LOCUS       Expression\Clone/pAFMW/pENTR-D-AthHen1dmeCO        8796 bp    DNA     circular     22-APR-2015
DEFINITION  attB1-attB2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|731855507|
COMMENT     VNTDBDATE|731855507|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Expression Clone/pAFMW/pENTR-D-AthHen1dmeCO|
COMMENT     VNTAUTHORNAME|Sebastian Ruf|
FEATURES             Location/Qualifiers
     misc_feature    5897..5917
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(8789..13)
                     /vntifkey="21"
                     /label=attB2
     CDS             977..1837
                     /vntifkey="4"
                     /label=ampR
     polyA_signal    38..880
                     /vntifkey="25"
                     /label=SV40\sti/polyA
     CDS             5542..5883
                     /vntifkey="4"
                     /label=3xFLAG-6xMyc
     promoter        2868..5440
                     /vntifkey="29"
                     /label=Actin5C
     CDS             5941..8769
                     /vntifkey="4"
                     /label=AthHen1dmeCO
     protein_bind    5937..5940
                     /invitrogen="2410000"
                     /vntifkey="31"
                     /label=directional\TOPO\overhang
     protein_bind    complement(8770..8774)
                     /invitrogen="2460000"
                     /vntifkey="31"
                     /label=TOPO\binding\site
     protein_bind    5932..5936
                     /invitrogen="2470000"
                     /vntifkey="31"
                     /label=TOPO\binding\site
BASE COUNT     2387 a      2016 c      2204 g      2189 t 
ORIGIN
        1 ttgtacaaag tggtgacgta agctagcagg atctttgtga aggaacctta cttctgtggt 
       61 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt 
      121 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc 
      181 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc 
      241 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct 
      301 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt 
      361 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag 
      421 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt 
      481 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg 
      541 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg 
      601 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac 
      661 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 
      721 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 
      781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
      841 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ccgtttaaac tacgcgtaat 
      901 tcaaacaggg ttctggcgtc gttctcgtac tgttttcccc aggccagtgc tttagcgtta 
      961 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     1021 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     1081 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     1141 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     1201 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     1261 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     1321 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     1381 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     1441 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     1501 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     1561 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     1621 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     1681 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     1741 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     1801 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     1861 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     1921 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     1981 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     2041 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     2101 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     2161 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     2221 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     2281 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     2341 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     2401 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     2461 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     2521 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     2581 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     2641 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     2701 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 
     2761 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 
     2821 tcattaatgc aggcaactcg tgaaaggtag gcggatcagc ggccgcagca tgcaattcta 
     2881 tattctaaaa acacaaatga tacttctaaa aaaaaatcat gaatggcatc aactctgaat 
     2941 caaatctttg cagatgcacc tacttctcat ttccactgtc acatcatttt tccagatctc 
     3001 gctgcctgtt atgtggccca caaaccaaga cacgttttat ggccattaaa gctggctgat 
     3061 cgtcgccaaa caccaaatac ataatgaata tgtacacatt cgagaaagaa gcgatcaaag 
     3121 aagcgtcttc gggcggagta ggagaatgcg gaggagaagg agaacgagct gatctagtat 
     3181 ctctccacaa tccaatgcca actgaccaac tggccatatt cggagcaatt tgaagccaat 
     3241 ttccatcgcc tggcgatcgc tccattcttg gctatatgtt tttcaccgtt acccggggcc 
     3301 attttcaaag actcgtcggc aagataagat tgtgtcactc gctgtctctc ttcatttgtc 
     3361 gaagaatgct gaggaatttc gcgatgacgt cggcgagtat tttgaagaat gagaataatt 
     3421 tgtatttata cgaaaatcag ttagtggaat tttctacaaa aacatgttat ctatagataa 
     3481 ttttgttgca aaatatgttg actatgacaa agattgtatg tatatacctt taatgtattc 
     3541 tcattttctt atgtatttat aatggcaatg atgatactga tgatatttta agatgatgcc 
     3601 agaccaaaag gcttgaattt ctgcgtcttt tgccgaacgc agtgcatgtg caattgttgt 
     3661 tttttggaat attcaatttt cggactgtcc gctttgattt cagtttcttg gcttattcaa 
     3721 aaagcaaagt aaagccaaaa aagcgagatg gcaataccaa atgcggcaaa acggtagtgg 
     3781 aaggaaaggg gtgcggggca gcggaaggaa gggtggggcg gggcgtggcg gggtctgtgg 
     3841 ctgggcgcga cgtcaccgac gttggagcca ctcctttgac catgtgtgcg tgtgtgtatt 
     3901 attcgtgtct cgccactcgc cggttgtttt tttcttttta tgctgcgctc tctctagcgc 
     3961 catctcgctt acgcatgctc aacgcaccgc atgttgccgt ttccttttat gcgtcatttt 
     4021 ggctcgaaat aggcaattat ttaaacaaag attagtcaac gaaaacgcta aaataaataa 
     4081 gtctacaata tggttactta ttgccatgtg tgtgcagcca acgatagcaa caaaagcaac 
     4141 aacacaggtg gctttccctc tttcactttt tgtttgcaag ccgcgtgcga gcaagacggc 
     4201 acgaccggca aacgcaatta cgctgacaaa gagcagacga agttttggcg aaaaacatca 
     4261 aggcgcctga tacgaatgca tttgcaataa caattgcgat atttaatatt gtttatgaag 
     4321 ctgtttgact tcaaaacaca caaaaaaaaa aataaaacaa attatttgaa agagaattag 
     4381 gaatcggacg cttatcgtta gggtaacaac aagaaatgct tactgagtca cagcctctgg 
     4441 aaaactgccg caagccagag agagagagaa aaagagggag agcagcttag accgcatgtg 
     4501 cttgtgtgtg aggcgtctct ctcttcgtct ctgttgcgca aacgcataga ctgcactgag 
     4561 aaaatcgatt acctattttt tatgaatgaa tatttgcact attactattc aaaactatta 
     4621 agatagcaat cacattcaat agccaaatac tataccacct gagcgatgca acgaaatgat 
     4681 caatttgagc aaaaatgctg catatttagg acggcatcat tatagaaatg cttcttgctg 
     4741 tgtacttttc tctcgtctgg cagctgtttc gccgttattg ttaaaaccgg cttaagttag 
     4801 gtgtgttttc tacgactagt gaatgcccta ctagaagatg tgtgttgcac aaaatgtccc 
     4861 tggaataacc aatttgaagt gcagatagca gtaaacgtaa gctaatatga atattattta 
     4921 actgtaatgt tttaatatcg ctggacatta ctaataaacc cactataaac acatgtacat 
     4981 atgtatgttt tggcatacaa tgagtagttg gggaaaaaat gtgtaaaagc accgtgacca 
     5041 tcacagcata aagataacca gctgaagtat cgaatatgag taacccccaa attgaatcac 
     5101 atgccgcaac tgataggacc catggaagta cactcttcat ggcgatatac aagacacaca 
     5161 caagcacgaa cacccagttg cggaggaaat tctccgtaaa tgaaaaccca atcggcgaac 
     5221 aattcatacc catatatggt aaaagttttg aacgcgactt gagagcggag agcattgcgg 
     5281 ctgataaggt tttagcgcta agcgggcttt ataaaacggg ctgcgggacc agttttcata 
     5341 tcactaccgt ttgagttctt gtgctgtgtg gatactcctc ccgacacaaa gccgctccat 
     5401 cagccagcag tcgtctaatc cagagacccc ggatccagat atcgaggcct gtctagagaa 
     5461 gcttgttcga atctcgagtg cgcgcttccg gaggtataca cctaggcggt accactgcag 
     5521 tgaattcgga gctccgccac catggactac aaagaccatg acggtgatta taaagatcat 
     5581 gacatcgatt acaaggatga cgatgacaag caccggttga gctccgccac catggagcaa 
     5641 aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac 
     5701 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag 
     5761 ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg 
     5821 aatgaaatgg agagcttggg cgacctcacc atggagcaaa agctcatttc tgaagaggac 
     5881 ttgaatcacc ggtatacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 
     5941 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
     6001 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
     6061 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 
     6121 aatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 
     6181 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctcat cgctattacg 
     6241 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 
     6301 ccagtcacga cgttgtaaaa cgacggccag tgcaacgcga tgacgatgga tagcgattca 
     6361 tcgatgagct gacccgatcg ccgccgccgg agggttgcgt ttgagacggg cgacagattc 
     6421 tagaatggcg ggtggtggca agcacacccc gactccaaag gctattatcc atcaaaagtt 
     6481 tggcgctaag gcctcgtata cagtggagga agttcatgac tcgtcgcagt cgggctgcct 
     6541 gggcctggcc attccgcaga agggtccgtg cctctaccgg tgtcacctgc agctgccaga 
     6601 attctccgtg gtgagcaatg tattcaaaaa gaagaaggat agtgagcaga gtgctgctga 
     6661 gctggccttg gataaactgg gtattcgacc ccagaacgac gatttgacag tggatgaggc 
     6721 gcgcgatgag atcgtgggca ggatcaaata tatattcagt gacgagttcc tgagtgccga 
     6781 gcacccgttg ggcgcacact tgcgcgcagc cctgcgtcgc gatggcgaac gttgcggctc 
     6841 ggttccggtt agtgtgattg ctaccgtgga cgccaaaatt aacagccgct gtaagataat 
     6901 caatccctcc gtggagagtg acccgttcct ggccatttcg tacgtgatga aagcagcagc 
     6961 taagctggcc gactacatag tggcctcgcc acacggcctc cgtcgcaaaa acgcgtaccc 
     7021 gtccgagata gtcgaggcgt tggcgacgca cgtaagcgat tcgctccatt cccgggaggt 
     7081 ggccgctgtt tacattccgt gcatcgatga ggaggttgta gagttggata cgctctacat 
     7141 cagctcgaat cgccattacc tggattcgat cgccgagcga ctcggtttga aggacggcaa 
     7201 tcaggttatg attagtcgca tgtttggcaa ggcgtcgtgt ggttccgagt gtcgtctgta 
     7261 ctcggagatc ccaaagaagt atctggacaa ttccagcgat gccagcggta cttccaacga 
     7321 ggattccagc catatcgtca agagccgcaa cgctcgcgcc tcgtacatct gtggtcagga 
     7381 tatccatggt gacgccatcc tcgccagcgt aggataccga tggaagtcgg acgacctgga 
     7441 ctacgatgat gtgacagtca acagtttcta caggatatgc tgcggcatgt ccccgaatgg 
     7501 tatatacaaa ataagcaggc aggccgtgat cgcggcgcag ctgcccttcg cctttactac 
     7561 gaagagcaat tggcgcggtc cgttgccccg tgagatcctg ggtctgttct gccaccagca 
     7621 ccgactggcg gaaccaattc tgtccagcag taccgcgccg gtgaagagtt tgtccgacat 
     7681 ctttcgcagt cacaagaagt tgaaggtaag cggtgttgac gacgcaaacg agaacttgtc 
     7741 ccgccagaag gaagatacgc ccggactggg tcacggcttc cgatgtgagg ttaagatttt 
     7801 taccaagagt caggacctgg tactcgaatg ctccccacgt aaattctacg agaaggagaa 
     7861 cgatgccatc cagaacgcct ccttgaaggc cttgctctgg ttcagcaagt tctttgccga 
     7921 cctggacgtt gacggcgagc aaagttgcga cactgatgat gatcaggata cgaagagcag 
     7981 tagcccaaac gtattcgccg ccccccccat cttgcagaag gagcactcct cggagagcaa 
     8041 gaacaccaat gtgctcagtg cggaaaagcg ggtccagtcc atcaccaatg gcagtgtcgt 
     8101 gagtatttgc tactccctca gcctggccgt cgatcccgag tattcgtccg atggcgaaag 
     8161 ccccagggag gataatgaaa gtaacgagga gatggaaagt gaatacagcg ccaactgtga 
     8221 gtcgagtgtg gagctgattg agtcgaacga ggagatcgag ttcgaggtcg gcaccggcag 
     8281 catgaacccg cacattgaga gcgaggtgac ccaaatgacc gttggtgagt acgccagctt 
     8341 tcgcatgaca ccgccagacg cggcagaggc actgatcctg gcagtgggca gcgacaccgt 
     8401 gcgaatccgc tccctgctga gcgagaggcc atgcctcaat tacaacatcc tgctgctggg 
     8461 cgttaaggga cccagtgagg agcgcatgga ggccgccttc ttcaagcccc ctctctcgaa 
     8521 gcaacgagtg gagtatgccc tgaagcatat tcgagagtcg agtgcatcga cgctggtgga 
     8581 ttttggctgt ggctccggaa gtctgttgga tagtctcctg gactacccaa caagcctgca 
     8641 aacgattata ggcgtggaca tctcgcctaa gggcctcgcc cgtgccgcga agatgctgca 
     8701 cgttaaattg aacaaggagg cgtgcaatgt taagagtgct actctgtacg atggaagcat 
     8761 tctcgagtta agggtgggcg cgccgaccca gctttc 
//
