTA AACTACTGAT TCTAATTGTT
     3121 TGTGTATTTT AGATTCCAAC CTATGGAACT GATGAATGGG AGCAGTGGTG GAATGCCTTT
     3181 AATGAGGAAA ACCTGTTTTG CTCAGAAGAA ATGCCATCTA GTGATGATGA GGCTACTGCT
     3241 GACTCTCAAC ATTCTACTCC TCCAAAAAAG AAGAGAAAGG TAGAAGACCC CAAGGACTTT
     3301 CCTTCAGAAT TGCTAAGTTT TTTGAGTCAT GCTGTGTTTA GTAATAGAAC TCTTGCTTGC
     3361 TTTGCTATTT ACACCACAAA GGAAAAAGCT GCACTGCTAT ACAAGAAAAT TATGGAAAAA
     3421 TATTTGATGT ATAGTGCCTT GACTAGAGAT CATAATCAGC CATACCACAT TTGTAGAGGT
     3481 TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC
     3541 AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT
     3601 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
     3661 CATCAATGTA TCTTATCATG TCTGGATCGA TATCTATAAC AAGAAAATAT ATATATAATA
     3721 AGTTATCACG TAAGTAGAAC ATGAAATAAC AATATAATTA TCGTATGAGT TAAATCTTAA
     3781 AAGTCACGTA AAAGATAATC ATGCGTCATT TTGACTCACG CGGTCGTTAT AGTTCAAAAT
     3841 CAGTGACACT TACCGCATTG ACAAGCACGC CTCACGGGAG CTCCAAGCGG CGACTGAGAT
     3901 GTCCTAAATG CACAGCGACG GATTCGCGCT ATTTAGAAAG AGAGAGCAAT ATTTCAAGAA
     3961 TGCATGCGTC AATTTTACGC AGACTATCTT TCTAGGGTTA ATCTAGCTGC ATCAGGATCA
     4021 TATCGTCGGG TCTTTTTTCC GGCTCAGTCA TCGCCCAAGC TGGCGCTATC TGGGCATCGG
     4081 GGAGGAAGAA GCCCGTGCCT TTTCCCGCGA GGTTGAAGCG GCATGGAAAG AGTTTGCCGA
     4141 GGATGACTGC TGCTGCATTG ACGTTGAGCG AAAACGCACG TTTACCATGA TGATTCGGGA
     4201 AGGTGTGGCC ATGCACGCCT TTAACGGTGA ACTGTTCGTT CAGGCCACCT GGGATACCAG
     4261 TTCGTCGCGG CTTTTCCGGA CACAGTTCCG GATGGTCAGC CCGAAGCGCA TCAGCAACCC
     4321 GAACAATACC GGCGACAGCC GGAACTGCCG TGCCGGTGTG CAGATTAATG ACAGCGGTGC
     4381 GGCGCTGGGA TATTACGTCA GCGAGGACGG GTATCCTGGC TGGATGCCGC AGAAATGGAC
     4441 ATGGATACCC CGTGAGTTAC CCGGCGGGCG CGCTTGGCGT AATCATGGTC ATAGCTGTTT
     4501 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG
     4561 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG
     4621 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
     4681 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC
     4741 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     4801 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     4861 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
     4921 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     4981 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     5041 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     5101 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     5161 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     5221 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     5281 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT
     5341 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     5401 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     5461 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     5521 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     5581 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     5641 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     5701 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     5761 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA
     5821 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     5881 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     5941 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     6001 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     6061 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     6121 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     6181 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     6241 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     6301 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     6361 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     6421 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     6481 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     6541 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     6601 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCAC
//
