LOCUS BB3_pGAP_BbsI(intern)_RPS25Att_pTEF_Cas9_cycTT_G418 8515 bp DNA circular UNA FEATURES Location/Qualifiers Vector join(6643..8515,1..730) /origin="BB3_pTEF_KanR_TEFt_AC (2,633bp circular)Target vector: from BsaI cut at 760^761 to BsaI cut at 730^731, 2,603bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" rep_origin complement(13..686) /label="pUC_ORI_aus_pGAPZB" primer 448..467 /label="JH_77_fw" primer 524..541 /label="B1_13" terminator 691..730 /label="BBa_B1007" FS 731..734 /label="FS A" Vector join(1751..>1754,<731..734) /origin="BB2_L_AB_syn_BbsI_6 (2,215bp circular)Target vector: from BpiI cut at 796^797 to BpiI cut at 764^765, 2,183bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" Restriction_sit 734^735 /note="Name: BbsI" /note="Pattern: gaagac" /note="Number_of_matches: 2" /note="cut_0_on_positive_strand: 2153^2154" /note="cut_0_on_negative_strand: 2157^2158" /note="inhibited_by: " /note="site_type: other" /note="restriction site" /label="BbsI" promoter 737..>1229 /label="pGAP" Restriction_sit join(1229^1230,complement(1229^1230)) /note="Name: BbsI" /note="Pattern: gaagac" /note="Number_of_matches: 2" /note="cut_0_on_positive_strand: 2648^2649" /note="cut_0_on_negative_strand: 2652^2653" /note="inhibited_by: " /note="site_type: other" /note="restriction site" /label="BbsI" primer <1230..>1261 /label="L_FS23_BbsI_fw" Linker 1230..1265 /label="FS 23 Linker" FS 1230..1233 /label="FS2" primer complement(<1234..>1265) /label="L_FS23_BbsI_rev" misc_feature join(<1262..1294,complement(1295..1727),complement(1728..>1754)) /feature_type="PCR fragment" /note="Forward primer name: RPS25ATT_rv_fs4" /note="Forward primer: gAtcgGtctCcAGCgGTCAAATATTCAGATGGTGATGC" /note="Forward primer misprimes: 9" /note="Reverse primer name: RPS25ATT_FW_fs3" /note="Reverse primer: gaCaggtctcCgcttTTAGTGTACATCTGATAATATAGTACTAC" /note="Reverse primer misprimes: 13" /note="Fragment length: 515" /label="Fragment (TT_BB1_ RPS25Att (RPS25ATT_rv_fs4 - RPS25ATT_FW_fs3))" terminator 1262..1750 /origin="Fragment (TT_BB1_ RPS25Att (RPS25ATT_rv_fs4 - RPS25ATT_FW_fs3)) (515bp linear)Fragment: from BsaI cut at 11^12 to BsaI cut at 500^501, 489bpOrientation: reverse complementedPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" /label="RPS25Att" FS 1751..1754 /label="FS 4" misc_feature join(<6639..>6642,<1755..>1758) /feature_type="Insertion" /origin="BsaI_B_1_4_C_FW (118bp linear)Fragment: from SpeI cut at 5^6 to AgeI cut at 109^110, 104bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" /label="Insert" Vector join(6639..>6642,<1755..1758) /origin="PCRproductNewBB2_BsaI_B_1_4_C_FW (2.215bp circular)Target vector: from BbsI cut at 2199^2200 to BbsI cut at 2167^2168, 2.183bpOrientation: originalPlus strand 5': no changePlus strand 3': no changeMinus strand 5': no changeMinus strand 3': no change" promoter 1763..2175 /label="TEF1p" primer <2176..2200 /label="FS2_Cas9_BsaI_fw" CDS 2177..6316 /label="Cas9" Taming 2227 /label="C>T" primer complement(3588..3607) /label="1_cas9_rev" primer 3588..3607 /label="1_cas9_fw" Taming 3592 /label="C>t" primer complement(3862..3883) /label="2_cas9_rev" primer 3862..3883 /label="2_cas9_fw" Taming 3874 /label="A>g" primer complement(4331..4348) /label="3_cas9_rev" primer 4331..4348 /label="3_cas9_fw" Taming 4333 /label="T>c" primer complement(5046..5067) /label="4_cas9_rev" primer 5046..5067 /label="4_cas9_fw" Taming 5062 /label="G>c" primer complement(5913..5930) /label="5_cas9_rev" primer 5913..5931 /label="5_cas9_fw" Taming 5920 /label="T>c" NLS 6281..6316 /label="NLS SV40" primer complement(6294..>6320) /label="FS3_Pol7_Cas9_BsaI_rev" terminator <6317..>6638 /label="FS3_CYCtt_FS4" primer complement(6443..6462) /label="Seq cycTT Rev1" CDS 6651..6994 /label="TEFpromoter" mutation 6824 /label="C to G" primer 6853..6870 /label="Seq_MX_FW" primer complement(6878..6897) /label="JH_78_rev" CDS 6995..7804 /label="KanR" CDS 7813..8011 /label="TEFterminator" primer complement(7836..7857) /label="Seq_MX_REV" misc_feature 8012..8515 /label="CEN6_ARS4" mutation 8233 /label="G/A, actually G/C" ORIGIN 1 AGCGATCGCG CTGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 61 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 121 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 181 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 241 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 301 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 361 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 421 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 481 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 541 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 601 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 661 AGATCCTTTG ATCTTTTCTA CGGGGTGGAG cgcaaaaaac cccgcccctg acagggcggg 721 gttttttcgc GATCGGAGGA TCCTTTTTTG TAGAAATGTC TTGGTGTCCT CGTCCAATCA 781 GGTAGCCATC TCTGAAATAT CTGGCTCCGT TGCAACTCCG AACGACCTGC TGGCAACGTA 841 AAATTCTCCG GGGTAAAACT TAAATGTGGA GTAATGGAAC CAGAAACGTC TCTTCCCTTC 901 TCTCTCCTTC CACCGCCCGT TACCGTCCCT AGGAAATTTT ACTCTGCTGG AGAGCTTCTT 961 CTACGGCCCC CTTGCAGCAA TGCTCTTCCC AGCATTACGT TGCGGGTAAA ACGGAGGTCG 1021 TGTACCCGAC CTAGCAGCCC AGGGATGGAA AAGTCCCGGC CGTCGCTGGC AATAATAGCG 1081 GGCGGACGCA TGTCATGAGA TTATTGGAAA CCACCAGAAT CGAATATAAA AGGCGAACAC 1141 CTTTCCCAAT TTTGGTTTCT CCTGACCCAA AGACTTTAAA TTTAATTTAT TTGTCCCTAT 1201 TTCAATCAAT TGAACAACTA TCAAAACACC ATGGCGTCTT CGGATCCCTC GAGGAAGACG 1261 CGCTTTTAGT GTACATCTGA TAATATAGTA CTACCACGTA TGATAATGTA GAGAATAGGC 1321 TTCCTTGTCG AGTGTGTTTG CAGTTTTCTT GAGTTTCAAG GTTTAAATGC TGGTATATTA 1381 GTTCATCGAA GGTTTCAGCC AATAGCACCT TAAATCAATC AAACTAATTC GACTCTTACG 1441 AAAGAGCCTA CTGTGTTTAG TATCGAAGTC GTTTACCTTT CATGTTGAAT AGCTTCCTCT 1501 CTGACCCTAA CATTTCAAGA TCCTCCTAAA GTTACCCGGA TTGTGAAATT CTAATGATCC 1561 ACCTGCCCAA TGCATTTTTT CTTTATTCAG TTTACCTTTT TTACCTAATA TACGAGCTTG 1621 TTAAAGTAAG TGGCACTGCA ATACTAGGCT TATTGTTGAT ATTATGATGA ATCGTTTTCA 1681 CAAACTTGAT TTCCTGTGAA CTCACCATGT ACTAAGGAAA AAAACATGCA TCACCATCTG 1741 AATATTTGAC CGCTCCGGGG AGCACACACC ATAGCTTCAA AATGTTTCTA CTCCTTTTTT 1801 ACTCTTCCAG ATTTTCTCGG ACTCCGCGCA TCGCCGTACC ACTTCAAAAC ACCCAAGCAC 1861 AGCATACTAA ATTTTCCCTC TTTCTTCCTC TAGGGTGTCG TTAATTACCC GTACTAAAGG 1921 TTTGGAAAAG AAAAAAGcGA CCGCCTCGTT TCTTTTTCTT CGTCGAAAAA GGCAATAAAA 1981 ATTTTTATCA CGTTTCTTTT TCTTGAAAAT TTTTTTTTTT GATTTTTTTC TCTTTCGATG 2041 ACCTCCCATT GATATTTAAG TTAATAAACG GTgTTCAATT TCTCAAGTTT CAGTTTCATT 2101 TTTCTTGTTC TATTACAACT TTTTTTACTT CTTGTTCATT AGAAAGAAAG CATAGCAATC 2161 TAATCTAAGG GCGGTCATGG ACAAGAAGTA CTCCATTGGG CTCGATATCG GCACAAACAG 2221 CGTCGGTTGG GCCGTCATTA CGGACGAGTA CAAGGTGCCG AGCAAAAAAT TCAAAGTTCT 2281 GGGCAATACC GATCGCCACA GCATAAAGAA GAACCTCATT GGCGCCCTCC TGTTCGACTC 2341 CGGGGAGACG GCCGAAGCCA CGCGGCTCAA AAGAACAGCA CGGCGCAGAT ATACCCGCAG 2401 AAAGAATCGG ATCTGCTACC TGCAGGAGAT CTTTAGTAAT GAGATGGCTA AGGTGGATGA 2461 CTCTTTCTTC CATAGGCTGG AGGAGTCCTT TTTGGTGGAG GAGGATAAAA AGCACGAGCG 2521 CCACCCAATC TTTGGCAATA TCGTGGACGA GGTGGCGTAC CATGAAAAGT ACCCAACCAT 2581 ATATCATCTG AGGAAGAAGC TTGTAGACAG TACTGATAAG GCTGACTTGC GGTTGATCTA 2641 TCTCGCGCTG GCGCATATGA TCAAATTTCG GGGACACTTC CTCATCGAGG GGGACCTGAA 2701 CCCAGACAAC AGCGATGTCG ACAAACTCTT TATCCAACTG GTTCAGACTT ACAATCAGCT 2761 TTTCGAAGAG AACCCGATCA ACGCATCCGG AGTTGACGCC AAAGCAATCC TGAGCGCTAG 2821 GCTGTCCAAA TCCCGGCGGC TCGAAAACCT CATCGCACAG CTCCCTGGGG AGAAGAAGAA 2881 CGGCCTGTTT GGTAATCTTA TCGCCCTGTC ACTCGGGCTG ACCCCCAACT TTAAATCTAA 2941 CTTCGACCTG GCCGAAGATG CCAAGCTTCA ACTGAGCAAA GACACCTACG ATGATGATCT 3001 CGACAATCTG CTGGCCCAGA TCGGCGACCA GTACGCAGAC CTTTTTTTGG CGGCAAAGAA 3061 CCTGTCAGAC GCCATTCTGC TGAGTGATAT TCTGCGAGTG AACACGGAGA TCACCAAAGC 3121 TCCGCTGAGC GCTAGTATGA TCAAGCGCTA TGATGAGCAC CACCAAGACT TGACTTTGCT 3181 GAAGGCCCTT GTCAGACAGC AACTGCCTGA GAAGTACAAG GAAATTTTCT TCGATCAGTC 3241 TAAAAATGGC TACGCCGGAT ACATTGACGG CGGAGCAAGC CAGGAGGAAT TTTACAAATT 3301 TATTAAGCCC ATCTTGGAAA AAATGGACGG CACCGAGGAG CTGCTGGTAA AGCTTAACAG 3361 AGAAGATCTG TTGCGCAAAC AGCGCACTTT CGACAATGGA AGCATCCCCC ACCAGATTCA 3421 CCTGGGCGAA CTGCACGCTA TCCTCAGGCG GCAAGAGGAT TTCTACCCCT TTTTGAAAGA 3481 TAACAGGGAA AAGATTGAGA AAATCCTCAC ATTTCGGATA CCCTACTATG TAGGCCCCCT 3541 CGCCCGGGGA AATTCCAGAT TCGCGTGGAT GACTCGCAAA TCAGAAGAGA CtATCACTCC 3601 CTGGAACTTC GAGGAAGTCG TGGATAAGGG GGCCTCTGCC CAGTCCTTCA TCGAAAGGAT 3661 GACTAACTTT GATAAAAATC TGCCTAACGA AAAGGTGCTT CCTAAACACT CTCTGCTGTA 3721 CGAGTACTTC ACAGTTTATA ACGAGCTCAC CAAGGTCAAA TACGTCACAG AAGGGATGAG 3781 AAAGCCAGCA TTCCTGTCTG GAGAGCAGAA GAAAGCTATC GTGGACCTCC TCTTCAAGAC 3841 GAACCGGAAA GTTACCGTGA AACAGCTCAA AGAgGACTAT TTCAAAAAGA TTGAATGTTT 3901 CGACTCTGTT GAAATCAGCG GAGTGGAGGA TCGCTTCAAC GCATCCCTGG GAACGTATCA 3961 CGATCTCCTG AAAATCATTA AAGACAAGGA CTTCCTGGAC AATGAGGAGA ACGAGGACAT 4021 TCTTGAGGAC ATTGTCCTCA CCCTTACGTT GTTTGAAGAT AGGGAGATGA TTGAAGAACG 4081 CTTGAAAACT TACGCTCATC TCTTCGACGA CAAAGTCATG AAACAGCTCA AGAGGCGCCG 4141 ATATACAGGA TGGGGGCGGC TGTCAAGAAA ACTGATCAAT GGGATCCGAG ACAAGCAGAG 4201 TGGAAAGACA ATCCTGGATT TTCTTAAGTC CGATGGATTT GCCAACCGGA ACTTCATGCA 4261 GTTGATCCAT GATGACTCTC TCACCTTTAA GGAGGACATC CAGAAAGCAC AAGTTTCTGG 4321 CCAGGGGGAC AGcCTTCACG AGCACATCGC TAATCTTGCA GGTAGCCCAG CTATCAAAAA 4381 GGGAATACTG CAGACCGTTA AGGTCGTGGA TGAACTCGTC AAAGTAATGG GAAGGCATAA 4441 GCCCGAGAAT ATCGTTATCG AGATGGCCCG AGAGAACCAA ACTACCCAGA AGGGACAGAA 4501 GAACAGTAGG GAAAGGATGA AGAGGATTGA AGAGGGTATA AAAGAACTGG GGTCCCAAAT 4561 CCTTAAGGAA CACCCAGTTG AAAACACCCA GCTTCAGAAT GAGAAGCTCT ACCTGTACTA 4621 CCTGCAGAAC GGCAGGGACA TGTACGTGGA TCAGGAACTG GACATCAATC GGCTCTCCGA 4681 CTACGACGTG GATCATATCG TGCCCCAGTC TTTTCTCAAA GATGATTCTA TTGATAATAA 4741 AGTGTTGACA AGATCCGATA AAAATAGAGG GAAGAGTGAT AACGTCCCCT CAGAAGAAGT 4801 TGTCAAGAAA ATGAAAAATT ATTGGCGGCA GCTGCTGAAC GCCAAACTGA TCACACAACG 4861 GAAGTTCGAT AATCTGACTA AGGCTGAACG AGGTGGCCTG TCTGAGTTGG ATAAAGCCGG 4921 CTTCATCAAA AGGCAGCTTG TTGAGACACG CCAGATCACC AAGCACGTGG CCCAAATTCT 4981 CGATTCACGC ATGAACACCA AGTACGATGA AAATGACAAA CTGATTCGAG AGGTGAAAGT 5041 TATTACTCTG AAGTCTAAGC TcGTCTCAGA TTTCAGAAAG GACTTTCAGT TTTATAAGGT 5101 GAGAGAGATC AACAATTACC ACCATGCGCA TGATGCCTAC CTGAATGCAG TGGTAGGCAC 5161 TGCACTTATC AAAAAATATC CCAAGCTTGA ATCTGAATTT GTTTACGGAG ACTATAAAGT 5221 GTACGATGTT AGGAAAATGA TCGCAAAGTC TGAGCAGGAA ATAGGCAAGG CCACCGCTAA 5281 GTACTTCTTT TACAGCAATA TTATGAATTT TTTCAAGACC GAGATTACAC TGGCCAATGG 5341 AGAGATTCGG AAGCGACCAC TTATCGAAAC AAACGGAGAA ACAGGAGAAA TCGTGTGGGA 5401 CAAGGGTAGG GATTTCGCGA CAGTCCGGAA GGTCCTGTCC ATGCCGCAGG TGAACATCGT 5461 TAAAAAGACC GAAGTACAGA CCGGAGGCTT CTCCAAGGAA AGTATCCTCC CGAAAAGGAA 5521 CAGCGACAAG CTGATCGCAC GCAAAAAAGA TTGGGACCCC AAGAAATACG GCGGATTCGA 5581 TTCTCCTACA GTCGCTTACA GTGTACTGGT TGTGGCCAAA GTGGAGAAAG GGAAGTCTAA 5641 AAAACTCAAA AGCGTCAAGG AACTGCTGGG CATCACAATC ATGGAGCGAT CAAGCTTCGA 5701 AAAAAACCCC ATCGACTTTC TCGAGGCGAA AGGATATAAA GAGGTCAAAA AAGACCTCAT 5761 CATTAAGCTT CCCAAGTACT CTCTCTTTGA GCTTGAAAAC GGCCGGAAAC GAATGCTCGC 5821 TAGTGCGGGC GAGCTGCAGA AAGGTAACGA GCTGGCACTG CCCTCTAAAT ACGTTAATTT 5881 CTTGTATCTG GCCAGCCACT ATGAAAAGCT CAAAGGGTCc CCCGAAGATA ATGAGCAGAA 5941 GCAGCTGTTC GTGGAACAAC ACAAACACTA CCTTGATGAG ATCATCGAGC AAATAAGCGA 6001 ATTCTCCAAA AGAGTGATCC TCGCCGACGC TAACCTCGAT AAGGTGCTTT CTGCTTACAA 6061 TAAGCACAGG GATAAGCCCA TCAGGGAGCA GGCAGAAAAC ATTATCCACT TGTTTACTCT 6121 GACCAACTTG GGCGCGCCTG CAGCCTTCAA GTACTTCGAC ACCACCATAG ACAGAAAGCG 6181 GTACACCTCT ACAAAGGAGG TCCTGGACGC CACACTGATT CATCAGTCAA TTACGGGGCT 6241 CTATGAAACA AGAATCGACC TCTCTCAGCT CGGTGGAGAC AGCAGGGCTG ACCCCAAGAA 6301 GAAGAGGAAG GTGTGAGCTT CGTCCGACGG CGGCCCACGG GTCCCAGGCC TCGGAGATCC 6361 GTCCCCCTTT TCCTTTGTCG ATATCATGTA ATTAGTTATG TCACGCTTAC ATTCACGCCC 6421 TCCCCCCACA TCCGCTCTAA CCGAAAAGGA AGGAGTTAGA CAACCTGAAG TCTAGGTCCC 6481 TATTTATTTT TTTATAGTTA TGTTAGTATT AAGAACGTTA TTTATATTTC AAATTTTTCT 6541 TTTTTTTCTG TACAGACGCG TGTACGCATG TAACATTATA CTGAAAACCT TGCTTGAGAA 6601 GGTTTTGGGA CGCTCGAAGG CTTTAATTTG CAAGCTGGCG CTAATTCATG GACATGGAGG 6661 CCCAGAATAC CCTCCTTGAC AGTCTTGACG TGCGCAGCTC AGGGGCATGA TGTGACTGTC 6721 GCCCGTACAT TTAGCCCATA CATCCCCATG TATAATCATT TGCATCCATA CATTTTGATG 6781 GCCGCACGGC GCGAAGCAAA AATTACGGCT CCTCGCTGCA GACGTGCGAG CAGGGAAACG 6841 CTCCCCTCAC AGACGCGTTG AATTGTCCCC ACGCCGCGCC CCTGTAGAGA AATATAAAAG 6901 GTTAGGATTT GCCACTGAGG TTCTTCTTTC ATATACTTCC TTTTAAAATC TTGCTAGGAT 6961 ACAGTTCTCA CATCACATCC GAACATAAAC AACCATGGGT AAGGAAAAGA CTCACGTTTC 7021 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA 7081 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGATTGTAT GGGAAGCCCG ATGCGCCAGA 7141 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG 7201 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC 7261 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGCAAA ACAGCATTCC AGGTATTAGA 7321 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT 7381 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA 7441 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA 7501 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAG CTTTTGCCAT TCTCACCGGA 7561 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT 7621 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT 7681 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA 7741 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT 7801 CTAATCAGTA CTGACAATAA AAAGATTCTT GTTTTCAAGA ACTTGTCATT TGTATAGTTT 7861 TTTTATATTG TAGTTGTTCT ATTTTAATCA AATGTTAGCG TGATTTATAT TTTTTTTCGC 7921 CTCGACATCA TCTGCCCAGA TGCGAAGTTA AGTGCGCAGA AAGTAATATC ATGCGTCAAT 7981 CGTATGTGAA TGCTGGTCGC TATACTGGCT TCCTTTTCAT CACGTGCTAT AAAAATAATT 8041 ATAATTTAAA TTTTTTAATA TAAATATATA AATTAAAAAT AGAAAGTAAA AAAAGAAATT 8101 AAAGAAAAAA TAGTTTTTGT TTTCCGAAGA TGTAAAAGAC TCTAGGGGGA TCGCCAACAA 8161 ATACTACCTT TTATCTTGCT CTTCCTGCTC TCAGGTATTA ATGCCGAATT GTTTCATCTT 8221 GTCTGTGTAG AACACCACAC ACGAAAATCC TGTGATTTTA CATTTTACTT ATCGTTAATC 8281 GAATGTATAT CTATTTAATC TGCTTTTCTT GTCTAATAAA TATATATGTA AAGTACGCTT 8341 TTTGTTGAAA TTTTTTAAAC CTTTGTTTAT TTTTTTTTCT TCATTCCGTA ACTCTTCTAC 8401 CTTCTTTATT TACTTTCTAA AATCCAAATA CAAAACATAA AAATAAATAA ACACAGAGTA 8461 AATTCCCAAA TTATTCCATC ATTAAAAGAT ACGAGGCGCG TGTAAGTTAC AGGCA //