LOCUS       Exported                6265 bp ds-DNA     circular SYN 01-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    FP1118-pCAG mito-RCaMP1h
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 1, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3630..4958
                     /organism="pRSET-RCaMP1h"
                     /mol_type="other DNA"
                     /label=source:pRSET-RCaMP1h
                     /note="source:pRSET-RCaMP1h"
     CDS             complement(803..1462)
                     /label=AmpR
                     /note="AmpR"
     misc_feature    1810..3421
                     /label=CAG promoter
                     /note="CAG promoter"
     misc_feature    3528..3614
                     /label=mito
                     /note="mito"
     CDS             3630..4958
                     /codon_start=1
                     /label=ORF frame 1
                     /note="ORF frame 1"
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSAINTEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTTYRSKKTVGN
                     IKMPAIHYVSHRLERLEESDNEMFVVQREHAVAKFVGLGGGGGTGGSMNSLIKENMRMK
                     VVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIK
                     YPKGIPDFFKQSFPEGFTWERVTRYEDGGVITVMQDTSLEDGCLVYHVQVRGVNFPSNG
                     AVMQKKTKGWEPTDSQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLIMMARKMKYTDSEEEIREAFGVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADSDGDGQVNYEEFVQMMTAK"
     misc_feature    3639..3656
                     /label=6xHIS
                     /note="6xHIS"
     misc_feature    3659..3677
                     /label=Xpress_fwd_primer
                     /note="Xpress_fwd_primer"
     misc_feature    3660..3692
                     /label=T7_leader
                     /note="T7_leader"
     misc_feature    3660..3692
                     /label=T7 Tag
                     /note="T7 Tag"
     misc_feature    3696..3719
                     /label=Xpress_EK
                     /note="Xpress_EK"
     primer_bind     complement(5556..5576)
                     /label=M13-rev
                     /note="M13-rev"
     misc_binding    complement(5582..5604)
                     /label=LacO
                     /note="LacO"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2221 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     2281 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2341 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2401 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2461 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2521 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     2581 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     2641 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     2701 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     2761 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2821 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2881 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     2941 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3001 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3061 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3121 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3181 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3241 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3301 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     3361 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3421 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3481 tgctggttat tgtgctgtct catcattttg gcaaagaatt gctcgagatg tccgtcctga
     3541 cgccgctgct gctgcggggc ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca
     3601 agatccattc gttgggggat ccaccggtca tgggttctca tcatcatcat catcatggta
     3661 tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg
     3721 atctcgccac catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca
     3781 gagctatagg tcggctgagc tcagcgatca acaccgaaat gatgtaccca gcggatggtg
     3841 gtctgcgtgg ttacactcac atggcgctga aagttgatgg cggcggtcac ctgtcctgtt
     3901 ctttcgtgac cacctaccgc tccaaaaaga ctgtcggcaa cattaagatg cctgccattc
     3961 attacgtcag ccaccgtctg gagcgcctgg aggagagcga taacgaaatg tttgtcgtac
     4021 agcgtgaaca cgcagttgcc aagtttgtgg gcctgggtgg tggcggcggt accggaggga
     4081 gcatgaactc cctgatcaag gagaacatgc gtatgaaagt ggttctggaa ggctccgtaa
     4141 acggccacca gttcaaatgc actggtgaag gcgaaggcaa cccgtatatg ggcacccaga
     4201 ctatgcgtat caaagtgatc gagggtggtc cgctgccgtt tgcgttcgac atcctggcga
     4261 cgtcctttat gtatggctcc cgtaccttca tcaaatatcc gaaaggcatc ccggatttct
     4321 ttaagcagtc cttcccggaa ggttttacct gggaacgtgt gacccgttac gaagacggcg
     4381 gcgtaattac cgttatgcaa gacacgtctc tggaggatgg ctgcctggtg tatcacgtgc
     4441 aggttcgcgg tgttaacttc ccgagcaatg gtgctgtaat gcaaaagaaa accaaaggtt
     4501 gggagcctac ggactcccaa ctgactgaag agcagatcgc agaatttaaa gaggctttct
     4561 ccctatttga caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc
     4621 ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg
     4681 ccgacggtga cggcacaatc gacttccctg agttcctgat tatgatggca agaaaaatga
     4741 aatacacaga cagtgaagaa gaaattagag aagcgttcgg cgtgtttgat aaggatggca
     4801 atggctacat cagtgcagca gagcttcgcc acgtgatgac aaaccttgga gagaagttaa
     4861 cagatgaaga ggttgatgaa atgatcaggg aagcagacag cgatggggat ggtcaggtaa
     4921 actacgaaga gtttgtacaa atgatgacag cgaagtaagc ggccgcaatt cactcctcag
     4981 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     5041 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     5101 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     5161 tgtctatcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     5221 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     5281 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     5341 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     5401 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     5461 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcaccg tcgaccagct
     5521 ggtcgacggt gcaccgtcga ccagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5581 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5641 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5701 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     5761 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5821 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5881 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     5941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6061 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6121 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6181 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6241 aaagaacatg tgagcaaaag gccag
//
