LOCUS       pXL-BACII-              8866 bp ss-DNA     circular SYN 27-Jul-2014
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     Olena Riabinina, Potter Lab, Johns Hopkins University School of
            Medicine
FEATURES             Location/Qualifiers
     misc_feature    3..459
                     /note=f1 origin
     LTR             746..808
                     /note=3'  Terminal Repeat
     misc_feature    1022..2287
                     /note=3xP3-ECFP
     promoter        1059..1099
                     /note=3xP3
     misc_feature    1301..1303
                     /note=ECFP start
     gene            1301..2020
                     /note=ECFP gene
     misc_feature    2018..2020
                     /note=ECFP STOP
     enhancer        2330..2611
                     /note=15xQUAS
     promoter        2655..2928
                     /note=TATA
     gene            2990..3661
                     /note=CD8
     gene            3662..4378
                     /note=GFP
     terminator      4386..5083
                     /note=SV40
     terminator      5174..5870
                     /note=SV40
     LTR             6192..6226
                     /note=5'  Terminal Repeat
     gene            complement(6721..6843)
                     /note=alpha lacZ gene
     rep_origin      complement(6937..7877)
                     /note=ColE1 ori
     gene            complement(7880..8737)
                     /note=Amp resistance gene
BASE COUNT      2498 A      2089 C      2038 G      2241 T         0 OTHER
ORIGIN      ?
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAGC GCGCCTCGTT CATTCACGTT TTTGAACCCG TGGAGGACGG
      661 GCAGACTCGC GGTGCAAATG TGTTTTACAG CGTGATGGAG CAGATGAAGA TGCTCGACAC
      721 GCTGCAGAAC ACGCAGCTAG ATTAACCCTA GAAAGATAAT CATATTGTGA CGTACGTTAA
      781 AGATAATCAT GCGTAAAATT GACGCATGTG TTTTATCGGT CTGTATATCG AGGTTTATTT
      841 ATTAATTTGA ATAGATATTA AGTTTTATTA TATTTACACT TACATACTAA TAATAAATTC
      901 AACAAACAAT TTATTTATGT TTATTTATTT ATTAAAAAAA AACAAAAACT CAAAATTTCT
      961 TCTATAAAGT AACAAAACTT TTATCGAATT CCTGCAGCCC GGGGGATCCA CTAGTTCTAG
     1021 TGTTCCCACA ATGGTTAATT CGAGCTCGCC CGGGGATCTA ATTCAATTAG AGACTAATTC
     1081 AATTAGAGCT AATTCAATTA GGATCCAAGC TTATCGATTT CGAACCCTCG ACCGCCGGAG
     1141 TATAAATAGA GGCGCTTCGT CTACGGAGCG ACAATTCAAT TCAAACAAGC AAAGTGAACA
     1201 CGTCGCTAAG CGAAAGCTAA GCAAATAAAC AAGCGCAGCT GAACAAGCTA AACAATCGGG
     1261 GTACCGCTAG AGTCGACGGT ACGATCCACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA
     1321 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
     1381 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
     1441 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTG
     1501 GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
     1561 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
     1621 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
     1681 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT
     1741 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA
     1801 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
     1861 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
     1921 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
     1981 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGCG ACTCTAGATC
     2041 ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC
     2101 CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT
     2161 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
     2221 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAAGCTT ATCGATACGC
     2281 GTACGGCACT AGAGCGGCCG CCACCGCGGT CACGTGTCAC TGGGTCAGTG GGTAATCGCT
     2341 TATCCTCGGA TAAACAATTA TCCTCACGGG TAATCGCTTA TCCGCTCGGG TAATCGCTTA
     2401 TCCTCGGGTA ATCGCTTATC CTTGGGTAAT CGCTTATCCT CGGATAAACA ATTATCCTCA
     2461 CGGGTAATCG CTTATCCGCT CGGGTAATCG CTTATCCTCG GGTAATCGCT TATCCTTGGG
     2521 TAATCGCTTA TCCTCGGATA AACAATTATC CTCACGGGTA ATCGCTTATC CGCTCGGGTA
     2581 ATCGCTTATC CTCGGGTAAT CGCTTATCCT TTCACGTTGG GACTCAGTGA GGAGGACCTG
     2641 AATTCCTGCA GCCCGAGCGG AGACTCTAGC GAGCGCCGGA GTATAAATAG AGGCGCTTCG
     2701 TCTACGGAGC GACAATTCAA TTCAAACAAG CAAAGTGAAC ACGTCGCTAA GCGAAAGCTA
     2761 AGCAAATAAA CAAGCGCAGC TGAACAAGCT AAACAATCTG CAGTAAAGTG CAAGTTAAAG
     2821 TGAATCAATT AAAAGTAACC AGCAACCAAG TAAATCAACT GCAACTACTG AAATCTGCCA
     2881 AGAAGTAATT ATTGAATACA AGAAGAGAAC TCTGAATAGG GAATTGGGAA TTCGTTAACA
     2941 GATCGGGGGA TCAATTCGTT AACAGATCTG CGGCCGCGGC TCGAGCAAAA TGGCCTCACC
     3001 GTTGACCCGC TTTCTGTCGC TGAACCTGCT GCTGCTGGGT GAGTCGATTA TCCTGGGGAG
     3061 TGGAGAAGCT AAGCCACAGG CACCCGAACT CCGAATCTTT CCAAAGAAAA TGGACGCCGA
     3121 ACTTGGTCAG AAGGTGGACC TGGTATGTGA AGTGTTGGGG TCCGTTTCGC AAGGATGCTC
     3181 TTGGCTCTTC CAGAACTCCA GCTCCAAACT CCCCCAGCCC ACCTTCGTTG TCTATATGGC
     3241 TTCATCCCAC AACAAGATAA CGTGGGACGA GAAGCTGAAT TCGTCGAAAC TGTTTTCTGC
     3301 CATGAGGGAC ACGAATAATA AGTACGTTCT CACCCTGAAC AAGTTCAGCA AGGAAAACGA
     3361 AGGCTACTAT TTCTGCTCAG TCATCAGCAA CTCGGTGATG TACTTCAGTT CTGTCGTGCC
     3421 AGTCCTTCAG AAAGTGAACT CTACTACTAC CAAGCCAGTG CTGCGAACTC CCTCACCTGT
     3481 GCACCCTACC GGGACATCTC AGCCCCAGAG ACCAGAAGAT TGTCGGCCCC GTGGCTCAGT
     3541 GAAGGGGACC GGATTGGACT TCGCCTGTGA TATTTACATC TGGGCACCCT TGGCCGGAAT
     3601 CTGCGTGGCC CTTCTGCTGT CCTTGATCAT CACTCTCATC TGCTACCACA GCCGCGGATC
     3661 CATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG
     3721 TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG
     3781 AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGCCAACACT
     3841 TGTCACTACT TTAACTTATG GTGTTCAATG CTTTTCAAGA TACCCAGATC ATATGAAACA
     3901 GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTC CAGGAAAGAA CTATATTTTT
     3961 CAAAGATGAC GGGAACTACA AGACACGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT
     4021 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA
     4081 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG
     4141 AATCAAAGTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA
     4201 CCATTATCAA CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA
     4261 CCTGTCCACA CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT
     4321 TCTTGAGTTT GTAACAGCTG CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAATC
     4381 TAGAGGATCT TTGTGAAGGA ACCTTACTTC TGTGGTGTGA CATAATTGGA CAAACTACCT
     4441 ACAGAGATTT AAAGCTCTAA GGTAAATATA AAATTTTTAA GTGTATAATG TGTTAAACTA
     4501 CTGATTCTAA TTGTTTGTGT ATTTTAGATT CCAACCTATG GAACTGATGA ATGGGAGCAG
     4561 TGGTGGAATG CCTTTAATGA GGAAAACCTG TTTTGCTCAG AAGAAATGCC ATCTAGTGAT
     4621 GATGAGGCTA CTGCTGACTC TCAACATTCT ACTCCTCCAA AAAAGAAGAG AAAGGTAGAA
     4681 GACCCCAAGG ACTTTCCTTC AGAATTGCTA AGTTTTTTGA GTCATGCTGT GTTTAGTAAT
     4741 AGAACTCTTG CTTGCTTTGC TATTTACACC ACAAAGGAAA AAGCTGCACT GCTATACAAG
     4801 AAAATTATGG AAAAATATTT GATGTATAGT GCCTTGACTA GAGATCATAA TCAGCCATAC
     4861 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA
     4921 ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA
     4981 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
     5041 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCACTAGA AGGCCTTAGT
     5101 ATGGGGTACC CCAAGGGCGA ATTCTGCAGA TATCCATCAC ACTGGCGGCC GCGGCTCGAG
     5161 GGTACCTCTA GAGGATCTTT GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA
     5221 AACTACCTAC AGAGATTTAA AGCTCTAAGG TAAATATAAA ATTTTTAAGT GTATAATGTG
     5281 TTAAACTACT GATTCTAATT GTTTGTGTAT TTTAGATTCC AACCTATGGA ACTGATGAAT
     5341 GGGAGCAGTG GTGGAATGCC TTTAATGAGG AAAACCTGTT TTGCTCAGAA GAAATGCCAT
     5401 CTAGTGATGA TGAGGCTACT GCTGACTCTC AACATTCTAC TCCTCCAAAA AAGAAGAGAA
     5461 AGGTAGAAGA CCCCAAGGAC TTTCCTTCAG AATTGCTAAG TTTTTTGAGT CATGCTGTGT
     5521 TTAGTAATAG AACTCTTGCT TGCTTTGCTA TTTACACCAC AAAGGAAAAA GCTGCACTGC
     5581 TATACAAGAA AATTATGGAA AAATATTTGA TGTATAGTGC CTTGACTAGA GATCATAATC
     5641 AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG
     5701 AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT
     5761 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT
     5821 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CCACTAGAAG
     5881 GCCTAGATCT CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCTATAACA AGAAAATATA
     5941 TATATAATAA GTTATCACGT AAGTAGAACA TGAAATAACA ATATAATTAT CGTATGAGTT
     6001 AAATCTTAAA AGTCACGTAA AAGATAATCA TGCGTCATTT TGACTCACGC GGTCGTTATA
     6061 GTTCAAAATC AGTGACACTT ACCGCATTGA CAAGCACGCC TCACGGGAGC TCCAAGCGGC
     6121 GACTGAGATG TCCTAAATGC ACAGCGACGG ATTCGCGCTA TTTAGAAAGA GAGAGCAATA
     6181 TTTCAAGAAT GCATGCGTCA ATTTTACGCA GACTATCTTT CTAGGGTTAA TCTAGCTGCA
     6241 TCAGGATCAT ATCGTCGGGT CTTTTTTCCG GCTCAGTCAT CGCCCAAGCT GGCGCTATCT
     6301 GGGCATCGGG GAGGAAGAAG CCCGTGCCTT TTCCCGCGAG GTTGAAGCGG CATGGAAAGA
     6361 GTTTGCCGAG GATGACTGCT GCTGCATTGA CGTTGAGCGA AAACGCACGT TTACCATGAT
     6421 GATTCGGGAA GGTGTGGCCA TGCACGCCTT TAACGGTGAA CTGTTCGTTC AGGCCACCTG
     6481 GGATACCAGT TCGTCGCGGC TTTTCCGGAC ACAGTTCCGG ATGGTCAGCC CGAAGCGCAT
     6541 CAGCAACCCG AACAATACCG GCGACAGCCG GAACTGCCGT GCCGGTGTGC AGATTAATGA
     6601 CAGCGGTGCG GCGCTGGGAT ATTACGTCAG CGAGGACGGG TATCCTGGCT GGATGCCGCA
     6661 GAAATGGACA TGGATACCCC GTGAGTTACC CGGCGGGCGC GCTTGGCGTA ATCATGGTCA
     6721 TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA
     6781 AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG
     6841 CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC
     6901 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC
     6961 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA
     7021 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA
     7081 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT
     7141 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
     7201 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
     7261 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA
     7321 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA
     7381 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
     7441 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
     7501 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG
     7561 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC
     7621 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG
     7681 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     7741 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     7801 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     7861 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     7921 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     7981 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA
     8041 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     8101 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     8161 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     8221 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     8281 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     8341 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     8401 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     8461 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     8521 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     8581 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     8641 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     8701 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     8761 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     8821 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCAC
//
